SSGCID: Target Page

MyavA.01254.a

Phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase

CENTER ID:	MyavA.01254.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01254.a.B1.GE36886	Full length( MyavA.01254.a )		1	424
MyavA.01254.a.B3.GE36888	Phosphopantothenate-cysteine ligase		193	424

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3312
RefSeq:	YP_882565.1
UniProt:	A0A0H2ZV65

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYDRNRAFTR AARIVVGVSG GIAAYKACTV VRQLSEAGHS VRVIPTESAL RFVGAATFEA LSGQPVHTGV FNDVPEVPHV QLGKQADLVV VAPATADLLA RAVHGRADDL LTATLLTARC PVLFAPAMHT EMWLHPATVD NVATLRRRGA VVLEPAAGRL TGTDSGSGRL PEAEEITTLA HLLLERHDAL PYDLAGCKML VTAGGTREPV DPVRFIGNRS SGKQGYAVAR VAAQRGAEVT LIAGHTAGLI DPAGVEVVHV SSAAQLGDAV SKRAPEADVL VMAAAVADFR PAHVAAAKIK KSASNETAPP TIELVRNDDV LAGAVRARAH GQLPNMRAIV GFAAETGDAS GDVLFHARAK LQRKGCDLLV VNAVGEGRAF EVDSNDGWLL ASDGTESALQ HGSKTLMASR IVDAIAAFLH GDSG

NT Sequence

GTGTACGACC GCAATCGGGC TTTTACACGG GCCGCGAGGA TCGTCGTCGG CGTCTCCGGC GGCATCGCCG CCTACAAGGC GTGCACCGTC GTCCGCCAGC TGTCTGAGGC CGGTCATTCG GTCCGGGTCA TCCCGACCGA GTCCGCGCTG CGTTTCGTCG GCGCGGCCAC CTTTGAGGCG CTCTCCGGCC AGCCGGTGCA CACCGGCGTC TTCAACGACG TTCCCGAGGT CCCGCACGTC CAGCTCGGCA AGCAGGCCGA CCTGGTCGTG GTGGCGCCGG CGACGGCGGA TCTGCTGGCG CGCGCGGTGC ACGGCCGGGC CGACGATCTG CTGACCGCCA CGCTGCTCAC CGCCCGATGC CCGGTGCTGT TCGCGCCGGC CATGCACACC GAGATGTGGT TGCACCCGGC CACCGTCGAC AACGTGGCCA CGTTGCGCCG GCGGGGCGCG GTGGTGCTCG AGCCCGCCGC CGGGCGCCTC ACCGGGACGG ACAGCGGGTC GGGCCGGCTG CCCGAAGCCG AGGAGATCAC CACCCTGGCC CACCTGCTGC TGGAACGCCA CGACGCGCTG CCCTACGACC TCGCCGGCTG CAAGATGCTG GTCACCGCCG GCGGCACCCG CGAACCGGTC GACCCGGTGC GCTTCATCGG CAACCGCAGC TCTGGCAAGC AGGGCTATGC CGTGGCGCGG GTGGCGGCCC AGCGCGGCGC CGAGGTCACC CTGATCGCCG GGCACACCGC CGGGCTGATC GATCCCGCCG GTGTCGAGGT CGTCCACGTC AGCTCCGCGG CCCAGCTGGG CGACGCGGTG TCCAAGCGCG CCCCGGAGGC CGACGTGCTG GTGATGGCCG CCGCGGTCGC CGACTTCCGG CCCGCGCACG TTGCCGCCGC GAAGATCAAA AAGAGCGCCT CGAATGAGAC GGCGCCCCCG ACGATCGAGC TGGTGCGCAA CGACGACGTG CTCGCCGGGG CGGTGCGGGC CCGCGCCCAC GGGCAGTTGC CCAACATGCG GGCCATCGTC GGCTTTGCCG CCGAGACCGG CGACGCCAGC GGCGACGTGC TGTTTCACGC CCGGGCCAAG CTGCAGCGCA AGGGCTGCGA TCTGCTGGTG GTCAACGCCG TCGGCGAGGG CCGCGCGTTC GAGGTGGACA GCAACGACGG CTGGCTGCTG GCCTCCGACG GCACCGAGTC CGCACTGCAG CACGGCTCCA AGACATTGAT GGCCAGTCGT ATCGTGGATG CGATCGCCGC GTTCCTGCAC GGGGACAGCG GG

Details for MyavA.01254.a.B1.GE36886

HARVESTED ON:	5/1/2013
SEQUENCED ON:	3/3/2015
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMV YDRNRAFTRA ARIVVGVSGG IAAYKACTVV RQLSEAGHSV RVIPTESALR FVGAATFEAL SGQPVHTGVF NDVPEVPHVQ LGKQADLVVV APATADLLAR AVHGRADDLL TATLLTARCP VLFAPAMHTE MWLHPATVDN VATLRRRGAV VLEPAAGRLT GTDSGSGRLP EAEEITTLAH LLLERHDALP YDLAGCKMLV TAGGTREPVD PVRFIGNRSS GKQGYAVARV AAQRGAEVTL IAGHTAGLID PAGVEVVHVS SAAQLGDAVS KRAPEADVLV MAAAVADFRP AHVAAAKIKK SASNETAPPT IELVRNDDVL AGAVRARAHG QLPNMRAIVG FAAETGDASG DVLFHARAKL QRKGCDLLVV NAVGEGRAFE VDSNDGWLLA SDGTESALQH GSKTLMASRI VDAIA

Validated NT Sequence

atggctcacc accaccacca ccatatggtg tacgaccgca atcgggcttt tacacgggcc gcgaggatcg tcgtcggcgt ctccggcggc atcgccgcct acaaggcgtg caccgtcgtc cgccagctgt ctgaggccgg tcattcggtc cgggtcatcc cgaccgagtc cgcgctgcgt ttcgtcggcg cggccacctt tgaggcgctc tccggccagc cggtgcacac cggcgtcttc aacgacgttc ccgaggtccc gcacgtccag ctcggcaagc aggccgacct ggtcgtggtg gcgccggcga cggcggatct gctggcgcgc gcggtgcacg gccgggccga cgatctgctg accgccacgc tgctcaccgc ccgatgcccg gtgctgttcg cgccggccat gcacaccgag atgtggttgc acccggccac cgtcgacaac gtggccacgt tgcgccggcg gggcgcggtg gtgctcgagc ccgccgccgg gcgcctcacc gggacggaca gcgggtcggg ccggctgccc gaagccgagg agatcaccac cctggcccac ctgctgctgg aacgccacga cgcgctgccc tacgacctcg ccggctgcaa gatgctggtc accgccggcg gcacccgcga accggtcgac ccggtgcgct tcatcggcaa ccgcagctct ggcaagcagg gctatgccgt ggcgcgggtg gcggcccagc gcggcgccga ggtcaccctg atcgccgggc acaccgccgg gctgatcgat cccgccggtg tcgaggtcgt ccacgtcagc tccgcggccc agctgggcga cgcggtgtcc aagcgcgccc cggaggccga cgtgctggtg atggccgccg cggtcgccga cttccggccc gcgcacgttg ccgccgcgaa gatcaaaaag agcgcctcga atgagacggc gcccccgacg atcgagctgg tgcgcaacga cgacgtgctc gccggggcgg tgcgggcccg cgcccacggg cagttgccca acatgcgggc catcgtcggc tttgccgccg agaccggcga cgccagcggc gacgtgctgt ttcacgcccg ggccaagctg cagcgcaagg gctgcgatct gctggtggtc aacgccgtcg gcgagggccg cgcgttcgag gtggacagca acgacggctg gctgctggcc tccgacggca ccgagtccgc actgcagcac ggctccaaga cattgatggc cagtcgtatc gtggatgcga tcgccg

Expected Protein Sequence

MAHHHHHHMY DRNRAFTRAA RIVVGVSGGI AAYKACTVVR QLSEAGHSVR VIPTESALRF VGAATFEALS GQPVHTGVFN DVPEVPHVQL GKQADLVVVA PATADLLARA VHGRADDLLT ATLLTARCPV LFAPAMHTEM WLHPATVDNV ATLRRRGAVV LEPAAGRLTG TDSGSGRLPE AEEITTLAHL LLERHDALPY DLAGCKMLVT AGGTREPVDP VRFIGNRSSG KQGYAVARVA AQRGAEVTLI AGHTAGLIDP AGVEVVHVSS AAQLGDAVSK RAPEADVLVM AAAVADFRPA HVAAAKIKKS ASNETAPPTI ELVRNDDVLA GAVRARAHGQ LPNMRAIVGF AAETGDASGD VLFHARAKLQ RKGCDLLVVN AVGEGRAFEV DSNDGWLLAS DGTESALQHG SKTLMASRIV DAIAAFLHGD SG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtacg accgcaatcg ggcttttaca cgggccgcga ggatcgtcgt cggcgtctcc ggcggcatcg ccgcctacaa ggcgtgcacc gtcgtccgcc agctgtctga ggccggtcat tcggtccggg tcatcccgac cgagtccgcg ctgcgtttcg tcggcgcggc cacctttgag gcgctctccg gccagccggt gcacaccggc gtcttcaacg acgttcccga ggtcccgcac gtccagctcg gcaagcaggc cgacctggtc gtggtggcgc cggcgacggc ggatctgctg gcgcgcgcgg tgcacggccg ggccgacgat ctgctgaccg ccacgctgct caccgcccga tgcccggtgc tgttcgcgcc ggccatgcac accgagatgt ggttgcaccc ggccaccgtc gacaacgtgg ccacgttgcg ccggcggggc gcggtggtgc tcgagcccgc cgccgggcgc ctcaccggga cggacagcgg gtcgggccgg ctgcccgaag ccgaggagat caccaccctg gcccacctgc tgctggaacg ccacgacgcg ctgccctacg acctcgccgg ctgcaagatg ctggtcaccg ccggcggcac ccgcgaaccg gtcgacccgg tgcgcttcat cggcaaccgc agctctggca agcagggcta tgccgtggcg cgggtggcgg cccagcgcgg cgccgaggtc accctgatcg ccgggcacac cgccgggctg atcgatcccg ccggtgtcga ggtcgtccac gtcagctccg cggcccagct gggcgacgcg gtgtccaagc gcgccccgga ggccgacgtg ctggtgatgg ccgccgcggt cgccgacttc cggcccgcgc acgttgccgc cgcgaagatc aaaaagagcg cctcgaatga gacggcgccc ccgacgatcg agctggtgcg caacgacgac gtgctcgccg gggcggtgcg ggcccgcgcc cacgggcagt tgcccaacat gcgggccatc gtcggctttg ccgccgagac cggcgacgcc agcggcgacg tgctgtttca cgcccgggcc aagctgcagc gcaagggctg cgatctgctg gtggtcaacg ccgtcggcga gggccgcgcg ttcgaggtgg acagcaacga cggctggctg ctggcctccg acggcaccga gtccgcactg cagcacggct ccaagacatt gatggccagt cgtatcgtgg atgcgatcgc cgcgttcctg cacggggaca gcgggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyavA.01254.a.B3.GE36888

HARVESTED ON:	5/1/2013
SEQUENCED ON:	3/3/2015
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD LAGCKMLVTA GGTREPVDPV RFIGNRSSGK QGYAVARVAA QRGAEVTLIA GHTAGLIDPA GVEVVHVSSA AQLGDAVSKR APEADVLVMA AAVADFRPAH VAAAKIKKSA SNETAPPTIE LVRNDDVLAG AVRARAHGQL PNMRAIVGFA AETGDASGDV LFHARAKLQR KGCDLLVVNA VGEGRAFEVD SNDGWLLASD GTESALQHGS KTLMASRIVD AIAAFLHGDS G

Validated NT Sequence

atggctcacc accaccacca ccatatggac ctcgccggct gcaagatgct ggtcaccgcc ggcggcaccc gcgaaccggt cgacccggtg cgcttcatcg gcaaccgcag ctctggcaag cagggctatg ccgtggcgcg ggtggcggcc cagcgcggcg ccgaggtcac cctgatcgcc gggcacaccg ccgggctgat cgatcccgcc ggtgtcgagg tcgtccacgt cagctccgcg gcccagctgg gcgacgcggt gtccaagcgc gccccggagg ccgacgtgct ggtgatggcc gccgcggtcg ccgacttccg gcccgcgcac gttgccgccg cgaagatcaa aaagagcgcc tcgaatgaga cggcgccccc gacgatcgag ctggtgcgca acgacgacgt gctcgccggg gcggtgcggg cccgcgccca cgggcagttg cccaacatgc gggccatcgt cggctttgcc gccgagaccg gcgacgccag cggcgacgtg ctgtttcacg cccgggccaa gctgcagcgc aagggctgcg atctgctggt ggtcaacgcc gtcggcgagg gccgcgcgtt cgaggtggac agcaacgacg gctggctgct ggcctccgac ggcaccgagt ccgcactgca gcacggctcc aagacattga tggccagtcg tatcgtggat gcgatcgccg cgttcctgca cggggacagc gggtaagtga gtaagat

Expected Protein Sequence

MAHHHHHHDL AGCKMLVTAG GTREPVDPVR FIGNRSSGKQ GYAVARVAAQ RGAEVTLIAG HTAGLIDPAG VEVVHVSSAA QLGDAVSKRA PEADVLVMAA AVADFRPAHV AAAKIKKSAS NETAPPTIEL VRNDDVLAGA VRARAHGQLP NMRAIVGFAA ETGDASGDVL FHARAKLQRK GCDLLVVNAV GEGRAFEVDS NDGWLLASDG TESALQHGSK TLMASRIVDA IAAFLHGDSG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacctcg ccggctgcaa gatgctggtc accgccggcg gcacccgcga accggtcgac ccggtgcgct tcatcggcaa ccgcagctct ggcaagcagg gctatgccgt ggcgcgggtg gcggcccagc gcggcgccga ggtcaccctg atcgccgggc acaccgccgg gctgatcgat cccgccggtg tcgaggtcgt ccacgtcagc tccgcggccc agctgggcga cgcggtgtcc aagcgcgccc cggaggccga cgtgctggtg atggccgccg cggtcgccga cttccggccc gcgcacgttg ccgccgcgaa gatcaaaaag agcgcctcga atgagacggc gcccccgacg atcgagctgg tgcgcaacga cgacgtgctc gccggggcgg tgcgggcccg cgcccacggg cagttgccca acatgcgggc catcgtcggc tttgccgccg agaccggcga cgccagcggc gacgtgctgt ttcacgcccg ggccaagctg cagcgcaagg gctgcgatct gctggtggtc aacgccgtcg gcgagggccg cgcgttcgag gtggacagca acgacggctg gctgctggcc tccgacggca ccgagtccgc actgcagcac ggctccaaga cattgatggc cagtcgtatc gtggatgcga tcgccgcgtt cctgcacggg gacagcgggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat