| CENTER ID: | MytuD.01254.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | crystallized |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01254.a.B1.GE36901 | Full length( MytuD.01254.a ) | 1 | 418 | ||
| MytuD.01254.a.B2.GE36902 | Phosphopantothenoylcysteine decarboxylase | 9 | 193 | ||
| MytuD.01254.a.B3.GE36903 | Phosphopantothenate-cysteine ligase | 190 | 418 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01254.a.B3.PW37330 | Phosphopantothenate-cysteine ligase | 190 | 418 |
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127040 |
| PATRIC ID: | fig|83332.12.peg.1556 |
| RefSeq: | NP_215907.1 |
| TubercuList: | Rv1391 |
| MTB Network Portal: | Rv1391 |
| UniProt: | P9WNZ1 |
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 7/22/2013 |
| CONCENTRATION: | 33.6mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |