MyabA.01254.a

Putative pantothenate metabolism flavoprotein

CENTER ID: MyabA.01254.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01254.a.A1.GE30492 Full length( MyabA.01254.a ) 1 415
MyabA.01254.a.AE1.GE43336 Full length( MyabA.01254.a ) 1 415
MyabA.01254.a.B2.GE36884 Phosphopantothenoylcysteine decarboxylase 2 186
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01254.a.B2.PW37341 Phosphopantothenoylcysteine decarboxylase 2 186
MyabA.01254.a.B2.PW37342 Phosphopantothenoylcysteine decarboxylase 2 186
MyabA.01254.a.A1.PW37442 Full length( MyabA.01254.a ) 1 415 unavailable
MyabA.01254.a.A1.PW37463 Full length( MyabA.01254.a ) 1 415
MyabA.01254.a.A1.PW37464 Full length( MyabA.01254.a ) 1 415

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2868
RefSeq: YP_001703554.1
UniProt: B1MCC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRIIVGVGG GIAAYKACTV VRQLAEAGHS VRVVPTESAL QFVGAATFEA LSGEPARTGV FDDVPEVPHV RLGQDADLVV VAPATADLLS RAATGRADDL LTATLLTATC PVLFAPAMHT EMWLHPATQQ NVATLRSRGA VVLEPASGRL TGADTGPGRL PEAEEITTLA QLLLARPDAL PHDLAGVRVL VTAGGTREPL DPVRFIGNRS SGKQGYAIAR VAAQRGAEVT LIAGHTVGLP DPAGVEVVHI SSAAQLQDAV TKHAPAAEAL IMAAAVADFR PAHVATSKIK KSHGAAGEND APIELLRNAD VLAGVVRARA DGQLPKMRAI VGFAAETGDA NGDVLFHARA KLKRKGCDLL VVNAVGEGRA FEVDSNDGWL LSAQSGGDIT EVVLEHGTKV LMASRIVDAV AGLCQ
NT Sequence
ATGTCCCGGA TCATCGTCGG AGTGGGTGGA GGAATCGCCG CCTACAAGGC CTGCACCGTG GTGCGCCAGC TCGCCGAGGC CGGGCACAGC GTCCGGGTCG TTCCCACCGA GTCGGCCCTG CAGTTCGTGG GAGCCGCGAC CTTTGAGGCG CTCTCCGGTG AGCCGGCCCG TACCGGGGTG TTCGACGATG TTCCCGAGGT TCCGCACGTG CGGCTCGGAC AGGATGCCGA CCTGGTAGTG GTCGCTCCCG CCACCGCGGA CCTGCTGTCG CGTGCCGCCA CCGGCCGGGC GGATGACCTG CTGACCGCGA CCCTGCTCAC CGCCACGTGT CCAGTGCTGT TCGCCCCCGC GATGCATACG GAGATGTGGC TGCATCCGGC AACCCAGCAG AATGTCGCGA CCCTGCGGAG CCGTGGCGCG GTTGTCCTGG AGCCCGCCTC TGGCCGCCTG ACGGGGGCGG ACACCGGCCC GGGCCGTCTT CCCGAGGCCG AAGAAATCAC CACCCTGGCC CAATTATTGC TGGCACGACC CGACGCGCTG CCGCACGATC TGGCCGGTGT GCGGGTCCTG GTCACCGCCG GCGGCACTCG TGAACCCCTG GACCCGGTGC GCTTTATCGG AAACCGCAGT TCCGGTAAGC AGGGATACGC CATCGCCCGG GTCGCCGCTC AGCGTGGTGC CGAAGTCACG CTCATCGCGG GTCATACCGT CGGCTTACCG GATCCCGCTG GCGTCGAGGT GGTGCATATC AGCTCCGCCG CGCAATTGCA GGACGCTGTC ACCAAACACG CTCCTGCGGC CGAAGCGCTG ATCATGGCGG CAGCGGTGGC AGATTTCCGG CCGGCCCACG TTGCGACCAG CAAGATCAAA AAGAGCCACG GGGCCGCCGG TGAGAATGAC GCGCCCATTG AGTTGCTGCG CAACGCCGAT GTGCTGGCCG GTGTGGTTCG TGCGCGTGCA GACGGGCAGC TGCCCAAGAT GCGGGCAATC GTCGGGTTTG CCGCGGAAAC GGGCGACGCA AACGGCGATG TGCTCTTTCA CGCCCGCGCC AAGTTGAAGC GCAAGGGCTG TGATTTGTTG GTAGTCAACG CTGTTGGCGA GGGACGGGCC TTCGAGGTGG ACAGCAATGA CGGGTGGTTG CTCTCGGCGC AGAGTGGTGG TGACATCACC GAAGTTGTCC TTGAACACGG CACCAAAGTT CTGATGGCAA GCCGTATTGT GGATGCCGTT GCCGGGCTTT GTCAG
Details for MyabA.01254.a.A1.GE30492
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMSRIIVG VGGGIXAYXA CTVVRQLAEA GHSVRVVPXE SALQFVGAAT FEALSGEPAR TGVFDDVPXV PHVRLGQDAX LVXVAPAXAD LLSRAATGRA DDLLTATLLT ATCPVLFAPA MHTEMWLHPA TQQNVATLRS RGAVVLEPAS GRLTGADTGP GRLPEAEEIT TLAQLLLARP DALPHDLAGV RVLVTAGGTR EPLDPVRFIG NRSSGKQGYA IARVAAQRGA EVTLIAGHTV GLPDPAGVEV VHISSAAQLQ DAVTKHAPAA EALIMAAAVA DFRPAHVATS KIKKSHGAAG ENDAPIELLR NADVLAGVVR ARADGQLPKM RAIVGFAAET GDANGDVLFH ARAKLKRKGC DLLVVNAVGE GRAFEVDSND GWLLSAQSGG DITEVVLEHG TKVLMASRIV DAVAGLCQ
Validated NT Sequence
ttattactga caaagcccgg caacggcatc cacaatacgg cttgccatca gaactttggt gccgtgttca aggacaactt cggtgatgtc accaccactc tgcgccgaga gcaaccaccc gtcattgctg tccacctcga aggcccgtcc ctcgccaaca gcgttgacta ccaacaaatc acagcccttg cgcttcaact tggcgcgggc gtgaaagagc acatcgccgt ttgcgtcgcc cgtttccgcg gcaaacccga cgattgcccg catcttgggc agctgcccgt ctgcacgcgc acgaaccaca ccggccagca catcggcgtt gcgcagcaac tcaatgggcg cgtcattctc accggcggcc ccgtggctct ttttgatctt gctggtcgca acgtgggccg gccggaaatc tgccaccgct gccgccatga tcagcgcttc ggccgcagga gcgtgtttgg tgacagcgtc ctgcaattgc gcggcggagc tgatatgcac cacctcgacg ccagcgggat ccggtaagcc gacggtatga cccgcgatga gcgtgacttc ggcaccacgc tgagcggcga cccgggcgat ggcgtatccc tgcttaccgg aactgcggtt tccgataaag cgcaccgggt ccaggggttc acgagtgccg ccggcggtga ccaggacccg cacaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggng gcgggagcga ccantaccag gtnggcatcc tgtccgagcc gcacgtgcgg aacctnggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggnggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggcctngtag gcggngattc ctccacccac tccgacgatg atccgggaca tcgaaccagg accctgggtc tgagcttcca gggtacccat a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIIVGVG GGIAAYKACT VVRQLAEAGH SVRVVPTESA LQFVGAATFE ALSGEPARTG VFDDVPEVPH VRLGQDADLV VVAPATADLL SRAATGRADD LLTATLLTAT CPVLFAPAMH TEMWLHPATQ QNVATLRSRG AVVLEPASGR LTGADTGPGR LPEAEEITTL AQLLLARPDA LPHDLAGVRV LVTAGGTREP LDPVRFIGNR SSGKQGYAIA RVAAQRGAEV TLIAGHTVGL PDPAGVEVVH ISSAAQLQDA VTKHAPAAEA LIMAAAVADF RPAHVATSKI KKSHGAAGEN DAPIELLRNA DVLAGVVRAR ADGQLPKMRA IVGFAAETGD ANGDVLFHAR AKLKRKGCDL LVVNAVGEGR AFEVDSNDGW LLSAQSGGDI TEVVLEHGTK VLMASRIVDA VAGLCQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcccggatc atcgtcggag tgggtggagg aatcgccgcc tacaaggcct gcaccgtggt gcgccagctc gccgaggccg ggcacagcgt ccgggtcgtt cccaccgagt cggccctgca gttcgtggga gccgcgacct ttgaggcgct ctccggtgag ccggcccgta ccggggtgtt cgacgatgtt cccgaggttc cgcacgtgcg gctcggacag gatgccgacc tggtagtggt cgctcccgcc accgcggacc tgctgtcgcg tgccgccacc ggccgggcgg atgacctgct gaccgcgacc ctgctcaccg ccacgtgtcc agtgctgttc gcccccgcga tgcatacgga gatgtggctg catccggcaa cccagcagaa tgtcgcgacc ctgcggagcc gtggcgcggt tgtcctggag cccgcctctg gccgcctgac gggggcggac accggcccgg gccgtcttcc cgaggccgaa gaaatcacca ccctggccca attattgctg gcacgacccg acgcgctgcc gcacgatctg gccggtgtgc gggtcctggt caccgccggc ggcactcgtg aacccctgga cccggtgcgc tttatcggaa accgcagttc cggtaagcag ggatacgcca tcgcccgggt cgccgctcag cgtggtgccg aagtcacgct catcgcgggt cataccgtcg gcttaccgga tcccgctggc gtcgaggtgg tgcatatcag ctccgccgcg caattgcagg acgctgtcac caaacacgct cctgcggccg aagcgctgat catggcggca gcggtggcag atttccggcc ggcccacgtt gcgaccagca agatcaaaaa gagccacggg gccgccggtg agaatgacgc gcccattgag ttgctgcgca acgccgatgt gctggccggt gtggttcgtg cgcgtgcaga cgggcagctg cccaagatgc gggcaatcgt cgggtttgcc gcggaaacgg gcgacgcaaa cggcgatgtg ctctttcacg cccgcgccaa gttgaagcgc aagggctgtg atttgttggt agtcaacgct gttggcgagg gacgggcctt cgaggtggac agcaatgacg ggtggttgct ctcggcgcag agtggtggtg acatcaccga agttgtcctt gaacacggca ccaaagttct gatggcaagc cgtattgtgg atgccgttgc cgggctttgt cagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyabA.01254.a.AE1.GE43336
HARVESTED ON: data unavailable
SEQUENCED ON: 8/7/2019
EXPECTED MW: 44kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MSRIIVGVGG GIAAYKACTV VRQLAEAGHS VRVVPTESAL QFVGAATFEA LSGEPARTGV FDDVPEVPHV RLGQDADLVV VAPATADLLS RAATGRADDL LTATLLTATC PVLFAPAMHT EMWLHPATQQ NVATLRSRGA VVLEPASGRL TGADTGPGRL PEAEEITTLA QLLLARPDAL PHDLAGVRVL VTAGGTREPL DPVRFIGNRS SGKQGYAIAR VAAQRGAEVT LIAGHTVGLP DPAGVEVVHI SSAAQLQDAV TKHAPAAEAL IMAAAVADFR PAHVATSKIK KSHGAAGEND APIELLRNAD VLAGVVRARA DGQLPKMRAI VGFAAETGDA NGDVLFHARA KLKRKGCDLL VVNAVGEGRA FEVDSNDGWL LSAQSGGDIT EVVLEHGTKV LMASRIVDAV AGLCQAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cctgacaaag cccggcaacg gcatccacaa tacggcttgc catcagaact ttggtgccgt gttcaaggac aacttcggtg atgtcaccac cactctgcgc cgagagcaac cacccgtcat tgctgtccac ctcgaaggcc cgtccctcgc caacagcgtt gactaccaac aaatcacagc ccttgcgctt caacttggcg cgggcgtgaa agagcacatc gccgtttgcg tcgcccgttt ccgcggcaaa cccgacgatt gcccgcatct tgggcagctg cccgtctgca cgcgcacgaa ccacaccggc cagcacatcg gcgttgcgca gcaactcaat gggcgcgtca ttctcaccgg cggccccgtg gctctttttg atcttgctgg tcgcaacgtg ggccggccgg aaatctgcca ccgctgccgc catgatcagc gcttcggccg caggagcgtg tttggtgaca gcgtcctgca attgcgcggc ggagctgata tgcaccacct cgacgccagc gggatccggt aagccgacgg tatgacccgc gatgagcgtg acttcggcac cacgctgagc ggcgacccgg gcgatggcgt atccctgctt accggaactg cggtttccga taaagcgcac cgggtccagg ggttcacgag tgccgccggc ggtgaccagg acccgcacac cggccagatc gtgcggcagc gcgtcgggtc gtgccagcaa taattgggcc agggtggtga tttcttcggc ctcgggaaga cggcccgggc cggtgtccgc ccccgtcagg cggccagagg cgggctccag gacaaccgcg ccacggctcc gcagggtcgc gacattctgc tgggttgccg gatgcagcca catctccgta tgcatcgcgg gggcgaacag cactggacac gtggcggtga gcagggtcgc ggtcagcagg tcatccgccc ggccggtggc ggcacgcgac agcaggtccg cggtggcggg agcgaccact accaggtcgg catcctgtcc gagccgcacg tgcggaacct cgggaacatc gtcgaacacc ccggtacggg ccggctcacc ggagagcgcc tcaaaggtcg cggctcccac gaactgcagg gccgactcgg tgggaacgac ccggacgctg tgcccggcct cggcgagctg gcgcaccacg gtgcaggcct tgtaggcggc gattcctcca cccactccga cgatgatccg ggacatggga gaga
Expected Protein Sequence
MSRIIVGVGG GIAAYKACTV VRQLAEAGHS VRVVPTESAL QFVGAATFEA LSGEPARTGV FDDVPEVPHV RLGQDADLVV VAPATADLLS RAATGRADDL LTATLLTATC PVLFAPAMHT EMWLHPATQQ NVATLRSRGA VVLEPASGRL TGADTGPGRL PEAEEITTLA QLLLARPDAL PHDLAGVRVL VTAGGTREPL DPVRFIGNRS SGKQGYAIAR VAAQRGAEVT LIAGHTVGLP DPAGVEVVHI SSAAQLQDAV TKHAPAAEAL IMAAAVADFR PAHVATSKIK KSHGAAGEND APIELLRNAD VLAGVVRARA DGQLPKMRAI VGFAAETGDA NGDVLFHARA KLKRKGCDLL VVNAVGEGRA FEVDSNDGWL LSAQSGGDIT EVVLEHGTKV LMASRIVDAV AGLCQGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtcccggatc atcgtcggag tgggtggagg aatcgccgcc tacaaggcct gcaccgtggt gcgccagctc gccgaggccg ggcacagcgt ccgggtcgtt cccaccgagt cggccctgca gttcgtggga gccgcgacct ttgaggcgct ctccggtgag ccggcccgta ccggggtgtt cgacgatgtt cccgaggttc cgcacgtgcg gctcggacag gatgccgacc tggtagtggt cgctcccgcc accgcggacc tgctgtcgcg tgccgccacc ggccgggcgg atgacctgct gaccgcgacc ctgctcaccg ccacgtgtcc agtgctgttc gcccccgcga tgcatacgga gatgtggctg catccggcaa cccagcagaa tgtcgcgacc ctgcggagcc gtggcgcggt tgtcctggag cccgcctctg gccgcctgac gggggcggac accggcccgg gccgtcttcc cgaggccgaa gaaatcacca ccctggccca attattgctg gcacgacccg acgcgctgcc gcacgatctg gccggtgtgc gggtcctggt caccgccggc ggcactcgtg aacccctgga cccggtgcgc tttatcggaa accgcagttc cggtaagcag ggatacgcca tcgcccgggt cgccgctcag cgtggtgccg aagtcacgct catcgcgggt cataccgtcg gcttaccgga tcccgctggc gtcgaggtgg tgcatatcag ctccgccgcg caattgcagg acgctgtcac caaacacgct cctgcggccg aagcgctgat catggcggca gcggtggcag atttccggcc ggcccacgtt gcgaccagca agatcaaaaa gagccacggg gccgccggtg agaatgacgc gcccattgag ttgctgcgca acgccgatgt gctggccggt gtggttcgtg cgcgtgcaga cgggcagctg cccaagatgc gggcaatcgt cgggtttgcc gcggaaacgg gcgacgcaaa cggcgatgtg ctctttcacg cccgcgccaa gttgaagcgc aagggctgtg atttgttggt agtcaacgct gttggcgagg gacgggcctt cgaggtggac agcaatgacg ggtggttgct ctcggcgcag agtggtggtg acatcaccga agttgtcctt gaacacggca ccaaagttct gatggcaagc cgtattgtgg atgccgttgc cgggctttgt caggggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for MyabA.01254.a.B2.GE36884
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS RIIVGVGGGI AAYKACTVVR QLAEAGHSVR VVPTESALQF VGAATFEALS GEPARTGVFD DVPEVPHVRL GQDADLVVVA PATADLLSRA ATGRADDLLT ATLLTATCPV LFAPAMHTEM WLHPATQQNV ATLRSRGAVV LEPASGRLTG ADTGPGRLPE AEEITTLAQL LLARPDALPH DLAG
Validated NT Sequence
ttgttagcag ccggatccta tcttactcac ttaaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggtg gcgggagcga ccactaccag gtcggcatcc tgtccgagcc gcacgtgcgg aacctcggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggtggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggccttgtag gcggcgattc ctccacccac tccgacgatg atccgggaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHSR IIVGVGGGIA AYKACTVVRQ LAEAGHSVRV VPTESALQFV GAATFEALSG EPARTGVFDD VPEVPHVRLG QDADLVVVAP ATADLLSRAA TGRADDLLTA TLLTATCPVL FAPAMHTEMW LHPATQQNVA TLRSRGAVVL EPASGRLTGA DTGPGRLPEA EEITTLAQLL LARPDALPHD LAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcccgga tcatcgtcgg agtgggtgga ggaatcgccg cctacaaggc ctgcaccgtg gtgcgccagc tcgccgaggc cgggcacagc gtccgggtcg ttcccaccga gtcggccctg cagttcgtgg gagccgcgac ctttgaggcg ctctccggtg agccggcccg taccggggtg ttcgacgatg ttcccgaggt tccgcacgtg cggctcggac aggatgccga cctggtagtg gtcgctcccg ccaccgcgga cctgctgtcg cgtgccgcca ccggccgggc ggatgacctg ctgaccgcga ccctgctcac cgccacgtgt ccagtgctgt tcgcccccgc gatgcatacg gagatgtggc tgcatccggc aacccagcag aatgtcgcga ccctgcggag ccgtggcgcg gttgtcctgg agcccgcctc tggccgcctg acgggggcgg acaccggccc gggccgtctt cccgaggccg aagaaatcac caccctggcc caattattgc tggcacgacc cgacgcgctg ccgcacgatc tggccggttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MyabA.01254.a.B2.PW37341
PURIFICATION DATe: 8/5/2013
CONCENTRATION: 22.4mg/ml
OBSERVED MW: 20kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS RIIVGVGGGI AAYKACTVVR QLAEAGHSVR VVPTESALQF VGAATFEALS GEPARTGVFD DVPEVPHVRL GQDADLVVVA PATADLLSRA ATGRADDLLT ATLLTATCPV LFAPAMHTEM WLHPATQQNV ATLRSRGAVV LEPASGRLTG ADTGPGRLPE AEEITTLAQL LLARPDALPH DLAG
Validated NT Sequence
ttgttagcag ccggatccta tcttactcac ttaaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggtg gcgggagcga ccactaccag gtcggcatcc tgtccgagcc gcacgtgcgg aacctcggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggtggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggccttgtag gcggcgattc ctccacccac tccgacgatg atccgggaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHSR IIVGVGGGIA AYKACTVVRQ LAEAGHSVRV VPTESALQFV GAATFEALSG EPARTGVFDD VPEVPHVRLG QDADLVVVAP ATADLLSRAA TGRADDLLTA TLLTATCPVL FAPAMHTEMW LHPATQQNVA TLRSRGAVVL EPASGRLTGA DTGPGRLPEA EEITTLAQLL LARPDALPHD LAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCCGGA TCATCGTCGG AGTGGGTGGA GGAATCGCCG CCTACAAGGC CTGCACCGTG GTGCGCCAGC TCGCCGAGGC CGGGCACAGC GTCCGGGTCG TTCCCACCGA GTCGGCCCTG CAGTTCGTGG GAGCCGCGAC CTTTGAGGCG CTCTCCGGTG AGCCGGCCCG TACCGGGGTG TTCGACGATG TTCCCGAGGT TCCGCACGTG CGGCTCGGAC AGGATGCCGA CCTGGTAGTG GTCGCTCCCG CCACCGCGGA CCTGCTGTCG CGTGCCGCCA CCGGCCGGGC GGATGACCTG CTGACCGCGA CCCTGCTCAC CGCCACGTGT CCAGTGCTGT TCGCCCCCGC GATGCATACG GAGATGTGGC TGCATCCGGC AACCCAGCAG AATGTCGCGA CCCTGCGGAG CCGTGGCGCG GTTGTCCTGG AGCCCGCCTC TGGCCGCCTG ACGGGGGCGG ACACCGGCCC GGGCCGTCTT CCCGAGGCCG AAGAAATCAC CACCCTGGCC CAATTATTGC TGGCACGACC CGACGCGCTG CCGCACGATC TGGCCGGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for MyabA.01254.a.B2.PW37342
PURIFICATION DATe: 8/5/2013
CONCENTRATION: 21.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS RIIVGVGGGI AAYKACTVVR QLAEAGHSVR VVPTESALQF VGAATFEALS GEPARTGVFD DVPEVPHVRL GQDADLVVVA PATADLLSRA ATGRADDLLT ATLLTATCPV LFAPAMHTEM WLHPATQQNV ATLRSRGAVV LEPASGRLTG ADTGPGRLPE AEEITTLAQL LLARPDALPH DLAG
Validated NT Sequence
ttgttagcag ccggatccta tcttactcac ttaaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggtg gcgggagcga ccactaccag gtcggcatcc tgtccgagcc gcacgtgcgg aacctcggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggtggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggccttgtag gcggcgattc ctccacccac tccgacgatg atccgggaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHSR IIVGVGGGIA AYKACTVVRQ LAEAGHSVRV VPTESALQFV GAATFEALSG EPARTGVFDD VPEVPHVRLG QDADLVVVAP ATADLLSRAA TGRADDLLTA TLLTATCPVL FAPAMHTEMW LHPATQQNVA TLRSRGAVVL EPASGRLTGA DTGPGRLPEA EEITTLAQLL LARPDALPHD LAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCCGGA TCATCGTCGG AGTGGGTGGA GGAATCGCCG CCTACAAGGC CTGCACCGTG GTGCGCCAGC TCGCCGAGGC CGGGCACAGC GTCCGGGTCG TTCCCACCGA GTCGGCCCTG CAGTTCGTGG GAGCCGCGAC CTTTGAGGCG CTCTCCGGTG AGCCGGCCCG TACCGGGGTG TTCGACGATG TTCCCGAGGT TCCGCACGTG CGGCTCGGAC AGGATGCCGA CCTGGTAGTG GTCGCTCCCG CCACCGCGGA CCTGCTGTCG CGTGCCGCCA CCGGCCGGGC GGATGACCTG CTGACCGCGA CCCTGCTCAC CGCCACGTGT CCAGTGCTGT TCGCCCCCGC GATGCATACG GAGATGTGGC TGCATCCGGC AACCCAGCAG AATGTCGCGA CCCTGCGGAG CCGTGGCGCG GTTGTCCTGG AGCCCGCCTC TGGCCGCCTG ACGGGGGCGG ACACCGGCCC GGGCCGTCTT CCCGAGGCCG AAGAAATCAC CACCCTGGCC CAATTATTGC TGGCACGACC CGACGCGCTG CCGCACGATC TGGCCGGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for MyabA.01254.a.A1.PW37442
PURIFICATION DATe: 2/10/2014
CONCENTRATION: 10.4mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSRIIVG VGGGIXAYXA CTVVRQLAEA GHSVRVVPXE SALQFVGAAT FEALSGEPAR TGVFDDVPXV PHVRLGQDAX LVXVAPAXAD LLSRAATGRA DDLLTATLLT ATCPVLFAPA MHTEMWLHPA TQQNVATLRS RGAVVLEPAS GRLTGADTGP GRLPEAEEIT TLAQLLLARP DALPHDLAGV RVLVTAGGTR EPLDPVRFIG NRSSGKQGYA IARVAAQRGA EVTLIAGHTV GLPDPAGVEV VHISSAAQLQ DAVTKHAPAA EALIMAAAVA DFRPAHVATS KIKKSHGAAG ENDAPIELLR NADVLAGVVR ARADGQLPKM RAIVGFAAET GDANGDVLFH ARAKLKRKGC DLLVVNAVGE GRAFEVDSND GWLLSAQSGG DITEVVLEHG TKVLMASRIV DAVAGLCQ
Validated NT Sequence
ttattactga caaagcccgg caacggcatc cacaatacgg cttgccatca gaactttggt gccgtgttca aggacaactt cggtgatgtc accaccactc tgcgccgaga gcaaccaccc gtcattgctg tccacctcga aggcccgtcc ctcgccaaca gcgttgacta ccaacaaatc acagcccttg cgcttcaact tggcgcgggc gtgaaagagc acatcgccgt ttgcgtcgcc cgtttccgcg gcaaacccga cgattgcccg catcttgggc agctgcccgt ctgcacgcgc acgaaccaca ccggccagca catcggcgtt gcgcagcaac tcaatgggcg cgtcattctc accggcggcc ccgtggctct ttttgatctt gctggtcgca acgtgggccg gccggaaatc tgccaccgct gccgccatga tcagcgcttc ggccgcagga gcgtgtttgg tgacagcgtc ctgcaattgc gcggcggagc tgatatgcac cacctcgacg ccagcgggat ccggtaagcc gacggtatga cccgcgatga gcgtgacttc ggcaccacgc tgagcggcga cccgggcgat ggcgtatccc tgcttaccgg aactgcggtt tccgataaag cgcaccgggt ccaggggttc acgagtgccg ccggcggtga ccaggacccg cacaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggng gcgggagcga ccantaccag gtnggcatcc tgtccgagcc gcacgtgcgg aacctnggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggnggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggcctngtag gcggngattc ctccacccac tccgacgatg atccgggaca tcgaaccagg accctgggtc tgagcttcca gggtacccat a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIIVGVG GGIAAYKACT VVRQLAEAGH SVRVVPTESA LQFVGAATFE ALSGEPARTG VFDDVPEVPH VRLGQDADLV VVAPATADLL SRAATGRADD LLTATLLTAT CPVLFAPAMH TEMWLHPATQ QNVATLRSRG AVVLEPASGR LTGADTGPGR LPEAEEITTL AQLLLARPDA LPHDLAGVRV LVTAGGTREP LDPVRFIGNR SSGKQGYAIA RVAAQRGAEV TLIAGHTVGL PDPAGVEVVH ISSAAQLQDA VTKHAPAAEA LIMAAAVADF RPAHVATSKI KKSHGAAGEN DAPIELLRNA DVLAGVVRAR ADGQLPKMRA IVGFAAETGD ANGDVLFHAR AKLKRKGCDL LVVNAVGEGR AFEVDSNDGW LLSAQSGGDI TEVVLEHGTK VLMASRIVDA VAGLCQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCCGGATC ATCGTCGGAG TGGGTGGAGG AATCGCCGCC TACAAGGCCT GCACCGTGGT GCGCCAGCTC GCCGAGGCCG GGCACAGCGT CCGGGTCGTT CCCACCGAGT CGGCCCTGCA GTTCGTGGGA GCCGCGACCT TTGAGGCGCT CTCCGGTGAG CCGGCCCGTA CCGGGGTGTT CGACGATGTT CCCGAGGTTC CGCACGTGCG GCTCGGACAG GATGCCGACC TGGTAGTGGT CGCTCCCGCC ACCGCGGACC TGCTGTCGCG TGCCGCCACC GGCCGGGCGG ATGACCTGCT GACCGCGACC CTGCTCACCG CCACGTGTCC AGTGCTGTTC GCCCCCGCGA TGCATACGGA GATGTGGCTG CATCCGGCAA CCCAGCAGAA TGTCGCGACC CTGCGGAGCC GTGGCGCGGT TGTCCTGGAG CCCGCCTCTG GCCGCCTGAC GGGGGCGGAC ACCGGCCCGG GCCGTCTTCC CGAGGCCGAA GAAATCACCA CCCTGGCCCA ATTATTGCTG GCACGACCCG ACGCGCTGCC GCACGATCTG GCCGGTGTGC GGGTCCTGGT CACCGCCGGC GGCACTCGTG AACCCCTGGA CCCGGTGCGC TTTATCGGAA ACCGCAGTTC CGGTAAGCAG GGATACGCCA TCGCCCGGGT CGCCGCTCAG CGTGGTGCCG AAGTCACGCT CATCGCGGGT CATACCGTCG GCTTACCGGA TCCCGCTGGC GTCGAGGTGG TGCATATCAG CTCCGCCGCG CAATTGCAGG ACGCTGTCAC CAAACACGCT CCTGCGGCCG AAGCGCTGAT CATGGCGGCA GCGGTGGCAG ATTTCCGGCC GGCCCACGTT GCGACCAGCA AGATCAAAAA GAGCCACGGG GCCGCCGGTG AGAATGACGC GCCCATTGAG TTGCTGCGCA ACGCCGATGT GCTGGCCGGT GTGGTTCGTG CGCGTGCAGA CGGGCAGCTG CCCAAGATGC GGGCAATCGT CGGGTTTGCC GCGGAAACGG GCGACGCAAA CGGCGATGTG CTCTTTCACG CCCGCGCCAA GTTGAAGCGC AAGGGCTGTG ATTTGTTGGT AGTCAACGCT GTTGGCGAGG GACGGGCCTT CGAGGTGGAC AGCAATGACG GGTGGTTGCT CTCGGCGCAG AGTGGTGGTG ACATCACCGA AGTTGTCCTT GAACACGGCA CCAAAGTTCT GATGGCAAGC CGTATTGTGG ATGCCGTTGC CGGGCTTTGT CAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for MyabA.01254.a.A1.PW37463
PURIFICATION DATe: 3/19/2014
CONCENTRATION: 9.8mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSRIIVG VGGGIXAYXA CTVVRQLAEA GHSVRVVPXE SALQFVGAAT FEALSGEPAR TGVFDDVPXV PHVRLGQDAX LVXVAPAXAD LLSRAATGRA DDLLTATLLT ATCPVLFAPA MHTEMWLHPA TQQNVATLRS RGAVVLEPAS GRLTGADTGP GRLPEAEEIT TLAQLLLARP DALPHDLAGV RVLVTAGGTR EPLDPVRFIG NRSSGKQGYA IARVAAQRGA EVTLIAGHTV GLPDPAGVEV VHISSAAQLQ DAVTKHAPAA EALIMAAAVA DFRPAHVATS KIKKSHGAAG ENDAPIELLR NADVLAGVVR ARADGQLPKM RAIVGFAAET GDANGDVLFH ARAKLKRKGC DLLVVNAVGE GRAFEVDSND GWLLSAQSGG DITEVVLEHG TKVLMASRIV DAVAGLCQ
Validated NT Sequence
ttattactga caaagcccgg caacggcatc cacaatacgg cttgccatca gaactttggt gccgtgttca aggacaactt cggtgatgtc accaccactc tgcgccgaga gcaaccaccc gtcattgctg tccacctcga aggcccgtcc ctcgccaaca gcgttgacta ccaacaaatc acagcccttg cgcttcaact tggcgcgggc gtgaaagagc acatcgccgt ttgcgtcgcc cgtttccgcg gcaaacccga cgattgcccg catcttgggc agctgcccgt ctgcacgcgc acgaaccaca ccggccagca catcggcgtt gcgcagcaac tcaatgggcg cgtcattctc accggcggcc ccgtggctct ttttgatctt gctggtcgca acgtgggccg gccggaaatc tgccaccgct gccgccatga tcagcgcttc ggccgcagga gcgtgtttgg tgacagcgtc ctgcaattgc gcggcggagc tgatatgcac cacctcgacg ccagcgggat ccggtaagcc gacggtatga cccgcgatga gcgtgacttc ggcaccacgc tgagcggcga cccgggcgat ggcgtatccc tgcttaccgg aactgcggtt tccgataaag cgcaccgggt ccaggggttc acgagtgccg ccggcggtga ccaggacccg cacaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggng gcgggagcga ccantaccag gtnggcatcc tgtccgagcc gcacgtgcgg aacctnggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggnggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggcctngtag gcggngattc ctccacccac tccgacgatg atccgggaca tcgaaccagg accctgggtc tgagcttcca gggtacccat a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIIVGVG GGIAAYKACT VVRQLAEAGH SVRVVPTESA LQFVGAATFE ALSGEPARTG VFDDVPEVPH VRLGQDADLV VVAPATADLL SRAATGRADD LLTATLLTAT CPVLFAPAMH TEMWLHPATQ QNVATLRSRG AVVLEPASGR LTGADTGPGR LPEAEEITTL AQLLLARPDA LPHDLAGVRV LVTAGGTREP LDPVRFIGNR SSGKQGYAIA RVAAQRGAEV TLIAGHTVGL PDPAGVEVVH ISSAAQLQDA VTKHAPAAEA LIMAAAVADF RPAHVATSKI KKSHGAAGEN DAPIELLRNA DVLAGVVRAR ADGQLPKMRA IVGFAAETGD ANGDVLFHAR AKLKRKGCDL LVVNAVGEGR AFEVDSNDGW LLSAQSGGDI TEVVLEHGTK VLMASRIVDA VAGLCQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCCGGATC ATCGTCGGAG TGGGTGGAGG AATCGCCGCC TACAAGGCCT GCACCGTGGT GCGCCAGCTC GCCGAGGCCG GGCACAGCGT CCGGGTCGTT CCCACCGAGT CGGCCCTGCA GTTCGTGGGA GCCGCGACCT TTGAGGCGCT CTCCGGTGAG CCGGCCCGTA CCGGGGTGTT CGACGATGTT CCCGAGGTTC CGCACGTGCG GCTCGGACAG GATGCCGACC TGGTAGTGGT CGCTCCCGCC ACCGCGGACC TGCTGTCGCG TGCCGCCACC GGCCGGGCGG ATGACCTGCT GACCGCGACC CTGCTCACCG CCACGTGTCC AGTGCTGTTC GCCCCCGCGA TGCATACGGA GATGTGGCTG CATCCGGCAA CCCAGCAGAA TGTCGCGACC CTGCGGAGCC GTGGCGCGGT TGTCCTGGAG CCCGCCTCTG GCCGCCTGAC GGGGGCGGAC ACCGGCCCGG GCCGTCTTCC CGAGGCCGAA GAAATCACCA CCCTGGCCCA ATTATTGCTG GCACGACCCG ACGCGCTGCC GCACGATCTG GCCGGTGTGC GGGTCCTGGT CACCGCCGGC GGCACTCGTG AACCCCTGGA CCCGGTGCGC TTTATCGGAA ACCGCAGTTC CGGTAAGCAG GGATACGCCA TCGCCCGGGT CGCCGCTCAG CGTGGTGCCG AAGTCACGCT CATCGCGGGT CATACCGTCG GCTTACCGGA TCCCGCTGGC GTCGAGGTGG TGCATATCAG CTCCGCCGCG CAATTGCAGG ACGCTGTCAC CAAACACGCT CCTGCGGCCG AAGCGCTGAT CATGGCGGCA GCGGTGGCAG ATTTCCGGCC GGCCCACGTT GCGACCAGCA AGATCAAAAA GAGCCACGGG GCCGCCGGTG AGAATGACGC GCCCATTGAG TTGCTGCGCA ACGCCGATGT GCTGGCCGGT GTGGTTCGTG CGCGTGCAGA CGGGCAGCTG CCCAAGATGC GGGCAATCGT CGGGTTTGCC GCGGAAACGG GCGACGCAAA CGGCGATGTG CTCTTTCACG CCCGCGCCAA GTTGAAGCGC AAGGGCTGTG ATTTGTTGGT AGTCAACGCT GTTGGCGAGG GACGGGCCTT CGAGGTGGAC AGCAATGACG GGTGGTTGCT CTCGGCGCAG AGTGGTGGTG ACATCACCGA AGTTGTCCTT GAACACGGCA CCAAAGTTCT GATGGCAAGC CGTATTGTGG ATGCCGTTGC CGGGCTTTGT CAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for MyabA.01254.a.A1.PW37464
PURIFICATION DATe: 3/19/2014
CONCENTRATION: 25.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSRIIVG VGGGIXAYXA CTVVRQLAEA GHSVRVVPXE SALQFVGAAT FEALSGEPAR TGVFDDVPXV PHVRLGQDAX LVXVAPAXAD LLSRAATGRA DDLLTATLLT ATCPVLFAPA MHTEMWLHPA TQQNVATLRS RGAVVLEPAS GRLTGADTGP GRLPEAEEIT TLAQLLLARP DALPHDLAGV RVLVTAGGTR EPLDPVRFIG NRSSGKQGYA IARVAAQRGA EVTLIAGHTV GLPDPAGVEV VHISSAAQLQ DAVTKHAPAA EALIMAAAVA DFRPAHVATS KIKKSHGAAG ENDAPIELLR NADVLAGVVR ARADGQLPKM RAIVGFAAET GDANGDVLFH ARAKLKRKGC DLLVVNAVGE GRAFEVDSND GWLLSAQSGG DITEVVLEHG TKVLMASRIV DAVAGLCQ
Validated NT Sequence
ttattactga caaagcccgg caacggcatc cacaatacgg cttgccatca gaactttggt gccgtgttca aggacaactt cggtgatgtc accaccactc tgcgccgaga gcaaccaccc gtcattgctg tccacctcga aggcccgtcc ctcgccaaca gcgttgacta ccaacaaatc acagcccttg cgcttcaact tggcgcgggc gtgaaagagc acatcgccgt ttgcgtcgcc cgtttccgcg gcaaacccga cgattgcccg catcttgggc agctgcccgt ctgcacgcgc acgaaccaca ccggccagca catcggcgtt gcgcagcaac tcaatgggcg cgtcattctc accggcggcc ccgtggctct ttttgatctt gctggtcgca acgtgggccg gccggaaatc tgccaccgct gccgccatga tcagcgcttc ggccgcagga gcgtgtttgg tgacagcgtc ctgcaattgc gcggcggagc tgatatgcac cacctcgacg ccagcgggat ccggtaagcc gacggtatga cccgcgatga gcgtgacttc ggcaccacgc tgagcggcga cccgggcgat ggcgtatccc tgcttaccgg aactgcggtt tccgataaag cgcaccgggt ccaggggttc acgagtgccg ccggcggtga ccaggacccg cacaccggcc agatcgtgcg gcagcgcgtc gggtcgtgcc agcaataatt gggccagggt ggtgatttct tcggcctcgg gaagacggcc cgggccggtg tccgcccccg tcaggcggcc agaggcgggc tccaggacaa ccgcgccacg gctccgcagg gtcgcgacat tctgctgggt tgccggatgc agccacatct ccgtatgcat cgcgggggcg aacagcactg gacacgtggc ggtgagcagg gtcgcggtca gcaggtcatc cgcccggccg gtggcggcac gcgacagcag gtccgcggng gcgggagcga ccantaccag gtnggcatcc tgtccgagcc gcacgtgcgg aacctnggga acatcgtcga acaccccggt acgggccggc tcaccggaga gcgcctcaaa ggtcgcggct cccacgaact gcagggccga ctcggnggga acgacccgga cgctgtgccc ggcctcggcg agctggcgca ccacggtgca ggcctngtag gcggngattc ctccacccac tccgacgatg atccgggaca tcgaaccagg accctgggtc tgagcttcca gggtacccat a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIIVGVG GGIAAYKACT VVRQLAEAGH SVRVVPTESA LQFVGAATFE ALSGEPARTG VFDDVPEVPH VRLGQDADLV VVAPATADLL SRAATGRADD LLTATLLTAT CPVLFAPAMH TEMWLHPATQ QNVATLRSRG AVVLEPASGR LTGADTGPGR LPEAEEITTL AQLLLARPDA LPHDLAGVRV LVTAGGTREP LDPVRFIGNR SSGKQGYAIA RVAAQRGAEV TLIAGHTVGL PDPAGVEVVH ISSAAQLQDA VTKHAPAAEA LIMAAAVADF RPAHVATSKI KKSHGAAGEN DAPIELLRNA DVLAGVVRAR ADGQLPKMRA IVGFAAETGD ANGDVLFHAR AKLKRKGCDL LVVNAVGEGR AFEVDSNDGW LLSAQSGGDI TEVVLEHGTK VLMASRIVDA VAGLCQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCCGGATC ATCGTCGGAG TGGGTGGAGG AATCGCCGCC TACAAGGCCT GCACCGTGGT GCGCCAGCTC GCCGAGGCCG GGCACAGCGT CCGGGTCGTT CCCACCGAGT CGGCCCTGCA GTTCGTGGGA GCCGCGACCT TTGAGGCGCT CTCCGGTGAG CCGGCCCGTA CCGGGGTGTT CGACGATGTT CCCGAGGTTC CGCACGTGCG GCTCGGACAG GATGCCGACC TGGTAGTGGT CGCTCCCGCC ACCGCGGACC TGCTGTCGCG TGCCGCCACC GGCCGGGCGG ATGACCTGCT GACCGCGACC CTGCTCACCG CCACGTGTCC AGTGCTGTTC GCCCCCGCGA TGCATACGGA GATGTGGCTG CATCCGGCAA CCCAGCAGAA TGTCGCGACC CTGCGGAGCC GTGGCGCGGT TGTCCTGGAG CCCGCCTCTG GCCGCCTGAC GGGGGCGGAC ACCGGCCCGG GCCGTCTTCC CGAGGCCGAA GAAATCACCA CCCTGGCCCA ATTATTGCTG GCACGACCCG ACGCGCTGCC GCACGATCTG GCCGGTGTGC GGGTCCTGGT CACCGCCGGC GGCACTCGTG AACCCCTGGA CCCGGTGCGC TTTATCGGAA ACCGCAGTTC CGGTAAGCAG GGATACGCCA TCGCCCGGGT CGCCGCTCAG CGTGGTGCCG AAGTCACGCT CATCGCGGGT CATACCGTCG GCTTACCGGA TCCCGCTGGC GTCGAGGTGG TGCATATCAG CTCCGCCGCG CAATTGCAGG ACGCTGTCAC CAAACACGCT CCTGCGGCCG AAGCGCTGAT CATGGCGGCA GCGGTGGCAG ATTTCCGGCC GGCCCACGTT GCGACCAGCA AGATCAAAAA GAGCCACGGG GCCGCCGGTG AGAATGACGC GCCCATTGAG TTGCTGCGCA ACGCCGATGT GCTGGCCGGT GTGGTTCGTG CGCGTGCAGA CGGGCAGCTG CCCAAGATGC GGGCAATCGT CGGGTTTGCC GCGGAAACGG GCGACGCAAA CGGCGATGTG CTCTTTCACG CCCGCGCCAA GTTGAAGCGC AAGGGCTGTG ATTTGTTGGT AGTCAACGCT GTTGGCGAGG GACGGGCCTT CGAGGTGGAC AGCAATGACG GGTGGTTGCT CTCGGCGCAG AGTGGTGGTG ACATCACCGA AGTTGTCCTT GAACACGGCA CCAAAGTTCT GATGGCAAGC CGTATTGTGG ATGCCGTTGC CGGGCTTTGT CAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA