MyulA.00654.d

Acetyl-CoA acetyltransferase FadA6_3

CENTER ID: MyulA.00654.d
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00654.d.A1.GE29722 Full length( MyulA.00654.d ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.1197
RefSeq: YP_905060.1
UniProt: A0PMM0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEAVIVEAV RSPVGKRNGA LSGVHPADLS AQVLNGLADK AGIDPEIVDD VIWGCVMQAG EQALDIARTS LLTAGWPETV PGVTVDRQCG SSQQSVHFAA AGVVAGHYDV VVAGGVESMS RTPMGSSLAN GGRPYSQTFL ERYDGRIPNQ GLGAEMIAEQ WGLSRTMLDE FSLMSHEKAA AAQDSGAFDD QIVGIKDQDG NPVLKDEGIR RGTPMEKMAA LKPAFKEDGV IHAGNSSQIS DGSAALLFMS AEKAKSLGMK PIAKVHTAVL AGADPVIMLT APIPATQKVL KRSGLSIGDI GAYEVNEAFA PVPLAWLCDI GADENRLNPN GGAIALGHPL GGSGARVMTT LLYHMRDNGI RYGLQTMCEG GGQANATVLE LL
NT Sequence
ATGGCTGAAG CCGTCATCGT AGAAGCGGTG CGCTCACCGG TCGGCAAGCG CAATGGCGCG CTATCCGGCG TACACCCCGC GGATCTATCT GCGCAGGTTC TCAACGGACT GGCTGACAAG GCCGGCATCG ACCCGGAGAT CGTGGATGAC GTCATCTGGG GCTGCGTCAT GCAAGCTGGG GAGCAGGCGC TCGATATCGC CCGCACCTCG CTGCTGACGG CCGGCTGGCC CGAAACGGTC CCGGGCGTGA CGGTCGACCG CCAGTGCGGT TCGAGTCAGC AGTCGGTGCA CTTCGCCGCG GCTGGCGTGG TGGCCGGGCA CTATGACGTC GTCGTCGCCG GCGGGGTGGA GTCGATGTCG CGAACCCCTA TGGGTTCTTC GCTGGCCAAT GGTGGGCGGC CGTATTCGCA GACCTTCCTG GAACGCTATG ACGGCCGCAT CCCTAACCAG GGCCTTGGTG CCGAGATGAT TGCCGAGCAG TGGGGATTGA GCCGAACCAT GCTCGACGAG TTCTCCTTGA TGTCGCACGA AAAGGCAGCT GCCGCACAAG ATTCCGGGGC ATTCGATGAT CAGATCGTCG GCATCAAGGA CCAGGATGGC AACCCCGTAC TCAAGGATGA AGGTATCCGG CGCGGAACGC CGATGGAAAA GATGGCGGCG CTGAAGCCGG CGTTCAAGGA AGACGGCGTG ATCCATGCCG GTAACTCGTC GCAGATTTCC GATGGTTCGG CGGCACTGTT GTTCATGTCC GCCGAAAAGG CTAAGTCGCT GGGGATGAAG CCGATTGCCA AGGTACACAC CGCGGTACTT GCGGGGGCCG ACCCGGTGAT CATGCTGACC GCGCCGATCC CGGCAACCCA GAAGGTGCTC AAGCGATCCG GGCTCAGTAT TGGCGACATC GGGGCATACG AGGTCAACGA GGCATTCGCT CCGGTTCCGC TGGCCTGGCT GTGTGACATC GGCGCCGACG AGAACCGGCT CAACCCCAAC GGGGGTGCGA TTGCGTTGGG TCACCCGCTG GGAGGATCGG GTGCTCGGGT GATGACCACG CTGCTGTACC ACATGCGGGA CAACGGAATT CGCTATGGTT TACAGACGAT GTGCGAGGGT GGTGGTCAAG CCAACGCCAC TGTCTTGGAG CTCTTG
Details for MyulA.00654.d.A1.GE29722
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAVIVEA VRSPVGKRNG ALSGVHPADL SAQVLNGLAD KAGIDPEIVD DVIWGCVMQA GEQALDIART SLLTAGWPET VPGVTVDRQC GSSQQSVHFA AAGVVAGHYD VVVAGGVESM SRTPMGSSLA NGGRPYSQTF LERYDGRIPN QGLGAEMIAE QWGLSRTMLD EFSLMSHEKA AAAQDSGAFD DQIVGIKDQD GNPVLKDEGI RRGTPMEKMA ALKPAFKEDG VIHAGNSSXI SDGSAALLFM SXEXAKSLGM KPIAXXHTAV LAGGRPXDHA DRADPXXPXX XKRSXXIXXX GHTXXXXXXX XPXXXXXXXX XXXXXSXPGX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg aagccgtcat cgtagaagcg gtgcgctcac cggtcggcaa gcgcaatggc gcgctatccg gcgtacaccc cgcggatcta tctgcgcagg ttctcaacgg actggctgac aaggccggca tcgacccgga gatcgtggat gacgtcatct ggggctgcgt catgcaagct ggggagcagg cgctcgatat cgcccgcacc tcgctgctga cggccggctg gcccgaaacg gtcccgggcg tgacggtcga ccgccagtgc ggttcgagtc agcagtcggt gcacttcgcc gcggctggcg tggtggccgg gcactatgac gtcgtcgtcg ccggcggggt ggagtcgatg tcgcgaaccc ctatgggttc ttcgctggcc aatggtgggc ggccgtattc gcagaccttc ctggaacgct atgacggccg catccctaac cagggccttg gtgccgagat gattgccgag cagtggggat tgagccgaac catgctcgac gagttctcct tgatgtcgca cgaaaaggca gctgccgcac aagattccgg ggcattcgat gatcagatcg tcggcatcaa ggaccaggat ggcaaccccg tactcaagga tgaaggnatc cggcgcggaa cgccgatgga aaagatggcg gcgctgaagc cggcgttcaa ggaagacggc gtgatccatg ccggtaactc gtcgcanatt tccgatggtt cggcggcnct gttgttcatg tccgncgaaa angctaagtc gctggggatg aagccnattg ccaangnaca caccgcggta cttgcggggg gccgaccnng tgatcatgct gaccgcgccg atcccngcnn cccagannng ntcaagcgat ccgnntnaat tnncnacntn gggcatacna gncnannann attnnnnncn ntnccnntgn nnngnntnnn nnncnnnnnn ncnannannn cnnnntcnnc nccnggnncn ntnnnntnnn gnncnncnnn nnnnnnnngg nnnnnnnnnn nagnacnnnn nnnntnnnnn g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAVIVEA VRSPVGKRNG ALSGVHPADL SAQVLNGLAD KAGIDPEIVD DVIWGCVMQA GEQALDIART SLLTAGWPET VPGVTVDRQC GSSQQSVHFA AAGVVAGHYD VVVAGGVESM SRTPMGSSLA NGGRPYSQTF LERYDGRIPN QGLGAEMIAE QWGLSRTMLD EFSLMSHEKA AAAQDSGAFD DQIVGIKDQD GNPVLKDEGI RRGTPMEKMA ALKPAFKEDG VIHAGNSSQI SDGSAALLFM SAEKAKSLGM KPIAKVHTAV LAGADPVIML TAPIPATQKV LKRSGLSIGD IGAYEVNEAF APVPLAWLCD IGADENRLNP NGGAIALGHP LGGSGARVMT TLLYHMRDNG IRYGLQTMCE GGGQANATVL ELL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgaagcc gtcatcgtag aagcggtgcg ctcaccggtc ggcaagcgca atggcgcgct atccggcgta caccccgcgg atctatctgc gcaggttctc aacggactgg ctgacaaggc cggcatcgac ccggagatcg tggatgacgt catctggggc tgcgtcatgc aagctgggga gcaggcgctc gatatcgccc gcacctcgct gctgacggcc ggctggcccg aaacggtccc gggcgtgacg gtcgaccgcc agtgcggttc gagtcagcag tcggtgcact tcgccgcggc tggcgtggtg gccgggcact atgacgtcgt cgtcgccggc ggggtggagt cgatgtcgcg aacccctatg ggttcttcgc tggccaatgg tgggcggccg tattcgcaga ccttcctgga acgctatgac ggccgcatcc ctaaccaggg ccttggtgcc gagatgattg ccgagcagtg gggattgagc cgaaccatgc tcgacgagtt ctccttgatg tcgcacgaaa aggcagctgc cgcacaagat tccggggcat tcgatgatca gatcgtcggc atcaaggacc aggatggcaa ccccgtactc aaggatgaag gtatccggcg cggaacgccg atggaaaaga tggcggcgct gaagccggcg ttcaaggaag acggcgtgat ccatgccggt aactcgtcgc agatttccga tggttcggcg gcactgttgt tcatgtccgc cgaaaaggct aagtcgctgg ggatgaagcc gattgccaag gtacacaccg cggtacttgc gggggccgac ccggtgatca tgctgaccgc gccgatcccg gcaacccaga aggtgctcaa gcgatccggg ctcagtattg gcgacatcgg ggcatacgag gtcaacgagg cattcgctcc ggttccgctg gcctggctgt gtgacatcgg cgccgacgag aaccggctca accccaacgg gggtgcgatt gcgttgggtc acccgctggg aggatcgggt gctcgggtga tgaccacgct gctgtaccac atgcgggaca acggaattcg ctatggttta cagacgatgt gcgagggtgg tggtcaagcc aacgccactg tcttggagct cttgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa