MysmA.00654.b

Putative acyl-CoA dehydrogenase

CENTER ID: MysmA.00654.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00654.b.A1.PW28723 Full length( MysmA.00654.b ) 1 385

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5072
RefSeq: YP_889445.1
UniProt: A0R2Q7

Orthologues for Rv3556c

Species % Identity % Coverage Clones Proteins Structures
avium 96 100 0 0 0
paratuberculosis 95 100 1 0 0
marinum 92 100 1 0 0
ulcerans 92 100 1 0 0
smegmatis 80 100 0 0 0
abscessus 79 100 0 0 0
avium 69 100 0 0 0
avium 51 100 0 0 0
marinum 51 100 1 0 0
ulcerans 51 100 1 0 0
thermoresistibile 51 100 1 0 0
smegmatis 50 100 0 1 0
avium 50 99 0 0 0
paratuberculosis 50 99 1 0 0
abscessus 49 100 1 0 0
smegmatis 49 100 0 0 0
avium 48 99 0 0 0
paratuberculosis 48 99 0 0 0
smegmatis 48 99 0 0 0
thermoresistibile 47 100 1 0 0
marinum 47 99 0 0 0
ulcerans 47 99 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEAVIVEAV RSPVGKRNGA LSGIHPAELS AQVLNGLVER AGVDPALVDD VIWGCVMQAG EQALDIARTA VLSAGWPETV PGVTVDRQCG SSQQSLHFAV AGVVAGHYDV VVAGGVESMS RTPMGSSLAN GGNPYGESFK ARYDKTPNQG VGAEMIAEQW GFSRTQLDEF SLRSHEKAAA AQDSGAFSDQ IVGIKTKDAD GNDTVVLEDG GIRRGGTIEA MAGIKPAFKE DGVIHAGNSS QISDGSAALL ITSAEKAKEL GLKPIAKVHT AVLAGADPVI MLTAPIPATQ KALKKSGLSL DQIGAFEVNE AFAPVPMAWL KDIGADEKKL NPNGGAIALG HPLGGSGARI LTTLLYHMRD NNIQYGLQTM CEGGGQANAT ILELL
NT Sequence
ATGGCTGAAG CCGTCATCGT CGAGGCCGTC CGCTCGCCTG TCGGCAAGCG CAACGGCGCG TTGTCGGGAA TCCACCCCGC CGAGCTGTCC GCCCAGGTCC TCAACGGCCT GGTGGAGCGC GCGGGGGTTG ATCCGGCACT GGTCGACGAC GTCATCTGGG GCTGCGTCAT GCAGGCCGGT GAACAGGCCC TCGACATCGC GCGCACCGCG GTGCTGAGCG CCGGCTGGCC CGAGACGGTC CCGGGTGTCA CCGTGGACCG CCAGTGCGGC TCCAGCCAGC AGTCGCTGCA CTTCGCCGTC GCCGGTGTCG TCGCGGGTCA CTACGACGTC GTCGTCGCCG GTGGTGTCGA GTCGATGTCG CGCACCCCGA TGGGCTCGTC GCTGGCCAAC GGCGGCAACC CCTACGGCGA GTCGTTCAAG GCGCGCTACG ACAAGACCCC GAACCAGGGT GTCGGCGCCG AGATGATCGC CGAGCAGTGG GGCTTCAGCC GTACCCAGCT CGACGAGTTC TCGCTGCGCT CGCACGAGAA GGCCGCCGCG GCCCAGGATT CGGGCGCGTT CTCCGACCAG ATCGTGGGCA TCAAGACCAA GGACGCCGAC GGCAACGACA CCGTCGTGCT CGAGGACGGC GGCATCCGCC GCGGCGGCAC CATCGAGGCG ATGGCCGGCA TCAAGCCCGC CTTCAAGGAG GACGGCGTGA TCCACGCCGG TAACTCCAGC CAGATCTCCG ACGGCTCGGC CGCCCTGCTG ATCACCTCGG CCGAGAAGGC CAAGGAGCTC GGTCTCAAGC CGATCGCCAA GGTGCACACC GCGGTTCTCG CAGGCGCCGA TCCGGTCATC ATGCTGACCG CGCCGATCCC GGCCACGCAG AAGGCCCTGA AGAAGTCGGG CCTGAGCCTC GACCAGATCG GTGCGTTCGA GGTCAACGAG GCGTTCGCGC CGGTTCCGAT GGCCTGGCTC AAGGACATCG GTGCCGACGA GAAGAAGCTG AACCCCAACG GCGGCGCCAT CGCCCTGGGC CACCCGCTCG GCGGTTCGGG TGCGCGCATC CTGACGACGC TGCTGTACCA CATGCGCGAC AACAACATTC AGTACGGCCT GCAGACCATG TGCGAGGGTG GCGGCCAGGC CAACGCGACC ATCCTGGAGC TTCTG
Details for MysmA.00654.b.A1.PW28723
PURIFICATION DATe: 5/3/2010
CONCENTRATION: 24.55mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 74
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAVIVEA VRSPVGKRNG ALSGIHPAEL SAQVLNGLVE RAGVDPALVD DVIWGCVMQA GEQALDIART AVLSAGWPET VPGVTVDRQC GSSQQSLHFA VAGVVAGHYD VVVAGGVESM SRTPMGSSLA NGGNPYGESF KARYDKTPNQ GVGAEMIAEQ WGFSRTQLDE FSLRSHEKAA AAQDSGAFSD QIVGIKTKDA DGNDTVVLED GGIRRGGTIE AMAGIKPAFK EDGVIHAGNS SQISDGSAAL LITSAXKAKE LGLKPIAKVH TAVLAXPIRS SC
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggctga agccgtcatc gtcgaggccg tccgctcgcc tgtcggcaag cgcaacggcg cgttgtcggg aatccacccc gccgagctgt ccgcccaggt cctcaacggc ctggtggagc gcgcgggggt tgatccggca ctggtcgacg acgtcatctg gggctgcgtc atgcaggccg gtgaacaggc cctcgacatc gcgcgcaccg cggtgctgag cgccggctgg cccgagacgg tcccgggtgt caccgtggac cgccagtgcg gctccagcca gcagtcgctg cacttcgccg tcgccggtgt cgtcgcgggt cactacgacg tcgtcgtcgc cggtggtgtc gagtcgatgt cgcgcacccc gatgggctcg tcgctggcca acggcggcaa cccctacggc gagtcgttca aggcgcgcta cgacaagacc ccgaaccagg gtgtcggcgc cgagatgatc gccgagcagt ggggcttcag ccgtacccag ctcgacgagt tctcgctgcg ctcgcacgag aaggccgccg cggcccagga ttcgggcgcg ttctccgacc agatcgtggg catcaagacc aaggacgccg acggcaacga caccgtcgtg ctcgaggacg gcggcatccg ccgcggcggc accatcgagg cgatggccgg catcaagccc gccttcaagg aggacggcgt gatccacgcc ggtaactcca gccagatctc cgacggctcg gccgccctgc tgatcacctc ggccganaag gccaaggagc tcggtctcaa gccgatcgcc aaggtgcaca ccgcggttct cgcnnngccg atccggtcat catgctgacn gcgccgatcc cngncacgca gaanncctga agaantcggg ctgancntcg accagatcgn ngcgttcgan gtcannnann nttcgcgncn ntncnatngn ctggnnnnnn gncatnnnnn nnannnnnnn nanctgaacc nnnggcggnn nnntnnncct ngnnnncnnn nnnnnngnnc ngnnnnnnnn nnnnnnnntn nnnntnngnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngn nnntnnnnnn nnnaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAVIVEA VRSPVGKRNG ALSGIHPAEL SAQVLNGLVE RAGVDPALVD DVIWGCVMQA GEQALDIART AVLSAGWPET VPGVTVDRQC GSSQQSLHFA VAGVVAGHYD VVVAGGVESM SRTPMGSSLA NGGNPYGESF KARYDKTPNQ GVGAEMIAEQ WGFSRTQLDE FSLRSHEKAA AAQDSGAFSD QIVGIKTKDA DGNDTVVLED GGIRRGGTIE AMAGIKPAFK EDGVIHAGNS SQISDGSAAL LITSAEKAKE LGLKPIAKVH TAVLAGADPV IMLTAPIPAT QKALKKSGLS LDQIGAFEVN EAFAPVPMAW LKDIGADEKK LNPNGGAIAL GHPLGGSGAR ILTTLLYHMR DNNIQYGLQT MCEGGGQANA TILELL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGAAGCC GTCATCGTCG AGGCCGTCCG CTCGCCTGTC GGCAAGCGCA ACGGCGCGTT GTCGGGAATC CACCCCGCCG AGCTGTCCGC CCAGGTCCTC AACGGCCTGG TGGAGCGCGC GGGGGTTGAT CCGGCACTGG TCGACGACGT CATCTGGGGC TGCGTCATGC AGGCCGGTGA ACAGGCCCTC GACATCGCGC GCACCGCGGT GCTGAGCGCC GGCTGGCCCG AGACGGTCCC GGGTGTCACC GTGGACCGCC AGTGCGGCTC CAGCCAGCAG TCGCTGCACT TCGCCGTCGC CGGTGTCGTC GCGGGTCACT ACGACGTCGT CGTCGCCGGT GGTGTCGAGT CGATGTCGCG CACCCCGATG GGCTCGTCGC TGGCCAACGG CGGCAACCCC TACGGCGAGT CGTTCAAGGC GCGCTACGAC AAGACCCCGA ACCAGGGTGT CGGCGCCGAG ATGATCGCCG AGCAGTGGGG CTTCAGCCGT ACCCAGCTCG ACGAGTTCTC GCTGCGCTCG CACGAGAAGG CCGCCGCGGC CCAGGATTCG GGCGCGTTCT CCGACCAGAT CGTGGGCATC AAGACCAAGG ACGCCGACGG CAACGACACC GTCGTGCTCG AGGACGGCGG CATCCGCCGC GGCGGCACCA TCGAGGCGAT GGCCGGCATC AAGCCCGCCT TCAAGGAGGA CGGCGTGATC CACGCCGGTA ACTCCAGCCA GATCTCCGAC GGCTCGGCCG CCCTGCTGAT CACCTCGGCC GAGAAGGCCA AGGAGCTCGG TCTCAAGCCG ATCGCCAAGG TGCACACCGC GGTTCTCGCA GGCGCCGATC CGGTCATCAT GCTGACCGCG CCGATCCCGG CCACGCAGAA GGCCCTGAAG AAGTCGGGCC TGAGCCTCGA CCAGATCGGT GCGTTCGAGG TCAACGAGGC GTTCGCGCCG GTTCCGATGG CCTGGCTCAA GGACATCGGT GCCGACGAGA AGAAGCTGAA CCCCAACGGC GGCGCCATCG CCCTGGGCCA CCCGCTCGGC GGTTCGGGTG CGCGCATCCT GACGACGCTG CTGTACCACA TGCGCGACAA CAACATTCAG TACGGCCTGC AGACCATGTG CGAGGGTGGC GGCCAGGCCA ACGCGACCAT CCTGGAGCTT CTGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA