SSGCID: Target Page

MyabA.00247.d

Probable acyl-CoA dehydrogenase FadE

CENTER ID:	MyabA.00247.d
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00247.d.A1.GE30269	Full length( MyabA.00247.d )		1	379

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00247.d.A1.PS00923	Full length( MyabA.00247.d )		1	379

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1885
RefSeq:	YP_001702588.1
UniProt:	B1MNN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTFSSEHDDF RRTIRRYIEE KINPRVDEWE REQMMPLHEV IADMAKLGFV GLEYDPEYGG QGADHLFTLV LAEELGRVDH GSFPMAFGVH VAMATPSLHK HGSDELKREF LAPALRGEQI AAIAVTEPDA GSDVAGIKTH ARRDGDDWVI NGSKMFITNS VQADWLCVLA RTSDEGGYAG MSQIIVPTKA PGYEVRKLDK LGMHASDTGL ITFDDVRVPV SNTIGEIGRG FQQQMSQFVM ERMFGAYGIP ASVNRALQRT KEYALARHVF GKPLTKNQHL AYQYAGLAAR ADMLEVYNRD IAERYMAGEN VTRKVTIAKL TAGPLVREAG DWCLQVHGGM GYMTETWTSR FFRDQRLLSI GGGADEVMMR VLSQIDGFS

NT Sequence

ATGACGTTCT CGTCCGAACA CGACGACTTT CGCCGCACTA TCCGCAGGTA CATTGAGGAA AAGATCAACC CCCGCGTCGA CGAGTGGGAA CGCGAGCAGA TGATGCCGCT GCACGAGGTG ATCGCCGATA TGGCCAAGCT CGGATTCGTC GGCCTGGAAT ACGACCCTGA ATACGGCGGA CAAGGAGCGG ATCACCTCTT CACGCTGGTC CTTGCCGAGG AACTCGGCCG GGTGGATCAC GGGTCCTTTC CGATGGCCTT CGGGGTTCAC GTCGCGATGG CCACCCCCTC GCTGCACAAA CACGGCAGCG ATGAGCTCAA GCGCGAGTTC CTGGCTCCGG CCTTGCGCGG TGAGCAGATC GCGGCGATTG CGGTGACCGA GCCCGACGCC GGGTCCGATG TGGCGGGCAT CAAGACTCAT GCCCGGCGTG ACGGTGATGA CTGGGTGATC AACGGCTCGA AGATGTTCAT CACCAACTCC GTTCAGGCCG ACTGGTTGTG CGTGCTGGCC CGCACCTCCG ATGAGGGTGG CTACGCGGGC ATGAGTCAGA TCATCGTTCC GACCAAGGCC CCCGGGTACG AGGTCCGCAA GCTGGACAAG CTGGGCATGC ACGCCTCCGA CACCGGTCTG ATCACCTTCG ACGATGTCCG GGTGCCGGTG TCCAACACCA TCGGCGAGAT CGGCCGCGGG TTTCAGCAGC AGATGTCGCA GTTCGTCATG GAGCGCATGT TCGGCGCGTA CGGCATCCCC GCGAGTGTCA ACCGGGCGCT ACAGCGCACC AAGGAATATG CGTTGGCGCG CCACGTTTTT GGCAAGCCGC TGACCAAGAA CCAGCATCTG GCCTACCAAT ACGCGGGGCT GGCGGCGCGG GCCGACATGC TGGAGGTCTA CAACCGTGAC ATCGCCGAGC GGTACATGGC GGGGGAGAAC GTGACCCGCA AGGTCACCAT CGCCAAACTG ACCGCCGGGC CGCTGGTCCG CGAGGCCGGC GATTGGTGCC TGCAGGTGCA CGGCGGCATG GGGTATATGA CCGAGACGTG GACATCGCGA TTCTTCCGGG ATCAGCGTCT GCTCAGCATC GGCGGCGGTG CCGACGAGGT GATGATGCGG GTCCTATCCC AGATCGACGG ATTCTCC

Details for MyabA.00247.d.A1.GE30269

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	73

Validated AA Sequence

MAFGVHVAMA TPSLHKHGSD ELKREFLAPA LRGEQIAAIA VTEPDAGSDV AGIKTHARRD GDDWVINGSK MFITNSVQAD WLCVLARTSD EGGYAGMSQI IVPTKAPGYE VRKLDKLGMH ASDTGLITFD DVRVPVSNTI GEIGRGFQQQ MSQFVMERMF GAYGIPASVN RALQRTKEYA LARHVFGKPL TKNQHLAYQY AGLAARADML EVYNRDIAER YMAGENVTRK VTIAKLTAGP LVREAGDWCL QVHGGMGYMT ETWTSRFFRD QRLLSIGGGA DEVMMRVLSQ IDG

Validated NT Sequence

aatccgtcga tctgggatag gacccgcatc atcacctcgt cggcaccgcc gccgatgctg agcagacgct gatcccggaa gaatcgcgat gtccacgtct cggtcatata ccccatgccg ccgtgcacct gcaggcacca atcgccggcc tcgcggacca gcggcccggc ggtcagtttg gcgatggtga ccttgcgggt cacgttctcc cccgccatgt accgctcggc gatgtcacgg ttgtagacct ccagcatgtc ggcccgcgcc gccagccccg cgtattggta ggccagatgc tggttcttgg tcagcggctt gccaaaaacg tggcgcgcca acgcatattc cttggtgcgc tgtagcgccc ggttgacact cgcggggatg ccgtacgcgc cgaacatgcg ctccatgacg aactgcgaca tctgctgctg aaacccgcgg ccgatctcgc cgatggtgtt ggacaccggc acccggacat cgtcgaaggt gatcagaccg gtgtcggagg cgtgcatgcc cagcttgtcc agcttgcgga cctcgtaccc gggggccttg gtcggaacga tgatctgact catgcccgcg tagccaccct catcggaggt gcgggccagc acgcacaacc agtcggcctg aacggagttg gtgatgaaca tcttcgagcc gttgatcacc cagtcatcac cgtcacgccg ggcatgagtc ttgatgcccg ccacatcgga cccggcgtcg ggctcggtca ccgcaatcgc cgcgatctgc tcaccgcgca aggccggagc caggaactcg cgcttgagct catcgctgcc gtgtttgtgc agcgaggggg tggccatcgc gacgtgaacc ccgaaggcca tcggaaagga cccgtgatcc acccggccga gttcctcggc aaggaccagc gtgaagaggt gatccgctcc ttgtccgccg tattcagggt ngtattccag gccgacgaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTFSSEHDD FRRTIRRYIE EKINPRVDEW EREQMMPLHE VIADMAKLGF VGLEYDPEYG GQGADHLFTL VLAEELGRVD HGSFPMAFGV HVAMATPSLH KHGSDELKRE FLAPALRGEQ IAAIAVTEPD AGSDVAGIKT HARRDGDDWV INGSKMFITN SVQADWLCVL ARTSDEGGYA GMSQIIVPTK APGYEVRKLD KLGMHASDTG LITFDDVRVP VSNTIGEIGR GFQQQMSQFV MERMFGAYGI PASVNRALQR TKEYALARHV FGKPLTKNQH LAYQYAGLAA RADMLEVYNR DIAERYMAGE NVTRKVTIAK LTAGPLVREA GDWCLQVHGG MGYMTETWTS RFFRDQRLLS IGGGADEVMM RVLSQIDGFS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgttctcg tccgaacacg acgactttcg ccgcactatc cgcaggtaca ttgaggaaaa gatcaacccc cgcgtcgacg agtgggaacg cgagcagatg atgccgctgc acgaggtgat cgccgatatg gccaagctcg gattcgtcgg cctggaatac gaccctgaat acggcggaca aggagcggat cacctcttca cgctggtcct tgccgaggaa ctcggccggg tggatcacgg gtcctttccg atggccttcg gggttcacgt cgcgatggcc accccctcgc tgcacaaaca cggcagcgat gagctcaagc gcgagttcct ggctccggcc ttgcgcggtg agcagatcgc ggcgattgcg gtgaccgagc ccgacgccgg gtccgatgtg gcgggcatca agactcatgc ccggcgtgac ggtgatgact gggtgatcaa cggctcgaag atgttcatca ccaactccgt tcaggccgac tggttgtgcg tgctggcccg cacctccgat gagggtggct acgcgggcat gagtcagatc atcgttccga ccaaggcccc cgggtacgag gtccgcaagc tggacaagct gggcatgcac gcctccgaca ccggtctgat caccttcgac gatgtccggg tgccggtgtc caacaccatc ggcgagatcg gccgcgggtt tcagcagcag atgtcgcagt tcgtcatgga gcgcatgttc ggcgcgtacg gcatccccgc gagtgtcaac cgggcgctac agcgcaccaa ggaatatgcg ttggcgcgcc acgtttttgg caagccgctg accaagaacc agcatctggc ctaccaatac gcggggctgg cggcgcgggc cgacatgctg gaggtctaca accgtgacat cgccgagcgg tacatggcgg gggagaacgt gacccgcaag gtcaccatcg ccaaactgac cgccgggccg ctggtccgcg aggccggcga ttggtgcctg caggtgcacg gcggcatggg gtatatgacc gagacgtgga catcgcgatt cttccgggat cagcgtctgc tcagcatcgg cggcggtgcc gacgaggtga tgatgcgggt cctatcccag atcgacggat tctccaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MyabA.00247.d.A1.PS00923

PURIFICATION DATe:	12/29/2010
CONCENTRATION:	35.36mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGTTCTCG TCCGAACACG ACGACTTTCG CCGCACTATC CGCAGGTACA TTGAGGAAAA GATCAACCCC CGCGTCGACG AGTGGGAACG CGAGCAGATG ATGCCGCTGC ACGAGGTGAT CGCCGATATG GCCAAGCTCG GATTCGTCGG CCTGGAATAC GACCCTGAAT ACGGCGGACA AGGAGCGGAT CACCTCTTCA CGCTGGTCCT TGCCGAGGAA CTCGGCCGGG TGGATCACGG GTCCTTTCCG ATGGCCTTCG GGGTTCACGT CGCGATGGCC ACCCCCTCGC TGCACAAACA CGGCAGCGAT GAGCTCAAGC GCGAGTTCCT GGCTCCGGCC TTGCGCGGTG AGCAGATCGC GGCGATTGCG GTGACCGAGC CCGACGCCGG GTCCGATGTG GCGGGCATCA AGACTCATGC CCGGCGTGAC GGTGATGACT GGGTGATCAA CGGCTCGAAG ATGTTCATCA CCAACTCCGT TCAGGCCGAC TGGTTGTGCG TGCTGGCCCG CACCTCCGAT GAGGGTGGCT ACGCGGGCAT GAGTCAGATC ATCGTTCCGA CCAAGGCCCC CGGGTACGAG GTCCGCAAGC TGGACAAGCT GGGCATGCAC GCCTCCGACA CCGGTCTGAT CACCTTCGAC GATGTCCGGG TGCCGGTGTC CAACACCATC GGCGAGATCG GCCGCGGGTT TCAGCAGCAG ATGTCGCAGT TCGTCATGGA GCGCATGTTC GGCGCGTACG GCATCCCCGC GAGTGTCAAC CGGGCGCTAC AGCGCACCAA GGAATATGCG TTGGCGCGCC ACGTTTTTGG CAAGCCGCTG ACCAAGAACC AGCATCTGGC CTACCAATAC GCGGGGCTGG CGGCGCGGGC CGACATGCTG GAGGTCTACA ACCGTGACAT CGCCGAGCGG TACATGGCGG GGGAGAACGT GACCCGCAAG GTCACCATCG CCAAACTGAC CGCCGGGCCG CTGGTCCGCG AGGCCGGCGA TTGGTGCCTG CAGGTGCACG GCGGCATGGG GTATATGACC GAGACGTGGA CATCGCGATT CTTCCGGGAT CAGCGTCTGC TCAGCATCGG CGGCGGTGCC GACGAGGTGA TGATGCGGGT CCTATCCCAG ATCGACGGAT TCTCCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA