MypaA.00654.a

FadA6_2

CENTER ID: MypaA.00654.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00654.a.A1.GE29471 Full length( MypaA.00654.a ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.763
RefSeq: NP_959658.1
UniProt: Q742W1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRETVIVEAV RTPVGKRNGA LSGMHAADLS AIVLNELVER TGIGPELIDD VVWGCVSQVG DQSSNIGRYA VLAAGWPESI PGTTVNRACG SSQQALDFAV QAVMSGQQDV VVAGGVEVMS RVPLGAARAT GMPYGPKVLA RYDDFSFNQG LSAEMIAKKW GFTRTQLDEY SVRSHERAAA AQDAGAFTDQ IVPVFVEDAE NKVVAADEGV RRGSSVEKLA ALKPAFAEDG VIHAGNSSQI SDGAAALLVT TAEMAVELGL TPIVRYVAGA VTGADPVLML TGPIPATEKV LKKAGVALSD VGVFEVNEAF APVPLAWLAE TGADAARTNP LGGAIALGHP LGASGAVLMT RMAHHMRDNG IRYGLQTMCE GGGTANATLV ELVS
NT Sequence
ATGCGTGAAA CAGTCATCGT CGAGGCCGTG CGCACCCCGG TGGGTAAACG CAACGGCGCG CTGTCCGGCA TGCACGCCGC CGACCTGTCC GCGATCGTGC TCAACGAACT CGTCGAACGC ACGGGCATCG GACCCGAGCT CATCGACGAC GTGGTCTGGG GCTGCGTCTC CCAGGTGGGC GACCAGTCCA GCAACATCGG CCGCTACGCG GTGCTGGCCG CGGGCTGGCC GGAAAGCATT CCCGGGACCA CCGTCAACCG GGCCTGCGGC TCCAGCCAGC AGGCGCTGGA CTTCGCCGTG CAGGCGGTGA TGTCGGGTCA GCAGGACGTC GTGGTGGCCG GCGGGGTGGA GGTGATGAGC CGCGTCCCGC TCGGTGCGGC CCGGGCGACC GGCATGCCGT ACGGCCCCAA AGTGCTTGCC CGCTATGACG ACTTCTCCTT CAACCAGGGC CTGTCGGCGG AGATGATCGC CAAGAAGTGG GGGTTCACGC GCACCCAGCT CGACGAGTAC TCAGTCCGCT CGCACGAGCG GGCGGCCGCC GCCCAGGACG CCGGCGCCTT CACCGACCAG ATCGTCCCGG TGTTCGTCGA GGACGCCGAG AACAAGGTCG TCGCCGCCGA CGAGGGCGTG CGGCGGGGAA GCAGCGTGGA GAAGCTGGCC GCGCTCAAAC CGGCCTTCGC CGAGGACGGC GTGATCCATG CCGGGAATTC CTCGCAGATC TCCGACGGTG CGGCCGCGCT GCTGGTGACC ACCGCCGAGA TGGCCGTCGA GCTGGGACTG ACGCCGATCG TGCGGTATGT GGCCGGCGCG GTCACCGGCG CCGACCCGGT GCTGATGCTC ACCGGCCCGA TCCCGGCGAC CGAGAAGGTG TTGAAGAAGG CCGGCGTCGC GCTGTCGGAT GTGGGCGTGT TCGAGGTCAA CGAGGCGTTC GCGCCGGTGC CGCTGGCCTG GCTGGCCGAG ACCGGGGCCG ATGCGGCCCG GACGAATCCG CTGGGCGGCG CCATCGCGCT GGGGCACCCG CTGGGCGCGT CCGGGGCCGT GCTGATGACG CGCATGGCAC ATCACATGCG CGACAACGGA ATTCGTTACG GGCTGCAGAC CATGTGCGAG GGCGGGGGCA CCGCCAACGC CACCCTGGTC GAGCTGGTGA GC
Details for MypaA.00654.a.A1.GE29471
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRETVIVEA VRTPVGKRNG ALSGMHAADL SAIVLNELVE RTGIGPELID DVVWGCVSQV GDQSSNIGRY AVLAAGWPES IPGTTVNRAC GSSQQALDFA VQAVMSGQQD VVVAGGVEVM SRVPLGAARA TGMPYGPKVL ARYDDFSFNQ GLSAEMIAKK WGFTRTQLDE YSVRSHERAA AAQDAGAFTD QIVPVFVEDA ENKVVAADEG VRRGSSVEKL AALKPXFAED GVIHAGNSSQ ISDGAAALLV TTAEMAVXLG LTPIVRYVAG AVTGADPVLM LTXPIPAXXX XXXRPASRXX MWAXSXXTRX LXXXLAWXXX XXXXXXXNXX XXXSXXXPXX XX
Validated NT Sequence
ttttgtttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtg aaacagtcat cgtcgaggcc gtgcgcaccc cggtgggtaa acgcaacggc gcgctgtccg gcatgcacgc cgccgacctg tccgcgatcg tgctcaacga actcgtcgaa cgcacgggca tcggacccga gctcatcgac gacgtggtct ggggctgcgt ctcccaggtg ggcgaccagt ccagcaacat cggccgctac gcggtgctgg ccgcgggctg gccggaaagc attcccggga ccaccgtcaa ccgggcctgc ggctccagcc agcaggcgct ggacttcgcc gtgcaggcgg tgatgtcggg tcagcaggac gtcgtggtgg ccggcggggt ggaggtgatg agccgcgtcc cgctcggtgc ggcccgggcg accggcatgc cgtacggccc caaagtgctt gcccgctatg acgacttctc cttcaaccag ggcctgtcgg cggagatgat cgccaagaag tgggggttca cgcgcaccca gctcgacgag tactcagtcc gctcgcacga gcgggcggcc gccgcccagg acgccggcgc cttcaccgac cagatcgtcc cggtgttcgt cgaggacgcc gagaacaagg tcgtcgccgc cgacgagggc gtgcggcggg gaagcagcgt ggagaagctg gccgcgctca aaccggnctt cgccgaggac ggcgtgatcc atgccgggaa ttcctcgcag atctccgacg gtgcggccgc gctgctggtg accaccgccg agatggccgt cnagctggga ctgacgccga tcgtgcggta tgtggccggc gcggtcaccg gcgccgaccc ggtgctgatg ctcaccngnc cgatcccggc gnncganaan ngtngaanaa ggccggcgtc ncgctgncnn atgtgggcgt gntcnangnc aacnaggcnn ttgcnnntnc nnctggcctg gntggnnnan nnngnnnang nnnnccnnan naatcnnngg nnggngncnt cnngctngnn nccnnnnngn nnnntntgan nannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggntnnn nnnnnnnnnn nngnnncnnn anncnnccnn gnnnnnnnnn nna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRETVIVEA VRTPVGKRNG ALSGMHAADL SAIVLNELVE RTGIGPELID DVVWGCVSQV GDQSSNIGRY AVLAAGWPES IPGTTVNRAC GSSQQALDFA VQAVMSGQQD VVVAGGVEVM SRVPLGAARA TGMPYGPKVL ARYDDFSFNQ GLSAEMIAKK WGFTRTQLDE YSVRSHERAA AAQDAGAFTD QIVPVFVEDA ENKVVAADEG VRRGSSVEKL AALKPAFAED GVIHAGNSSQ ISDGAAALLV TTAEMAVELG LTPIVRYVAG AVTGADPVLM LTGPIPATEK VLKKAGVALS DVGVFEVNEA FAPVPLAWLA ETGADAARTN PLGGAIALGH PLGASGAVLM TRMAHHMRDN GIRYGLQTMC EGGGTANATL VELVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgaaaca gtcatcgtcg aggccgtgcg caccccggtg ggtaaacgca acggcgcgct gtccggcatg cacgccgccg acctgtccgc gatcgtgctc aacgaactcg tcgaacgcac gggcatcgga cccgagctca tcgacgacgt ggtctggggc tgcgtctccc aggtgggcga ccagtccagc aacatcggcc gctacgcggt gctggccgcg ggctggccgg aaagcattcc cgggaccacc gtcaaccggg cctgcggctc cagccagcag gcgctggact tcgccgtgca ggcggtgatg tcgggtcagc aggacgtcgt ggtggccggc ggggtggagg tgatgagccg cgtcccgctc ggtgcggccc gggcgaccgg catgccgtac ggccccaaag tgcttgcccg ctatgacgac ttctccttca accagggcct gtcggcggag atgatcgcca agaagtgggg gttcacgcgc acccagctcg acgagtactc agtccgctcg cacgagcggg cggccgccgc ccaggacgcc ggcgccttca ccgaccagat cgtcccggtg ttcgtcgagg acgccgagaa caaggtcgtc gccgccgacg agggcgtgcg gcggggaagc agcgtggaga agctggccgc gctcaaaccg gccttcgccg aggacggcgt gatccatgcc gggaattcct cgcagatctc cgacggtgcg gccgcgctgc tggtgaccac cgccgagatg gccgtcgagc tgggactgac gccgatcgtg cggtatgtgg ccggcgcggt caccggcgcc gacccggtgc tgatgctcac cggcccgatc ccggcgaccg agaaggtgtt gaagaaggcc ggcgtcgcgc tgtcggatgt gggcgtgttc gaggtcaacg aggcgttcgc gccggtgccg ctggcctggc tggccgagac cggggccgat gcggcccgga cgaatccgct gggcggcgcc atcgcgctgg ggcacccgct gggcgcgtcc ggggccgtgc tgatgacgcg catggcacat cacatgcgcg acaacggaat tcgttacggg ctgcagacca tgtgcgaggg cgggggcacc gccaacgcca ccctggtcga gctggtgagc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa