MyabA.00654.a

Putative acyl-CoA thiolase

CENTER ID: MyabA.00654.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00654.a.A1.GE30326 Full length( MyabA.00654.a ) 1 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3523
RefSeq: YP_001704185.1
UniProt: B1MES5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDAVIIDAV RTPVGKGKPG GSLSGVHPVD LHAHAIRELV ERAGIDPALI DDVISGAVGQ VGEQSSNTAR WAALAAGLPE TVPAVTVDRQ CGSSQQAIHF AAQGVIAGAY DVVIASGIES MSRVPMGSQS LGKDFFGSEV ARRYPEGLVP QGISAELIAA KWNLSREQLD TFAAESHRRA AQAWSEGRFA RDVVPIKAPD VNGDLVEVST DESVRPSTTV EVLAGLKPAF RNELWQQRFP GLDWKVTAGN SSPINDGASA VLITSSETAH RLGLTPRARV HSVAVVGDDP LYMLTGIIPA TAKVLDRAGL SLADIDAFEV NEAFASVVLA WQAETGADLS KVNVNGGATA IGHPLGASGG RLMTTLVSVL EQGGGRYGLQ TMCEAGGLAN ATILERL
NT Sequence
ATGAGGGATG CGGTGATCAT CGACGCGGTG CGGACACCGG TCGGCAAGGG CAAGCCAGGG GGGTCGCTCT CGGGTGTGCA TCCGGTTGAT CTGCACGCGC ATGCGATTCG TGAACTCGTT GAACGCGCCG GTATTGATCC GGCACTAATC GACGACGTGA TCAGCGGCGC GGTGGGGCAG GTGGGTGAGC AGAGCTCCAA TACGGCGCGC TGGGCGGCGC TGGCCGCGGG ACTGCCCGAG ACGGTTCCGG CGGTGACCGT CGACCGTCAA TGTGGCAGTA GTCAGCAGGC CATCCACTTT GCCGCGCAAG GTGTAATCGC AGGAGCCTAT GACGTCGTGA TCGCCTCGGG TATCGAATCG ATGAGCCGAG TGCCCATGGG AAGTCAAAGT TTGGGTAAGG ACTTCTTCGG TTCCGAAGTA GCGCGACGAT ACCCGGAAGG GCTTGTCCCG CAGGGCATCA GCGCCGAGCT GATCGCCGCG AAATGGAACC TGTCACGGGA GCAGCTGGAT ACGTTTGCCG CAGAGAGTCA CCGTCGTGCT GCACAAGCGT GGTCGGAGGG CAGATTTGCC CGCGATGTGG TGCCCATCAA GGCCCCCGAC GTCAACGGTG ACCTGGTCGA GGTGTCCACC GACGAGTCCG TGCGGCCGTC GACCACGGTT GAGGTGCTCG CCGGACTCAA GCCGGCCTTC CGTAATGAAC TCTGGCAACA GCGCTTCCCG GGGCTTGATT GGAAGGTGAC CGCGGGCAAC TCGTCGCCGA TCAACGATGG CGCCTCTGCT GTGCTCATCA CATCCAGTGA GACCGCTCAT CGTCTCGGCC TGACGCCGCG CGCTCGCGTG CATTCGGTGG CGGTGGTGGG CGACGATCCG TTATACATGC TGACCGGCAT CATCCCGGCA ACCGCGAAAG TACTCGATAG AGCAGGGTTA TCGTTGGCGG ACATCGATGC CTTCGAGGTC AACGAGGCTT TCGCGTCGGT GGTGCTCGCT TGGCAGGCCG AGACCGGTGC GGACCTGTCC AAGGTAAACG TCAACGGGGG AGCCACCGCG ATCGGCCACC CGCTCGGCGC CAGTGGCGGA CGGCTGATGA CTACGCTGGT ATCGGTGCTG GAACAGGGCG GTGGACGGTA CGGCCTGCAA ACGATGTGTG AAGCAGGCGG TTTGGCTAAT GCCACCATCC TTGAGCGGCT T
Details for MyabA.00654.a.A1.GE30326
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRDAVIIDA VRTPVGKGKP GGSLSGVHPV DLHAHAIREL VERAGIDPAL IDDVISGAVG QVGEQSSNTA RWAALAAGLP ETVPAVTVDR QCGSSQQAIH FAAQGVIAGA YDVVIASGIE SMSRVPMGSQ SLGKDFFGSE VARRYPEGLV PQGISAELIA AKWNLSREQL DTFAAXSHRR AAQAWSEGRF ARDVVPIKAP DVNGDLVEVS TDESVRPSTT VXVLAGXXPA FRNELWQQRF PGLDWXVTAG NSSPINDGAS AVLITSSETA HRLGLTPRAR VHSVAVXGDD PXYMLTGIXP ATAKXLDXXX XXXRTSXPXX XTXXXXVXXL XXXXXXXXXX XXXXXGXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
annnttttgt ttactttaag aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga gggatgcggt gatcatcgac gcggtgcgga caccggtcgg caagggcaag ccaggggggt cgctctcggg tgtgcatccg gttgatctgc acgcgcatgc gattcgtgaa ctcgttgaac gcgccggtat tgatccggca ctaatcgacg acgtgatcag cggcgcggtg gggcaggtgg gtgagcagag ctccaatacg gcgcgctggg cggcgctggc cgcgggactg cccgagacgg ttccggcggt gaccgtcgac cgtcaatgtg gcagtagtca gcaggccatc cactttgccg cgcaaggtgt aatcgcagga gcctatgacg tcgtgatcgc ctcgggtatc gaatcgatga gccgagtgcc catgggaagt caaagtttgg gtaaggactt cttcggttcc gaagtagcgc gacgataccc ggaagggctt gtcccgcagg gcatcagcgc cgagctgatc gccgcgaaat ggaacctgtc acgggagcag ctggatacgt ttgccgcana gagtcaccgt cgtgctgcac aagcgtggtc ggagggcaga tttgcccgcg atgtggtgcc catcaaggcc cccgacgtca acggtgacct ggtcgaggtg tccaccgacg agtccgtgcg gccgtcgacc acggttgang tgctcgccgg antcnagccg gccttccgta atgaactctg gcaacagcgc ttcccggggc ttgattggaa ngtgaccgcg ggcaactcgt cgccgatcaa cgatggcgcc tctgctgtgc tcatcacatc nagtgagacc gctcatcgtc tcggcctgac gccgcgcgct cgcgtgcatt cngtggcngt ggngggcgac gatccnnnnt acatgctgac cggcatcntc ccggcaaccg cnaaantact cgatananca gnnnatcnnn ggcggacatc nannccnncn angncaacga nnnctttnnn cgtnnnnnnn ctcncnngnn nnngannnng nnncnnnnnn nnnnnnnaan ntnnncnggg ggnnncnnnn tnnnnnncnn cntnggnnnc cnnnnnnnnn nngnnnnnna nnnnnnnnna nnnnnnnnnn nnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRDAVIIDA VRTPVGKGKP GGSLSGVHPV DLHAHAIREL VERAGIDPAL IDDVISGAVG QVGEQSSNTA RWAALAAGLP ETVPAVTVDR QCGSSQQAIH FAAQGVIAGA YDVVIASGIE SMSRVPMGSQ SLGKDFFGSE VARRYPEGLV PQGISAELIA AKWNLSREQL DTFAAESHRR AAQAWSEGRF ARDVVPIKAP DVNGDLVEVS TDESVRPSTT VEVLAGLKPA FRNELWQQRF PGLDWKVTAG NSSPINDGAS AVLITSSETA HRLGLTPRAR VHSVAVVGDD PLYMLTGIIP ATAKVLDRAG LSLADIDAFE VNEAFASVVL AWQAETGADL SKVNVNGGAT AIGHPLGASG GRLMTTLVSV LEQGGGRYGL QTMCEAGGLA NATILERL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagggatgcg gtgatcatcg acgcggtgcg gacaccggtc ggcaagggca agccaggggg gtcgctctcg ggtgtgcatc cggttgatct gcacgcgcat gcgattcgtg aactcgttga acgcgccggt attgatccgg cactaatcga cgacgtgatc agcggcgcgg tggggcaggt gggtgagcag agctccaata cggcgcgctg ggcggcgctg gccgcgggac tgcccgagac ggttccggcg gtgaccgtcg accgtcaatg tggcagtagt cagcaggcca tccactttgc cgcgcaaggt gtaatcgcag gagcctatga cgtcgtgatc gcctcgggta tcgaatcgat gagccgagtg cccatgggaa gtcaaagttt gggtaaggac ttcttcggtt ccgaagtagc gcgacgatac ccggaagggc ttgtcccgca gggcatcagc gccgagctga tcgccgcgaa atggaacctg tcacgggagc agctggatac gtttgccgca gagagtcacc gtcgtgctgc acaagcgtgg tcggagggca gatttgcccg cgatgtggtg cccatcaagg cccccgacgt caacggtgac ctggtcgagg tgtccaccga cgagtccgtg cggccgtcga ccacggttga ggtgctcgcc ggactcaagc cggccttccg taatgaactc tggcaacagc gcttcccggg gcttgattgg aaggtgaccg cgggcaactc gtcgccgatc aacgatggcg cctctgctgt gctcatcaca tccagtgaga ccgctcatcg tctcggcctg acgccgcgcg ctcgcgtgca ttcggtggcg gtggtgggcg acgatccgtt atacatgctg accggcatca tcccggcaac cgcgaaagta ctcgatagag cagggttatc gttggcggac atcgatgcct tcgaggtcaa cgaggctttc gcgtcggtgg tgctcgcttg gcaggccgag accggtgcgg acctgtccaa ggtaaacgtc aacgggggag ccaccgcgat cggccacccg ctcggcgcca gtggcggacg gctgatgact acgctggtat cggtgctgga acagggcggt ggacggtacg gcctgcaaac gatgtgtgaa gcaggcggtt tggctaatgc caccatcctt gagcggcttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa