MythA.00654.b

Acetyl-CoA acetyltransferase (A0R4Z5 homolog)

CENTER ID: MythA.00654.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00654.b.A1.GE28747 Full length( MythA.00654.b ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.1255
RefSeq: WP_003924768.1
UniProt: G7CE56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAMTEAYIVD AVRTPVGKRN GGLSGAHPAD MAAHVIKTLV GRHDFDPAAI DDVIFGCLDN IGSQAGDIAR TAALAAGLPE SVPGVTIDRQ CGSAQQAVHF AAQGVMSGTV DLIVAGGVQK MSQFPILSAF AAGEPPRXLR RHRSMDRMRG LAGALRRPGD LPVPRRRDDR RALEAVPRGQ REVRAGEPPA RRGGHRSRLL RQGDHAVRRR DRRRGTAPGH LPGEDGEPAX XXXXXXXXXX XXXXXXXXXX XXXAAALLVA SKDAVDRFNL TPRARIHHMS VRGADPVMML TAPIPATQHA LKRAGMSIDD IDAVEINEAF APVVLAWLAE LGADPARVNP NGGAIALGHP IGCTGARLMT SLLHHLERTG GRYGLQTMCE GGGQANVTII ERL
NT Sequence
ATGGCCATGA CCGAAGCCTA TATCGTTGAT GCTGTCCGAA CCCCCGTCGG GAAACGCAAC GGCGGACTCT CCGGTGCCCA CCCGGCCGAC ATGGCCGCCC ACGTGATCAA GACCCTGGTG GGCCGCCACG ACTTCGATCC CGCCGCGATC GACGACGTCA TCTTCGGGTG TCTGGACAAC ATCGGCTCCC AGGCCGGCGA CATCGCCCGC ACCGCAGCAC TGGCGGCCGG GTTGCCGGAA TCCGTGCCCG GCGTGACCAT CGACCGGCAG TGCGGCTCTG CGCAGCAGGC CGTCCACTTC GCCGCGCAGG GTGTGATGAG CGGCACGGTC GACCTGATTG TCGCCGGCGG CGTCCAGAAG ATGAGCCAGT TCCCCATCCT GAGCGCGTTC GCGGCCGGCG AACCCCCCCG CNNCCTACGG CGCCACCGAT CCATGGACCG GATGCGAGGG CTGGCAGGCG CGTTACGGCG ACCAGGAGAT CTCCCAGTTC CGCGGCGCCG AGATGATCGC CGAGCACTGG AAGCTGTCCC GCGAGGACAA CGAGAAGTTC GCGCTGGAGA GCCACCGGCG CGCCGTGGCG GCCATCGAAG CCGGCTACTT CGACAAGGAG ATCACGCCGT ACGCCGACGC GACCGTCGAC GAGGGACCGC GCCCGGACAC CTCCCTGGAG AAGATGGCGA GCCTGCCCNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGCG CCGCCGCCCT GCTGGTGGCG TCGAAGGATG CGGTCGACCG GTTCAATCTC ACCCCGCGGG CCCGTATCCA TCACATGTCG GTCCGCGGCG CCGACCCGGT GATGATGCTC ACCGCACCGA TTCCGGCCAC CCAGCACGCC CTCAAGCGCG CCGGTATGTC GATCGACGAC ATCGATGCGG TCGAGATCAA CGAGGCGTTC GCACCGGTGG TGCTGGCCTG GCTGGCCGAA CTCGGCGCCG ACCCGGCCCG GGTCAATCCG AACGGAGGCG CCATCGCGCT GGGTCATCCG ATCGGCTGCA CCGGAGCCCG GCTGATGACC TCACTGCTGC ACCATCTCGA GCGCACCGGC GGCCGGTACG GTCTGCAGAC CATGTGTGAG GGCGGTGGTC AGGCCAACGT CACCATAATC GAGAGATTG
Details for MythA.00654.b.A1.GE28747
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 76
PERCENT COVERAGE: 85
Validated AA Sequence
MAAHVIKTLV GRHDFDPAAI DDXIFGCLDN IGSQAGDIAR TAALAAGLPE SVPGVTIDRQ CGSAQQAVHF AAQGVMSGTV DLIVAGGVQK MSQFPILSAF AAGEPYGATD PWTGCEGWQA RYGDQEISQF RGAEMIAEHW KLSREDNEKF ALESHRRAVA AIEAGYFDKE ITPYADATVD EGPRPDTSLE KMASLPTLIE GGVLTAAVAS QISDGAAALL VASKDAVDRF NLTPRARIHH MSVRGADPVM MLTAPIPATQ HALKRAGMSI DDIDAVEINE AFAPVVLAWL AELGADPARV NPNGGAIALG HPIGCTGARL MTSLLHHLER TGGRYGLQTM CEGGGQANVT IIERL
Validated NT Sequence
ttattacaat ctctcgatta tggtgacgtt ggcctgacca ccgccctcac acatggtctg cagaccgtac cggccgccgg tgcgctcgag atggtgcagc agtgaggtca tcagccgggc tccggtgcag ccgatcggat gacccagcgc gatggcgcct ccgttcggat tgacccgggc cgggtcggcg ccgagttcgg ccagccaggc cagcaccacc ggtgcgaacg cctcgttgat ctcgaccgca tcgatgtcgt cgatcgacat accggcgcgc ttgagggcgt gctgggtggc cggaatcggt gcggtgagca tcatcaccgg gtcggcgccg cggaccgaca tgtgatggat acgggcccgc ggggtgagat tgaaccggtc gaccgcatcc ttcgacgcca ccagcagggc ggcggcgccg tccgagatct ggctggccac cgcggcggtg agcacaccgc cctcgatcag cgtgggcagg ctcgccatct tctccaggga ggtgtccggg cgcggtccct cgtcgacggt cgcgtcggcg tacggcgtga tctccttgtc gaagtagccg gcttcgatgg ccgccacggc gcgccggtgg ctctccagcg cgaacttctc gttgtcctcg cgggacagct tccagtgctc ggcgatcatc tcggcgccgc ggaactggga gatctcctgg tcgccgtaac gcgcctgcca gccctcgcat ccggtccatg gatcggtggc gccgtagggt tcgccggccg cgaacgcgct caggatgggg aactggctca tcttctggac gccgccggcg acaatcaggt cgaccgtgcc gctcatcaca ccctgcgcgg cgaagtggac ggcctgctgc gcagagccgc actgccggtc gatggtcacg ccgggcacgg attccggcaa cccggccgcc agtgctgcgg tgcgggcgat gtcgccggcc tgggagccga tgttgtccag acacccgaag atgangtcgt cgatcgcggc gggatcgaag tcgtggcggc ccaccagggt cttgatcacg tgggcggcca tgtcggccgg gtgggcaccg gagagtccgc cgttgcgttt cccgacgggg gttcggacag catcaacgat a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAMTEAYIV DAVRTPVGKR NGGLSGAHPA DMAAHVIKTL VGRHDFDPAA IDDVIFGCLD NIGSQAGDIA RTAALAAGLP ESVPGVTIDR QCGSAQQAVH FAAQGVMSGT VDLIVAGGVQ KMSQFPILSA FAAGEPPRXL RRHRSMDRMR GLAGALRRPG DLPVPRRRDD RRALEAVPRG QREVRAGEPP ARRGGHRSRL LRQGDHAVRR RDRRRGTAPG HLPGEDGEPA XXXXXXXXXX XXXXXXXXXX XXXXAAALLV ASKDAVDRFN LTPRARIHHM SVRGADPVMM LTAPIPATQH ALKRAGMSID DIDAVEINEA FAPVVLAWLA ELGADPARVN PNGGAIALGH PIGCTGARLM TSLLHHLERT GGRYGLQTMC EGGGQANVTI IERL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccatgacc gaagcctata tcgttgatgc tgtccgaacc cccgtcggga aacgcaacgg cggactctcc ggtgcccacc cggccgacat ggccgcccac gtgatcaaga ccctggtggg ccgccacgac ttcgatcccg ccgcgatcga cgacgtcatc ttcgggtgtc tggacaacat cggctcccag gccggcgaca tcgcccgcac cgcagcactg gcggccgggt tgccggaatc cgtgcccggc gtgaccatcg accggcagtg cggctctgcg cagcaggccg tccacttcgc cgcgcagggt gtgatgagcg gcacggtcga cctgattgtc gccggcggcg tccagaagat gagccagttc cccatcctga gcgcgttcgc ggccggcgaa cccccccgcn ncctacggcg ccaccgatcc atggaccgga tgcgagggct ggcaggcgcg ttacggcgac caggagatct cccagttccg cggcgccgag atgatcgccg agcactggaa gctgtcccgc gaggacaacg agaagttcgc gctggagagc caccggcgcg ccgtggcggc catcgaagcc ggctacttcg acaaggagat cacgccgtac gccgacgcga ccgtcgacga gggaccgcgc ccggacacct ccctggagaa gatggcgagc ctgcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgcc gccgccctgc tggtggcgtc gaaggatgcg gtcgaccggt tcaatctcac cccgcgggcc cgtatccatc acatgtcggt ccgcggcgcc gacccggtga tgatgctcac cgcaccgatt ccggccaccc agcacgccct caagcgcgcc ggtatgtcga tcgacgacat cgatgcggtc gagatcaacg aggcgttcgc accggtggtg ctggcctggc tggccgaact cggcgccgac ccggcccggg tcaatccgaa cggaggcgcc atcgcgctgg gtcatccgat cggctgcacc ggagcccggc tgatgacctc actgctgcac catctcgagc gcaccggcgg ccggtacggt ctgcagacca tgtgtgaggg cggtggtcag gccaacgtca ccataatcga gagattgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa