MyleA.01254.a

Putative flavoprotein

CENTER ID: MyleA.01254.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyleA.01254.a.B1.GE36889 Full length( MyleA.01254.a ) 1 419
MyleA.01254.a.B2.GE36890 Phosphopantothenoylcysteine decarboxylase 4 188
MyleA.01254.a.B3.GE36891 Phosphopantothenate-cysteine ligase 185 419
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127040
PATRIC ID: fig|561304.4.peg.939
RefSeq: YP_002503093.1
UniProt: A0A0H3MPP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDCKRVVVGV SGGIAAYKAC TVVRQLIDAG HEVRVIPTES ALRFVGTATF EALSGQPVYT DVFDDIPAVS HVYLGKQADL VVVAPATADL LARAVHGRAD DLLTATLLTA RCPIMFAPAM HTEMWLHPAT VDNVATLRRR GAIVLEPASG RLTGTDSGAG RLPEAEEITT LAQLLLERHD ALPYDLTGRK LLVTSGGTRE SIDPVRFIGN RSSGKQGYAV ARVAVQRGAE VTLIAGHTAG LTNPAGVDVV HVGSAQQLGD AVSKHAPDFD VLVMAAAVAD FRPAQVATAK IKKGPDDQDE PLIELVYNED VLAAAVRART HGQLPNMRAI VGFAAETGDA SGDVLFHARA KLRRKGCDLL VVNAVGDGRA FEVDNNDGWL LASDGTELAL QHGSKMLMAS RIVDGIVTFL HGLPDLRGG
NT Sequence
ATGGACTGCA AGCGGGTCGT TGTCGGCGTC TCCGGGGGTA TCGCCGCGTA TAAGGCGTGT ACCGTGGTTC GTCAGCTCAT TGACGCCGGT CACGAGGTGC GGGTCATCCC AACTGAGTCG GCGTTGCGCT TCGTCGGAAC TGCCACCTTC GAGGCACTCT CCGGCCAACC GGTGTACACC GACGTGTTCG ACGATATTCC GGCAGTGTCG CATGTGTACC TCGGCAAGCA GGCCGACTTG GTGGTAGTGG CGCCGGCCAC CGCCGACCTA TTGGCCCGCG CAGTGCACGG CCGAGCCGAC GATCTGTTAA CCGCGACCCT GCTCACCGCA CGGTGTCCGA TCATGTTCGC GCCGGCGATG CACACCGAGA TGTGGTTGCA TCCGGCTACC GTCGACAACG TGGCCACGTT GCGTCGCCGC GGCGCGATTG TTCTGGAACC CGCGTCCGGG CGCCTCACCG GTACCGATAG CGGAGCGGGA CGCCTGCCCG AGGCGGAAGA GATCACAACC CTTGCCCAGT TACTGCTGGA GCGGCACGAC GCTTTACCCT ATGACCTAAC GGGCAGAAAA CTTCTGGTGA CCTCCGGTGG TACCCGCGAG TCGATCGATC CGGTGCGCTT CATTGGGAAC CGCAGCTCCG GTAAGCAGGG CTATGCTGTC GCGCGGGTCG CTGTTCAGCG CGGCGCCGAA GTCACGTTGA TCGCTGGGCA CACCGCGGGG CTGACCAACC CTGCTGGGGT CGATGTGGTG CATGTTGGCT CGGCGCAGCA ACTCGGAGAC GCCGTTTCCA AGCACGCTCC CGACTTTGAC GTGTTGGTGA TGGCAGCGGC CGTGGCTGAT TTCCGGCCCG CCCAAGTTGC TACCGCCAAG ATCAAAAAAG GCCCCGATGA CCAGGACGAG CCGCTGATCG AGTTGGTGTA CAACGAAGAC GTGTTGGCTG CCGCGGTGCG GGCCCGTACG CATGGGCAGT TGCCCAACAT GCGGGCCATT GTGGGATTCG CCGCAGAAAC CGGGGATGCC AGCGGCGATG TGTTGTTTCA TGCTCGCGCT AAGCTGCGGC GCAAGGGCTG TGACCTTTTA GTCGTGAATG CTGTGGGTGA CGGCAGGGCC TTTGAGGTGG ACAACAACGA CGGCTGGCTG CTGGCGTCCG ACGGTACCGA GTTGGCGCTA CAGCACGGCT CCAAGATGCT CATGGCCAGT CGTATCGTTG ATGGGATTGT CACGTTTCTG CATGGCCTTC CCGATCTGCG AGGCGGG
Details for MyleA.01254.a.B1.GE36889
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMD CKRVVVGVSG GIAAYKACTV VRQLIDAGHE VRVIPTESAL RFVGTATFEA LSGQPVYTDV FDDIPAVSHV YLGKQADLVV VAPATADLLA RAVHGRADDL LTATLLTARC PIMFAPAMHT EMWLHPATVD NVATLRRRGA IVLEPASGRL TGTDSGAGRL PEAEEITTLA QLLLERHDAL PYDLTGRKLL VTSGGTRESI DPVRFIGNRS SGKQGYAVAR VAVQRGAEVT LIAGHTAGLT NPAGVDVVHV GSAXQLGDAV SKHAPDFDVL VMAAAVADFR PAQVATAKIK KGPDXQXXPL IELVYXEXVL AXAXRAXTHG XLPXXXXVGX XXKXXXXXMX XXCSXXLXXR XXXCXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
attttntnnn ntttaagang gagatatacc atggctcacc accaccacca ccatatggac tgcaagcggg tcgttgtcgg cgtctccggg ggtatcgccg cgtataaggc gtgtaccgtg gttcgtcagc tcattgacgc cggtcacgag gtgcgggtca tcccaactga gtcggcgttg cgcttcgtcg gaactgccac cttcgaggca ctctccggcc aaccggtgta caccgacgtg ttcgacgata ttccggcagt gtcgcatgtg tacctcggca agcaggccga cttggtggta gtggcgccgg ccaccgccga cctattggcc cgcgcagtgc acggccgagc cgacgatctg ttaaccgcga ccctgctcac cgcacggtgt ccgatcatgt tcgcgccggc gatgcacacc gagatgtggt tgcatccggc taccgtcgac aacgtggcca cgttgcgtcg ccgcggcgcg attgttctgg aacccgcgtc cgggcgcctc accggtaccg atagcggagc gggacgcctg cccgaggcgg aagagatcac aacccttgcc cagttactgc tggagcggca cgacgcttta ccctatgacc taacgggcag aaaacttctg gtgacctccg gtggtacccg cgagtcgatc gatccggtgc gcttcattgg gaaccgcagc tccggtaagc agggctatgc tgtcgcgcgg gtcgctgttc agcgcggcgc cgaagtcacg ttgatcgctg ggcacaccgc ggggctgacc aaccctgctg gggtcgatgt ggtgcatgtt ggctcggcgc ancaactcgg agacgccgtt tccaagcacg ctcccgactt tgacgtgttg gtgatggcag cggccgtggc tgatttccgg cccgcccaag ttgctaccgc caagatcaaa aaaggccccg atgancagna cnagccgctg atcgagttgg tgtacnacga anangtgttg gctgncgcgg ngcgggcccn tacgcatggg nagttgccna ntgnngnnnt tgtgggnntc nnnnnnaaan nngnnngcnn nnnnatgtnt nntnnntgct cgnnntanct gnnnnnnagg nngtgnnntt gcnnnngnnn nngnnnnnnt nnnnnnnnna nnnnannncn nnnnnnnnnn nnnnnnnntn nnnnnnnnnn aannnnnnnn nnnnncnnnt nnang
Expected Protein Sequence
MAHHHHHHMD CKRVVVGVSG GIAAYKACTV VRQLIDAGHE VRVIPTESAL RFVGTATFEA LSGQPVYTDV FDDIPAVSHV YLGKQADLVV VAPATADLLA RAVHGRADDL LTATLLTARC PIMFAPAMHT EMWLHPATVD NVATLRRRGA IVLEPASGRL TGTDSGAGRL PEAEEITTLA QLLLERHDAL PYDLTGRKLL VTSGGTRESI DPVRFIGNRS SGKQGYAVAR VAVQRGAEVT LIAGHTAGLT NPAGVDVVHV GSAQQLGDAV SKHAPDFDVL VMAAAVADFR PAQVATAKIK KGPDDQDEPL IELVYNEDVL AAAVRARTHG QLPNMRAIVG FAAETGDASG DVLFHARAKL RRKGCDLLVV NAVGDGRAFE VDNNDGWLLA SDGTELALQH GSKMLMASRI VDGIVTFLHG LPDLRGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggact gcaagcgggt cgttgtcggc gtctccgggg gtatcgccgc gtataaggcg tgtaccgtgg ttcgtcagct cattgacgcc ggtcacgagg tgcgggtcat cccaactgag tcggcgttgc gcttcgtcgg aactgccacc ttcgaggcac tctccggcca accggtgtac accgacgtgt tcgacgatat tccggcagtg tcgcatgtgt acctcggcaa gcaggccgac ttggtggtag tggcgccggc caccgccgac ctattggccc gcgcagtgca cggccgagcc gacgatctgt taaccgcgac cctgctcacc gcacggtgtc cgatcatgtt cgcgccggcg atgcacaccg agatgtggtt gcatccggct accgtcgaca acgtggccac gttgcgtcgc cgcggcgcga ttgttctgga acccgcgtcc gggcgcctca ccggtaccga tagcggagcg ggacgcctgc ccgaggcgga agagatcaca acccttgccc agttactgct ggagcggcac gacgctttac cctatgacct aacgggcaga aaacttctgg tgacctccgg tggtacccgc gagtcgatcg atccggtgcg cttcattggg aaccgcagct ccggtaagca gggctatgct gtcgcgcggg tcgctgttca gcgcggcgcc gaagtcacgt tgatcgctgg gcacaccgcg gggctgacca accctgctgg ggtcgatgtg gtgcatgttg gctcggcgca gcaactcgga gacgccgttt ccaagcacgc tcccgacttt gacgtgttgg tgatggcagc ggccgtggct gatttccggc ccgcccaagt tgctaccgcc aagatcaaaa aaggccccga tgaccaggac gagccgctga tcgagttggt gtacaacgaa gacgtgttgg ctgccgcggt gcgggcccgt acgcatgggc agttgcccaa catgcgggcc attgtgggat tcgccgcaga aaccggggat gccagcggcg atgtgttgtt tcatgctcgc gctaagctgc ggcgcaaggg ctgtgacctt ttagtcgtga atgctgtggg tgacggcagg gcctttgagg tggacaacaa cgacggctgg ctgctggcgt ccgacggtac cgagttggcg ctacagcacg gctccaagat gctcatggcc agtcgtatcg ttgatgggat tgtcacgttt ctgcatggcc ttcccgatct gcgaggcggg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MyleA.01254.a.B2.GE36890
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK RVVVGVSGGI AAYKACTVVR QLIDAGHEVR VIPTESALRF VGTATFEALS GQPVYTDVFD DIPAVSHVYL GKQADLVVVA PATADLLARA VHGRADDLLT ATLLTARCPI MFAPAMHTEM WLHPATVDNV ATLRRRGAIV LEPASGRLTG TDSGAGRLPE AEEITTLAQL LLERHDALPY DLTG
Validated NT Sequence
atcttactca cttagcccgt taggtcatag ggtaaagcgt cgtgccgctc cagcagtaac tgggcaaggg ttgtgatctc ttccgcctcg ggcaggcgtc ccgctccgct atcggtaccg gtgaggcgcc cggacgcggg ttccagaaca atcgcgccgc ggcgacgcaa cgtggccacg ttgtcgacgg tagccggatg caaccacatc tcggtgtgca tcgccggcgc gaacatgatc ggacaccgtg cggtgagcag ggtcgcggtt aacagatcgt cggctcggcc gtgcactgcg cgggccaata ggtcggcggt ggccggcgcc actaccacca agtcggcctg cttgccgagg tacacatgcg acactgccgg aatatcgtcg aacacgtcgg tgtacaccgg ttggccggag agtgcctcga aggtggcagt tccgacgaag cgcaacgccg actcagttgg gatgacccgc acctcgtgac cggcgtcaat gagctgacga accacggtac acgccttata cgcggcgata cccccggaga cgccgacaac gacccgcttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHKR VVVGVSGGIA AYKACTVVRQ LIDAGHEVRV IPTESALRFV GTATFEALSG QPVYTDVFDD IPAVSHVYLG KQADLVVVAP ATADLLARAV HGRADDLLTA TLLTARCPIM FAPAMHTEMW LHPATVDNVA TLRRRGAIVL EPASGRLTGT DSGAGRLPEA EEITTLAQLL LERHDALPYD LTG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagcggg tcgttgtcgg cgtctccggg ggtatcgccg cgtataaggc gtgtaccgtg gttcgtcagc tcattgacgc cggtcacgag gtgcgggtca tcccaactga gtcggcgttg cgcttcgtcg gaactgccac cttcgaggca ctctccggcc aaccggtgta caccgacgtg ttcgacgata ttccggcagt gtcgcatgtg tacctcggca agcaggccga cttggtggta gtggcgccgg ccaccgccga cctattggcc cgcgcagtgc acggccgagc cgacgatctg ttaaccgcga ccctgctcac cgcacggtgt ccgatcatgt tcgcgccggc gatgcacacc gagatgtggt tgcatccggc taccgtcgac aacgtggcca cgttgcgtcg ccgcggcgcg attgttctgg aacccgcgtc cgggcgcctc accggtaccg atagcggagc gggacgcctg cccgaggcgg aagagatcac aacccttgcc cagttactgc tggagcggca cgacgcttta ccctatgacc taacgggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MyleA.01254.a.B3.GE36891
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LTGRKLLVTS GGTRESIDPV RFIGNRSSGK QGYAVARVAV QRGAEVTLIA GHTAGLTNPA GVDVVHVGSA QQLGDAVSKH APDFDVLVMA AAVADFRPAQ VATAKIKKGP DDQDEPLIEL VYNEDVLAAA VRARTHGQLP NMRAIVGFAA ETGDASGDVL FHARAKLRRK GCDLLVVNAV GDGRAFEVDN NDGWLLASDG TELALQHGSX MLMASRIVDG IVTFLHGLPD LRGG
Validated NT Sequence
tttgttnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatggacct aacgggcaga aaacttctgg tgacctccgg tggtacccgc gagtcgatcg atccggtgcg cttcattggg aaccgcagct ccggtaagca gggctatgct gtcgcgcggg tcgctgttca gcgcggcgcc gaagtcacgt tgatcgctgg gcacaccgcg gggctgacca accctgctgg ggtcgatgtg gtgcatgttg gctcggcgca gcaactcgga gacgccgttt ccaagcacgc tcccgacttt gacgtgttgg tgatggcagc ggccgtggct gatttccggc ccgcccaagt tgctaccgcc aagatcaaaa aaggccccga tgaccaggac gagccgctga tcgagttggt gtacaacgaa gacgtgttgg ctgccgcggt gcgggcccgt acgcatgggc agttgcccaa catgcgggcc attgtgggat tcgccgcaga aaccggggat gccagcggcg atgtgttgtt tcatgctcgc gctaagctgc ggcgcaaggg ctgtgacctt ttagtcgtga atgctgtggg tgacggcagg gcctttgagg tggacaacaa cgacggctgg ctgctggcgt ccgacggtac cgagttggcg ctacagcacg gctccnagat gctcatggcc agtcgtatcg ttgatgggat tgtcacgttt ctgcatggcc ttcccgatct gcgaggcggg taagtgagta agataggatc cggctgcnnn nnncccccaa nnnnnnnnnn ancnttnnnn nnnnttnttt ntnctnntnn nnnnntnnnc
Expected Protein Sequence
MAHHHHHHDL TGRKLLVTSG GTRESIDPVR FIGNRSSGKQ GYAVARVAVQ RGAEVTLIAG HTAGLTNPAG VDVVHVGSAQ QLGDAVSKHA PDFDVLVMAA AVADFRPAQV ATAKIKKGPD DQDEPLIELV YNEDVLAAAV RARTHGQLPN MRAIVGFAAE TGDASGDVLF HARAKLRRKG CDLLVVNAVG DGRAFEVDNN DGWLLASDGT ELALQHGSKM LMASRIVDGI VTFLHGLPDL RGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacctaa cgggcagaaa acttctggtg acctccggtg gtacccgcga gtcgatcgat ccggtgcgct tcattgggaa ccgcagctcc ggtaagcagg gctatgctgt cgcgcgggtc gctgttcagc gcggcgccga agtcacgttg atcgctgggc acaccgcggg gctgaccaac cctgctgggg tcgatgtggt gcatgttggc tcggcgcagc aactcggaga cgccgtttcc aagcacgctc ccgactttga cgtgttggtg atggcagcgg ccgtggctga tttccggccc gcccaagttg ctaccgccaa gatcaaaaaa ggccccgatg accaggacga gccgctgatc gagttggtgt acaacgaaga cgtgttggct gccgcggtgc gggcccgtac gcatgggcag ttgcccaaca tgcgggccat tgtgggattc gccgcagaaa ccggggatgc cagcggcgat gtgttgtttc atgctcgcgc taagctgcgg cgcaagggct gtgacctttt agtcgtgaat gctgtgggtg acggcagggc ctttgaggtg gacaacaacg acggctggct gctggcgtcc gacggtaccg agttggcgct acagcacggc tccaagatgc tcatggccag tcgtatcgtt gatgggattg tcacgtttct gcatggcctt cccgatctgc gaggcgggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat