MyabA.00247.g
Probable acyl CoA dehydrogenase
| CENTER ID: | MyabA.00247.g |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00247.g.A1.GE30272 | Full length( MyabA.00247.g ) | 1 | 399 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00247.g.A1.PS00948 | Full length( MyabA.00247.g ) | 1 | 399 |
Structures
3R7K
DEPOSITED: 3/22/2011
DETERMINATION: XRay
CLONE: MyabA.00247.g.A1.GE30272
PROTEIN: MyabA.00247.g.A1.PS00948
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.2123 |
| RefSeq: | YP_001702820.1 |
| UniProt: | B1MPB5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDAGTTTAS EDGAARFVVA PEAWTTPERR ALSQMARSFV EREIAPKLAE WEHVGEIPRD LHLNAAEVGL LGIGFPEEVG GSGGNAIDSA LVTEAILAAG GSTGVCAALF THGIALPHIA ANGSDALIER YVRPTLAGKM IGSLGVTEPG AGSDVANLRT RAVREGDTYV VNGAKTFITS GVRADFVTTA VRTGGPGYGG VSLLVIDKNS PGFEVSRRLD KMGWRCSDTA ELSFVDVRVP ADNLVGAENS GFLQIMQQFQ AERLGIAVQA YATAGRALDL AKSWARERET FGRPLTGRQI IRHKLAEMAR QVDVACTYTR AVMQRWLAGE DVVAEVSMAK NTAVYACDYV VNEAVQIFGG MGYMRESEIE RHYRDCRILG IGGGTNEIMN EVIAKRIGL
NT Sequence
ATGACGGACG CTGGCACGAC CACGGCAAGC GAGGATGGGG CAGCTCGTTT CGTTGTCGCC CCGGAGGCGT GGACCACGCC GGAGCGACGA GCGCTGAGCC AAATGGCGCG GTCTTTCGTG GAACGTGAAA TCGCGCCGAA GCTAGCGGAA TGGGAGCACG TGGGTGAGAT TCCACGCGAC CTGCATCTCA ATGCTGCCGA GGTGGGGCTT CTCGGAATCG GGTTCCCGGA AGAAGTCGGC GGCAGCGGCG GCAATGCGAT TGACTCTGCA CTGGTCACTG AGGCCATCCT GGCCGCAGGC GGGTCCACTG GCGTCTGCGC CGCCTTGTTC ACCCATGGCA TTGCCCTTCC CCACATTGCC GCCAATGGCT CCGACGCCCT GATTGAACGA TATGTCCGTC CTACGCTCGC AGGAAAGATG ATCGGCTCGC TGGGCGTTAC CGAGCCTGGG GCAGGCTCAG ATGTCGCGAA TCTGCGCACA CGCGCGGTGC GCGAGGGGGA CACCTACGTG GTCAACGGAG CAAAGACGTT CATCACCAGC GGAGTTCGAG CGGACTTCGT TACTACGGCG GTACGCACCG GCGGACCGGG TTACGGTGGA GTTTCATTGC TTGTGATCGA CAAGAATTCG CCTGGATTCG AAGTGTCCCG CCGGTTAGAC AAGATGGGGT GGCGATGTAG CGACACCGCC GAGTTGTCTT TCGTCGACGT TCGCGTACCG GCTGACAACC TGGTGGGGGC AGAGAACAGT GGGTTTTTGC AGATCATGCA GCAATTTCAG GCTGAACGGC TCGGCATCGC CGTCCAGGCG TACGCGACGG CGGGTCGGGC TCTCGACCTT GCCAAGAGTT GGGCGCGGGA ACGTGAAACG TTCGGTAGAC CCTTGACGGG GCGCCAGATC ATTCGCCATA AGCTTGCGGA GATGGCTCGA CAGGTCGACG TGGCGTGCAC CTACACCCGT GCGGTCATGC AGAGGTGGCT AGCGGGGGAG GATGTCGTTG CCGAGGTGTC GATGGCCAAG AACACCGCTG TGTATGCGTG CGACTACGTG GTCAATGAGG CGGTTCAGAT CTTCGGTGGG ATGGGCTACA TGCGTGAGTC TGAGATCGAG AGACACTATC GAGATTGCCG AATTCTCGGA ATAGGCGGCG GCACCAACGA AATCATGAAC GAGGTCATCG CCAAACGTAT CGGACTC
Details for MyabA.00247.g.A1.GE30272
| HARVESTED ON: | 8/31/2010 |
| SEQUENCED ON: | 9/7/2010 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 91 |
| PERCENT COVERAGE: | 75 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDAGTTTA SEDGAARFVV APEAWTTPER RALSQMARSF VEREIAPKLA EWEHVGEIPR DLHLNAAEVG LLGIGFPEEV GGSGGNAIDS ALVTEAILAA GGSTGVCAAL FTHGIALPHI AANGSDALIE RYVRPTLAGK MIGSLGVTEP GAGSDVANLR TRAVREGDTY VVNGAKTFIT SGVRADFVTT AVRTGGPGYG GVSLLVIDKN SPGFEVSRRL DKMGWRCXDT AELSFVDXRV PADNLVGAEN SGFLXIMQXF XXERXXIAXQ AYAXXVGLST LPXVGRGNVK RSXXP
Validated NT Sequence
ttttnntnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgga cgctggcacg accacggcaa gcgaggatgg ggcagctcgt ttcgttgtcg ccccggaggc gtggaccacg ccggagcgac gagcgctgag ccaaatggcg cggtctttcg tggaacgtga aatcgcgccg aagctagcgg aatgggagca cgtgggtgag attccacgcg acctgcatct caatgctgcc gaggtggggc ttctcggaat cgggttcccg gaagaagtcg gcggcagcgg cggcaatgcg attgactctg cactggtcac tgaggccatc ctggccgcag gcgggtccac tggcgtctgc gccgccttgt tcacccatgg cattgccctt ccccacattg ccgccaatgg ctccgacgcc ctgattgaac gatatgtccg tcctacgctc gcaggaaaga tgatcggctc gctgggcgtt accgagcctg gggcaggctc agatgtcgcg aatctgcgca cacgcgcggt gcgcgagggg gacacctacg tggtcaacgg agcaaagacg ttcatcacca gcggagttcg agcggacttc gttactacgg cggtacgcac cggcggaccg ggttacggtg gagtttcatt gcttgtgatc gacaagaatt cgcctggatt cgaagtgtcc cgccggttag acaagatggg gtggcgatgt ancgacaccg ccgagttgtc tttcgtcgac nttcgcgtac cggctgacaa cctggtgggg gcagagaaca gtgggttttt gcanatcatg cagcantttc nnnntgaacg gntcgncatc gccntccagg cgtacgcnan nnnggtcggg ctctcgacct tgccaanagt tgggcgcggg aacgtgaaac gttcggnann nccttgacgg gncnncngat cattcnncat aancnngcgn nnnnnggntc gacnngngac ntgncnngcn nnnnnnncnn nngngncntg cnnangnngn tnncngnnnn angtcnnnnn nnannnnncn angncnnnan nncnncnnnn nnnntannnn nnnnnnnngn nnnnnnnnnn nnnnnnnnnn nnggnnnnnn nnnnnnnnnn nnnnnnnnna nnnnnnnnnn nnnnnnnnnn nnannnnnnn nnnnnnnnng nnnnngnnnn nnnnnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDAGTTTA SEDGAARFVV APEAWTTPER RALSQMARSF VEREIAPKLA EWEHVGEIPR DLHLNAAEVG LLGIGFPEEV GGSGGNAIDS ALVTEAILAA GGSTGVCAAL FTHGIALPHI AANGSDALIE RYVRPTLAGK MIGSLGVTEP GAGSDVANLR TRAVREGDTY VVNGAKTFIT SGVRADFVTT AVRTGGPGYG GVSLLVIDKN SPGFEVSRRL DKMGWRCSDT AELSFVDVRV PADNLVGAEN SGFLQIMQQF QAERLGIAVQ AYATAGRALD LAKSWARERE TFGRPLTGRQ IIRHKLAEMA RQVDVACTYT RAVMQRWLAG EDVVAEVSMA KNTAVYACDY VVNEAVQIFG GMGYMRESEI ERHYRDCRIL GIGGGTNEIM NEVIAKRIGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggacgct ggcacgacca cggcaagcga ggatggggca gctcgtttcg ttgtcgcccc ggaggcgtgg accacgccgg agcgacgagc gctgagccaa atggcgcggt ctttcgtgga acgtgaaatc gcgccgaagc tagcggaatg ggagcacgtg ggtgagattc cacgcgacct gcatctcaat gctgccgagg tggggcttct cggaatcggg ttcccggaag aagtcggcgg cagcggcggc aatgcgattg actctgcact ggtcactgag gccatcctgg ccgcaggcgg gtccactggc gtctgcgccg ccttgttcac ccatggcatt gcccttcccc acattgccgc caatggctcc gacgccctga ttgaacgata tgtccgtcct acgctcgcag gaaagatgat cggctcgctg ggcgttaccg agcctggggc aggctcagat gtcgcgaatc tgcgcacacg cgcggtgcgc gagggggaca cctacgtggt caacggagca aagacgttca tcaccagcgg agttcgagcg gacttcgtta ctacggcggt acgcaccggc ggaccgggtt acggtggagt ttcattgctt gtgatcgaca agaattcgcc tggattcgaa gtgtcccgcc ggttagacaa gatggggtgg cgatgtagcg acaccgccga gttgtctttc gtcgacgttc gcgtaccggc tgacaacctg gtgggggcag agaacagtgg gtttttgcag atcatgcagc aatttcaggc tgaacggctc ggcatcgccg tccaggcgta cgcgacggcg ggtcgggctc tcgaccttgc caagagttgg gcgcgggaac gtgaaacgtt cggtagaccc ttgacggggc gccagatcat tcgccataag cttgcggaga tggctcgaca ggtcgacgtg gcgtgcacct acacccgtgc ggtcatgcag aggtggctag cgggggagga tgtcgttgcc gaggtgtcga tggccaagaa caccgctgtg tatgcgtgcg actacgtggt caatgaggcg gttcagatct tcggtgggat gggctacatg cgtgagtctg agatcgagag acactatcga gattgccgaa ttctcggaat aggcggcggc accaacgaaa tcatgaacga ggtcatcgcc aaacgtatcg gactcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.00247.g.A1.PS00948
| PURIFICATION DATe: | 1/26/2011 |
| CONCENTRATION: | 39.21mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 91 |
| PERCENT COVERAGE: | 75 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDAGTTTA SEDGAARFVV APEAWTTPER RALSQMARSF VEREIAPKLA EWEHVGEIPR DLHLNAAEVG LLGIGFPEEV GGSGGNAIDS ALVTEAILAA GGSTGVCAAL FTHGIALPHI AANGSDALIE RYVRPTLAGK MIGSLGVTEP GAGSDVANLR TRAVREGDTY VVNGAKTFIT SGVRADFVTT AVRTGGPGYG GVSLLVIDKN SPGFEVSRRL DKMGWRCXDT AELSFVDXRV PADNLVGAEN SGFLXIMQXF XXERXXIAXQ AYAXXVGLST LPXVGRGNVK RSXXP
Validated NT Sequence
ttttnntnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgga cgctggcacg accacggcaa gcgaggatgg ggcagctcgt ttcgttgtcg ccccggaggc gtggaccacg ccggagcgac gagcgctgag ccaaatggcg cggtctttcg tggaacgtga aatcgcgccg aagctagcgg aatgggagca cgtgggtgag attccacgcg acctgcatct caatgctgcc gaggtggggc ttctcggaat cgggttcccg gaagaagtcg gcggcagcgg cggcaatgcg attgactctg cactggtcac tgaggccatc ctggccgcag gcgggtccac tggcgtctgc gccgccttgt tcacccatgg cattgccctt ccccacattg ccgccaatgg ctccgacgcc ctgattgaac gatatgtccg tcctacgctc gcaggaaaga tgatcggctc gctgggcgtt accgagcctg gggcaggctc agatgtcgcg aatctgcgca cacgcgcggt gcgcgagggg gacacctacg tggtcaacgg agcaaagacg ttcatcacca gcggagttcg agcggacttc gttactacgg cggtacgcac cggcggaccg ggttacggtg gagtttcatt gcttgtgatc gacaagaatt cgcctggatt cgaagtgtcc cgccggttag acaagatggg gtggcgatgt ancgacaccg ccgagttgtc tttcgtcgac nttcgcgtac cggctgacaa cctggtgggg gcagagaaca gtgggttttt gcanatcatg cagcantttc nnnntgaacg gntcgncatc gccntccagg cgtacgcnan nnnggtcggg ctctcgacct tgccaanagt tgggcgcggg aacgtgaaac gttcggnann nccttgacgg gncnncngat cattcnncat aancnngcgn nnnnnggntc gacnngngac ntgncnngcn nnnnnnncnn nngngncntg cnnangnngn tnncngnnnn angtcnnnnn nnannnnncn angncnnnan nncnncnnnn nnnntannnn nnnnnnnngn nnnnnnnnnn nnnnnnnnnn nnggnnnnnn nnnnnnnnnn nnnnnnnnna nnnnnnnnnn nnnnnnnnnn nnannnnnnn nnnnnnnnng nnnnngnnnn nnnnnnnnaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDAGTTTA SEDGAARFVV APEAWTTPER RALSQMARSF VEREIAPKLA EWEHVGEIPR DLHLNAAEVG LLGIGFPEEV GGSGGNAIDS ALVTEAILAA GGSTGVCAAL FTHGIALPHI AANGSDALIE RYVRPTLAGK MIGSLGVTEP GAGSDVANLR TRAVREGDTY VVNGAKTFIT SGVRADFVTT AVRTGGPGYG GVSLLVIDKN SPGFEVSRRL DKMGWRCSDT AELSFVDVRV PADNLVGAEN SGFLQIMQQF QAERLGIAVQ AYATAGRALD LAKSWARERE TFGRPLTGRQ IIRHKLAEMA RQVDVACTYT RAVMQRWLAG EDVVAEVSMA KNTAVYACDY VVNEAVQIFG GMGYMRESEI ERHYRDCRIL GIGGGTNEIM NEVIAKRIGL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGACGCT GGCACGACCA CGGCAAGCGA GGATGGGGCA GCTCGTTTCG TTGTCGCCCC GGAGGCGTGG ACCACGCCGG AGCGACGAGC GCTGAGCCAA ATGGCGCGGT CTTTCGTGGA ACGTGAAATC GCGCCGAAGC TAGCGGAATG GGAGCACGTG GGTGAGATTC CACGCGACCT GCATCTCAAT GCTGCCGAGG TGGGGCTTCT CGGAATCGGG TTCCCGGAAG AAGTCGGCGG CAGCGGCGGC AATGCGATTG ACTCTGCACT GGTCACTGAG GCCATCCTGG CCGCAGGCGG GTCCACTGGC GTCTGCGCCG CCTTGTTCAC CCATGGCATT GCCCTTCCCC ACATTGCCGC CAATGGCTCC GACGCCCTGA TTGAACGATA TGTCCGTCCT ACGCTCGCAG GAAAGATGAT CGGCTCGCTG GGCGTTACCG AGCCTGGGGC AGGCTCAGAT GTCGCGAATC TGCGCACACG CGCGGTGCGC GAGGGGGACA CCTACGTGGT CAACGGAGCA AAGACGTTCA TCACCAGCGG AGTTCGAGCG GACTTCGTTA CTACGGCGGT ACGCACCGGC GGACCGGGTT ACGGTGGAGT TTCATTGCTT GTGATCGACA AGAATTCGCC TGGATTCGAA GTGTCCCGCC GGTTAGACAA GATGGGGTGG CGATGTAGCG ACACCGCCGA GTTGTCTTTC GTCGACGTTC GCGTACCGGC TGACAACCTG GTGGGGGCAG AGAACAGTGG GTTTTTGCAG ATCATGCAGC AATTTCAGGC TGAACGGCTC GGCATCGCCG TCCAGGCGTA CGCGACGGCG GGTCGGGCTC TCGACCTTGC CAAGAGTTGG GCGCGGGAAC GTGAAACGTT CGGTAGACCC TTGACGGGGC GCCAGATCAT TCGCCATAAG CTTGCGGAGA TGGCTCGACA GGTCGACGTG GCGTGCACCT ACACCCGTGC GGTCATGCAG AGGTGGCTAG CGGGGGAGGA TGTCGTTGCC GAGGTGTCGA TGGCCAAGAA CACCGCTGTG TATGCGTGCG ACTACGTGGT CAATGAGGCG GTTCAGATCT TCGGTGGGAT GGGCTACATG CGTGAGTCTG AGATCGAGAG ACACTATCGA GATTGCCGAA TTCTCGGAAT AGGCGGCGGC ACCAACGAAA TCATGAACGA GGTCATCGCC AAACGTATCG GACTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA