MysmA.01254.a
Phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
| CENTER ID: | MysmA.01254.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01254.a.A2.GE37764 | Mature protein( MysmA.01254.a ) | 19 | 414 | ||
| MysmA.01254.a.B1.GE36897 | Full length( MysmA.01254.a ) | 1 | 414 | ||
| MysmA.01254.a.B2.GE36898 | Mature protein( MysmA.01254.a ) | 19 | 414 | ||
| MysmA.01254.a.B2.GE37627 | Mature protein( MysmA.01254.a ) | 19 | 414 | ||
| MysmA.01254.a.B3.GE36899 | Phosphopantothenoylcysteine decarboxylase | 5 | 189 | ||
| MysmA.01254.a.B4.GE36900 | Phosphopantothenate-cysteine ligase | 186 | 414 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01254.a.B1.PW37336 | Full length( MysmA.01254.a ) | 1 | 414 |
Structures
4QJI
DEPOSITED: 6/3/2014
DETERMINATION: XRay
CLONE: MysmA.01254.a.B4.GE36900
PROTEIN: MysmA.01254.a.B4.PW37345
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.3015 |
| RefSeq: | YP_887370.1 |
| UniProt: | A0QWT2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSARKRIVVG VAGGIAAYKA CTVVRQLTEA GHSVRVVPTE SALRFVGAAT FEALSGNPVH TGVFTDVHEV QHVRIGQQAD LVVIAPATAD LLARAVAGRA DDLLTATLLT ARCPVLFAPA MHTEMWLHPA TVDNVATLRR RGAVVLEPAS GRLTGADSGP GRLPEAEEIT TLAQLLLERA DALPYDMAGV KALVTAGGTR EPLDPVRFIG NRSSGKQGYA VARVLAQRGA DVTLIAGNTA GLIDPAGVEM VHIGSATQLR DAVSKHAPDA NVLVMAAAVA DFRPAHVAAA KIKKGASEPS SIDLVRNDDV LAGAVRARAD GQLPNMRAIV GFAAETGDAN GDVLFHARAK LERKGCDLLV VNAVGENRAF EVDHNDGWLL SADGTESALE HGSKTLMATR IVDSIAAFLK SQDG
NT Sequence
GTGAGCGCGC GCAAGCGGAT CGTCGTCGGC GTCGCCGGCG GGATCGCCGC CTACAAGGCG TGCACTGTTG TCCGTCAACT CACCGAGGCT GGGCATTCGG TGCGGGTCGT CCCCACCGAG TCCGCGTTGC GCTTCGTCGG TGCCGCGACC TTCGAGGCGC TGTCCGGTAA CCCGGTGCAC ACCGGGGTGT TCACCGACGT CCACGAGGTG CAGCACGTCC GCATCGGCCA GCAGGCCGAC CTCGTCGTCA TCGCACCTGC CACCGCCGAT CTCCTGGCCC GCGCGGTCGC GGGGCGTGCC GACGATCTGC TGACCGCCAC GCTGCTCACC GCACGATGCC CCGTGCTGTT CGCGCCTGCC ATGCACACCG AGATGTGGTT GCACCCGGCC ACCGTCGACA ACGTGGCCAC GCTGCGCCGT CGCGGTGCGG TGGTGCTGGA ACCGGCGTCC GGACGTCTCA CAGGCGCCGA CAGCGGCCCG GGCCGGCTCC CTGAGGCCGA GGAGATCACC ACACTCGCGC AGCTGCTGCT GGAACGCGCC GACGCGCTTC CCTACGACAT GGCCGGGGTC AAGGCCCTGG TGACCGCTGG TGGTACGCGT GAGCCGCTCG ACCCGGTCCG TTTCATCGGC AACCGCAGCT CCGGCAAGCA GGGCTACGCC GTCGCGCGTG TGCTGGCCCA GCGTGGCGCC GACGTGACGC TCATCGCGGG CAACACGGCC GGTCTGATCG ATCCCGCGGG CGTCGAGATG GTCCACATCG GCTCGGCGAC CCAGCTGCGC GACGCGGTGT CCAAGCACGC ACCCGACGCG AACGTTCTGG TGATGGCCGC CGCGGTCGCC GACTTCCGTC CTGCGCACGT TGCCGCCGCG AAGATCAAGA AGGGCGCCTC GGAACCCAGC TCGATCGATC TTGTCCGCAA CGACGACGTG CTGGCCGGAG CGGTCCGCGC CAGGGCCGAC GGACAGTTGC CAAACATGCG CGCGATCGTC GGTTTCGCCG CGGAAACCGG GGACGCGAAC GGCGACGTGC TGTTCCACGC CAGGGCGAAA CTCGAGCGTA AGGGCTGTGA CCTGCTGGTC GTCAACGCCG TGGGGGAGAA CCGGGCATTC GAGGTGGACC ACAACGACGG ATGGTTGCTG AGTGCCGACG GCACCGAGTC CGCACTGGAG CACGGCTCGA AGACGCTCAT GGCCACCCGT ATCGTGGACT CGATTGCGGC CTTCCTGAAG AGCCAGGACG GG
Details for MysmA.01254.a.A2.GE37764
| HARVESTED ON: | 2/5/2014 |
| SEQUENCED ON: | 2/4/2014 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 85 |
| PERCENT COVERAGE: | 87 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKACTVVRQ LTEAGHSVRV VPTESALRFV GAATFEALSG NPVHTGVFTD VHEVQHVRIG QQADLVVIAP ATADLLARAV AGRADDLLTA TLLTARCPVL FAPAMHTEMW LHPATVDNVA TLRRRGAVVL EPASGRLTGA DSGPGRLPEA EEITTLAQLL LERADALPYD MAGVKALVTA GGTREPLDPV RFIGNRSSGK QGYAVARVLA QRGADVTLIA GNTAGLIDPA GVEMVHIGSA TQLRDAVSKH APDANVLVMA AAVADFRPAH VAAARSXXAS EPSSIDLVRN XXAGRXVXXK XXXVXXCXXS SXAAXXGXXR XXXXXXXXXX XXCXXXGXXX VXXX
Validated NT Sequence
cnnnnnnaat attttnttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaag gcgtgcactg ttgtccgtca actcaccgag gctgggcatt cggtgcgggt cgtccccacc gagtccgcgt tgcgcttcgt cggtgccgcg accttcgagg cgctgtccgg taacccggtg cacaccgggg tgttcaccga cgtccacgag gtgcagcacg tccgcatcgg ccagcaggcc gacctcgtcg tcatcgcacc tgccaccgcc gatctcctgg cccgcgcggt cgcggggcgt gccgacgatc tgctgaccgc cacgctgctc accgcacgat gccccgtgct gttcgcgcct gccatgcaca ccgagatgtg gttgcacccg gccaccgtcg acaacgtggc cacgctgcgc cgtcgcggtg cggtggtgct ggaaccggcg tccggacgtc tcacaggcgc cgacagcggc ccgggccggc tccctgaggc cgaggagatc accacactcg cgcagctgct gctggaacgc gccgacgcgc ttccctacga catggccggg gtcaaggccc tggtgaccgc tggtggtacg cgtgagccgc tcgacccggt ccgtttcatc ggcaaccgca gctccggcaa gcagggctac gccgtcgcgc gtgtgctggc ccagcgtggc gccgacgtga cgctcatcgc gggcaacacg gccggtctga tcgatcccgc gggcgtcgag atggtccaca tcggctcggc gacccagctg cgcgacgcgg tgtccaagca cgcacccgac gcgaacgttc tggtgatggc cgccgcggtc gccgacttcc gtcctgcgca cgttgccgcc gcgagatcan anngcgcctc ggaacccagc tcgatcgatc ttgtccgcaa cgannnngct ggccgnncgg tccnnnccaa ggnnannnnn gttnnnnnat gcncgcnatc gtcgntngcc gcggnaanng gancnnngcg anngnnnnnn ncnnnnnnnn nnntannnnt nnacnnctgt nnnancnggg gaannnnnnn agtgannnnn nnga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKACTVVRQ LTEAGHSVRV VPTESALRFV GAATFEALSG NPVHTGVFTD VHEVQHVRIG QQADLVVIAP ATADLLARAV AGRADDLLTA TLLTARCPVL FAPAMHTEMW LHPATVDNVA TLRRRGAVVL EPASGRLTGA DSGPGRLPEA EEITTLAQLL LERADALPYD MAGVKALVTA GGTREPLDPV RFIGNRSSGK QGYAVARVLA QRGADVTLIA GNTAGLIDPA GVEMVHIGSA TQLRDAVSKH APDANVLVMA AAVADFRPAH VAAAKIKKGA SEPSSIDLVR NDDVLAGAVR ARADGQLPNM RAIVGFAAET GDANGDVLFH ARAKLERKGC DLLVVNAVGE NRAFEVDHND GWLLSADGTE SALEHGSKTL MATRIVDSIA AFLKSQDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggcgtgc actgttgtcc gtcaactcac cgaggctggg cattcggtgc gggtcgtccc caccgagtcc gcgttgcgct tcgtcggtgc cgcgaccttc gaggcgctgt ccggtaaccc ggtgcacacc ggggtgttca ccgacgtcca cgaggtgcag cacgtccgca tcggccagca ggccgacctc gtcgtcatcg cacctgccac cgccgatctc ctggcccgcg cggtcgcggg gcgtgccgac gatctgctga ccgccacgct gctcaccgca cgatgccccg tgctgttcgc gcctgccatg cacaccgaga tgtggttgca cccggccacc gtcgacaacg tggccacgct gcgccgtcgc ggtgcggtgg tgctggaacc ggcgtccgga cgtctcacag gcgccgacag cggcccgggc cggctccctg aggccgagga gatcaccaca ctcgcgcagc tgctgctgga acgcgccgac gcgcttccct acgacatggc cggggtcaag gccctggtga ccgctggtgg tacgcgtgag ccgctcgacc cggtccgttt catcggcaac cgcagctccg gcaagcaggg ctacgccgtc gcgcgtgtgc tggcccagcg tggcgccgac gtgacgctca tcgcgggcaa cacggccggt ctgatcgatc ccgcgggcgt cgagatggtc cacatcggct cggcgaccca gctgcgcgac gcggtgtcca agcacgcacc cgacgcgaac gttctggtga tggccgccgc ggtcgccgac ttccgtcctg cgcacgttgc cgccgcgaag atcaagaagg gcgcctcgga acccagctcg atcgatcttg tccgcaacga cgacgtgctg gccggagcgg tccgcgccag ggccgacgga cagttgccaa acatgcgcgc gatcgtcggt ttcgccgcgg aaaccgggga cgcgaacggc gacgtgctgt tccacgccag ggcgaaactc gagcgtaagg gctgtgacct gctggtcgtc aacgccgtgg gggagaaccg ggcattcgag gtggaccaca acgacggatg gttgctgagt gccgacggca ccgagtccgc actggagcac ggctcgaaga cgctcatggc cacccgtatc gtggactcga ttgcggcctt cctgaagagc caggacggga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.01254.a.B1.GE36897
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 67 |
Validated AA Sequence
MAHHHHHHMV SARKRIVVGV AGGIAAYKAC TVVRQLTEAG HSVRVVPTES ALRFVGAATF EALSGNPVHT GVFTDVHEVQ HVRIGQQADL VVIAPATADL LARAVAGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPASG RLTGADSGPG RLPEAEEITT LAQLLLERAD ALPYDMAGVK ALVTAGGTRE PLDPVRFIGN RSSGKQGYAV ARVLAQRGAD VTLIAGNTAG LIDPAGVEMV HIGSATQLRD AVSKHAPDAN VLV
Validated NT Sequence
atggctcacc accaccacca ccatatggtg agcgcgcgca agcggatcgt cgtcggcgtc gccggcggga tcgccgccta caaggcgtgc actgttgtcc gtcaactcac cgaggctggg cattcggtgc gggtcgtccc caccgagtcc gcgttgcgct tcgtcggtgc cgcgaccttc gaggcgctgt ccggtaaccc ggtgcacacc ggggtgttca ccgacgtcca cgaggtgcag cacgtccgca tcggccagca ggccgacctc gtcgtcatcg cacctgccac cgccgatctc ctggcccgcg cggtcgcggg gcgtgccgac gatctgctga ccgccacgct gctcaccgca cgatgccccg tgctgttcgc gcctgccatg cacaccgaga tgtggttgca cccggccacc gtcgacaacg tggccacgct gcgccgtcgc ggtgcggtgg tgctggaacc ggcgtccgga cgtctcacag gcgccgacag cggcccgggc cggctccctg aggccgagga gatcaccaca ctcgcgcagc tgctgctgga acgcgccgac gcgcttccct acgacatggc cggggtcaag gccctggtga ccgctggtgg tacgcgtgag ccgctcgacc cggtccgttt catcggcaac cgcagctccg gcaagcaggg ctacgccgtc gcgcgtgtgc tggcccagcg tggcgccgac gtgacgctca tcgcgggcaa cacggccggt ctgatcgatc ccgcgggcgt cgagatggtc cacatcggct cggcgaccca gctgcgcgac gcggtgtcca agcacgcacc cgacgcgaac gttctggtga ng
Expected Protein Sequence
MAHHHHHHMS ARKRIVVGVA GGIAAYKACT VVRQLTEAGH SVRVVPTESA LRFVGAATFE ALSGNPVHTG VFTDVHEVQH VRIGQQADLV VIAPATADLL ARAVAGRADD LLTATLLTAR CPVLFAPAMH TEMWLHPATV DNVATLRRRG AVVLEPASGR LTGADSGPGR LPEAEEITTL AQLLLERADA LPYDMAGVKA LVTAGGTREP LDPVRFIGNR SSGKQGYAVA RVLAQRGADV TLIAGNTAGL IDPAGVEMVH IGSATQLRDA VSKHAPDANV LVMAAAVADF RPAHVAAAKI KKGASEPSSI DLVRNDDVLA GAVRARADGQ LPNMRAIVGF AAETGDANGD VLFHARAKLE RKGCDLLVVN AVGENRAFEV DHNDGWLLSA DGTESALEHG SKTLMATRIV DSIAAFLKSQ DG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagcg cgcgcaagcg gatcgtcgtc ggcgtcgccg gcgggatcgc cgcctacaag gcgtgcactg ttgtccgtca actcaccgag gctgggcatt cggtgcgggt cgtccccacc gagtccgcgt tgcgcttcgt cggtgccgcg accttcgagg cgctgtccgg taacccggtg cacaccgggg tgttcaccga cgtccacgag gtgcagcacg tccgcatcgg ccagcaggcc gacctcgtcg tcatcgcacc tgccaccgcc gatctcctgg cccgcgcggt cgcggggcgt gccgacgatc tgctgaccgc cacgctgctc accgcacgat gccccgtgct gttcgcgcct gccatgcaca ccgagatgtg gttgcacccg gccaccgtcg acaacgtggc cacgctgcgc cgtcgcggtg cggtggtgct ggaaccggcg tccggacgtc tcacaggcgc cgacagcggc ccgggccggc tccctgaggc cgaggagatc accacactcg cgcagctgct gctggaacgc gccgacgcgc ttccctacga catggccggg gtcaaggccc tggtgaccgc tggtggtacg cgtgagccgc tcgacccggt ccgtttcatc ggcaaccgca gctccggcaa gcagggctac gccgtcgcgc gtgtgctggc ccagcgtggc gccgacgtga cgctcatcgc gggcaacacg gccggtctga tcgatcccgc gggcgtcgag atggtccaca tcggctcggc gacccagctg cgcgacgcgg tgtccaagca cgcacccgac gcgaacgttc tggtgatggc cgccgcggtc gccgacttcc gtcctgcgca cgttgccgcc gcgaagatca agaagggcgc ctcggaaccc agctcgatcg atcttgtccg caacgacgac gtgctggccg gagcggtccg cgccagggcc gacggacagt tgccaaacat gcgcgcgatc gtcggtttcg ccgcggaaac cggggacgcg aacggcgacg tgctgttcca cgccagggcg aaactcgagc gtaagggctg tgacctgctg gtcgtcaacg ccgtggggga gaaccgggca ttcgaggtgg accacaacga cggatggttg ctgagtgccg acggcaccga gtccgcactg gagcacggct cgaagacgct catggccacc cgtatcgtgg actcgattgc ggccttcctg aagagccagg acgggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MysmA.01254.a.B2.GE36898
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 5/8/2013 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMK ACTVVRQLTE AGHSVRVVPT ESALRFVGAA TFEALSGNPV HTGVFTDVHE VQHVRIGQQA DLVVIAPATA DLLARAVAGR ADDLLTATLL TARCPVLFAP AMHTEMWLHP ATVDNVATLR RRGAVVLEPA SGRLTGADSG PGRLPEAEEI TTLAQLLLER ADALPYDMAG VKALVTAGGT REPLDPVRFI GNRSSGKQGY AVARVLAQRG ADVTLIAGNT AGLIDPAGVE MVHIGSATQL RDAVSKHAPD ANVLVMAAAV ADFRPAHVAA AKIKKGASEP SSIDLVRNDD VLAGAVRARA DGQLPNMRAI VGFAAETGDA NGDVLFHARA KLERKGCDLL VVNAVGENRA FEVDHNDGWL LSADGTESAL EHGSKTLMAT RIVDSI
Validated NT Sequence
atggctcacc accaccacca ccatatgaag gcgtgcactg ttgtccgtca actcaccgag gctgggcatt cggtgcgggt cgtccccacc gagtccgcgt tgcgcttcgt cggtgccgcg accttcgagg cgctgtccgg taacccggtg cacaccgggg tgttcaccga cgtccacgag gtgcagcacg tccgcatcgg ccagcaggcc gacctcgtcg tcatcgcacc tgccaccgcc gatctcctgg cccgcgcggt cgcggggcgt gccgacgatc tgctgaccgc cacgctgctc accgcacgat gccccgtgct gttcgcgcct gccatgcaca ccgagatgtg gttgcacccg gccaccgtcg acaacgtggc cacgctgcgc cgtcgcggtg cggtggtgct ggaaccggcg tccggacgtc tcacaggcgc cgacagcggc ccgggccggc tccctgaggc cgaggagatc accacactcg cgcagctgct gctggaacgc gccgacgcgc ttccctacga catggccggg gtcaaggccc tggtgaccgc tggtggtacg cgtgagccgc tcgacccggt ccgtttcatc ggcaaccgca gctccggcaa gcagggctac gccgtcgcgc gtgtgctggc ccagcgtggc gccgacgtga cgctcatcgc gggcaacacg gccggtctga tcgatcccgc gggcgtcgag atggtccaca tcggctcggc gacccagctg cgcgacgcgg tgtccaagca cgcacccgac gcgaacgttc tggtgatggc cgccgcggtc gccgacttcc gtcctgcgca cgttgccgcc gcgaagatca agaagggcgc ctcggaaccc agctcgatcg atcttgtccg caacgacgac gtgctggccg gagcggtccg cgccagggcc gacggacagt tgccaaacat gcgcgcgatc gtcggtttcg ccgcggaaac cggggacgcg aacggcgacg tgctgttcca cgccagggcg aaactcgagc gtaagggctg tgacctgctg gtcgtcaacg ccgtggggga gaaccgggca ttcgaggtgg accacaacga cggatggttg ctgagtgccg acggcaccga gtccgcactg gagcacggct cgaagacgct catggccacc cgtatcgtgg actcgattg
Expected Protein Sequence
MAHHHHHHKA CTVVRQLTEA GHSVRVVPTE SALRFVGAAT FEALSGNPVH TGVFTDVHEV QHVRIGQQAD LVVIAPATAD LLARAVAGRA DDLLTATLLT ARCPVLFAPA MHTEMWLHPA TVDNVATLRR RGAVVLEPAS GRLTGADSGP GRLPEAEEIT TLAQLLLERA DALPYDMAGV KALVTAGGTR EPLDPVRFIG NRSSGKQGYA VARVLAQRGA DVTLIAGNTA GLIDPAGVEM VHIGSATQLR DAVSKHAPDA NVLVMAAAVA DFRPAHVAAA KIKKGASEPS SIDLVRNDDV LAGAVRARAD GQLPNMRAIV GFAAETGDAN GDVLFHARAK LERKGCDLLV VNAVGENRAF EVDHNDGWLL SADGTESALE HGSKTLMATR IVDSIAAFLK SQDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaggcgt gcactgttgt ccgtcaactc accgaggctg ggcattcggt gcgggtcgtc cccaccgagt ccgcgttgcg cttcgtcggt gccgcgacct tcgaggcgct gtccggtaac ccggtgcaca ccggggtgtt caccgacgtc cacgaggtgc agcacgtccg catcggccag caggccgacc tcgtcgtcat cgcacctgcc accgccgatc tcctggcccg cgcggtcgcg gggcgtgccg acgatctgct gaccgccacg ctgctcaccg cacgatgccc cgtgctgttc gcgcctgcca tgcacaccga gatgtggttg cacccggcca ccgtcgacaa cgtggccacg ctgcgccgtc gcggtgcggt ggtgctggaa ccggcgtccg gacgtctcac aggcgccgac agcggcccgg gccggctccc tgaggccgag gagatcacca cactcgcgca gctgctgctg gaacgcgccg acgcgcttcc ctacgacatg gccggggtca aggccctggt gaccgctggt ggtacgcgtg agccgctcga cccggtccgt ttcatcggca accgcagctc cggcaagcag ggctacgccg tcgcgcgtgt gctggcccag cgtggcgccg acgtgacgct catcgcgggc aacacggccg gtctgatcga tcccgcgggc gtcgagatgg tccacatcgg ctcggcgacc cagctgcgcg acgcggtgtc caagcacgca cccgacgcga acgttctggt gatggccgcc gcggtcgccg acttccgtcc tgcgcacgtt gccgccgcga agatcaagaa gggcgcctcg gaacccagct cgatcgatct tgtccgcaac gacgacgtgc tggccggagc ggtccgcgcc agggccgacg gacagttgcc aaacatgcgc gcgatcgtcg gtttcgccgc ggaaaccggg gacgcgaacg gcgacgtgct gttccacgcc agggcgaaac tcgagcgtaa gggctgtgac ctgctggtcg tcaacgccgt gggggagaac cgggcattcg aggtggacca caacgacgga tggttgctga gtgccgacgg caccgagtcc gcactggagc acggctcgaa gacgctcatg gccacccgta tcgtggactc gattgcggcc ttcctgaaga gccaggacgg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for MysmA.01254.a.B2.GE37627
| HARVESTED ON: | 1/13/2014 |
| SEQUENCED ON: | 1/9/2014 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMK ACTVVRQLTE AGHSVRVVPT ESALRFVGAA TFEALSGNPV HTGVFTDVHE VQHVRIGQQA DLVVIAPATA DLLARAVAGR ADDLLTATLL TARCPVLFAP AMHTEMWLHP ATVDNVATLR RRGAVVLEPA SGRLTGADSG PGRLPEAEEI TTLAQLLLER ADALPYDMAG VKALVTAGGT REPLDPVRFI GNRSSGKQGY AVARVLAQRG ADVTLIAGNT AGLIDPAGVE MVHIGSATQL RDAVSKHAPD ANVLVMAAAV ADFRPAHVAA AKIKKGASEP SSIDLVRNDD VLAGAVRARA DGQLPNMRAI VGFAAETGDA NGDVLFHARA KLERKGCDLL VVNAVGENRA FEVDHNDGWL LSADGTESAL EHGSKTLMAT RIVDSI
Validated NT Sequence
atggctcacc accaccacca ccatatgaag gcgtgcactg ttgtccgtca actcaccgag gctgggcatt cggtgcgggt cgtccccacc gagtccgcgt tgcgcttcgt cggtgccgcg accttcgagg cgctgtccgg taacccggtg cacaccgggg tgttcaccga cgtccacgag gtgcagcacg tccgcatcgg ccagcaggcc gacctcgtcg tcatcgcacc tgccaccgcc gatctcctgg cccgcgcggt cgcggggcgt gccgacgatc tgctgaccgc cacgctgctc accgcacgat gccccgtgct gttcgcgcct gccatgcaca ccgagatgtg gttgcacccg gccaccgtcg acaacgtggc cacgctgcgc cgtcgcggtg cggtggtgct ggaaccggcg tccggacgtc tcacaggcgc cgacagcggc ccgggccggc tccctgaggc cgaggagatc accacactcg cgcagctgct gctggaacgc gccgacgcgc ttccctacga catggccggg gtcaaggccc tggtgaccgc tggtggtacg cgtgagccgc tcgacccggt ccgtttcatc ggcaaccgca gctccggcaa gcagggctac gccgtcgcgc gtgtgctggc ccagcgtggc gccgacgtga cgctcatcgc gggcaacacg gccggtctga tcgatcccgc gggcgtcgag atggtccaca tcggctcggc gacccagctg cgcgacgcgg tgtccaagca cgcacccgac gcgaacgttc tggtgatggc cgccgcggtc gccgacttcc gtcctgcgca cgttgccgcc gcgaagatca agaagggcgc ctcggaaccc agctcgatcg atcttgtccg caacgacgac gtgctggccg gagcggtccg cgccagggcc gacggacagt tgccaaacat gcgcgcgatc gtcggtttcg ccgcggaaac cggggacgcg aacggcgacg tgctgttcca cgccagggcg aaactcgagc gtaagggctg tgacctgctg gtcgtcaacg ccgtggggga gaaccgggca ttcgaggtgg accacaacga cggatggttg ctgagtgccg acggcaccga gtccgcactg gagcacggct cgaagacgct catggccacc cgtatcgtgg actcgattg
Expected Protein Sequence
MAHHHHHHKA CTVVRQLTEA GHSVRVVPTE SALRFVGAAT FEALSGNPVH TGVFTDVHEV QHVRIGQQAD LVVIAPATAD LLARAVAGRA DDLLTATLLT ARCPVLFAPA MHTEMWLHPA TVDNVATLRR RGAVVLEPAS GRLTGADSGP GRLPEAEEIT TLAQLLLERA DALPYDMAGV KALVTAGGTR EPLDPVRFIG NRSSGKQGYA VARVLAQRGA DVTLIAGNTA GLIDPAGVEM VHIGSATQLR DAVSKHAPDA NVLVMAAAVA DFRPAHVAAA KIKKGASEPS SIDLVRNDDV LAGAVRARAD GQLPNMRAIV GFAAETGDAN GDVLFHARAK LERKGCDLLV VNAVGENRAF EVDHNDGWLL SADGTESALE HGSKTLMATR IVDSIAAFLK SQDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaggcgt gcactgttgt ccgtcaactc accgaggctg ggcattcggt gcgggtcgtc cccaccgagt ccgcgttgcg cttcgtcggt gccgcgacct tcgaggcgct gtccggtaac ccggtgcaca ccggggtgtt caccgacgtc cacgaggtgc agcacgtccg catcggccag caggccgacc tcgtcgtcat cgcacctgcc accgccgatc tcctggcccg cgcggtcgcg gggcgtgccg acgatctgct gaccgccacg ctgctcaccg cacgatgccc cgtgctgttc gcgcctgcca tgcacaccga gatgtggttg cacccggcca ccgtcgacaa cgtggccacg ctgcgccgtc gcggtgcggt ggtgctggaa ccggcgtccg gacgtctcac aggcgccgac agcggcccgg gccggctccc tgaggccgag gagatcacca cactcgcgca gctgctgctg gaacgcgccg acgcgcttcc ctacgacatg gccggggtca aggccctggt gaccgctggt ggtacgcgtg agccgctcga cccggtccgt ttcatcggca accgcagctc cggcaagcag ggctacgccg tcgcgcgtgt gctggcccag cgtggcgccg acgtgacgct catcgcgggc aacacggccg gtctgatcga tcccgcgggc gtcgagatgg tccacatcgg ctcggcgacc cagctgcgcg acgcggtgtc caagcacgca cccgacgcga acgttctggt gatggccgcc gcggtcgccg acttccgtcc tgcgcacgtt gccgccgcga agatcaagaa gggcgcctcg gaacccagct cgatcgatct tgtccgcaac gacgacgtgc tggccggagc ggtccgcgcc agggccgacg gacagttgcc aaacatgcgc gcgatcgtcg gtttcgccgc ggaaaccggg gacgcgaacg gcgacgtgct gttccacgcc agggcgaaac tcgagcgtaa gggctgtgac ctgctggtcg tcaacgccgt gggggagaac cgggcattcg aggtggacca caacgacgga tggttgctga gtgccgacgg caccgagtcc gcactggagc acggctcgaa gacgctcatg gccacccgta tcgtggactc gattgcggcc ttcctgaaga gccaggacgg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for MysmA.01254.a.B3.GE36899
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 18kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMK RIVVGVAGGI AAYKACTVVR QLTEAGHSVR VVPTESALRF VGAATFEALS GNPVHTGVFT DVHEVQHVRI GQQADLVVIA PATADLLARA VAGRADDLLT ATLLTARCPV LFAPAMHTEM WLHPATVDNV ATLRRRGAVV LEPASGRLTG ADSGPGRLPE AEEITTLAQL LLERADALPY DMAG
Validated NT Sequence
atggctcacc accaccacca ccatatgaag cggatcgtcg tcggcgtcgc cggcgggatc gccgcctaca aggcgtgcac tgttgtccgt caactcaccg aggctgggca ttcggtgcgg gtcgtcccca ccgagtccgc gttgcgcttc gtcggtgccg cgaccttcga ggcgctgtcc ggtaacccgg tgcacaccgg ggtgttcacc gacgtccacg aggtgcagca cgtccgcatc ggccagcagg ccgacctcgt cgtcatcgca cctgccaccg ccgatctcct ggcccgcgcg gtcgcggggc gtgccgacga tctgctgacc gccacgctgc tcaccgcacg atgccccgtg ctgttcgcgc ctgccatgca caccgagatg tggttgcacc cggccaccgt cgacaacgtg gccacgctgc gccgtcgcgg tgcggtggtg ctggaaccgg cgtccggacg tctcacaggc gccgacagcg gcccgggccg gctccctgag gccgaggaga tcaccacact cgcgcagctg ctgctggaac gcgccgacgc gcttccctac gacatggccg ggtaagtgag taagat
Expected Protein Sequence
MAHHHHHHKR IVVGVAGGIA AYKACTVVRQ LTEAGHSVRV VPTESALRFV GAATFEALSG NPVHTGVFTD VHEVQHVRIG QQADLVVIAP ATADLLARAV AGRADDLLTA TLLTARCPVL FAPAMHTEMW LHPATVDNVA TLRRRGAVVL EPASGRLTGA DSGPGRLPEA EEITTLAQLL LERADALPYD MAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagcgga tcgtcgtcgg cgtcgccggc gggatcgccg cctacaaggc gtgcactgtt gtccgtcaac tcaccgaggc tgggcattcg gtgcgggtcg tccccaccga gtccgcgttg cgcttcgtcg gtgccgcgac cttcgaggcg ctgtccggta acccggtgca caccggggtg ttcaccgacg tccacgaggt gcagcacgtc cgcatcggcc agcaggccga cctcgtcgtc atcgcacctg ccaccgccga tctcctggcc cgcgcggtcg cggggcgtgc cgacgatctg ctgaccgcca cgctgctcac cgcacgatgc cccgtgctgt tcgcgcctgc catgcacacc gagatgtggt tgcacccggc caccgtcgac aacgtggcca cgctgcgccg tcgcggtgcg gtggtgctgg aaccggcgtc cggacgtctc acaggcgccg acagcggccc gggccggctc cctgaggccg aggagatcac cacactcgcg cagctgctgc tggaacgcgc cgacgcgctt ccctacgaca tggccgggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MysmA.01254.a.B4.GE36900
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDM AGVKALVTAG GTREPLDPVR FIGNRSSGKQ GYAVARVLAQ RGADVTLIAG NTAGLIDPAG VEMVHIGSAT QLRDAVSKHA PDANVLVMAA AVADFRPAHV AAAKIKKGAS EPSSIDLVRN DDVLAGAVRA RADGQLPNMR AIVGFAAETG DANGDVLFHA RAKLERKGCD LLVVNAVGEN RAFEVDHNDG WLLSADGTES ALEHGSKTLM ATRIVDSIAA FLKSQDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacatgg ccggggtcaa ggccctggtg accgctggtg gtacgcgtga gccgctcgac ccggtccgtt tcatcggcaa ccgcagctcc ggcaagcagg gctacgccgt cgcgcgtgtg ctggcccagc gtggcgccga cgtgacgctc atcgcgggca acacggccgg tctgatcgat cccgcgggcg tcgagatggt ccacatcggc tcggcgaccc agctgcgcga cgcggtgtcc aagcacgcac ccgacgcgaa cgttctggtg atggccgccg cggtcgccga cttccgtcct gcgcacgttg ccgccgcgaa gatcaagaag ggcgcctcgg aacccagctc gatcgatctt gtccgcaacg acgacgtgct ggccggagcg gtccgcgcca gggccgacgg acagttgcca aacatgcgcg cgatcgtcgg tttcgccgcg gaaaccgggg acgcgaacgg cgacgtgctg ttccacgcca gggcgaaact cgagcgtaag ggctgtgacc tgctggtcgt caacgccgtg ggggagaacc gggcattcga ggtggaccac aacgacggat ggttgctgag tgccgacggc accgagtccg cactggagca cggctcgaag acgctcatgg ccacccgtat cgtggactcg attgcggcct tcctgaagag ccaggacggg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MysmA.01254.a.B1.PW37336
| PURIFICATION DATe: | 7/29/2013 |
| CONCENTRATION: | 28.5mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 67 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV SARKRIVVGV AGGIAAYKAC TVVRQLTEAG HSVRVVPTES ALRFVGAATF EALSGNPVHT GVFTDVHEVQ HVRIGQQADL VVIAPATADL LARAVAGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPASG RLTGADSGPG RLPEAEEITT LAQLLLERAD ALPYDMAGVK ALVTAGGTRE PLDPVRFIGN RSSGKQGYAV ARVLAQRGAD VTLIAGNTAG LIDPAGVEMV HIGSATQLRD AVSKHAPDAN VLV
Validated NT Sequence
atggctcacc accaccacca ccatatggtg agcgcgcgca agcggatcgt cgtcggcgtc gccggcggga tcgccgccta caaggcgtgc actgttgtcc gtcaactcac cgaggctggg cattcggtgc gggtcgtccc caccgagtcc gcgttgcgct tcgtcggtgc cgcgaccttc gaggcgctgt ccggtaaccc ggtgcacacc ggggtgttca ccgacgtcca cgaggtgcag cacgtccgca tcggccagca ggccgacctc gtcgtcatcg cacctgccac cgccgatctc ctggcccgcg cggtcgcggg gcgtgccgac gatctgctga ccgccacgct gctcaccgca cgatgccccg tgctgttcgc gcctgccatg cacaccgaga tgtggttgca cccggccacc gtcgacaacg tggccacgct gcgccgtcgc ggtgcggtgg tgctggaacc ggcgtccgga cgtctcacag gcgccgacag cggcccgggc cggctccctg aggccgagga gatcaccaca ctcgcgcagc tgctgctgga acgcgccgac gcgcttccct acgacatggc cggggtcaag gccctggtga ccgctggtgg tacgcgtgag ccgctcgacc cggtccgttt catcggcaac cgcagctccg gcaagcaggg ctacgccgtc gcgcgtgtgc tggcccagcg tggcgccgac gtgacgctca tcgcgggcaa cacggccggt ctgatcgatc ccgcgggcgt cgagatggtc cacatcggct cggcgaccca gctgcgcgac gcggtgtcca agcacgcacc cgacgcgaac gttctggtga ng
Expressed Protein Sequence
MAHHHHHHMS ARKRIVVGVA GGIAAYKACT VVRQLTEAGH SVRVVPTESA LRFVGAATFE ALSGNPVHTG VFTDVHEVQH VRIGQQADLV VIAPATADLL ARAVAGRADD LLTATLLTAR CPVLFAPAMH TEMWLHPATV DNVATLRRRG AVVLEPASGR LTGADSGPGR LPEAEEITTL AQLLLERADA LPYDMAGVKA LVTAGGTREP LDPVRFIGNR SSGKQGYAVA RVLAQRGADV TLIAGNTAGL IDPAGVEMVH IGSATQLRDA VSKHAPDANV LVMAAAVADF RPAHVAAAKI KKGASEPSSI DLVRNDDVLA GAVRARADGQ LPNMRAIVGF AAETGDANGD VLFHARAKLE RKGCDLLVVN AVGENRAFEV DHNDGWLLSA DGTESALEHG SKTLMATRIV DSIAAFLKSQ DG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCG CGCGCAAGCG GATCGTCGTC GGCGTCGCCG GCGGGATCGC CGCCTACAAG GCGTGCACTG TTGTCCGTCA ACTCACCGAG GCTGGGCATT CGGTGCGGGT CGTCCCCACC GAGTCCGCGT TGCGCTTCGT CGGTGCCGCG ACCTTCGAGG CGCTGTCCGG TAACCCGGTG CACACCGGGG TGTTCACCGA CGTCCACGAG GTGCAGCACG TCCGCATCGG CCAGCAGGCC GACCTCGTCG TCATCGCACC TGCCACCGCC GATCTCCTGG CCCGCGCGGT CGCGGGGCGT GCCGACGATC TGCTGACCGC CACGCTGCTC ACCGCACGAT GCCCCGTGCT GTTCGCGCCT GCCATGCACA CCGAGATGTG GTTGCACCCG GCCACCGTCG ACAACGTGGC CACGCTGCGC CGTCGCGGTG CGGTGGTGCT GGAACCGGCG TCCGGACGTC TCACAGGCGC CGACAGCGGC CCGGGCCGGC TCCCTGAGGC CGAGGAGATC ACCACACTCG CGCAGCTGCT GCTGGAACGC GCCGACGCGC TTCCCTACGA CATGGCCGGG GTCAAGGCCC TGGTGACCGC TGGTGGTACG CGTGAGCCGC TCGACCCGGT CCGTTTCATC GGCAACCGCA GCTCCGGCAA GCAGGGCTAC GCCGTCGCGC GTGTGCTGGC CCAGCGTGGC GCCGACGTGA CGCTCATCGC GGGCAACACG GCCGGTCTGA TCGATCCCGC GGGCGTCGAG ATGGTCCACA TCGGCTCGGC GACCCAGCTG CGCGACGCGG TGTCCAAGCA CGCACCCGAC GCGAACGTTC TGGTGATGGC CGCCGCGGTC GCCGACTTCC GTCCTGCGCA CGTTGCCGCC GCGAAGATCA AGAAGGGCGC CTCGGAACCC AGCTCGATCG ATCTTGTCCG CAACGACGAC GTGCTGGCCG GAGCGGTCCG CGCCAGGGCC GACGGACAGT TGCCAAACAT GCGCGCGATC GTCGGTTTCG CCGCGGAAAC CGGGGACGCG AACGGCGACG TGCTGTTCCA CGCCAGGGCG AAACTCGAGC GTAAGGGCTG TGACCTGCTG GTCGTCAACG CCGTGGGGGA GAACCGGGCA TTCGAGGTGG ACCACAACGA CGGATGGTTG CTGAGTGCCG ACGGCACCGA GTCCGCACTG GAGCACGGCT CGAAGACGCT CATGGCCACC CGTATCGTGG ACTCGATTGC GGCCTTCCTG AAGAGCCAGG ACGGGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT