SSGCID: Target Page

MypaA.00247.d

FadE13

CENTER ID:	MypaA.00247.d
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00247.d.A1.GE29281	Full length( MypaA.00247.d )		1	382

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00247.d.A1.PS00719	Full length( MypaA.00247.d )		1	382

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.954
RefSeq:	NP_959847.1
UniProt:	Q742C4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLWTTPERE QLRKTVRSFA EREILPHVDE WERSGELPRE LHRSAGAAGL LGAGFPESVG GGGGDGADAV IICEEMHQAG APGGVFASLF TCGIAVPHMI ASGDQRLIEE FVRPTLAGEK IGSLAITEPG GGSDVGHLRT SAVRDGDHYI VNGAKTYITS GVRADYVVTA VRTGGPGAAG VSLLVVEKGT PGFEVTRKLD KMGWRSSDTA ELSYTDVRVP VSNLVGAENT GFAQIAQAFV SERIGLAAQA YSSAQRCLDL TVQWCRDRET FGRPLISRQS VQNTLAEMAR RIDVARVYSR NVVERQLAGE TNLIPQVCFA KNTAVEAGEW VANQAVQLFG GMGYMAESEV ERQYRDMRIL GIGGGTTEIL IALSAKLLGF QS

NT Sequence

GTGAATCTGT GGACCACCCC CGAGCGCGAA CAACTGCGAA AGACGGTGCG CTCGTTCGCC GAACGCGAGA TCCTTCCGCA CGTCGACGAG TGGGAGCGCA GCGGCGAGCT GCCCCGCGAA TTGCACCGCA GCGCCGGGGC GGCCGGGCTG TTGGGCGCCG GCTTTCCCGA ATCGGTCGGC GGCGGGGGCG GCGACGGCGC CGACGCGGTG ATCATCTGCG AGGAGATGCA TCAGGCCGGA GCGCCGGGCG GGGTGTTCGC GTCGTTGTTC ACCTGCGGCA TCGCGGTGCC GCACATGATC GCCTCCGGGG ATCAGCGGCT CATCGAGGAG TTCGTCCGGC CCACGCTGGC CGGCGAGAAG ATCGGTTCGC TGGCCATCAC CGAGCCGGGC GGCGGTTCGG ACGTCGGTCA CCTGCGGACC TCCGCGGTAC GCGATGGTGA TCACTACATC GTCAACGGCG CCAAGACCTA CATCACCTCC GGGGTGCGTG CCGACTATGT GGTGACCGCG GTGCGCACCG GCGGCCCTGG GGCGGCAGGG GTTTCGTTGC TGGTGGTCGA GAAGGGCACT CCCGGGTTCG AGGTGACGCG CAAGCTGGAC AAGATGGGCT GGCGCTCCTC GGACACCGCC GAGCTGTCCT ACACCGACGT CCGGGTACCG GTGTCCAACC TGGTCGGCGC CGAGAACACC GGGTTCGCCC AAATAGCCCA GGCCTTCGTC TCCGAGCGGA TCGGCCTTGC CGCACAAGCG TATTCGAGTG CGCAGCGGTG CCTGGACCTG ACGGTGCAGT GGTGTCGCGA CCGCGAGACG TTCGGCCGGC CGCTGATTTC GCGGCAGTCG GTGCAGAACA CGCTGGCCGA GATGGCCCGC CGCATCGACG TCGCCCGGGT GTATTCGCGC AACGTGGTGG AACGCCAGCT GGCCGGTGAG ACGAATCTGA TCCCGCAGGT GTGCTTCGCC AAGAACACGG CCGTGGAGGC CGGCGAATGG GTTGCCAACC AGGCCGTGCA GCTGTTCGGC GGCATGGGCT ACATGGCCGA ATCCGAGGTC GAACGCCAAT ACCGGGACAT GAGGATCCTG GGCATCGGCG GCGGCACCAC CGAAATCCTG ATCGCATTGT CCGCCAAACT GCTGGGATTC CAATCA

Details for MypaA.00247.d.A1.GE29281

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MVNLWTTPER EQLRKTVRSF AEREILPHVD EWERSGELPR ELHRSAGAAG LLGAGFPESV GGGGGDGADA VIICEEMHQA GAPGGVFASL FTCGIAVPHM IASGDQRLIE EFVRPTLAGE KIGSLAITEP GGGSDVGHLR TSAVRDGDHY IVNGAKTYIT SGVRADYVVT AVRTGGPGAA GVSLLVVEKG TPGFEVTRKL DKMGWRSSDT AELSYTDVRV PVSNLVGAEN TGFAQIAQAF VSERIGLAAQ AYSSAQRCLD LTVQWCRDRE TFGRPLISRQ SVQNTLAEMA RRIDVARVYS RNVVERQLAG ETNLIPQVCF AKNTAVEAGE WVANQAVQLF GGMGYMAESE VERQYRDMRI LGIGGGTTEI LIALSAKLLG

Validated NT Sequence

aatcccagca gtttggcgga caatgcgatc aggatttcgg tggtgccgcc gccgatgccc aggatcctca tgtcccggta ttggcgttcg acctcggatt cggccatgta gcccatgccg ccgaacagct gcacggcctg gttggcaacc cattcgccgg cctccacggc cgtgttcttg gcgaagcaca cctgcgggat cagattcgtc tcaccggcca gctggcgttc caccacgttg cgcgaataca cccgggcgac gtcgatgcgg cgggccatct cggccagcgt gttctgcacc gactgccgcg aaatcagcgg ccggccgaac gtctcgcggt cgcgacacca ctgcaccgtc aggtccaggc accgctgcgc actcgaatac gcttgtgcgg caaggccgat ccgctcggag acgaaggcct gggctatttg ggcgaacccg gtgttctcgg cgccgaccag gttggacacc ggtacccgga cgtcggtgta ggacagctcg gcggtgtccg aggagcgcca gcccatcttg tccagcttgc gcgtcacctc gaacccggga gtgcccttct cgaccaccag caacgaaacc cctgccgccc cagggccgcc ggtgcgcacc gcggtcacca catagtcggc acgcaccccg gaggtgatgt aggtcttggc gccgttgacg atgtagtgat caccatcgcg taccgcggag gtccgcaggt gaccgacgtc cgaaccgccg cccggctcgg tgatggccag cgaaccgatc ttctcgccgg ccagcgtggg ccggacgaac tcctcgatga gccgctgatc cccggaggcg atcatgtgcg gcaccgcgat gccgcaggtg aacaacgacg cgaacacccc gcccggcgct ccggcctgat gcatctcctc gcagatgatc accgcgtcgg cgccgtcgcc gcccccgccg ccgaccgatt cgggaaagcc ggcgcccaac agcccggccg ccccggcgct gcggtgcaat tcgcggggca gctcgccgct gcgctcccac tcgtcgacgt gcggaaggat ctcgcgttcg gcgaacgagc gcaccgtctt tcgcagttgt tcgcgctcgg gggtggtcca cagattcacc atcgaaccag gaccctgggt ctgagcttcc agggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNLWTTPE REQLRKTVRS FAEREILPHV DEWERSGELP RELHRSAGAA GLLGAGFPES VGGGGGDGAD AVIICEEMHQ AGAPGGVFAS LFTCGIAVPH MIASGDQRLI EEFVRPTLAG EKIGSLAITE PGGGSDVGHL RTSAVRDGDH YIVNGAKTYI TSGVRADYVV TAVRTGGPGA AGVSLLVVEK GTPGFEVTRK LDKMGWRSSD TAELSYTDVR VPVSNLVGAE NTGFAQIAQA FVSERIGLAA QAYSSAQRCL DLTVQWCRDR ETFGRPLISR QSVQNTLAEM ARRIDVARVY SRNVVERQLA GETNLIPQVC FAKNTAVEAG EWVANQAVQL FGGMGYMAES EVERQYRDMR ILGIGGGTTE ILIALSAKLL GFQS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatctg tggaccaccc ccgagcgcga acaactgcga aagacggtgc gctcgttcgc cgaacgcgag atccttccgc acgtcgacga gtgggagcgc agcggcgagc tgccccgcga attgcaccgc agcgccgggg cggccgggct gttgggcgcc ggctttcccg aatcggtcgg cggcgggggc ggcgacggcg ccgacgcggt gatcatctgc gaggagatgc atcaggccgg agcgccgggc ggggtgttcg cgtcgttgtt cacctgcggc atcgcggtgc cgcacatgat cgcctccggg gatcagcggc tcatcgagga gttcgtccgg cccacgctgg ccggcgagaa gatcggttcg ctggccatca ccgagccggg cggcggttcg gacgtcggtc acctgcggac ctccgcggta cgcgatggtg atcactacat cgtcaacggc gccaagacct acatcacctc cggggtgcgt gccgactatg tggtgaccgc ggtgcgcacc ggcggccctg gggcggcagg ggtttcgttg ctggtggtcg agaagggcac tcccgggttc gaggtgacgc gcaagctgga caagatgggc tggcgctcct cggacaccgc cgagctgtcc tacaccgacg tccgggtacc ggtgtccaac ctggtcggcg ccgagaacac cgggttcgcc caaatagccc aggccttcgt ctccgagcgg atcggccttg ccgcacaagc gtattcgagt gcgcagcggt gcctggacct gacggtgcag tggtgtcgcg accgcgagac gttcggccgg ccgctgattt cgcggcagtc ggtgcagaac acgctggccg agatggcccg ccgcatcgac gtcgcccggg tgtattcgcg caacgtggtg gaacgccagc tggccggtga gacgaatctg atcccgcagg tgtgcttcgc caagaacacg gccgtggagg ccggcgaatg ggttgccaac caggccgtgc agctgttcgg cggcatgggc tacatggccg aatccgaggt cgaacgccaa taccgggaca tgaggatcct gggcatcggc ggcggcacca ccgaaatcct gatcgcattg tccgccaaac tgctgggatt ccaatcaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MypaA.00247.d.A1.PS00719

PURIFICATION DATe:	8/3/2010
CONCENTRATION:	53.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAATCTG TGGACCACCC CCGAGCGCGA ACAACTGCGA AAGACGGTGC GCTCGTTCGC CGAACGCGAG ATCCTTCCGC ACGTCGACGA GTGGGAGCGC AGCGGCGAGC TGCCCCGCGA ATTGCACCGC AGCGCCGGGG CGGCCGGGCT GTTGGGCGCC GGCTTTCCCG AATCGGTCGG CGGCGGGGGC GGCGACGGCG CCGACGCGGT GATCATCTGC GAGGAGATGC ATCAGGCCGG AGCGCCGGGC GGGGTGTTCG CGTCGTTGTT CACCTGCGGC ATCGCGGTGC CGCACATGAT CGCCTCCGGG GATCAGCGGC TCATCGAGGA GTTCGTCCGG CCCACGCTGG CCGGCGAGAA GATCGGTTCG CTGGCCATCA CCGAGCCGGG CGGCGGTTCG GACGTCGGTC ACCTGCGGAC CTCCGCGGTA CGCGATGGTG ATCACTACAT CGTCAACGGC GCCAAGACCT ACATCACCTC CGGGGTGCGT GCCGACTATG TGGTGACCGC GGTGCGCACC GGCGGCCCTG GGGCGGCAGG GGTTTCGTTG CTGGTGGTCG AGAAGGGCAC TCCCGGGTTC GAGGTGACGC GCAAGCTGGA CAAGATGGGC TGGCGCTCCT CGGACACCGC CGAGCTGTCC TACACCGACG TCCGGGTACC GGTGTCCAAC CTGGTCGGCG CCGAGAACAC CGGGTTCGCC CAAATAGCCC AGGCCTTCGT CTCCGAGCGG ATCGGCCTTG CCGCACAAGC GTATTCGAGT GCGCAGCGGT GCCTGGACCT GACGGTGCAG TGGTGTCGCG ACCGCGAGAC GTTCGGCCGG CCGCTGATTT CGCGGCAGTC GGTGCAGAAC ACGCTGGCCG AGATGGCCCG CCGCATCGAC GTCGCCCGGG TGTATTCGCG CAACGTGGTG GAACGCCAGC TGGCCGGTGA GACGAATCTG ATCCCGCAGG TGTGCTTCGC CAAGAACACG GCCGTGGAGG CCGGCGAATG GGTTGCCAAC CAGGCCGTGC AGCTGTTCGG CGGCATGGGC TACATGGCCG AATCCGAGGT CGAACGCCAA TACCGGGACA TGAGGATCCT GGGCATCGGC GGCGGCACCA CCGAAATCCT GATCGCATTG TCCGCCAAAC TGCTGGGATT CCAATCAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA