MyulA.00781.c

Acetyl-CoA acetyltransferase FadA6_4

CENTER ID: MyulA.00781.c
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00781.c.A1.GE29740 Full length( MyulA.00781.c ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3006
RefSeq: YP_906376.1
UniProt: A0PRD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQSRDAVIV GAVRTPIGKG KANGALHQVL PADLLAHSLR ELAARTGVDP VQVDDVIAGA VTQVGDQAVN IARNALLGAG FPESVPGVTI DRQCGSSQQA ISFAAQGVLA GAYDLVIAGG VESMSRVPMG TSVLPGSNPF GPDMNNRYPD GLVPQGISAE LIAARWGLSR TQLDEFSAAS HDKAARATKD GLFDNELIPI AGLTHDEIIR PGATVDTLAA LRPAFYSDAI KERFPQINWS ITPGNSSPLS DSSAAVMITS SDVARKLGLR PLARIHTTTV VGSDPLYMLT GVIAATEKAL RRAGLALRDI DLFEVNEAFA PVVLAWAKDT GADLAKTNVN GGAIAIGHPL GASGARIMTT LVNASEQRGG RYGLQTMCEG GGMANATIIE RLG
NT Sequence
ATGTCCCAGA GTCGCGACGC CGTCATCGTC GGCGCCGTCC GCACCCCCAT CGGCAAGGGC AAGGCCAACG GCGCACTGCA CCAGGTACTA CCCGCCGACC TGCTGGCGCA TAGCCTGCGC GAACTGGCGG CCCGAACCGG CGTCGACCCG GTACAAGTCG ACGATGTGAT CGCCGGCGCG GTGACCCAGG TCGGCGATCA GGCGGTCAAC ATCGCACGCA ATGCGCTGCT GGGTGCCGGA TTCCCGGAAT CGGTTCCCGG GGTCACCATC GACCGGCAAT GCGGCAGCAG CCAGCAGGCC ATCAGCTTTG CCGCCCAGGG CGTGCTGGCC GGTGCTTACG ATCTGGTGAT CGCCGGCGGC GTTGAATCGA TGAGCCGCGT CCCGATGGGC ACCTCGGTGC TGCCCGGAAG CAACCCGTTC GGCCCGGACA TGAACAACCG CTACCCCGAC GGGCTGGTCC CCCAGGGCAT CAGCGCCGAG CTGATTGCGG CACGGTGGGG CTTGTCGCGC ACCCAACTCG ACGAGTTCTC CGCAGCCAGC CATGACAAGG CCGCCCGCGC CACCAAGGAC GGACTCTTCG ACAACGAGCT GATCCCGATC GCCGGGCTCA CCCACGACGA GATCATCCGC CCCGGCGCCA CCGTCGATAC GCTCGCCGCG CTGCGGCCGG CGTTCTACAG CGATGCGATC AAAGAGCGCT TCCCGCAGAT CAACTGGTCG ATCACCCCGG GTAACTCGTC ACCGTTGTCC GACAGCAGCG CCGCGGTCAT GATCACCAGC AGCGACGTCG CCCGGAAGCT GGGCCTGCGC CCGTTGGCGC GCATTCACAC CACCACGGTG GTGGGTTCGG ATCCGCTCTA CATGCTGACC GGGGTCATCG CGGCCACCGA AAAGGCGCTA CGCCGCGCCG GGTTGGCCCT GCGCGACATC GACCTGTTCG AGGTCAACGA GGCCTTCGCC CCGGTCGTGC TGGCCTGGGC GAAGGACACC GGGGCGGATC TGGCCAAGAC CAACGTCAAC GGCGGCGCAA TCGCCATCGG CCACCCGTTG GGCGCCAGCG GTGCGCGCAT CATGACCACG CTGGTCAACG CTTCGGAGCA GCGCGGTGGC CGCTACGGCC TGCAGACCAT GTGCGAGGGC GGCGGCATGG CCAACGCGAC CATCATCGAG CGACTGGGG
Details for MyulA.00781.c.A1.GE29740
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 83
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQSRDAVI VGAVRTPIGK GKANGALHQV LPADLLAHSL RELAARTGVD PVQVDDVIAG AVTQVGDQAV NIARNALLGA GFPESVPGVT IDRQCGSSQQ AISFAAQGVL AGAYDLVIAG GVESMSRVPM GTSVLPGSNP FGPDMNNRYP DGLVPQGISA ELIAARWGLS RTQLDEFSAA SHDKAARATK DGLFDNELIP IAGLTHDEII RPGATVDTLA ALRPAFYSDA IKERFPQINW SITPGNSSPL SDSSAAVMIT SSDVARKLGL RPLARIHTTX VVGSXPLYML TXVIAXRKGA TXXGLXLXXI XXXXVNXXXX PXXLXXXXXX XXXXXXXXXX XXXXPXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttgtttn nctttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgtcc cagagtcgcg acgccgtcat cgtcggcgcc gtccgcaccc ccatcggcaa gggcaaggcc aacggcgcac tgcaccaggt actacccgcc gacctgctgg cgcatagcct gcgcgaactg gcggcccgaa ccggcgtcga cccggtacaa gtcgacgatg tgatcgccgg cgcggtgacc caggtcggcg atcaggcggt caacatcgca cgcaatgcgc tgctgggtgc cggattcccg gaatcggttc ccggggtcac catcgaccgg caatgcggca gcagccagca ggccatcagc tttgccgccc agggcgtgct ggccggtgct tacgatctgg tgatcgccgg cggcgttgaa tcgatgagcc gcgtcccgat gggcacctcg gtgctgcccg gaagcaaccc gttcggcccg gacatgaaca accgctaccc cgacgggctg gtcccccagg gcatcagcgc cgagctgatt gcggcacggt ggggcttgtc gcgcacccaa ctcgacgagt tctccgcagc cagccatgac aaggccgccc gcgccaccaa ggacggactc ttcgacaacg agctgatccc gatcgccggg ctcacccacg acgagatcat ccgccccggc gccaccgtcg atacgctcgc cgcgctgcgg ccggcgttct acagcgatgc gatcaaagag cgcttcccgc agatcaactg gtcgatcacc ccgggtaact cgtcaccgtt gtccgacagc agcgccgcgg tcatgatcac cagcagcgac gtcgcccgga agctgggcct gcgcccgttg gcgcgcattc acaccaccnc ggtggtgggt tcnnatccgc tctacatgct gaccngggtc atcgcggnnc gaaaaggngc tacncnngnn gggttggncc tgcnnnncat cganntgntc nnngtcaacn nngnnnnnnc cccngncnng ctnnnnnggn nnnanncnng gnnnnnnngn nnannnnnna nggnggnnca nnnncntnng nnnccnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nnntnnnnnn nnnnnnnnnn nnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQSRDAVI VGAVRTPIGK GKANGALHQV LPADLLAHSL RELAARTGVD PVQVDDVIAG AVTQVGDQAV NIARNALLGA GFPESVPGVT IDRQCGSSQQ AISFAAQGVL AGAYDLVIAG GVESMSRVPM GTSVLPGSNP FGPDMNNRYP DGLVPQGISA ELIAARWGLS RTQLDEFSAA SHDKAARATK DGLFDNELIP IAGLTHDEII RPGATVDTLA ALRPAFYSDA IKERFPQINW SITPGNSSPL SDSSAAVMIT SSDVARKLGL RPLARIHTTT VVGSDPLYML TGVIAATEKA LRRAGLALRD IDLFEVNEAF APVVLAWAKD TGADLAKTNV NGGAIAIGHP LGASGARIMT TLVNASEQRG GRYGLQTMCE GGGMANATII ERLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcccagagt cgcgacgccg tcatcgtcgg cgccgtccgc acccccatcg gcaagggcaa ggccaacggc gcactgcacc aggtactacc cgccgacctg ctggcgcata gcctgcgcga actggcggcc cgaaccggcg tcgacccggt acaagtcgac gatgtgatcg ccggcgcggt gacccaggtc ggcgatcagg cggtcaacat cgcacgcaat gcgctgctgg gtgccggatt cccggaatcg gttcccgggg tcaccatcga ccggcaatgc ggcagcagcc agcaggccat cagctttgcc gcccagggcg tgctggccgg tgcttacgat ctggtgatcg ccggcggcgt tgaatcgatg agccgcgtcc cgatgggcac ctcggtgctg cccggaagca acccgttcgg cccggacatg aacaaccgct accccgacgg gctggtcccc cagggcatca gcgccgagct gattgcggca cggtggggct tgtcgcgcac ccaactcgac gagttctccg cagccagcca tgacaaggcc gcccgcgcca ccaaggacgg actcttcgac aacgagctga tcccgatcgc cgggctcacc cacgacgaga tcatccgccc cggcgccacc gtcgatacgc tcgccgcgct gcggccggcg ttctacagcg atgcgatcaa agagcgcttc ccgcagatca actggtcgat caccccgggt aactcgtcac cgttgtccga cagcagcgcc gcggtcatga tcaccagcag cgacgtcgcc cggaagctgg gcctgcgccc gttggcgcgc attcacacca ccacggtggt gggttcggat ccgctctaca tgctgaccgg ggtcatcgcg gccaccgaaa aggcgctacg ccgcgccggg ttggccctgc gcgacatcga cctgttcgag gtcaacgagg ccttcgcccc ggtcgtgctg gcctgggcga aggacaccgg ggcggatctg gccaagacca acgtcaacgg cggcgcaatc gccatcggcc acccgttggg cgccagcggt gcgcgcatca tgaccacgct ggtcaacgct tcggagcagc gcggtggccg ctacggcctg cagaccatgt gcgagggcgg cggcatggcc aacgcgacca tcatcgagcg actggggaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa