MyboA.00247.a

PROBABLE ACYL-COA DEHYDROGENASE FADE13

CENTER ID: MyboA.00247.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.00247.a.A1.GE30554 Full length( MyboA.00247.a ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.1089
RefSeq: NP_854657.1
UniProt: A0A0H3P2A7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIWTTPERQ QLRKTVRAFA EREILPHVDE WERIGELPRG LHRLAGAAGL LGAGFPEAVG GGGGDGADPV IICEEMHQAG APGGVYASLF TCGIAVPHMV ASGDERLIAT YVRPTLAGEK IGALAITEPG GGSDVGHLRT SAVRDGDHYV INGAKTYITS GVRADYVVTA VRTGGPGAAG VSLLVVEKDT PGFEVTRKLD KMGWRSSDTA ELCYTDVAVP ATNLVGAENS GFTQIARAFV SERIGLAAQA YSSAQRCLDL TAQWCRDRET FGRPLISRQS VQNTLAEMAR RIDVARVYAH HVVERQLAGE TDLIAQVCFA KNTAVQAGEW VANQAVQLFG GMGYMAESEA NANTGTCESS VSEAAPPKY
NT Sequence
GTGAACATCT GGACCACACC GGAGCGCCAA CAGCTGCGAA AAACGGTGCG CGCCTTTGCC GAGCGCGAGA TCCTGCCGCA CGTCGACGAG TGGGAAAGGA TCGGCGAGTT GCCCCGCGGC CTGCACCGGC TCGCCGGGGC CGCCGGGCTG CTGGGCGCCG GCTTTCCCGA GGCGGTCGGT GGTGGCGGCG GTGACGGCGC CGACCCCGTC ATCATCTGCG AGGAGATGCA CCAGGCCGGC GCCCCGGGCG GGGTGTATGC GTCCCTGTTC ACCTGCGGTA TCGCCGTACC GCACATGGTC GCATCCGGCG ACGAACGGCT GATCGCCACG TACGTGCGGC CCACCTTGGC CGGGGAGAAG ATCGGCGCGC TGGCCATCAC CGAACCCGGC GGCGGCTCCG ACGTCGGGCA TCTACGGACC AGCGCGGTAC GGGACGGCGA CCATTACGTG ATCAACGGCG CCAAGACCTA CATCACCTCC GGCGTGCGGG CCGACTACGT GGTCACCGCG GTGCGAACCG GTGGACCCGG TGCCGCCGGG GTTTCGCTGC TGGTGGTGGA AAAGGACACC CCCGGCTTTG AGGTGACCCG CAAGCTGGAC AAGATGGGCT GGCGTTCCTC GGATACCGCC GAGCTGTGCT ACACCGACGT GGCCGTGCCG GCGACTAACC TCGTGGGCGC CGAGAACAGC GGCTTCACCC AGATCGCCCG GGCATTTGTT TCCGAGCGCA TCGGCCTTGC CGCCCAGGCG TATTCGAGCG CCCAGCGGTG CCTGGACCTC ACCGCGCAGT GGTGCCGGGA CCGCGAGACG TTCGGTCGGC CGCTGATTTC ACGCCAGTCG GTGCAGAACA CGCTGGCCGA GATGGCCCGC CGCATCGACG TCGCCCGGGT CTATGCGCAC CATGTGGTGG AACGCCAGCT CGCCGGCGAG ACCGACCTGA TCGCGCAGGT GTGTTTCGCC AAGAACACCG CCGTGCAGGC CGGGGAGTGG GTTGCCAACC AGGCCGTACA GCTGTTCGGC GGCATGGGTT ACATGGCCGA ATCCGAAGCG AACGCCAATA CCGGGACATG CGAATCCTCG GTATCGGAGG CGGCACCACC GAAATAC
Details for MyboA.00247.a.A1.GE30554
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNIWTTPE RQQLRKTVRA FAEREILPHV DEWERIGELP RGLHRLAGAA GLLGAGFPEA VGGGGGDGAD PVIICEEMHQ AGAPGGVYAS LFTCGIAVPH MVASGDERLI ATYVRPTLAG EKIGALAITE PGGGSDVGHL RTSAVRDGDH YVINGAKTYI TSGVRADYVV TAVRTGGPGA AGVSLLVVEK DTPGFEVTRK LDKMGWRSSD TAELCYTDVA VPATNLVGAE NSGFTQIARA FVSERIGLAA XAYSSAQRCL DLTAQWCXDR ETFGRPLISX QSXQNTLAXM ARRIXXARXM RTXXXXXSSP XXPXXAXXCF XXTXPXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga acatctggac cacaccggag cgccaacagc tgcgaaaaac ggtgcgcgcc tttgccgagc gcgagatcct gccgcacgtc gacgagtggg aaaggatcgg cgagttgccc cgcggcctgc accggctcgc cggggccgcc gggctgctgg gcgccggctt tcccgaggcg gtcggtggtg gcggcggtga cggcgccgac cccgtcatca tctgcgagga gatgcaccag gccggcgccc cgggcggggt gtatgcgtcc ctgttcacct gcggtatcgc cgtaccgcac atggtcgcat ccggcgacga acggctgatc gccacgtacg tgcggcccac cttggccggg gagaagatcg gcgcgctggc catcaccgaa cccggcggcg gctccgacgt cgggcatcta cggaccagcg cggtacggga cggcgaccat tacgtgatca acggcgccaa gacctacatc acctccggcg tgcgggccga ctacgtggtc accgcggtgc gaaccggtgg acccggtgcc gccggggttt cgctgctggt ggtggaaaag gacacccccg gctttgaggt gacccgcaag ctggacaaga tgggctggcg ttcctcggat accgccgagc tgtgctacac cgacgtggcc gtgccggcga ctaacctcgt gggcgccgag aacagcggct tcacccagat cgcccgggca tttgtttccg agcgcatcgg ccttgccgcc cangcgtatt cgagcgccca gcggtgcctg gacctcaccg cgcagtggtg ccnggaccgc gagacgttcg gtcggccgct gatttcannc cagtcggngc agaacacgct ggccganatg gcccgncgca tcnnnntcgc ccggnctatg cgcacnntgn ngnggancnn cagctcnccn gnnannccga nnntcgcncn gnngtgtttc nnnnnaacnn cgcccnnncn nnnnnnnnnn nnnnnncanc nnnngnnnnn gnnnnnnnnn nnancnnnnn nnnnnnnnnn ngnnnnnnnn nnnnnnngnn nncnanna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNIWTTPE RQQLRKTVRA FAEREILPHV DEWERIGELP RGLHRLAGAA GLLGAGFPEA VGGGGGDGAD PVIICEEMHQ AGAPGGVYAS LFTCGIAVPH MVASGDERLI ATYVRPTLAG EKIGALAITE PGGGSDVGHL RTSAVRDGDH YVINGAKTYI TSGVRADYVV TAVRTGGPGA AGVSLLVVEK DTPGFEVTRK LDKMGWRSSD TAELCYTDVA VPATNLVGAE NSGFTQIARA FVSERIGLAA QAYSSAQRCL DLTAQWCRDR ETFGRPLISR QSVQNTLAEM ARRIDVARVY AHHVVERQLA GETDLIAQVC FAKNTAVQAG EWVANQAVQL FGGMGYMAES EANANTGTCE SSVSEAAPPK Y
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacatc tggaccacac cggagcgcca acagctgcga aaaacggtgc gcgcctttgc cgagcgcgag atcctgccgc acgtcgacga gtgggaaagg atcggcgagt tgccccgcgg cctgcaccgg ctcgccgggg ccgccgggct gctgggcgcc ggctttcccg aggcggtcgg tggtggcggc ggtgacggcg ccgaccccgt catcatctgc gaggagatgc accaggccgg cgccccgggc ggggtgtatg cgtccctgtt cacctgcggt atcgccgtac cgcacatggt cgcatccggc gacgaacggc tgatcgccac gtacgtgcgg cccaccttgg ccggggagaa gatcggcgcg ctggccatca ccgaacccgg cggcggctcc gacgtcgggc atctacggac cagcgcggta cgggacggcg accattacgt gatcaacggc gccaagacct acatcacctc cggcgtgcgg gccgactacg tggtcaccgc ggtgcgaacc ggtggacccg gtgccgccgg ggtttcgctg ctggtggtgg aaaaggacac ccccggcttt gaggtgaccc gcaagctgga caagatgggc tggcgttcct cggataccgc cgagctgtgc tacaccgacg tggccgtgcc ggcgactaac ctcgtgggcg ccgagaacag cggcttcacc cagatcgccc gggcatttgt ttccgagcgc atcggccttg ccgcccaggc gtattcgagc gcccagcggt gcctggacct caccgcgcag tggtgccggg accgcgagac gttcggtcgg ccgctgattt cacgccagtc ggtgcagaac acgctggccg agatggcccg ccgcatcgac gtcgcccggg tctatgcgca ccatgtggtg gaacgccagc tcgccggcga gaccgacctg atcgcgcagg tgtgtttcgc caagaacacc gccgtgcagg ccggggagtg ggttgccaac caggccgtac agctgttcgg cggcatgggt tacatggccg aatccgaagc gaacgccaat accgggacat gcgaatcctc ggtatcggag gcggcaccac cgaaatacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a