MyulA.00247.a

Acyl-CoA dehydrogenase FadE13

CENTER ID: MyulA.00247.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00247.a.A1.GE29663 Full length( MyulA.00247.a ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.5432
RefSeq: YP_908107.1
UniProt: A0PWB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIWNTPERE ELRKSVRSFA EREVLPHIDE WERTGELPLD LHRRAGAAGL LGAGLPESAG GGGGDGADSV IICEQMHQSG APGGVFASLF TCGIAVPHMI ASGDERLIEE FVRPTLAGNK IGALAITEPG GGSDVGHLRT SAVREGDHFI VNGAKTYITS GVRADFVVTA VRTGGPGAGG VSLLVVEKGT PGFVVSRKLD KMGWRSSDTA ELSYTDARIP AANLVGAENS GFAQIAQAFV SERIALAAQA YSSAQRCLDI TVQWCRDRET FGRPLISRQS VQNTLAEMAR RIDVARVYSR SVVERQLAGE HDLIPQVCFA KNTAVEAGEW VANQAVQLFG GMGYMAESEI ERQYRDMRIL GIGGGTTEIL TALAAKLLGY QS
NT Sequence
ATGAGCATCT GGAACACCCC CGAGCGCGAA GAGTTGCGCA AGTCGGTGCG CTCATTCGCC GAGCGGGAAG TGCTACCGCA CATCGACGAA TGGGAACGCA CCGGCGAGTT GCCCCTTGAC CTGCATCGCC GCGCCGGGGC GGCCGGCCTG TTGGGCGCCG GACTGCCCGA ATCCGCTGGC GGGGGCGGCG GCGACGGCGC CGACTCGGTG ATCATCTGCG AACAGATGCA CCAATCGGGT GCCCCGGGTG GGGTTTTCGC ATCGCTGTTC ACCTGCGGTA TCGCGGTGCC GCACATGATT GCCTCGGGCG ATGAGCGACT GATCGAGGAG TTCGTTCGGC CCACGCTGGC CGGGAACAAA ATCGGCGCGC TTGCCATCAC CGAACCCGGC GGCGGCTCGG ATGTGGGGCA TCTGCGGACC AGCGCGGTGC GCGAAGGTGA CCACTTCATC GTCAACGGCG CCAAGACCTA CATCACCTCC GGAGTACGCG CCGACTTCGT GGTCACCGCG GTCCGCACCG GAGGCCCCGG AGCCGGTGGG GTTTCGCTGT TGGTCGTGGA AAAGGGCACC CCGGGGTTCG TTGTCAGCCG CAAGCTGGAC AAGATGGGCT GGCGCTCCTC GGACACCGCC GAGCTGTCCT ACACCGATGC GCGGATTCCC GCGGCTAACC TCGTCGGCGC CGAGAACAGC GGCTTCGCCC AGATCGCGCA GGCGTTTGTC TCCGAGCGCA TCGCGCTTGC CGCACAGGCG TATTCGAGCG CGCAGCGATG CCTGGACATC ACCGTGCAAT GGTGCCGCGA CCGGGAAACA TTCGGCCGCC CACTGATCTC GCGCCAATCG GTGCAGAACA CGCTGGCCGA GATGGCCCGC CGCATCGACG TCGCCCGGGT CTACTCGCGC AGCGTGGTGG AGCGCCAACT CGCCGGCGAG CACGACCTGA TCCCGCAGGT CTGCTTCGCC AAGAACACCG CCGTCGAGGC CGGCGAATGG GTAGCCAATC AAGCCGTTCA GCTGTTCGGC GGCATGGGCT ACATGGCCGA ATCCGAAATC GAACGCCAAT ACCGGGACAT GCGAATCCTG GGCATTGGCG GTGGCACCAC CGAAATCCTG ACCGCACTGG CCGCCAAACT CCTGGGGTAT CAATCA
Details for MyulA.00247.a.A1.GE29663
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 58
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIWNTPER EELRKSVRSF AEREVLPHID EWERTGELPL DLHRRAGAAG LLGAGLPESA GGGGGDGADS VIICEQMHQS GAPGGVFASL FTCGIAVPHM IASGDERLIE EFVRPTLAGN KIGALAITEP GGGSDVGHLR XSAVREGDHF IVXGXXTYIT SGVRADFXXT AVRTGGPGAG GXSLXVVEKG TPGXVVSRKL XXXXAXLLGX RXXXXXXXXX XXXNXXXXXX XXXPXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXKX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
tttgtttnnc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcat ctggaacacc cccgagcgcg aagagttgcg caagtcggtg cgctcattcg ccgagcggga agtgctaccg cacatcgacg aatgggaacg caccggcgag ttgccccttg acctgcatcg ccgcgccggg gcggccggcc tgttgggcgc cggactgccc gaatccgctg gcgggggcgg cggcgacggc gccgactcgg tgatcatctg cgaacagatg caccaatcgg gtgccccggg tggggttttc gcatcgctgt tcacctgcgg tatcgcggtg ccgcacatga ttgcctcggg cgatgagcga ctgatcgagg agttcgttcg gcccacgctg gccgggaaca aaatcggcgc gcttgccatc accgaacccg gcggcggctc ggatgtgggg catctgcgga ncagcgcggt gcgcgaaggt gaccacttca tcgtcnacgg cgncnagacc tacatcacct ccggagtacg cgccgacttc ntggncaccg cggtccgcac cggaggcccc ggagccggtg ggnnttcgct gntggtcgtg gaaaagggca ccccggggnt cgttgtcagc cgcaagctng annnannnng ggctgnnctc ctcggannnc gccnanctgn ccnnnnnnga tgnncngnnt ncngnnnnaa ccnnntcgnn nncnanaann nnngntnncc cnnnnncnnn nangnnnngn nnncnnnnng natnnntnnn nnnnagnnnn nttnnnnnnn gnnnnngatn cngnnnnnnn cgcanngnnn nnnnnnnngn aannnttnnn nnnnccnntn gnnnnnaaan nnnnggnnnn annnnnnncn nnnnnnnnnn nnncnnnnnn nnnnnnnnnn nnngngnngn nnnnnnncnn nnnnnnnnnn nnnnnaannn nnncnnnnnn nnnnnnnngn nnannnnnnn nannnnntnn nnnnnnnnnn ngnnnnnnnn gnnaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnngnc nnnnnnnnnn nnnnnnaann nccnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIWNTPER EELRKSVRSF AEREVLPHID EWERTGELPL DLHRRAGAAG LLGAGLPESA GGGGGDGADS VIICEQMHQS GAPGGVFASL FTCGIAVPHM IASGDERLIE EFVRPTLAGN KIGALAITEP GGGSDVGHLR TSAVREGDHF IVNGAKTYIT SGVRADFVVT AVRTGGPGAG GVSLLVVEKG TPGFVVSRKL DKMGWRSSDT AELSYTDARI PAANLVGAEN SGFAQIAQAF VSERIALAAQ AYSSAQRCLD ITVQWCRDRE TFGRPLISRQ SVQNTLAEMA RRIDVARVYS RSVVERQLAG EHDLIPQVCF AKNTAVEAGE WVANQAVQLF GGMGYMAESE IERQYRDMRI LGIGGGTTEI LTALAAKLLG YQS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcatctgg aacacccccg agcgcgaaga gttgcgcaag tcggtgcgct cattcgccga gcgggaagtg ctaccgcaca tcgacgaatg ggaacgcacc ggcgagttgc cccttgacct gcatcgccgc gccggggcgg ccggcctgtt gggcgccgga ctgcccgaat ccgctggcgg gggcggcggc gacggcgccg actcggtgat catctgcgaa cagatgcacc aatcgggtgc cccgggtggg gttttcgcat cgctgttcac ctgcggtatc gcggtgccgc acatgattgc ctcgggcgat gagcgactga tcgaggagtt cgttcggccc acgctggccg ggaacaaaat cggcgcgctt gccatcaccg aacccggcgg cggctcggat gtggggcatc tgcggaccag cgcggtgcgc gaaggtgacc acttcatcgt caacggcgcc aagacctaca tcacctccgg agtacgcgcc gacttcgtgg tcaccgcggt ccgcaccgga ggccccggag ccggtggggt ttcgctgttg gtcgtggaaa agggcacccc ggggttcgtt gtcagccgca agctggacaa gatgggctgg cgctcctcgg acaccgccga gctgtcctac accgatgcgc ggattcccgc ggctaacctc gtcggcgccg agaacagcgg cttcgcccag atcgcgcagg cgtttgtctc cgagcgcatc gcgcttgccg cacaggcgta ttcgagcgcg cagcgatgcc tggacatcac cgtgcaatgg tgccgcgacc gggaaacatt cggccgccca ctgatctcgc gccaatcggt gcagaacacg ctggccgaga tggcccgccg catcgacgtc gcccgggtct actcgcgcag cgtggtggag cgccaactcg ccggcgagca cgacctgatc ccgcaggtct gcttcgccaa gaacaccgcc gtcgaggccg gcgaatgggt agccaatcaa gccgttcagc tgttcggcgg catgggctac atggccgaat ccgaaatcga acgccaatac cgggacatgc gaatcctggg cattggcggt ggcaccaccg aaatcctgac cgcactggcc gccaaactcc tggggtatca atcaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa