MypaA.01254.a

Dfp

CENTER ID: MypaA.01254.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MypaA.01254.a.A1.GE29568 Full length( MypaA.01254.a ) 1 420
MypaA.01254.a.A1.GE37626 Full length( MypaA.01254.a ) 1 420
MypaA.01254.a.B1.GE37625 Full length( MypaA.01254.a ) 1 420
MypaA.01254.a.B2.GE36895 Phosphopantothenoylcysteine decarboxylase 12 196
MypaA.01254.a.B3.GE36896 Phosphopantothenate-cysteine ligase 193 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MypaA.01254.a.B3.PW37337 Phosphopantothenate-cysteine ligase 193 420
MypaA.01254.a.B3.PW37338 Phosphopantothenate-cysteine ligase 193 420
MypaA.01254.a.A1.PW37460 Full length( MypaA.01254.a ) 1 420
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127040
PATRIC ID: fig|262316.17.peg.1184
RefSeq: NP_960059.1
UniProt: Q741G6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYDRNRASTR AARIVVGVSG GIAAYKACTV VRQLSEAGHS VRVIPTESAL RFVGAATFEA LSGQPVHTGV FDDVPEVPHV QLGKQADLVV VAPATADLLA RAVHGRADDL LTATLLTARC PVLFAPAMHT EMWLHPATVD NVATLRRRGA VVLEPAAGRL TGTDSGSGRL PEAEEITTLA QLLLERHDAL PYDLAGCKMV VTAGGTREPV DPVRFIGNRS SGKQGYAVAR VAAQRGAEVT LIAGHTAGLI DPAGVEVVHV SSAEQLGNAV SKHAAEADVL VMAAAVADFR PARVAAAKIK KGVEAPTIEL VRNDDVLAGA VRARAHGELP NLRAIVGFAA ETGDANGDVL FHARAKLRNK GCDLLVVNAV GEGRAFEVDS NDGWLLAADG TESALQHGSK TLMASRIVDA IAAFLRGDGG
NT Sequence
GTGTACGACC GCAATCGGGC TTCCACACGG GCCGCGAGGA TCGTCGTCGG CGTCTCCGGC GGCATCGCCG CCTACAAGGC GTGCACCGTC GTCCGCCAGC TGTCTGAGGC CGGTCATTCG GTCCGGGTCA TCCCCACCGA GTCCGCGCTG CGCTTCGTCG GCGCCGCCAC CTTCGAGGCG CTGTCCGGCC AGCCGGTGCA CACCGGCGTC TTCGACGACG TGCCCGAGGT GCCGCACGTG CAGCTGGGCA AGCAGGCCGA CCTGGTGGTG GTGGCACCGG CCACCGCCGA CCTGCTGGCC CGTGCGGTGC ACGGCCGGGC CGACGACCTG CTGACCGCCA CCCTGCTCAC CGCCCGATGT CCGGTGCTGT TCGCGCCGGC GATGCACACC GAGATGTGGT TGCACCCGGC CACCGTCGAC AACGTGGCCA CGCTGCGCCG CCGCGGGGCG GTGGTGCTCG AGCCCGCCGC CGGGCGGCTC ACCGGCACCG ACAGCGGGTC GGGCCGGCTG CCCGAGGCCG AGGAGATCAC CACCCTGGCC CAGCTGCTGC TGGAACGCCA CGACGCGCTG CCCTACGACC TGGCCGGCTG CAAGATGGTG GTCACCGCCG GCGGCACCCG CGAACCCGTC GACCCGGTGC GCTTCATCGG CAACCGCAGC TCCGGCAAGC AGGGCTACGC GGTGGCCCGG GTGGCCGCCC AGCGCGGCGC CGAGGTCACG CTGATCGCCG GGCACACCGC CGGACTGATC GACCCCGCCG GGGTCGAGGT GGTGCACGTC AGCTCGGCGG AGCAGCTGGG CAACGCGGTG TCCAAGCACG CGGCCGAGGC CGACGTGCTG GTGATGGCCG CCGCCGTCGC CGACTTCCGG CCCGCCCGGG TTGCCGCCGC CAAGATCAAA AAGGGTGTCG AGGCACCGAC GATCGAGTTG GTCCGCAACG ACGACGTGCT GGCCGGCGCG GTGCGGGCGC GCGCCCACGG CGAGCTGCCC AACCTGCGGG CCATCGTGGG ATTCGCCGCC GAGACCGGAG ACGCCAACGG GGACGTGCTG TTTCACGCCC GCGCCAAGCT GCGCAACAAG GGCTGTGACC TGTTGGTGGT CAACGCGGTG GGCGAGGGCC GGGCCTTCGA GGTGGACAGC AACGACGGCT GGCTGCTGGC GGCCGACGGG ACCGAGTCGG CGCTGCAGCA CGGCTCCAAG ACGCTGATGG CCAGTCGTAT CGTGGATGCG ATCGCCGCCT TCCTGCGCGG GGACGGCGGA
Details for MypaA.01254.a.A1.GE29568
HARVESTED ON: 6/15/2010
SEQUENCED ON: 3/3/2015
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDRNRAS TRAARIVVGV SGGIAAYKAC TVVRQLSEAG HSVRVIPTES ALRFVGAATF EALSGQPVHT GVFDDVPEVP HVQLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPAAG RLTGTDSGSG RLPEAEEITT LAQLLLERHD ALPYDLAGCK MVVTAGGTRE PVDPVRFIGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTAG LIDPAGVEVV HVSSAEQLGN AVSKHAAEAD VLVMAAAVAD FRPARVAAAK IKKGVEAPTI ELVRNDDVLA GAVRARAHGE LPNLRAIVGF AAETGDANGD VLFHARAKLR NKGCDLLVVN AVGEGRAFEV DSNDGWLLAA DGTESALQHG SKTLMASRIV DAIAAFLRGD GGN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt acgaccgcaa tcgggcttcc acacgggccg cgaggatcgt cgtcggcgtc tccggcggca tcgccgccta caaggcgtgc accgtcgtcc gccagctgtc tgaggccggt cattcggtcc gggtcatccc caccgagtcc gcgctgcgct tcgtcggcgc cgccaccttc gaggcgctgt ccggccagcc ggtgcacacc ggcgtcttcg acgacgtgcc cgaggtgccg cacgtgcagc tgggcaagca ggccgacctg gtggtggtgg caccggccac cgccgacctg ctggcccgtg cggtgcacgg ccgggccgac gacctgctga ccgccaccct gctcaccgcc cgatgtccgg tgctgttcgc gccggcgatg cacaccgaga tgtggttgca cccggccacc gtcgacaacg tggccacgct gcgccgccgc ggggcggtgg tgctcgagcc cgccgccggg cggctcaccg gcaccgacag cgggtcgggc cggctgcccg aggccgagga gatcaccacc ctggcccagc tgctgctgga acgccacgac gcgctgccct acgacctggc cggctgcaag atggtggtca ccgccggcgg cacccgcgaa cccgtcgacc cggtgcgctt catcggcaac cgcagctccg gcaagcaggg ctacgcggtg gcccgggtgg ccgcccagcg cggcgccgag gtcacgctga tcgccgggca caccgccgga ctgatcgacc ccgccggggt cgaggtggtg cacgtcagct cggcggagca gctgggcaac gcggtgtcca agcacgcggc cgaggccgac gtgctggtga tggccgccgc cgtcgccgac ttccggcccg cccgggttgc cgccgccaag atcaaaaagg gtgtcgaggc accgacgatc gagttggtcc gcaacgacga cgtgctggcc ggcgcggtgc gggcgcgcgc ccacggcgag ctgcccaacc tgcgggccat cgtgggattc gccgccgaga ccggagacgc caacggggac gtgctgtttc acgcccgcgc caagctgcgc aacaagggct gtgacctgtt ggtggtcaac gcggtgggcg agggccgggc cttcgaggtg gacagcaacg acggctggct gctggcggcc gacgggaccg agtcggcgct gcagcacggc tccaagacgc tgatggccag tcgtatcgtg gatgcgatcg ccgccttcct gcgcggggac ggcggtaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDRNRAS TRAARIVVGV SGGIAAYKAC TVVRQLSEAG HSVRVIPTES ALRFVGAATF EALSGQPVHT GVFDDVPEVP HVQLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPAAG RLTGTDSGSG RLPEAEEITT LAQLLLERHD ALPYDLAGCK MVVTAGGTRE PVDPVRFIGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTAG LIDPAGVEVV HVSSAEQLGN AVSKHAAEAD VLVMAAAVAD FRPARVAAAK IKKGVEAPTI ELVRNDDVLA GAVRARAHGE LPNLRAIVGF AAETGDANGD VLFHARAKLR NKGCDLLVVN AVGEGRAFEV DSNDGWLLAA DGTESALQHG SKTLMASRIV DAIAAFLRGD GG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtacgac cgcaatcggg cttccacacg ggccgcgagg atcgtcgtcg gcgtctccgg cggcatcgcc gcctacaagg cgtgcaccgt cgtccgccag ctgtctgagg ccggtcattc ggtccgggtc atccccaccg agtccgcgct gcgcttcgtc ggcgccgcca ccttcgaggc gctgtccggc cagccggtgc acaccggcgt cttcgacgac gtgcccgagg tgccgcacgt gcagctgggc aagcaggccg acctggtggt ggtggcaccg gccaccgccg acctgctggc ccgtgcggtg cacggccggg ccgacgacct gctgaccgcc accctgctca ccgcccgatg tccggtgctg ttcgcgccgg cgatgcacac cgagatgtgg ttgcacccgg ccaccgtcga caacgtggcc acgctgcgcc gccgcggggc ggtggtgctc gagcccgccg ccgggcggct caccggcacc gacagcgggt cgggccggct gcccgaggcc gaggagatca ccaccctggc ccagctgctg ctggaacgcc acgacgcgct gccctacgac ctggccggct gcaagatggt ggtcaccgcc ggcggcaccc gcgaacccgt cgacccggtg cgcttcatcg gcaaccgcag ctccggcaag cagggctacg cggtggcccg ggtggccgcc cagcgcggcg ccgaggtcac gctgatcgcc gggcacaccg ccggactgat cgaccccgcc ggggtcgagg tggtgcacgt cagctcggcg gagcagctgg gcaacgcggt gtccaagcac gcggccgagg ccgacgtgct ggtgatggcc gccgccgtcg ccgacttccg gcccgcccgg gttgccgccg ccaagatcaa aaagggtgtc gaggcaccga cgatcgagtt ggtccgcaac gacgacgtgc tggccggcgc ggtgcgggcg cgcgcccacg gcgagctgcc caacctgcgg gccatcgtgg gattcgccgc cgagaccgga gacgccaacg gggacgtgct gtttcacgcc cgcgccaagc tgcgcaacaa gggctgtgac ctgttggtgg tcaacgcggt gggcgagggc cgggccttcg aggtggacag caacgacggc tggctgctgg cggccgacgg gaccgagtcg gcgctgcagc acggctccaa gacgctgatg gccagtcgta tcgtggatgc gatcgccgcc ttcctgcgcg gggacggcgg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MypaA.01254.a.A1.GE37626
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 46kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDRNRAS TRAARIVVGV SGGIAAYKAC TVVRQLSEAG HSVRVIPTES ALRFVGAATF EALSGQPVHT GVFDDVPEVP HVQLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPAAG RLTGTDSGSG RLPEAEEITT LAQLLLERHD ALPYDLAGCK MVVTAGGTRE PVDPVRFIGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTAG LIDPAGVEVV HVSSAEQLGN AVSKHAAEAD VLVMAAAVAD FRPARVAAAK IKKGVEAPTI ELVRNDDVLA GAVRARAHGE LPNLRAIVGF AAETGDANGD VLFHARAKLR NKGCDLLVVN AVGEGRAFEV DSNDGWLLAA DGTESALQHG SKTLMASRIV DAIAAFLRGD GGN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt acgaccgcaa tcgggcttcc acacgggccg cgaggatcgt cgtcggcgtc tccggcggca tcgccgccta caaggcgtgc accgtcgtcc gccagctgtc tgaggccggt cattcggtcc gggtcatccc caccgagtcc gcgctgcgct tcgtcggcgc cgccaccttc gaggcgctgt ccggccagcc ggtgcacacc ggcgtcttcg acgacgtgcc cgaggtgccg cacgtgcagc tgggcaagca ggccgacctg gtggtggtgg caccggccac cgccgacctg ctggcccgtg cggtgcacgg ccgggccgac gacctgctga ccgccaccct gctcaccgcc cgatgtccgg tgctgttcgc gccggcgatg cacaccgaga tgtggttgca cccggccacc gtcgacaacg tggccacgct gcgccgccgc ggggcggtgg tgctcgagcc cgccgccggg cggctcaccg gcaccgacag cgggtcgggc cggctgcccg aggccgagga gatcaccacc ctggcccagc tgctgctgga acgccacgac gcgctgccct acgacctggc cggctgcaag atggtggtca ccgccggcgg cacccgcgaa cccgtcgacc cggtgcgctt catcggcaac cgcagctccg gcaagcaggg ctacgcggtg gcccgggtgg ccgcccagcg cggcgccgag gtcacgctga tcgccgggca caccgccgga ctgatcgacc ccgccggggt cgaggtggtg cacgtcagct cggcggagca gctgggcaac gcggtgtcca agcacgcggc cgaggccgac gtgctggtga tggccgccgc cgtcgccgac ttccggcccg cccgggttgc cgccgccaag atcaaaaagg gtgtcgaggc accgacgatc gagttggtcc gcaacgacga cgtgctggcc ggcgcggtgc gggcgcgcgc ccacggcgag ctgcccaacc tgcgggccat cgtgggattc gccgccgaga ccggagacgc caacggggac gtgctgtttc acgcccgcgc caagctgcgc aacaagggct gtgacctgtt ggtggtcaac gcggtgggcg agggccgggc cttcgaggtg gacagcaacg acggctggct gctggcggcc gacgggaccg agtcggcgct gcagcacggc tccaagacgc tgatggccag tcgtatcgtg gatgcgatcg ccgccttcct gcgcggggac ggcggtaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDRNRAS TRAARIVVGV SGGIAAYKAC TVVRQLSEAG HSVRVIPTES ALRFVGAATF EALSGQPVHT GVFDDVPEVP HVQLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPAAG RLTGTDSGSG RLPEAEEITT LAQLLLERHD ALPYDLAGCK MVVTAGGTRE PVDPVRFIGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTAG LIDPAGVEVV HVSSAEQLGN AVSKHAAEAD VLVMAAAVAD FRPARVAAAK IKKGVEAPTI ELVRNDDVLA GAVRARAHGE LPNLRAIVGF AAETGDANGD VLFHARAKLR NKGCDLLVVN AVGEGRAFEV DSNDGWLLAA DGTESALQHG SKTLMASRIV DAIAAFLRGD GG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtacgac cgcaatcggg cttccacacg ggccgcgagg atcgtcgtcg gcgtctccgg cggcatcgcc gcctacaagg cgtgcaccgt cgtccgccag ctgtctgagg ccggtcattc ggtccgggtc atccccaccg agtccgcgct gcgcttcgtc ggcgccgcca ccttcgaggc gctgtccggc cagccggtgc acaccggcgt cttcgacgac gtgcccgagg tgccgcacgt gcagctgggc aagcaggccg acctggtggt ggtggcaccg gccaccgccg acctgctggc ccgtgcggtg cacggccggg ccgacgacct gctgaccgcc accctgctca ccgcccgatg tccggtgctg ttcgcgccgg cgatgcacac cgagatgtgg ttgcacccgg ccaccgtcga caacgtggcc acgctgcgcc gccgcggggc ggtggtgctc gagcccgccg ccgggcggct caccggcacc gacagcgggt cgggccggct gcccgaggcc gaggagatca ccaccctggc ccagctgctg ctggaacgcc acgacgcgct gccctacgac ctggccggct gcaagatggt ggtcaccgcc ggcggcaccc gcgaacccgt cgacccggtg cgcttcatcg gcaaccgcag ctccggcaag cagggctacg cggtggcccg ggtggccgcc cagcgcggcg ccgaggtcac gctgatcgcc gggcacaccg ccggactgat cgaccccgcc ggggtcgagg tggtgcacgt cagctcggcg gagcagctgg gcaacgcggt gtccaagcac gcggccgagg ccgacgtgct ggtgatggcc gccgccgtcg ccgacttccg gcccgcccgg gttgccgccg ccaagatcaa aaagggtgtc gaggcaccga cgatcgagtt ggtccgcaac gacgacgtgc tggccggcgc ggtgcgggcg cgcgcccacg gcgagctgcc caacctgcgg gccatcgtgg gattcgccgc cgagaccgga gacgccaacg gggacgtgct gtttcacgcc cgcgccaagc tgcgcaacaa gggctgtgac ctgttggtgg tcaacgcggt gggcgagggc cgggccttcg aggtggacag caacgacggc tggctgctgg cggccgacgg gaccgagtcg gcgctgcagc acggctccaa gacgctgatg gccagtcgta tcgtggatgc gatcgccgcc ttcctgcgcg gggacggcgg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MypaA.01254.a.B1.GE37625
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 66
Validated AA Sequence
MHTEMWLHPA TVDNVATLRR RGAVVLEPAA GRLTGTDSGS GRLPEAEEIT TLAQLLLERH DALPYDLAGC KMVVTAGGTR EPVDPVRFIG NRSSGKQGYA VARVAAQRGA EVTLIAGHTA GLIDPAGVEV VHVSSAEQLG NAVSKHAAEA DVLVMAAAVA DFRPARVAAA KIKKGVEAPT IELVRNDDVL AGAVRARAHG ELPNLRAIVG FAAETGDANG DVLFHARAKL RNKGCDLLVV NAVGEGRAFE VDSNDGWLLA ADGTESALQH GSKTLMASRI VDA
Validated NT Sequence
tcgcatccac gatacgactg gccatcagcg tcttggagcc gtgctgcagc gccgactcgg tcccgtcggc cgccagcagc cagccgtcgt tgctgtccac ctcgaaggcc cggccctcgc ccaccgcgtt gaccaccaac aggtcacagc ccttgttgcg cagcttggcg cgggcgtgaa acagcacgtc cccgttggcg tctccggtct cggcggcgaa tcccacgatg gcccgcaggt tgggcagctc gccgtgggcg cgcgcccgca ccgcgccggc cagcacgtcg tcgttgcgga ccaactcgat cgtcggtgcc tcgacaccct ttttgatctt ggcggcggca acccgggcgg gccggaagtc ggcgacggcg gcggccatca ccagcacgtc ggcctcggcc gcgtgcttgg acaccgcgtt gcccagctgc tccgccgagc tgacgtgcac cacctcgacc ccggcggggt cgatcagtcc ggcggtgtgc ccggcgatca gcgtgacctc ggcgccgcgc tgggcggcca cccgggccac cgcgtagccc tgcttgccgg agctgcggtt gccgatgaag cgcaccgggt cgacgggttc gcgggtgccg ccggcggtga ccaccatctt gcagccggcc aggtcgtagg gcagcgcgtc gtggcgttcc agcagcagct gggccagggt ggtgatctcc tcggcctcgg gcagccggcc cgacccgctg tcggtgccgg tgagccgccc ggcggcgggc tcgagcacca ccgccccgcg gcggcgcagc gtggccacgt tgtcgacggt ggccgggtgc aaccacatct cggtgtgcat cgccggcgcg aacagcaccg gacatcgggc ggtgagcagg gtggcggtca gcaggtcgtc ggcccggccg tgcaccgcac gggccagcag gtcggcggtg gccggtgcca ccaccaccag gtcggcctgc ttgcccagct gcacgtgcgg cacctcgggc acgtcgtcga agacgccggt gtgcaccggc tggccggaca gcgcctcgaa ggtggcggcg ccgacgaagc gcagcgcgga ctcggtgggg atgacccgga ccgaatgacc ggcctcagac agctggcgga cgacggtgca cgccttgtag gcggcgatgc cgccggagac gccgacgacg atcctcgcgg cccgtgtgga agcccgattg cg
Expected Protein Sequence
MAHHHHHHMY DRNRASTRAA RIVVGVSGGI AAYKACTVVR QLSEAGHSVR VIPTESALRF VGAATFEALS GQPVHTGVFD DVPEVPHVQL GKQADLVVVA PATADLLARA VHGRADDLLT ATLLTARCPV LFAPAMHTEM WLHPATVDNV ATLRRRGAVV LEPAAGRLTG TDSGSGRLPE AEEITTLAQL LLERHDALPY DLAGCKMVVT AGGTREPVDP VRFIGNRSSG KQGYAVARVA AQRGAEVTLI AGHTAGLIDP AGVEVVHVSS AEQLGNAVSK HAAEADVLVM AAAVADFRPA RVAAAKIKKG VEAPTIELVR NDDVLAGAVR ARAHGELPNL RAIVGFAAET GDANGDVLFH ARAKLRNKGC DLLVVNAVGE GRAFEVDSND GWLLAADGTE SALQHGSKTL MASRIVDAIA AFLRGDGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtacg accgcaatcg ggcttccaca cgggccgcga ggatcgtcgt cggcgtctcc ggcggcatcg ccgcctacaa ggcgtgcacc gtcgtccgcc agctgtctga ggccggtcat tcggtccggg tcatccccac cgagtccgcg ctgcgcttcg tcggcgccgc caccttcgag gcgctgtccg gccagccggt gcacaccggc gtcttcgacg acgtgcccga ggtgccgcac gtgcagctgg gcaagcaggc cgacctggtg gtggtggcac cggccaccgc cgacctgctg gcccgtgcgg tgcacggccg ggccgacgac ctgctgaccg ccaccctgct caccgcccga tgtccggtgc tgttcgcgcc ggcgatgcac accgagatgt ggttgcaccc ggccaccgtc gacaacgtgg ccacgctgcg ccgccgcggg gcggtggtgc tcgagcccgc cgccgggcgg ctcaccggca ccgacagcgg gtcgggccgg ctgcccgagg ccgaggagat caccaccctg gcccagctgc tgctggaacg ccacgacgcg ctgccctacg acctggccgg ctgcaagatg gtggtcaccg ccggcggcac ccgcgaaccc gtcgacccgg tgcgcttcat cggcaaccgc agctccggca agcagggcta cgcggtggcc cgggtggccg cccagcgcgg cgccgaggtc acgctgatcg ccgggcacac cgccggactg atcgaccccg ccggggtcga ggtggtgcac gtcagctcgg cggagcagct gggcaacgcg gtgtccaagc acgcggccga ggccgacgtg ctggtgatgg ccgccgccgt cgccgacttc cggcccgccc gggttgccgc cgccaagatc aaaaagggtg tcgaggcacc gacgatcgag ttggtccgca acgacgacgt gctggccggc gcggtgcggg cgcgcgccca cggcgagctg cccaacctgc gggccatcgt gggattcgcc gccgagaccg gagacgccaa cggggacgtg ctgtttcacg cccgcgccaa gctgcgcaac aagggctgtg acctgttggt ggtcaacgcg gtgggcgagg gccgggcctt cgaggtggac agcaacgacg gctggctgct ggcggccgac gggaccgagt cggcgctgca gcacggctcc aagacgctga tggccagtcg tatcgtggat gcgatcgccg ccttcctgcg cggggacggc ggatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MypaA.01254.a.B2.GE36895
HARVESTED ON: 5/1/2013
SEQUENCED ON: 3/3/2015
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RIVVGVSGGI AAYKACTVVR QLSEAGHSVR VIPTESALRF VGAATFEALS GQPVHTGVFD DVPEVPHVQL GKQADLVVVA PATADLLARA VHGRADDLLT ATLLTARCPV LFAPAMHTEM WLHPATVDNV ATLRRRGAVV LEPAAGRLTG TDSGSGRLPE AEEITTLAQL LLERHDALPY DLAG
Validated NT Sequence
atggctcacc accaccacca ccatatggcg aggatcgtcg tcggcgtctc cggcggcatc gccgcctaca aggcgtgcac cgtcgtccgc cagctgtctg aggccggtca ttcggtccgg gtcatcccca ccgagtccgc gctgcgcttc gtcggcgccg ccaccttcga ggcgctgtcc ggccagccgg tgcacaccgg cgtcttcgac gacgtgcccg aggtgccgca cgtgcagctg ggcaagcagg ccgacctggt ggtggtggca ccggccaccg ccgacctgct ggcccgtgcg gtgcacggcc gggccgacga cctgctgacc gccaccctgc tcaccgcccg atgtccggtg ctgttcgcgc cggcgatgca caccgagatg tggttgcacc cggccaccgt cgacaacgtg gccacgctgc gccgccgcgg ggcggtggtg ctcgagcccg ccgccgggcg gctcaccggc accgacagcg ggtcgggccg gctgcccgag gccgaggaga tcaccaccct ggcccagctg ctgctggaac gccacgacgc gctgccctac gacctggccg gctaagtgag taagat
Expected Protein Sequence
MAHHHHHHAR IVVGVSGGIA AYKACTVVRQ LSEAGHSVRV IPTESALRFV GAATFEALSG QPVHTGVFDD VPEVPHVQLG KQADLVVVAP ATADLLARAV HGRADDLLTA TLLTARCPVL FAPAMHTEMW LHPATVDNVA TLRRRGAVVL EPAAGRLTGT DSGSGRLPEA EEITTLAQLL LERHDALPYD LAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgagga tcgtcgtcgg cgtctccggc ggcatcgccg cctacaaggc gtgcaccgtc gtccgccagc tgtctgaggc cggtcattcg gtccgggtca tccccaccga gtccgcgctg cgcttcgtcg gcgccgccac cttcgaggcg ctgtccggcc agccggtgca caccggcgtc ttcgacgacg tgcccgaggt gccgcacgtg cagctgggca agcaggccga cctggtggtg gtggcaccgg ccaccgccga cctgctggcc cgtgcggtgc acggccgggc cgacgacctg ctgaccgcca ccctgctcac cgcccgatgt ccggtgctgt tcgcgccggc gatgcacacc gagatgtggt tgcacccggc caccgtcgac aacgtggcca cgctgcgccg ccgcggggcg gtggtgctcg agcccgccgc cgggcggctc accggcaccg acagcgggtc gggccggctg cccgaggccg aggagatcac caccctggcc cagctgctgc tggaacgcca cgacgcgctg ccctacgacc tggccggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MypaA.01254.a.B3.GE36896
HARVESTED ON: 5/1/2013
SEQUENCED ON: 3/3/2015
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LAGCKMVVTA GGTREPVDPV RFIGNRSSGK QGYAVARVAA QRGAEVTLIA GHTAGLIDPA GVEVVHVSSA EQLGNAVSKH AAEADVLVMA AAVADFRPAR VAAAKIKKGV EAPTIELVRN DDVLAGAVRA RAHGELPNLR AIVGFAAETG DANGDVLFHA RAKLRNKGCD LLVVNAVGEG RAFEVDSNDG WLLAADGTES ALQHGSKTLM ASRIVDAIAA FLRGDGG
Validated NT Sequence
atggctcacc accaccacca ccatatggac ctggccggct gcaagatggt ggtcaccgcc ggcggcaccc gcgaacccgt cgacccggtg cgcttcatcg gcaaccgcag ctccggcaag cagggctacg cggtggcccg ggtggccgcc cagcgcggcg ccgaggtcac gctgatcgcc gggcacaccg ccggactgat cgaccccgcc ggggtcgagg tggtgcacgt cagctcggcg gagcagctgg gcaacgcggt gtccaagcac gcggccgagg ccgacgtgct ggtgatggcc gccgccgtcg ccgacttccg gcccgcccgg gttgccgccg ccaagatcaa aaagggtgtc gaggcaccga cgatcgagtt ggtccgcaac gacgacgtgc tggccggcgc ggtgcgggcg cgcgcccacg gcgagctgcc caacctgcgg gccatcgtgg gattcgccgc cgagaccgga gacgccaacg gggacgtgct gtttcacgcc cgcgccaagc tgcgcaacaa gggctgtgac ctgttggtgg tcaacgcggt gggcgagggc cgggccttcg aggtggacag caacgacggc tggctgctgg cggccgacgg gaccgagtcg gcgctgcagc acggctccaa gacgctgatg gccagtcgta tcgtggatgc gatcgccgcc ttcctgcgcg gggacggcgg ataagtgagt aagat
Expected Protein Sequence
MAHHHHHHDL AGCKMVVTAG GTREPVDPVR FIGNRSSGKQ GYAVARVAAQ RGAEVTLIAG HTAGLIDPAG VEVVHVSSAE QLGNAVSKHA AEADVLVMAA AVADFRPARV AAAKIKKGVE APTIELVRND DVLAGAVRAR AHGELPNLRA IVGFAAETGD ANGDVLFHAR AKLRNKGCDL LVVNAVGEGR AFEVDSNDGW LLAADGTESA LQHGSKTLMA SRIVDAIAAF LRGDGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacctgg ccggctgcaa gatggtggtc accgccggcg gcacccgcga acccgtcgac ccggtgcgct tcatcggcaa ccgcagctcc ggcaagcagg gctacgcggt ggcccgggtg gccgcccagc gcggcgccga ggtcacgctg atcgccgggc acaccgccgg actgatcgac cccgccgggg tcgaggtggt gcacgtcagc tcggcggagc agctgggcaa cgcggtgtcc aagcacgcgg ccgaggccga cgtgctggtg atggccgccg ccgtcgccga cttccggccc gcccgggttg ccgccgccaa gatcaaaaag ggtgtcgagg caccgacgat cgagttggtc cgcaacgacg acgtgctggc cggcgcggtg cgggcgcgcg cccacggcga gctgcccaac ctgcgggcca tcgtgggatt cgccgccgag accggagacg ccaacgggga cgtgctgttt cacgcccgcg ccaagctgcg caacaagggc tgtgacctgt tggtggtcaa cgcggtgggc gagggccggg ccttcgaggt ggacagcaac gacggctggc tgctggcggc cgacgggacc gagtcggcgc tgcagcacgg ctccaagacg ctgatggcca gtcgtatcgt ggatgcgatc gccgccttcc tgcgcgggga cggcggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MypaA.01254.a.B3.PW37337
PURIFICATION DATe: 7/29/2013
CONCENTRATION: 23.1mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LAGCKMVVTA GGTREPVDPV RFIGNRSSGK QGYAVARVAA QRGAEVTLIA GHTAGLIDPA GVEVVHVSSA EQLGNAVSKH AAEADVLVMA AAVADFRPAR VAAAKIKKGV EAPTIELVRN DDVLAGAVRA RAHGELPNLR AIVGFAAETG DANGDVLFHA RAKLRNKGCD LLVVNAVGEG RAFEVDSNDG WLLAADGTES ALQHGSKTLM ASRIVDAIAA FLRGDGG
Validated NT Sequence
atggctcacc accaccacca ccatatggac ctggccggct gcaagatggt ggtcaccgcc ggcggcaccc gcgaacccgt cgacccggtg cgcttcatcg gcaaccgcag ctccggcaag cagggctacg cggtggcccg ggtggccgcc cagcgcggcg ccgaggtcac gctgatcgcc gggcacaccg ccggactgat cgaccccgcc ggggtcgagg tggtgcacgt cagctcggcg gagcagctgg gcaacgcggt gtccaagcac gcggccgagg ccgacgtgct ggtgatggcc gccgccgtcg ccgacttccg gcccgcccgg gttgccgccg ccaagatcaa aaagggtgtc gaggcaccga cgatcgagtt ggtccgcaac gacgacgtgc tggccggcgc ggtgcgggcg cgcgcccacg gcgagctgcc caacctgcgg gccatcgtgg gattcgccgc cgagaccgga gacgccaacg gggacgtgct gtttcacgcc cgcgccaagc tgcgcaacaa gggctgtgac ctgttggtgg tcaacgcggt gggcgagggc cgggccttcg aggtggacag caacgacggc tggctgctgg cggccgacgg gaccgagtcg gcgctgcagc acggctccaa gacgctgatg gccagtcgta tcgtggatgc gatcgccgcc ttcctgcgcg gggacggcgg ataagtgagt aagat
Expressed Protein Sequence
MAHHHHHHDL AGCKMVVTAG GTREPVDPVR FIGNRSSGKQ GYAVARVAAQ RGAEVTLIAG HTAGLIDPAG VEVVHVSSAE QLGNAVSKHA AEADVLVMAA AVADFRPARV AAAKIKKGVE APTIELVRND DVLAGAVRAR AHGELPNLRA IVGFAAETGD ANGDVLFHAR AKLRNKGCDL LVVNAVGEGR AFEVDSNDGW LLAADGTESA LQHGSKTLMA SRIVDAIAAF LRGDGG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACCTGG CCGGCTGCAA GATGGTGGTC ACCGCCGGCG GCACCCGCGA ACCCGTCGAC CCGGTGCGCT TCATCGGCAA CCGCAGCTCC GGCAAGCAGG GCTACGCGGT GGCCCGGGTG GCCGCCCAGC GCGGCGCCGA GGTCACGCTG ATCGCCGGGC ACACCGCCGG ACTGATCGAC CCCGCCGGGG TCGAGGTGGT GCACGTCAGC TCGGCGGAGC AGCTGGGCAA CGCGGTGTCC AAGCACGCGG CCGAGGCCGA CGTGCTGGTG ATGGCCGCCG CCGTCGCCGA CTTCCGGCCC GCCCGGGTTG CCGCCGCCAA GATCAAAAAG GGTGTCGAGG CACCGACGAT CGAGTTGGTC CGCAACGACG ACGTGCTGGC CGGCGCGGTG CGGGCGCGCG CCCACGGCGA GCTGCCCAAC CTGCGGGCCA TCGTGGGATT CGCCGCCGAG ACCGGAGACG CCAACGGGGA CGTGCTGTTT CACGCCCGCG CCAAGCTGCG CAACAAGGGC TGTGACCTGT TGGTGGTCAA CGCGGTGGGC GAGGGCCGGG CCTTCGAGGT GGACAGCAAC GACGGCTGGC TGCTGGCGGC CGACGGGACC GAGTCGGCGC TGCAGCACGG CTCCAAGACG CTGATGGCCA GTCGTATCGT GGATGCGATC GCCGCCTTCC TGCGCGGGGA CGGCGGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for MypaA.01254.a.B3.PW37338
PURIFICATION DATe: 7/29/2013
CONCENTRATION: 14.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LAGCKMVVTA GGTREPVDPV RFIGNRSSGK QGYAVARVAA QRGAEVTLIA GHTAGLIDPA GVEVVHVSSA EQLGNAVSKH AAEADVLVMA AAVADFRPAR VAAAKIKKGV EAPTIELVRN DDVLAGAVRA RAHGELPNLR AIVGFAAETG DANGDVLFHA RAKLRNKGCD LLVVNAVGEG RAFEVDSNDG WLLAADGTES ALQHGSKTLM ASRIVDAIAA FLRGDGG
Validated NT Sequence
atggctcacc accaccacca ccatatggac ctggccggct gcaagatggt ggtcaccgcc ggcggcaccc gcgaacccgt cgacccggtg cgcttcatcg gcaaccgcag ctccggcaag cagggctacg cggtggcccg ggtggccgcc cagcgcggcg ccgaggtcac gctgatcgcc gggcacaccg ccggactgat cgaccccgcc ggggtcgagg tggtgcacgt cagctcggcg gagcagctgg gcaacgcggt gtccaagcac gcggccgagg ccgacgtgct ggtgatggcc gccgccgtcg ccgacttccg gcccgcccgg gttgccgccg ccaagatcaa aaagggtgtc gaggcaccga cgatcgagtt ggtccgcaac gacgacgtgc tggccggcgc ggtgcgggcg cgcgcccacg gcgagctgcc caacctgcgg gccatcgtgg gattcgccgc cgagaccgga gacgccaacg gggacgtgct gtttcacgcc cgcgccaagc tgcgcaacaa gggctgtgac ctgttggtgg tcaacgcggt gggcgagggc cgggccttcg aggtggacag caacgacggc tggctgctgg cggccgacgg gaccgagtcg gcgctgcagc acggctccaa gacgctgatg gccagtcgta tcgtggatgc gatcgccgcc ttcctgcgcg gggacggcgg ataagtgagt aagat
Expressed Protein Sequence
MAHHHHHHDL AGCKMVVTAG GTREPVDPVR FIGNRSSGKQ GYAVARVAAQ RGAEVTLIAG HTAGLIDPAG VEVVHVSSAE QLGNAVSKHA AEADVLVMAA AVADFRPARV AAAKIKKGVE APTIELVRND DVLAGAVRAR AHGELPNLRA IVGFAAETGD ANGDVLFHAR AKLRNKGCDL LVVNAVGEGR AFEVDSNDGW LLAADGTESA LQHGSKTLMA SRIVDAIAAF LRGDGG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACCTGG CCGGCTGCAA GATGGTGGTC ACCGCCGGCG GCACCCGCGA ACCCGTCGAC CCGGTGCGCT TCATCGGCAA CCGCAGCTCC GGCAAGCAGG GCTACGCGGT GGCCCGGGTG GCCGCCCAGC GCGGCGCCGA GGTCACGCTG ATCGCCGGGC ACACCGCCGG ACTGATCGAC CCCGCCGGGG TCGAGGTGGT GCACGTCAGC TCGGCGGAGC AGCTGGGCAA CGCGGTGTCC AAGCACGCGG CCGAGGCCGA CGTGCTGGTG ATGGCCGCCG CCGTCGCCGA CTTCCGGCCC GCCCGGGTTG CCGCCGCCAA GATCAAAAAG GGTGTCGAGG CACCGACGAT CGAGTTGGTC CGCAACGACG ACGTGCTGGC CGGCGCGGTG CGGGCGCGCG CCCACGGCGA GCTGCCCAAC CTGCGGGCCA TCGTGGGATT CGCCGCCGAG ACCGGAGACG CCAACGGGGA CGTGCTGTTT CACGCCCGCG CCAAGCTGCG CAACAAGGGC TGTGACCTGT TGGTGGTCAA CGCGGTGGGC GAGGGCCGGG CCTTCGAGGT GGACAGCAAC GACGGCTGGC TGCTGGCGGC CGACGGGACC GAGTCGGCGC TGCAGCACGG CTCCAAGACG CTGATGGCCA GTCGTATCGT GGATGCGATC GCCGCCTTCC TGCGCGGGGA CGGCGGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for MypaA.01254.a.A1.PW37460
PURIFICATION DATe: 3/11/2014
CONCENTRATION: 19mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDRNRAS TRAARIVVGV SGGIAAYKAC TVVRQLSEAG HSVRVIPTES ALRFVGAATF EALSGQPVHT GVFDDVPEVP HVQLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPAAG RLTGTDSGSG RLPEAEEITT LAQLLLERHD ALPYDLAGCK MVVTAGGTRE PVDPVRFIGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTAG LIDPAGVEVV HVSSAEQLGN AVSKHAAEAD VLVMAAAVAD FRPARVAAAK IKKGVEAPTI ELVRNDDVLA GAVRARAHGE LPNLRAIVGF AAETGDANGD VLFHARAKLR NKGCDLLVVN AVGEGRAFEV DSNDGWLLAA DGTESALQHG SKTLMASRIV DAIAAFLRGD GGN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt acgaccgcaa tcgggcttcc acacgggccg cgaggatcgt cgtcggcgtc tccggcggca tcgccgccta caaggcgtgc accgtcgtcc gccagctgtc tgaggccggt cattcggtcc gggtcatccc caccgagtcc gcgctgcgct tcgtcggcgc cgccaccttc gaggcgctgt ccggccagcc ggtgcacacc ggcgtcttcg acgacgtgcc cgaggtgccg cacgtgcagc tgggcaagca ggccgacctg gtggtggtgg caccggccac cgccgacctg ctggcccgtg cggtgcacgg ccgggccgac gacctgctga ccgccaccct gctcaccgcc cgatgtccgg tgctgttcgc gccggcgatg cacaccgaga tgtggttgca cccggccacc gtcgacaacg tggccacgct gcgccgccgc ggggcggtgg tgctcgagcc cgccgccggg cggctcaccg gcaccgacag cgggtcgggc cggctgcccg aggccgagga gatcaccacc ctggcccagc tgctgctgga acgccacgac gcgctgccct acgacctggc cggctgcaag atggtggtca ccgccggcgg cacccgcgaa cccgtcgacc cggtgcgctt catcggcaac cgcagctccg gcaagcaggg ctacgcggtg gcccgggtgg ccgcccagcg cggcgccgag gtcacgctga tcgccgggca caccgccgga ctgatcgacc ccgccggggt cgaggtggtg cacgtcagct cggcggagca gctgggcaac gcggtgtcca agcacgcggc cgaggccgac gtgctggtga tggccgccgc cgtcgccgac ttccggcccg cccgggttgc cgccgccaag atcaaaaagg gtgtcgaggc accgacgatc gagttggtcc gcaacgacga cgtgctggcc ggcgcggtgc gggcgcgcgc ccacggcgag ctgcccaacc tgcgggccat cgtgggattc gccgccgaga ccggagacgc caacggggac gtgctgtttc acgcccgcgc caagctgcgc aacaagggct gtgacctgtt ggtggtcaac gcggtgggcg agggccgggc cttcgaggtg gacagcaacg acggctggct gctggcggcc gacgggaccg agtcggcgct gcagcacggc tccaagacgc tgatggccag tcgtatcgtg gatgcgatcg ccgccttcct gcgcggggac ggcggtaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDRNRAS TRAARIVVGV SGGIAAYKAC TVVRQLSEAG HSVRVIPTES ALRFVGAATF EALSGQPVHT GVFDDVPEVP HVQLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPVLFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPAAG RLTGTDSGSG RLPEAEEITT LAQLLLERHD ALPYDLAGCK MVVTAGGTRE PVDPVRFIGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTAG LIDPAGVEVV HVSSAEQLGN AVSKHAAEAD VLVMAAAVAD FRPARVAAAK IKKGVEAPTI ELVRNDDVLA GAVRARAHGE LPNLRAIVGF AAETGDANGD VLFHARAKLR NKGCDLLVVN AVGEGRAFEV DSNDGWLLAA DGTESALQHG SKTLMASRIV DAIAAFLRGD GG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTACGAC CGCAATCGGG CTTCCACACG GGCCGCGAGG ATCGTCGTCG GCGTCTCCGG CGGCATCGCC GCCTACAAGG CGTGCACCGT CGTCCGCCAG CTGTCTGAGG CCGGTCATTC GGTCCGGGTC ATCCCCACCG AGTCCGCGCT GCGCTTCGTC GGCGCCGCCA CCTTCGAGGC GCTGTCCGGC CAGCCGGTGC ACACCGGCGT CTTCGACGAC GTGCCCGAGG TGCCGCACGT GCAGCTGGGC AAGCAGGCCG ACCTGGTGGT GGTGGCACCG GCCACCGCCG ACCTGCTGGC CCGTGCGGTG CACGGCCGGG CCGACGACCT GCTGACCGCC ACCCTGCTCA CCGCCCGATG TCCGGTGCTG TTCGCGCCGG CGATGCACAC CGAGATGTGG TTGCACCCGG CCACCGTCGA CAACGTGGCC ACGCTGCGCC GCCGCGGGGC GGTGGTGCTC GAGCCCGCCG CCGGGCGGCT CACCGGCACC GACAGCGGGT CGGGCCGGCT GCCCGAGGCC GAGGAGATCA CCACCCTGGC CCAGCTGCTG CTGGAACGCC ACGACGCGCT GCCCTACGAC CTGGCCGGCT GCAAGATGGT GGTCACCGCC GGCGGCACCC GCGAACCCGT CGACCCGGTG CGCTTCATCG GCAACCGCAG CTCCGGCAAG CAGGGCTACG CGGTGGCCCG GGTGGCCGCC CAGCGCGGCG CCGAGGTCAC GCTGATCGCC GGGCACACCG CCGGACTGAT CGACCCCGCC GGGGTCGAGG TGGTGCACGT CAGCTCGGCG GAGCAGCTGG GCAACGCGGT GTCCAAGCAC GCGGCCGAGG CCGACGTGCT GGTGATGGCC GCCGCCGTCG CCGACTTCCG GCCCGCCCGG GTTGCCGCCG CCAAGATCAA AAAGGGTGTC GAGGCACCGA CGATCGAGTT GGTCCGCAAC GACGACGTGC TGGCCGGCGC GGTGCGGGCG CGCGCCCACG GCGAGCTGCC CAACCTGCGG GCCATCGTGG GATTCGCCGC CGAGACCGGA GACGCCAACG GGGACGTGCT GTTTCACGCC CGCGCCAAGC TGCGCAACAA GGGCTGTGAC CTGTTGGTGG TCAACGCGGT GGGCGAGGGC CGGGCCTTCG AGGTGGACAG CAACGACGGC TGGCTGCTGG CGGCCGACGG GACCGAGTCG GCGCTGCAGC ACGGCTCCAA GACGCTGATG GCCAGTCGTA TCGTGGATGC GATCGCCGCC TTCCTGCGCG GGGACGGCGG AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA