MyabA.00247.c

Probable acyl-CoA dehydrogenase FadE

CENTER ID: MyabA.00247.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00247.c.A1.GE30268 Full length( MyabA.00247.c ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1111
RefSeq: YP_001701817.1
UniProt: B1MJK7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVNSWNTP ERKALRETVR DFAEREILPN VNEWEREGLL PRELHRKAGD LGLLGPGSPE AVGGGGGDAI DPVIVCEELH YAGVPGGVFA SLFTCGISTP HMIASGDQRL IDNYVKPTLA GELIGSLAIT EPGGGSDVGH LTTSAKRDGD HYIVNGAKTF ITSGVRADYV VTAVRTGGPG AAGVSLLLID KDTPGFEVAR KLDKMGWRSS DTAELSFTDV RVPAENLVGS ENTGFAQIAG AFVSERIGLA AQAYASAQRC LDLTVEWCRN RETFGRPLIS RQSVQNTLAE MARRIDVARV YTHALVDRAI AGESNLIAEV CFAKNTAVEA GEWVANQGVQ LFGGMGYMAE SEIERQYRDM RIIGIGGGTT EILTSLAAKL LGFQS
NT Sequence
GTGACCGCCG TGAATTCTTG GAACACGCCC GAACGCAAGG CACTTCGGGA GACCGTGCGT GATTTCGCCG AGCGCGAGAT CCTGCCGAAT GTCAACGAAT GGGAACGTGA GGGGCTGCTC CCGCGCGAAT TGCACCGCAA GGCCGGTGAT CTGGGCCTGC TCGGGCCCGG CTCGCCCGAA GCGGTCGGCG GCGGAGGTGG CGATGCCATC GACCCGGTGA TCGTGTGCGA GGAACTGCAC TACGCGGGCG TGCCCGGTGG CGTGTTCGCC TCCCTGTTCA CCTGTGGGAT CTCCACCCCG CACATGATCG CCTCCGGCGA TCAGCGGCTC ATCGACAATT ATGTGAAGCC AACCCTGGCC GGGGAACTGA TCGGCTCGCT TGCCATCACC GAACCCGGCG GCGGGTCCGA TGTCGGTCAC CTGACCACAT CGGCGAAGCG GGACGGCGAC CACTACATCG TCAACGGCGC CAAGACCTTC ATCACCTCCG GTGTACGCGC CGACTATGTG GTGACCGCGG TCCGCACCGG CGGCCCGGGA GCCGCCGGAG TTTCGCTGCT CCTGATCGAC AAGGACACAC CGGGATTTGA GGTGGCTCGC AAGCTCGACA AGATGGGCTG GCGTTCCTCG GACACCGCCG AGCTGTCCTT CACCGACGTG CGTGTACCCG CCGAAAACCT GGTGGGGTCC GAGAACACCG GTTTCGCACA GATCGCGGGC GCCTTCGTCT CGGAACGCAT CGGTCTTGCC GCGCAGGCGT ATGCGAGCGC GCAGCGCTGC CTGGACCTGA CCGTGGAGTG GTGCCGCAAC CGGGAGACGT TCGGTCGGCC ACTCATCTCC CGGCAGTCCG TGCAGAACAC ACTCGCCGAG ATGGCACGCC GGATCGACGT CGCACGGGTG TACACACACG CGCTGGTCGA CCGTGCCATC GCGGGCGAGA GCAACCTGAT CGCCGAGGTG TGCTTCGCCA AGAACACCGC TGTCGAGGCC GGTGAATGGG TGGCCAATCA AGGCGTCCAA TTATTCGGGG GCATGGGCTA TATGGCCGAG AGCGAAATCG AACGGCAGTA TCGCGATATG CGCATCATCG GGATCGGTGG TGGCACCACC GAAATCCTCA CCTCCCTGGC CGCCAAGCTC CTGGGATTCC AGTCA
Details for MyabA.00247.c.A1.GE30268
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAVNSWN TPERKALRET VRDFAEREIL PNVNEWEREG LLPRELHRKA GDLGLLGPGS PEAVGGGGGD AIDPVIVCEE LHYAGVPGGV FASLFTCGIS TPHMIASGDQ RLIDNYVKPT LAGELIGSLA ITEPGGGSDV GHLTTSAKRD GDHYIVNGAK TFITSGVRAD YVVTAVRTGG PGAAGVSLLL IDKDTPGFEV ARKLDKMGWR SSDTAELSFT DVRVPAENLV GSENTGFAQI AGAFVSERIG LAAXAYASAQ RXLXLTVEWC XNRXXFXRPL XXGSXCRTHS XRWXAXSTSX GXTXTRWSXX PSXXXXXDXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttttgtttac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgac cgccgtgaat tcttggaaca cgcccgaacg caaggcactt cgggagaccg tgcgtgattt cgccgagcgc gagatcctgc cgaatgtcaa cgaatgggaa cgtgaggggc tgctcccgcg cgaattgcac cgcaaggccg gtgatctggg cctgctcggg cccggctcgc ccgaagcggt cggcggcgga ggtggcgatg ccatcgaccc ggtgatcgtg tgcgaggaac tgcactacgc gggcgtgccc ggtggcgtgt tcgcctccct gttcacctgt gggatctcca ccccgcacat gatcgcctcc ggcgatcagc ggctcatcga caattatgtg aagccaaccc tggccgggga actgatcggc tcgcttgcca tcaccgaacc cggcggcggg tccgatgtcg gtcacctgac cacatcggcg aagcgggacg gcgaccacta catcgtcaac ggcgccaaga ccttcatcac ctccggtgta cgcgccgact atgtggtgac cgcggtccgc accggcggcc cgggagccgc cggagtttcg ctgctcctga tcgacaagga cacaccggga tttgaggtgg ctcgcaagct cgacaagatg ggctggcgtt cctcggacac cgccgagctg tccttcaccg acgtgcgtgt acccgccgaa aacctggtgg ggtccgagaa caccggtttc gcacagatcg cgggcgcctt cgtctcggaa cgcatcggtc ttgccgcgca ngcntatgcn agcgcgcagc gctgnctgga nctgaccgtg gagtggtgcc ncaaccgggn nannttcngt cggccactct nnnccggnag tcngtgcaga acacactcnn cgagatggcn cgccngatcg acgtcncncg ggnntacacn cacncgctgg tcgancntnc catcnnngnn nnagcnnnnt gatnnncnnn gnnnnntncn nnnnnncncn nnnnnngngg nnnnngnnnn nnnnnnnnnn tnnnnnntnn gggnntnnnn nnntngnnnn ncnaannnnn nnnnnnnnnn nnnnnnnnna nnnnnnnnnn ngnnnnnnnc naannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAVNSWN TPERKALRET VRDFAEREIL PNVNEWEREG LLPRELHRKA GDLGLLGPGS PEAVGGGGGD AIDPVIVCEE LHYAGVPGGV FASLFTCGIS TPHMIASGDQ RLIDNYVKPT LAGELIGSLA ITEPGGGSDV GHLTTSAKRD GDHYIVNGAK TFITSGVRAD YVVTAVRTGG PGAAGVSLLL IDKDTPGFEV ARKLDKMGWR SSDTAELSFT DVRVPAENLV GSENTGFAQI AGAFVSERIG LAAQAYASAQ RCLDLTVEWC RNRETFGRPL ISRQSVQNTL AEMARRIDVA RVYTHALVDR AIAGESNLIA EVCFAKNTAV EAGEWVANQG VQLFGGMGYM AESEIERQYR DMRIIGIGGG TTEILTSLAA KLLGFQS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgcc gtgaattctt ggaacacgcc cgaacgcaag gcacttcggg agaccgtgcg tgatttcgcc gagcgcgaga tcctgccgaa tgtcaacgaa tgggaacgtg aggggctgct cccgcgcgaa ttgcaccgca aggccggtga tctgggcctg ctcgggcccg gctcgcccga agcggtcggc ggcggaggtg gcgatgccat cgacccggtg atcgtgtgcg aggaactgca ctacgcgggc gtgcccggtg gcgtgttcgc ctccctgttc acctgtggga tctccacccc gcacatgatc gcctccggcg atcagcggct catcgacaat tatgtgaagc caaccctggc cggggaactg atcggctcgc ttgccatcac cgaacccggc ggcgggtccg atgtcggtca cctgaccaca tcggcgaagc gggacggcga ccactacatc gtcaacggcg ccaagacctt catcacctcc ggtgtacgcg ccgactatgt ggtgaccgcg gtccgcaccg gcggcccggg agccgccgga gtttcgctgc tcctgatcga caaggacaca ccgggatttg aggtggctcg caagctcgac aagatgggct ggcgttcctc ggacaccgcc gagctgtcct tcaccgacgt gcgtgtaccc gccgaaaacc tggtggggtc cgagaacacc ggtttcgcac agatcgcggg cgccttcgtc tcggaacgca tcggtcttgc cgcgcaggcg tatgcgagcg cgcagcgctg cctggacctg accgtggagt ggtgccgcaa ccgggagacg ttcggtcggc cactcatctc ccggcagtcc gtgcagaaca cactcgccga gatggcacgc cggatcgacg tcgcacgggt gtacacacac gcgctggtcg accgtgccat cgcgggcgag agcaacctga tcgccgaggt gtgcttcgcc aagaacaccg ctgtcgaggc cggtgaatgg gtggccaatc aaggcgtcca attattcggg ggcatgggct atatggccga gagcgaaatc gaacggcagt atcgcgatat gcgcatcatc gggatcggtg gtggcaccac cgaaatcctc acctccctgg ccgccaagct cctgggattc cagtcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa