CENTER ID: | MysmA.01254.a |
ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
ASSOCIATED DISEASE: | |
CURRENT STATUS: | in PDB |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
IIIC |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
MysmA.01254.a.A2.GE37764 | Mature protein( MysmA.01254.a ) | 19 | 414 | ||
MysmA.01254.a.B1.GE36897 | Full length( MysmA.01254.a ) | 1 | 414 | ||
MysmA.01254.a.B2.GE36898 | Mature protein( MysmA.01254.a ) | 19 | 414 | ||
MysmA.01254.a.B2.GE37627 | Mature protein( MysmA.01254.a ) | 19 | 414 | ||
MysmA.01254.a.B3.GE36899 | Phosphopantothenoylcysteine decarboxylase | 5 | 189 | ||
MysmA.01254.a.B4.GE36900 | Phosphopantothenate-cysteine ligase | 186 | 414 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
MysmA.01254.a.B1.PW37336 | Full length( MysmA.01254.a ) | 1 | 414 |
RESOURCE | REFERENCE ID |
---|---|
OrthoMCL: | OG5_127040 |
PATRIC ID: | fig|246196.19.peg.3015 |
RefSeq: | YP_887370.1 |
UniProt: | A0QWT2 |
HARVESTED ON: | 2/5/2014 |
SEQUENCED ON: | 2/4/2014 |
EXPECTED MW: | 44kDa |
OBSERVED MW: | 44kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Insoluble |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with incomplete coverage |
PERCENT IDENTITY: | 85 |
PERCENT COVERAGE: | 87 |
HARVESTED ON: | 5/1/2013 |
SEQUENCED ON: | 3/3/2015 |
EXPECTED MW: | 44kDa |
OBSERVED MW: | 44kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with incomplete coverage |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 67 |
HARVESTED ON: | 5/1/2013 |
SEQUENCED ON: | 5/8/2013 |
EXPECTED MW: | 42kDa |
OBSERVED MW: | 42kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Insoluble |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with incomplete coverage |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 98 |
HARVESTED ON: | 1/13/2014 |
SEQUENCED ON: | 1/9/2014 |
EXPECTED MW: | 42kDa |
OBSERVED MW: | 40kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with incomplete coverage |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 98 |
HARVESTED ON: | 5/1/2013 |
SEQUENCED ON: | 3/3/2015 |
EXPECTED MW: | 20kDa |
OBSERVED MW: | 18kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 5/1/2013 |
SEQUENCED ON: | 3/3/2015 |
EXPECTED MW: | 25kDa |
OBSERVED MW: | 25kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
PURIFICATION DATe: | 7/29/2013 |
CONCENTRATION: | 28.5mg/ml |
OBSERVED MW: | 44kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 11 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 67 |