MymaA.00654.c

Acetyl-CoA acetyltransferase FadA6_1

CENTER ID: MymaA.00654.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00654.c.A1.GE34946 Full length( MymaA.00654.c ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5405
RefSeq: YP_001853304.1
UniProt: B2HIY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEAYVIDAV RTAVGKRGGA LAGVHPVDLG ALGWRGLLDR VDVDPAAVDD VIAGCVDAIG GQAGNIARLS WLAAGYPEEV PGVTVDRQCG SSQQAISFGA QAIMSGTADL IVAGGMQNMS QIPISSAMIV GEQFGFSSPT NESKQWLHRY GDQEISQFRG SEMIAEKWNL SREEMEQYAL TSHARAFAAI RGGRFDNEIL SVETESGPFR VDEGPRESSL EKMAGLKTLV EGGRLTAAMA SQISDGASAV LLASEQAVKD HGLTPRARIH HISARAADPV FMLTGPIPAT RHALEKTGLS IDDIATVEIN EAFAPVVLAW LKEIKADPEK VNPNGGAIAL GHPLGATGAK LFTTMLNELD RVGGRYGLQT MCEGGGTANV TIIERL
NT Sequence
ATGCCGGAGG CGTACGTCAT CGACGCTGTA CGTACCGCGG TGGGCAAGCG TGGCGGAGCG TTGGCGGGGG TACATCCCGT CGACCTGGGT GCCTTGGGGT GGCGGGGACT GCTGGATCGA GTTGACGTCG ACCCGGCCGC GGTCGACGAC GTGATCGCGG GCTGTGTCGA TGCCATCGGC GGTCAGGCCG GCAATATCGC ACGGTTGTCC TGGCTTGCGG CGGGATATCC CGAAGAAGTG CCAGGTGTCA CCGTGGACCG CCAATGCGGC TCCAGCCAGC AGGCGATCTC CTTTGGTGCG CAAGCGATCA TGTCCGGAAC GGCCGACCTC ATCGTTGCCG GTGGCATGCA GAACATGAGC CAGATCCCGA TCTCGTCGGC AATGATCGTC GGTGAACAAT TCGGCTTTAG CTCGCCGACC AACGAATCCA AGCAGTGGCT GCACCGGTAC GGGGATCAGG AGATCTCTCA GTTCCGCGGT TCGGAGATGA TCGCCGAGAA GTGGAATTTG TCGCGTGAAG AGATGGAGCA GTATGCGCTG ACCAGCCACG CGCGTGCGTT CGCGGCGATC AGGGGTGGCC GCTTCGATAA CGAAATCCTT AGTGTGGAAA CGGAATCCGG GCCCTTCCGG GTCGACGAAG GGCCGCGCGA GTCATCGCTG GAGAAGATGG CTGGCTTGAA GACCCTGGTC GAGGGGGGAC GGCTTACCGC CGCGATGGCC AGCCAAATCT CCGACGGTGC CAGCGCGGTG CTGCTGGCTT CCGAACAGGC GGTCAAGGAT CACGGCCTGA CTCCGCGCGC TCGCATCCAC CACATCAGTG CGCGCGCCGC CGACCCGGTG TTCATGCTCA CCGGCCCCAT CCCGGCGACC CGCCATGCGC TGGAAAAGAC CGGCCTGTCC ATCGATGACA TCGCCACGGT AGAGATCAAT GAGGCATTCG CGCCGGTGGT TTTGGCGTGG CTCAAGGAAA TCAAGGCCGA TCCCGAGAAG GTCAACCCCA ACGGCGGTGC CATCGCTCTC GGTCATCCGC TGGGTGCCAC CGGGGCCAAG CTGTTCACCA CCATGCTCAA TGAACTCGAC CGGGTCGGTG GGCGTTACGG GCTACAGACG ATGTGTGAAG GCGGGGGCAC CGCCAACGTC ACGATCATCG AGCGGCTA
Details for MymaA.00654.c.A1.GE34946
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAYVIDA VRTAVGKRGG ALAGVHPVDL GALGWRGLLD RVDVDPAAVD DVIAGCVDAI GGQAGNIARL SWLAAGYPEE VPGVTVDRQC GSSQQAISFG AQAIMSGTAD LIVAGGMQNM SQIPISSAMI VGEQFGFSSP TNESKQWLHR YGDQEISQFR GSEMIAEKWN LSREEMEQYA LTSHARAFAA IRGGRFDNEI LSVETESGPF RVDEXPRESS LEKXAGLKXL VEGGRLTAAM ASQISXGASA VLLASEQAVK DHGLTPRAXI HHIS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccgg aggcgtacgt catcgacgct gtacgtaccg cggtgggcaa gcgtggcgga gcgttggcgg gggtacatcc cgtcgacctg ggtgccttgg ggtggcgggg actgctggat cgagttgacg tcgacccggc cgcggtcgac gacgtgatcg cgggctgtgt cgatgccatc ggcggtcagg ccggcaatat cgcacggttg tcctggcttg cggcgggata tcccgaagaa gtgccaggtg tcaccgtgga ccgccaatgc ggctccagcc agcaggcgat ctcctttggt gcgcaagcga tcatgtccgg aacggccgac ctcatcgttg ccggtggcat gcagaacatg agccagatcc cgatctcgtc ggcaatgatc gtcggtgaac aattcggctt tagctcgccg accaacgaat ccaagcagtg gctgcaccgg tacggggatc aggagatctc tcagttccgc ggttcggaga tgatcgccga gaagtggaat ttgtcgcgtg aagagatgga gcagtatgcg ctgaccagcc acgcgcgtgc gttcgcggcg atcaggggtg gccgcttcga taacgaaatc cttagtgtgg aaacggaatc cgggcccttc cgggtcgacg aanggccgcg cgagtcatcg ctggagaaga nggctggctt gaagancctg gtcgaggggg gacggcttac cgccgcgatg gccagccaaa tctccnacgg tgccagcgcg gtgctgctgg cttccgaaca ggcggtcaag gatcacggcc tgactccgcg cgctcncatc caccacatca gtg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAYVIDA VRTAVGKRGG ALAGVHPVDL GALGWRGLLD RVDVDPAAVD DVIAGCVDAI GGQAGNIARL SWLAAGYPEE VPGVTVDRQC GSSQQAISFG AQAIMSGTAD LIVAGGMQNM SQIPISSAMI VGEQFGFSSP TNESKQWLHR YGDQEISQFR GSEMIAEKWN LSREEMEQYA LTSHARAFAA IRGGRFDNEI LSVETESGPF RVDEGPRESS LEKMAGLKTL VEGGRLTAAM ASQISDGASA VLLASEQAVK DHGLTPRARI HHISARAADP VFMLTGPIPA TRHALEKTGL SIDDIATVEI NEAFAPVVLA WLKEIKADPE KVNPNGGAIA LGHPLGATGA KLFTTMLNEL DRVGGRYGLQ TMCEGGGTAN VTIIER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggaggcg tacgtcatcg acgctgtacg taccgcggtg ggcaagcgtg gcggagcgtt ggcgggggta catcccgtcg acctgggtgc cttggggtgg cggggactgc tggatcgagt tgacgtcgac ccggccgcgg tcgacgacgt gatcgcgggc tgtgtcgatg ccatcggcgg tcaggccggc aatatcgcac ggttgtcctg gcttgcggcg ggatatcccg aagaagtgcc aggtgtcacc gtggaccgcc aatgcggctc cagccagcag gcgatctcct ttggtgcgca agcgatcatg tccggaacgg ccgacctcat cgttgccggt ggcatgcaga acatgagcca gatcccgatc tcgtcggcaa tgatcgtcgg tgaacaattc ggctttagct cgccgaccaa cgaatccaag cagtggctgc accggtacgg ggatcaggag atctctcagt tccgcggttc ggagatgatc gccgagaagt ggaatttgtc gcgtgaagag atggagcagt atgcgctgac cagccacgcg cgtgcgttcg cggcgatcag gggtggccgc ttcgataacg aaatccttag tgtggaaacg gaatccgggc ccttccgggt cgacgaaggg ccgcgcgagt catcgctgga gaagatggct ggcttgaaga ccctggtcga ggggggacgg cttaccgccg cgatggccag ccaaatctcc gacggtgcca gcgcggtgct gctggcttcc gaacaggcgg tcaaggatca cggcctgact ccgcgcgctc gcatccacca catcagtgcg cgcgccgccg acccggtgtt catgctcacc ggccccatcc cggcgacccg ccatgcgctg gaaaagaccg gcctgtccat cgatgacatc gccacggtag agatcaatga ggcattcgcg ccggtggttt tggcgtggct caaggaaatc aaggccgatc ccgagaaggt caaccccaac ggcggtgcca tcgctctcgg tcatccgctg ggtgccaccg gggccaagct gttcaccacc atgctcaatg aactcgaccg ggtcggtggg cgttacgggc tacagacgat gtgtgaaggc gggggcaccg ccaacgtcac gatcatcgag cggaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa