MysmA.00247.b
Putative acyl-CoA dehydrogenase
| CENTER ID: | MysmA.00247.b |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00247.b.A1.GE28587 | Full length( MysmA.00247.b ) | 1 | 382 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00247.b.A1.PW28486 | Full length( MysmA.00247.b ) | 1 | 382 |
Structures
3OIB
DEPOSITED: 8/18/2010
DETERMINATION: XRay
CLONE: MysmA.00247.b.A1.GE28587
PROTEIN: MysmA.00247.b.A1.PW28486
3P4T
DEPOSITED: 10/7/2010
DETERMINATION: XRay
CLONE: MysmA.00247.b.A1.GE28587
PROTEIN: MysmA.00247.b.A1.PW28486
Publications by SSGCID
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PMID: 21359836; PMCID: PMC3123459
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.5351 |
| RefSeq: | YP_889729.1 |
| UniProt: | A0R3J1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIWTTAERE ALRKTVRAFA EREVLPHAHE WERAGEIPRE LHRKAAELGL LGAGFPEDAG GSGGDGADPV VICEEMHYAG SPGGVYASLF TCGIAVPHMI ASGDQRLIDT YVRPTLRGEK IGALAITEPG GGSDVGHLRT RADLDGDHYV INGAKTYITS GVRADYVVTA ARTGGPGAGG VSLIVVDKGT PGFEVTRKLD KMGWRSSDTA ELSYTDVRVP VANLVGSENT GFAQIAAAFV AERVGLATQA YAGAQRCLDL TVEWCRNRDT FGRPLISRQA VQNTLAGMAR RIDVARVYTR HVVERQLAGE TNLIAEVCFA KNTAVEAGEW VANQAVQLFG GMGYMAESEV ERQYRDMRIL GIGGGTTEIL TSLAAKTLGF QS
NT Sequence
ATGAGCATCT GGACCACCGC AGAACGCGAG GCACTGCGAA AAACCGTGCG CGCCTTCGCC GAACGCGAGG TGCTTCCGCA CGCCCACGAA TGGGAACGCG CCGGGGAGAT CCCCCGCGAA CTGCACCGCA AGGCCGCGGA ACTGGGTCTG CTGGGCGCGG GCTTCCCCGA GGATGCGGGC GGCAGCGGCG GGGACGGCGC CGATCCGGTG GTCATCTGCG AGGAGATGCA CTACGCGGGC TCACCCGGCG GGGTGTACGC ATCGCTGTTC ACGTGCGGTA TCGCTGTCCC CCACATGATC GCCTCGGGCG ACCAGCGGCT CATCGACACC TATGTGCGGC CGACACTGCG CGGCGAGAAG ATCGGCGCGC TGGCCATCAC CGAACCCGGC GGCGGATCCG ACGTCGGGCA TCTGCGCACC CGCGCCGATC TGGACGGCGA CCACTACGTC ATCAACGGCG CCAAGACCTA CATCACCTCG GGTGTGCGCG CCGACTACGT CGTGACCGCC GCACGCACAG GCGGCCCGGG CGCGGGCGGC GTCTCGCTGA TCGTGGTGGA CAAGGGCACA CCGGGATTCG AGGTGACCCG CAAGCTCGAC AAGATGGGTT GGCGCTCAAG CGATACCGCC GAGCTGTCCT ACACCGATGT GCGGGTGCCC GTGGCGAACC TGGTGGGCAG CGAGAACACG GGGTTCGCGC AGATCGCGGC CGCGTTCGTG GCCGAACGGG TGGGCCTGGC CACGCAGGCC TACGCGGGCG CGCAGCGCTG CCTGGACCTC ACCGTCGAGT GGTGCCGCAA CCGGGACACG TTCGGGCGTC CACTGATCTC ACGGCAGGCG GTGCAGAACA CACTCGCGGG CATGGCCCGG CGCATCGACG TCGCGCGGGT GTACACGCGG CACGTGGTGG AACGACAACT CGCGGGCGAG ACGAATCTGA TCGCCGAGGT GTGCTTCGCC AAGAACACCG CCGTGGAGGC CGGCGAGTGG GTGGCCAACC AGGCCGTCCA ATTGTTCGGC GGCATGGGCT ACATGGCCGA GTCCGAGGTC GAGCGCCAGT ACCGCGACAT GCGCATCCTC GGCATCGGCG GCGGCACCAC CGAGATCTTG ACCAGCCTGG CCGCCAAGAC GCTGGGGTTC CAGTCA
Details for MysmA.00247.b.A1.GE28587
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MSIWTTAERE ALRKTVRAFA EREVLPHAHE WERAGEIPRE LHRKAAELGL LGAGFPEDAG GSGGDGADPV VICEEMHYAG SPGGVYASLF TCGIAVPHMI ASGDQRLIDT YVRPTLRGEK IGALAITEPG GGSDVGHLRT RADLDGDHYV INGAKTYITS GVRADYVVTA ARTGGPGAGG VSLIVVDKGT PGFEVTRKLD KMGWRSSDTA ELSYTDVRVP VANLVGSENT GFAQIAAAFV AERVGLATQA YAGAQRCLDL TVEWCRNRDT FGRPLISRQA VQNTLAGMAR RIDVARVYTR HVVERQLAGE TNLIAEVCFA KNTAVEAGEW VANQAVQLFG GMGYMAESEV ERQYRDMRIL GIGGGTTEIL TS
Validated NT Sequence
aggctggtca agatctcggt ggtgccgccg ccgatgccga ggatgcgcat gtcgcggtac tggcgctcga cctcggactc ggccatgtag cccatgccgc cgaacaattg gacggcctgg ttggccaccc actcgccggc ctccacggcg gtgttcttgg cgaagcacac ctcggcgatc agattcgtct cgcccgcgag ttgtcgttcc accacgtgcc gcgtgtacac ccgcgcgacg tcgatgcgcc gggccatgcc cgcgagtgtg ttctgcaccg cctgccgtga gatcagtgga cgcccgaacg tgtcccggtt gcggcaccac tcgacggtga ggtccaggca gcgctgcgcg cccgcgtagg cctgcgtggc caggcccacc cgttcggcca cgaacgcggc cgcgatctgc gcgaaccccg tgttctcgct gcccaccagg ttcgccacgg gcacccgcac atcggtgtag gacagctcgg cggtatcgct tgagcgccaa cccatcttgt cgagcttgcg ggtcacctcg aatcccggtg tgcccttgtc caccacgatc agcgagacgc cgcccgcgcc cgggccgcct gtgcgtgcgg cggtcacgac gtagtcggcg cgcacacccg aggtgatgta ggtcttggcg ccgttgatga cgtagtggtc gccgtccaga tcggcgcggg tgcgcagatg cccgacgtcg gatccgccgc cgggttcggt gatggccagc gcgccgatct tctcgccgcg cagtgtcggc cgcacatagg tgtcgatgag ccgctggtcg cccgaggcga tcatgtgggg gacagcgata ccgcacgtga acagcgatgc gtacaccccg ccgggtgagc ccgcgtagtg catctcctcg cagatgacca ccggatcggc gccgtccccg ccgctgccgc ccgcatcctc ggggaagccc gcgcccagca gacccagttc cgcggccttg cggtgcagtt cgcgggggat ctccccggcg cgttcccatt cgtgggcgtg cggaagcacc tcgcgttcgg cgaaggcgcg cacggttttt cgcagtgcct cgcgttctgc ggtggtccag atgctcatcg a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIWTTAER EALRKTVRAF AEREVLPHAH EWERAGEIPR ELHRKAAELG LLGAGFPEDA GGSGGDGADP VVICEEMHYA GSPGGVYASL FTCGIAVPHM IASGDQRLID TYVRPTLRGE KIGALAITEP GGGSDVGHLR TRADLDGDHY VINGAKTYIT SGVRADYVVT AARTGGPGAG GVSLIVVDKG TPGFEVTRKL DKMGWRSSDT AELSYTDVRV PVANLVGSEN TGFAQIAAAF VAERVGLATQ AYAGAQRCLD LTVEWCRNRD TFGRPLISRQ AVQNTLAGMA RRIDVARVYT RHVVERQLAG ETNLIAEVCF AKNTAVEAGE WVANQAVQLF GGMGYMAESE VERQYRDMRI LGIGGGTTEI LTSLAAKTLG FQS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcatctgg accaccgcag aacgcgaggc actgcgaaaa accgtgcgcg ccttcgccga acgcgaggtg cttccgcacg cccacgaatg ggaacgcgcc ggggagatcc cccgcgaact gcaccgcaag gccgcggaac tgggtctgct gggcgcgggc ttccccgagg atgcgggcgg cagcggcggg gacggcgccg atccggtggt catctgcgag gagatgcact acgcgggctc acccggcggg gtgtacgcat cgctgttcac gtgcggtatc gctgtccccc acatgatcgc ctcgggcgac cagcggctca tcgacaccta tgtgcggccg acactgcgcg gcgagaagat cggcgcgctg gccatcaccg aacccggcgg cggatccgac gtcgggcatc tgcgcacccg cgccgatctg gacggcgacc actacgtcat caacggcgcc aagacctaca tcacctcggg tgtgcgcgcc gactacgtcg tgaccgccgc acgcacaggc ggcccgggcg cgggcggcgt ctcgctgatc gtggtggaca agggcacacc gggattcgag gtgacccgca agctcgacaa gatgggttgg cgctcaagcg ataccgccga gctgtcctac accgatgtgc gggtgcccgt ggcgaacctg gtgggcagcg agaacacggg gttcgcgcag atcgcggccg cgttcgtggc cgaacgggtg ggcctggcca cgcaggccta cgcgggcgcg cagcgctgcc tggacctcac cgtcgagtgg tgccgcaacc gggacacgtt cgggcgtcca ctgatctcac ggcaggcggt gcagaacaca ctcgcgggca tggcccggcg catcgacgtc gcgcgggtgt acacgcggca cgtggtggaa cgacaactcg cgggcgagac gaatctgatc gccgaggtgt gcttcgccaa gaacaccgcc gtggaggccg gcgagtgggt ggccaaccag gccgtccaat tgttcggcgg catgggctac atggccgagt ccgaggtcga gcgccagtac cgcgacatgc gcatcctcgg catcggcggc ggcaccaccg agatcttgac cagcctggcc gccaagacgc tggggttcca gtcaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.00247.b.A1.PW28486
| PURIFICATION DATe: | 4/12/2010 |
| CONCENTRATION: | 26.75mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSIWTTAERE ALRKTVRAFA EREVLPHAHE WERAGEIPRE LHRKAAELGL LGAGFPEDAG GSGGDGADPV VICEEMHYAG SPGGVYASLF TCGIAVPHMI ASGDQRLIDT YVRPTLRGEK IGALAITEPG GGSDVGHLRT RADLDGDHYV INGAKTYITS GVRADYVVTA ARTGGPGAGG VSLIVVDKGT PGFEVTRKLD KMGWRSSDTA ELSYTDVRVP VANLVGSENT GFAQIAAAFV AERVGLATQA YAGAQRCLDL TVEWCRNRDT FGRPLISRQA VQNTLAGMAR RIDVARVYTR HVVERQLAGE TNLIAEVCFA KNTAVEAGEW VANQAVQLFG GMGYMAESEV ERQYRDMRIL GIGGGTTEIL TS
Validated NT Sequence
aggctggtca agatctcggt ggtgccgccg ccgatgccga ggatgcgcat gtcgcggtac tggcgctcga cctcggactc ggccatgtag cccatgccgc cgaacaattg gacggcctgg ttggccaccc actcgccggc ctccacggcg gtgttcttgg cgaagcacac ctcggcgatc agattcgtct cgcccgcgag ttgtcgttcc accacgtgcc gcgtgtacac ccgcgcgacg tcgatgcgcc gggccatgcc cgcgagtgtg ttctgcaccg cctgccgtga gatcagtgga cgcccgaacg tgtcccggtt gcggcaccac tcgacggtga ggtccaggca gcgctgcgcg cccgcgtagg cctgcgtggc caggcccacc cgttcggcca cgaacgcggc cgcgatctgc gcgaaccccg tgttctcgct gcccaccagg ttcgccacgg gcacccgcac atcggtgtag gacagctcgg cggtatcgct tgagcgccaa cccatcttgt cgagcttgcg ggtcacctcg aatcccggtg tgcccttgtc caccacgatc agcgagacgc cgcccgcgcc cgggccgcct gtgcgtgcgg cggtcacgac gtagtcggcg cgcacacccg aggtgatgta ggtcttggcg ccgttgatga cgtagtggtc gccgtccaga tcggcgcggg tgcgcagatg cccgacgtcg gatccgccgc cgggttcggt gatggccagc gcgccgatct tctcgccgcg cagtgtcggc cgcacatagg tgtcgatgag ccgctggtcg cccgaggcga tcatgtgggg gacagcgata ccgcacgtga acagcgatgc gtacaccccg ccgggtgagc ccgcgtagtg catctcctcg cagatgacca ccggatcggc gccgtccccg ccgctgccgc ccgcatcctc ggggaagccc gcgcccagca gacccagttc cgcggccttg cggtgcagtt cgcgggggat ctccccggcg cgttcccatt cgtgggcgtg cggaagcacc tcgcgttcgg cgaaggcgcg cacggttttt cgcagtgcct cgcgttctgc ggtggtccag atgctcatcg a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIWTTAER EALRKTVRAF AEREVLPHAH EWERAGEIPR ELHRKAAELG LLGAGFPEDA GGSGGDGADP VVICEEMHYA GSPGGVYASL FTCGIAVPHM IASGDQRLID TYVRPTLRGE KIGALAITEP GGGSDVGHLR TRADLDGDHY VINGAKTYIT SGVRADYVVT AARTGGPGAG GVSLIVVDKG TPGFEVTRKL DKMGWRSSDT AELSYTDVRV PVANLVGSEN TGFAQIAAAF VAERVGLATQ AYAGAQRCLD LTVEWCRNRD TFGRPLISRQ AVQNTLAGMA RRIDVARVYT RHVVERQLAG ETNLIAEVCF AKNTAVEAGE WVANQAVQLF GGMGYMAESE VERQYRDMRI LGIGGGTTEI LTSLAAKTLG FQS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCATCTGG ACCACCGCAG AACGCGAGGC ACTGCGAAAA ACCGTGCGCG CCTTCGCCGA ACGCGAGGTG CTTCCGCACG CCCACGAATG GGAACGCGCC GGGGAGATCC CCCGCGAACT GCACCGCAAG GCCGCGGAAC TGGGTCTGCT GGGCGCGGGC TTCCCCGAGG ATGCGGGCGG CAGCGGCGGG GACGGCGCCG ATCCGGTGGT CATCTGCGAG GAGATGCACT ACGCGGGCTC ACCCGGCGGG GTGTACGCAT CGCTGTTCAC GTGCGGTATC GCTGTCCCCC ACATGATCGC CTCGGGCGAC CAGCGGCTCA TCGACACCTA TGTGCGGCCG ACACTGCGCG GCGAGAAGAT CGGCGCGCTG GCCATCACCG AACCCGGCGG CGGATCCGAC GTCGGGCATC TGCGCACCCG CGCCGATCTG GACGGCGACC ACTACGTCAT CAACGGCGCC AAGACCTACA TCACCTCGGG TGTGCGCGCC GACTACGTCG TGACCGCCGC ACGCACAGGC GGCCCGGGCG CGGGCGGCGT CTCGCTGATC GTGGTGGACA AGGGCACACC GGGATTCGAG GTGACCCGCA AGCTCGACAA GATGGGTTGG CGCTCAAGCG ATACCGCCGA GCTGTCCTAC ACCGATGTGC GGGTGCCCGT GGCGAACCTG GTGGGCAGCG AGAACACGGG GTTCGCGCAG ATCGCGGCCG CGTTCGTGGC CGAACGGGTG GGCCTGGCCA CGCAGGCCTA CGCGGGCGCG CAGCGCTGCC TGGACCTCAC CGTCGAGTGG TGCCGCAACC GGGACACGTT CGGGCGTCCA CTGATCTCAC GGCAGGCGGT GCAGAACACA CTCGCGGGCA TGGCCCGGCG CATCGACGTC GCGCGGGTGT ACACGCGGCA CGTGGTGGAA CGACAACTCG CGGGCGAGAC GAATCTGATC GCCGAGGTGT GCTTCGCCAA GAACACCGCC GTGGAGGCCG GCGAGTGGGT GGCCAACCAG GCCGTCCAAT TGTTCGGCGG CATGGGCTAC ATGGCCGAGT CCGAGGTCGA GCGCCAGTAC CGCGACATGC GCATCCTCGG CATCGGCGGC GGCACCACCG AGATCTTGAC CAGCCTGGCC GCCAAGACGC TGGGGTTCCA GTCAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA