MymaA.01254.a

DNA/pantothenate metabolism flavoprotein, Dfp

CENTER ID: MymaA.01254.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MymaA.01254.a.A1.GE30161 Full length( MymaA.01254.a ) 1 413
MymaA.01254.a.B1.GE36892 Full length( MymaA.01254.a ) 1 413
MymaA.01254.a.B3.GE36894 Phosphopantothenate-cysteine ligase 185 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MymaA.01254.a.B1.PW37347 Full length( MymaA.01254.a ) 1 413
MymaA.01254.a.A1.PW37427 Full length( MymaA.01254.a ) 1 413

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2355
RefSeq: YP_001850508.1
UniProt: B2HP49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRKRIVVGV SGGIAAYKAC TVVRQLTEAG HHVRVIPTES ALRFIGAATF EALSGQPVHT GVFDDVPAVP HVQIGQQADL VVVAPATADL LARAVAGRGD DLLTATLLTA RCPVMFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPASG RLTGSDTGAG RLPEAEEITT LAQLLLERHD ALPYDLAGRK VLVTAGGTRE PIDPVRFVGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTTG LIDPAGVEVV HVSTAQQLDD AVSKHAPTAD VLVMAAAVAD FRPAQVSTAK IKKTADSPPV IELLRNDDVL AGAVRAREDG QLPKMRAIVG FAAETGDANG DVLFHARQKL RRKSCDLLVV NAVGGDRAFE VDNNDGWLLA ADGTEAALQH GSKTLMASRI VDAIVTFLDS CRG
NT Sequence
ATGGACCGTA AGCGGATCGT CGTCGGCGTC TCTGGGGGTA TCGCCGCGTA CAAGGCGTGC ACGGTCGTCC GTCAACTGAC TGAGGCCGGT CATCACGTCC GGGTCATCCC CACCGAATCT GCGCTCCGTT TTATCGGGGC GGCCACCTTC GAGGCGCTCT CCGGTCAGCC CGTGCACACC GGGGTGTTCG ACGACGTGCC GGCGGTGCCG CATGTTCAAA TCGGTCAGCA GGCTGACCTG GTCGTGGTCG CCCCGGCGAC GGCTGATCTG CTTGCCCGGG CGGTAGCCGG GCGCGGAGAC GACCTGCTGA CAGCAACTCT GCTCACGGCC CGGTGTCCGG TGATGTTCGC TCCAGCGATG CACACCGAGA TGTGGCTGCA TCCGGCCACC GTCGACAATG TGGCCACGCT GCGCCGTCGT GGCGCGGTGG TGCTCGAACC CGCATCCGGG CGGCTGACCG GCAGTGACAC CGGCGCCGGA CGCTTGCCCG AGGCCGAGGA GATCACCACC CTGGCCCAGC TGCTGCTGGA ACGCCATGAT GCGTTGCCCT ACGACCTGGC CGGCCGCAAG GTGTTGGTGA CCGCCGGTGG CACCCGGGAG CCGATCGATC CGGTGCGCTT CGTGGGCAAC CGCAGCTCCG GTAAGCAGGG TTACGCCGTC GCGCGCGTCG CTGCCCAGCG GGGCGCCGAG GTGACGTTGA TCGCGGGGCA CACCACCGGG TTGATCGACC CGGCCGGTGT CGAGGTGGTG CACGTCAGCA CCGCGCAGCA ACTCGATGAC GCCGTCTCCA AGCACGCACC CACCGCGGAC GTGCTGGTGA TGGCCGCCGC CGTTGCCGAC TTTCGGCCGG CGCAAGTTTC CACCGCCAAG ATCAAAAAGA CTGCCGACAG TCCACCGGTG ATCGAACTCC TGCGCAACGA CGATGTGTTG GCCGGTGCGG TGCGTGCGCG CGAGGATGGG CAGCTGCCCA AGATGCGTGC CATCGTTGGT TTTGCCGCCG AGACCGGTGA CGCCAACGGG GATGTGCTGT TTCACGCCCG ACAGAAGCTG CGGCGAAAAA GCTGCGACTT GCTGGTCGTC AACGCCGTGG GCGGTGACAG GGCCTTTGAG GTGGACAACA ACGACGGCTG GCTATTGGCC GCCGATGGAA CCGAGGCGGC GCTGCAGCAC GGGTCGAAGA CGCTGATGGC AAGCCGTATC GTTGACGCAA TAGTTACGTT CCTGGATAGC TGTCGCGGG
Details for MymaA.01254.a.A1.GE30161
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MDRKRIVVGV SGGIAAYKAC TVVRQLTEAG HHVRVIPTES ALRFIGAATF EALSGQPVHT GVFDDVPAVP HVQIGQQADL VVVAPATADL LARAVAGRGD DLLTATLLTA RCPVMFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPASG RLTGSDTGAG RLPEAEEITT LAQLLLERHD ALPYDLAGRK VLVTAGGTRE PIDPVRFVGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTTG LIDPAGVEVV HVSTAQQLDD AVSKHAPTAD VLVMAAAVAD FRPAQVSTAK IKKTADSPPV IELLRNDDVL AGAVRAREDG QLPKMRAIVG FAAETGDANG DVLFHARQKL RRKSCDLLVV NAVGGDRAFE VDNNDGWLLA ADGTEAALQH GSKTLMASRI VDAIVTFLDS CRG
Validated NT Sequence
ttattacccg cgacagctat ccaggaacgt aactattgcg tcaacgatac ggcttgccat cagcgtcttc gacccgtgct gcagcgccgc ctcggttcca tcggcggcca atagccagcc gtcgttgttg tccacctcaa aggccctgtc accgcccacg gcgttgacga ccagcaagtc gcagcttttt cgccgcagct tctgtcgggc gtgaaacagc acatccccgt tggcgtcacc ggtctcggcg gcaaaaccaa cgatggcacg catcttgggc agctgcccat cctcgcgcgc acgcaccgca ccggccaaca catcgtcgtt gcgcaggagt tcgatcaccg gtggactgtc ggcagtcttt ttgatcttgg cggtggaaac ttgcgccggc cgaaagtcgg caacggcggc ggccatcacc agcacgtccg cggtgggtgc gtgcttggag acggcgtcat cgagttgctg cgcggtgctg acgtgcacca cctcgacacc ggccgggtcg atcaacccgg tggtgtgccc cgcgatcaac gtcacctcgg cgccccgctg ggcagcgacg cgcgcgacgg cgtaaccctg cttaccggag ctgcggttgc ccacgaagcg caccggatcg atcggctccc gggtgccacc ggcggtcacc aacaccttgc ggccggccag gtcgtagggc aacgcatcat ggcgttccag cagcagctgg gccagggtgg tgatctcctc ggcctcgggc aagcgtccgg cgccggtgtc actgccggtc agccgcccgg atgcgggttc gagcaccacc gcgccacgac ggcgcagcgt ggccacattg tcgacggtgg ccggatgcag ccacatctcg gtgtgcatcg ctggagcgaa catcaccgga caccgggccg tgagcagagt tgctgtcagc aggtcgtctc cgcgcccggc taccgcccgg gcaagcagat cagccgtcgc cggggcgacc acgaccaggt cagcctgctg accgatttga acatgcggca ccgccggcac gtcgtcgaac accccggtgt gcacgggctg accggagagc gcctcgaagg tggccgcccc gataaaacgg agcgcagatt cggtggggat gacccggacg tgatgaccgg cctcagtcag ttgacggacg accgtgcacg ccttgtacgc ggcgataccc ccagagacgc cgacgacgat ccgcttacgg tccatcgaac caggacccng ggtntgagct tccagggtac cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRKRIVVG VSGGIAAYKA CTVVRQLTEA GHHVRVIPTE SALRFIGAAT FEALSGQPVH TGVFDDVPAV PHVQIGQQAD LVVVAPATAD LLARAVAGRG DDLLTATLLT ARCPVMFAPA MHTEMWLHPA TVDNVATLRR RGAVVLEPAS GRLTGSDTGA GRLPEAEEIT TLAQLLLERH DALPYDLAGR KVLVTAGGTR EPIDPVRFVG NRSSGKQGYA VARVAAQRGA EVTLIAGHTT GLIDPAGVEV VHVSTAQQLD DAVSKHAPTA DVLVMAAAVA DFRPAQVSTA KIKKTADSPP VIELLRNDDV LAGAVRARED GQLPKMRAIV GFAAETGDAN GDVLFHARQK LRRKSCDLLV VNAVGGDRAF EVDNNDGWLL AADGTEAALQ HGSKTLMASR IVDAIVTFLD SCRG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtaag cggatcgtcg tcggcgtctc tgggggtatc gccgcgtaca aggcgtgcac ggtcgtccgt caactgactg aggccggtca tcacgtccgg gtcatcccca ccgaatctgc gctccgtttt atcggggcgg ccaccttcga ggcgctctcc ggtcagcccg tgcacaccgg ggtgttcgac gacgtgccgg cggtgccgca tgttcaaatc ggtcagcagg ctgacctggt cgtggtcgcc ccggcgacgg ctgatctgct tgcccgggcg gtagccgggc gcggagacga cctgctgaca gcaactctgc tcacggcccg gtgtccggtg atgttcgctc cagcgatgca caccgagatg tggctgcatc cggccaccgt cgacaatgtg gccacgctgc gccgtcgtgg cgcggtggtg ctcgaacccg catccgggcg gctgaccggc agtgacaccg gcgccggacg cttgcccgag gccgaggaga tcaccaccct ggcccagctg ctgctggaac gccatgatgc gttgccctac gacctggccg gccgcaaggt gttggtgacc gccggtggca cccgggagcc gatcgatccg gtgcgcttcg tgggcaaccg cagctccggt aagcagggtt acgccgtcgc gcgcgtcgct gcccagcggg gcgccgaggt gacgttgatc gcggggcaca ccaccgggtt gatcgacccg gccggtgtcg aggtggtgca cgtcagcacc gcgcagcaac tcgatgacgc cgtctccaag cacgcaccca ccgcggacgt gctggtgatg gccgccgccg ttgccgactt tcggccggcg caagtttcca ccgccaagat caaaaagact gccgacagtc caccggtgat cgaactcctg cgcaacgacg atgtgttggc cggtgcggtg cgtgcgcgcg aggatgggca gctgcccaag atgcgtgcca tcgttggttt tgccgccgag accggtgacg ccaacgggga tgtgctgttt cacgcccgac agaagctgcg gcgaaaaagc tgcgacttgc tggtcgtcaa cgccgtgggc ggtgacaggg cctttgaggt ggacaacaac gacggctggc tattggccgc cgatggaacc gaggcggcgc tgcagcacgg gtcgaagacg ctgatggcaa gccgtatcgt tgacgcaata gttacgttcc tggatagctg tcgcgggaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MymaA.01254.a.B1.GE36892
HARVESTED ON: 5/1/2013
SEQUENCED ON: 3/3/2015
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMD RKRIVVGVSG GIAAYKACTV VRQLTEAGHH VRVIPTESAL RFIGAATFEA LSGQPVHTGV FDDVPAVPHV QIGQQADLVV VAPATADLLA RAVAGRGDDL LTATLLTARC PVMFAPAMHT EMWLHPATVD NVATLRRRGA VVLEPASGRL TGSDTGAGRL PEAEEITTLA QLLLERHDAL PYDLAGRKVL VTAGGTREPI DPVRFVGNRS SGKQGYAVAR VAAQRGAEVT LIAGHTTGLI DPAGVEVVHV STAQQLDDAV SKHAPTADVL VMAAAVADFR PAQVSTAKIK KTADSPPVIE LLRNDDVLAG AVRAREDGQL PKMRAIVGFA AETGDANGDV LFHARQKLRR KSCDLLVVNA VGGDRAFEVD NNDGWLLAAD GTEAALQHGS KTLMASRIVD
Validated NT Sequence
taccatggct caccaccacc accaccatat ggaccgtaag cggatcgtcg tcggcgtctc tgggggtatc gccgcgtaca aggcgtgcac ggtcgtccgt caactgactg aggccggtca tcacgtccgg gtcatcccca ccgaatctgc gctccgtttt atcggggcgg ccaccttcga ggcgctctcc ggtcagcccg tgcacaccgg ggtgttcgac gacgtgccgg cggtgccgca tgttcaaatc ggtcagcagg ctgacctggt cgtggtcgcc ccggcgacgg ctgatctgct tgcccgggcg gtagccgggc gcggagacga cctgctgaca gcaactctgc tcacggcccg gtgtccggtg atgttcgctc cagcgatgca caccgagatg tggctgcatc cggccaccgt cgacaatgtg gccacgctgc gccgtcgtgg cgcggtggtg ctcgaacccg catccgggcg gctgaccggc agtgacaccg gcgccggacg cttgcccgag gccgaggaga tcaccaccct ggcccagctg ctgctggaac gccatgatgc gttgccctac gacctggccg gccgcaaggt gttggtgacc gccggtggca cccgggagcc gatcgatccg gtgcgcttcg tgggcaaccg cagctccggt aagcagggtt acgccgtcgc gcgcgtcgct gcccagcggg gcgccgaggt gacgttgatc gcggggcaca ccaccgggtt gatcgacccg gccggtgtcg aggtggtgca cgtcagcacc gcgcagcaac tcgatgacgc cgtctccaag cacgcaccca ccgcggacgt gctggtgatg gccgccgccg ttgccgactt tcggccggcg caagtttcca ccgccaagat caaaaagact gccgacagtc caccggtgat cgaactcctg cgcaacgacg atgtgttggc cggtgcggtg cgtgcgcgcg aggatgggca gctgcccaag atgcgtgcca tcgttggttt tgccgccgag accggtgacg ccaacgggga tgtgctgttt cacgcccgac agaagctgcg gcgaaaaagc tgcgacttgc tggtcgtcaa cgccgtgggc ggtgacaggg cctttgaggt ggacaacaac gacggctggc tattggccgc cgatggaacc gaggcggcgc tgcagcacgg gtcgaagacg ctgatggcaa gccgtatcgt tgacgca
Expected Protein Sequence
MAHHHHHHMD RKRIVVGVSG GIAAYKACTV VRQLTEAGHH VRVIPTESAL RFIGAATFEA LSGQPVHTGV FDDVPAVPHV QIGQQADLVV VAPATADLLA RAVAGRGDDL LTATLLTARC PVMFAPAMHT EMWLHPATVD NVATLRRRGA VVLEPASGRL TGSDTGAGRL PEAEEITTLA QLLLERHDAL PYDLAGRKVL VTAGGTREPI DPVRFVGNRS SGKQGYAVAR VAAQRGAEVT LIAGHTTGLI DPAGVEVVHV STAQQLDDAV SKHAPTADVL VMAAAVADFR PAQVSTAKIK KTADSPPVIE LLRNDDVLAG AVRAREDGQL PKMRAIVGFA AETGDANGDV LFHARQKLRR KSCDLLVVNA VGGDRAFEVD NNDGWLLAAD GTEAALQHGS KTLMASRIVD AIVTFLDSCR G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacc gtaagcggat cgtcgtcggc gtctctgggg gtatcgccgc gtacaaggcg tgcacggtcg tccgtcaact gactgaggcc ggtcatcacg tccgggtcat ccccaccgaa tctgcgctcc gttttatcgg ggcggccacc ttcgaggcgc tctccggtca gcccgtgcac accggggtgt tcgacgacgt gccggcggtg ccgcatgttc aaatcggtca gcaggctgac ctggtcgtgg tcgccccggc gacggctgat ctgcttgccc gggcggtagc cgggcgcgga gacgacctgc tgacagcaac tctgctcacg gcccggtgtc cggtgatgtt cgctccagcg atgcacaccg agatgtggct gcatccggcc accgtcgaca atgtggccac gctgcgccgt cgtggcgcgg tggtgctcga acccgcatcc gggcggctga ccggcagtga caccggcgcc ggacgcttgc ccgaggccga ggagatcacc accctggccc agctgctgct ggaacgccat gatgcgttgc cctacgacct ggccggccgc aaggtgttgg tgaccgccgg tggcacccgg gagccgatcg atccggtgcg cttcgtgggc aaccgcagct ccggtaagca gggttacgcc gtcgcgcgcg tcgctgccca gcggggcgcc gaggtgacgt tgatcgcggg gcacaccacc gggttgatcg acccggccgg tgtcgaggtg gtgcacgtca gcaccgcgca gcaactcgat gacgccgtct ccaagcacgc acccaccgcg gacgtgctgg tgatggccgc cgccgttgcc gactttcggc cggcgcaagt ttccaccgcc aagatcaaaa agactgccga cagtccaccg gtgatcgaac tcctgcgcaa cgacgatgtg ttggccggtg cggtgcgtgc gcgcgaggat gggcagctgc ccaagatgcg tgccatcgtt ggttttgccg ccgagaccgg tgacgccaac ggggatgtgc tgtttcacgc ccgacagaag ctgcggcgaa aaagctgcga cttgctggtc gtcaacgccg tgggcggtga cagggccttt gaggtggaca acaacgacgg ctggctattg gccgccgatg gaaccgaggc ggcgctgcag cacgggtcga agacgctgat ggcaagccgt atcgttgacg caatagttac gttcctggat agctgtcgcg ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MymaA.01254.a.B3.GE36894
HARVESTED ON: 5/1/2013
SEQUENCED ON: 3/3/2015
EXPECTED MW: 25kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LAGRKVLVTA GGTREPIDPV RFVGNRSSGK QGYAVARVAA QRGAEVTLIA GHTTGLIDPA GVEVVHVSTA QQLDDAVSKH APTADVLVMA AAVADFRPAQ VSTAKIKKTA DSPPVIELLR NDDVLAGAVR AREDGQLPKM RAIVGFAAET GDANGDVLFH ARQKLRRKSC DLLVVNAVGG DRAFEVDNND GWLLAADGTE AALQHGSKTL MASRIVDAIV TFLDSCRG
Validated NT Sequence
atggctcacc accaccacca ccatatggac ctggccggcc gcaaggtgtt ggtgaccgcc ggtggcaccc gggagccgat cgatccggtg cgcttcgtgg gcaaccgcag ctccggtaag cagggttacg ccgtcgcgcg cgtcgctgcc cagcggggcg ccgaggtgac gttgatcgcg gggcacacca ccgggttgat cgacccggcc ggtgtcgagg tggtgcacgt cagcaccgcg cagcaactcg atgacgccgt ctccaagcac gcacccaccg cggacgtgct ggtgatggcc gccgccgttg ccgactttcg gccggcgcaa gtttccaccg ccaagatcaa aaagactgcc gacagtccac cggtgatcga actcctgcgc aacgacgatg tgttggccgg tgcggtgcgt gcgcgcgagg atgggcagct gcccaagatg cgtgccatcg ttggttttgc cgccgagacc ggtgacgcca acggggatgt gctgtttcac gcccgacaga agctgcggcg aaaaagctgc gacttgctgg tcgtcaacgc cgtgggcggt gacagggcct ttgaggtgga caacaacgac ggctggctat tggccgccga tggaaccgag gcggcgctgc agcacgggtc gaagacgctg atggcaagcc gtatcgttga cgcaatagtt acgttcctgg atagctgtcg cgggtaagtg agtaagat
Expected Protein Sequence
MAHHHHHHDL AGRKVLVTAG GTREPIDPVR FVGNRSSGKQ GYAVARVAAQ RGAEVTLIAG HTTGLIDPAG VEVVHVSTAQ QLDDAVSKHA PTADVLVMAA AVADFRPAQV STAKIKKTAD SPPVIELLRN DDVLAGAVRA REDGQLPKMR AIVGFAAETG DANGDVLFHA RQKLRRKSCD LLVVNAVGGD RAFEVDNNDG WLLAADGTEA ALQHGSKTLM ASRIVDAIVT FLDSCRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacctgg ccggccgcaa ggtgttggtg accgccggtg gcacccggga gccgatcgat ccggtgcgct tcgtgggcaa ccgcagctcc ggtaagcagg gttacgccgt cgcgcgcgtc gctgcccagc ggggcgccga ggtgacgttg atcgcggggc acaccaccgg gttgatcgac ccggccggtg tcgaggtggt gcacgtcagc accgcgcagc aactcgatga cgccgtctcc aagcacgcac ccaccgcgga cgtgctggtg atggccgccg ccgttgccga ctttcggccg gcgcaagttt ccaccgccaa gatcaaaaag actgccgaca gtccaccggt gatcgaactc ctgcgcaacg acgatgtgtt ggccggtgcg gtgcgtgcgc gcgaggatgg gcagctgccc aagatgcgtg ccatcgttgg ttttgccgcc gagaccggtg acgccaacgg ggatgtgctg tttcacgccc gacagaagct gcggcgaaaa agctgcgact tgctggtcgt caacgccgtg ggcggtgaca gggcctttga ggtggacaac aacgacggct ggctattggc cgccgatgga accgaggcgg cgctgcagca cgggtcgaag acgctgatgg caagccgtat cgttgacgca atagttacgt tcctggatag ctgtcgcggg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MymaA.01254.a.B1.PW37347
PURIFICATION DATe: 8/26/2013
CONCENTRATION: 25.6mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD RKRIVVGVSG GIAAYKACTV VRQLTEAGHH VRVIPTESAL RFIGAATFEA LSGQPVHTGV FDDVPAVPHV QIGQQADLVV VAPATADLLA RAVAGRGDDL LTATLLTARC PVMFAPAMHT EMWLHPATVD NVATLRRRGA VVLEPASGRL TGSDTGAGRL PEAEEITTLA QLLLERHDAL PYDLAGRKVL VTAGGTREPI DPVRFVGNRS SGKQGYAVAR VAAQRGAEVT LIAGHTTGLI DPAGVEVVHV STAQQLDDAV SKHAPTADVL VMAAAVADFR PAQVSTAKIK KTADSPPVIE LLRNDDVLAG AVRAREDGQL PKMRAIVGFA AETGDANGDV LFHARQKLRR KSCDLLVVNA VGGDRAFEVD NNDGWLLAAD GTEAALQHGS KTLMASRIVD
Validated NT Sequence
taccatggct caccaccacc accaccatat ggaccgtaag cggatcgtcg tcggcgtctc tgggggtatc gccgcgtaca aggcgtgcac ggtcgtccgt caactgactg aggccggtca tcacgtccgg gtcatcccca ccgaatctgc gctccgtttt atcggggcgg ccaccttcga ggcgctctcc ggtcagcccg tgcacaccgg ggtgttcgac gacgtgccgg cggtgccgca tgttcaaatc ggtcagcagg ctgacctggt cgtggtcgcc ccggcgacgg ctgatctgct tgcccgggcg gtagccgggc gcggagacga cctgctgaca gcaactctgc tcacggcccg gtgtccggtg atgttcgctc cagcgatgca caccgagatg tggctgcatc cggccaccgt cgacaatgtg gccacgctgc gccgtcgtgg cgcggtggtg ctcgaacccg catccgggcg gctgaccggc agtgacaccg gcgccggacg cttgcccgag gccgaggaga tcaccaccct ggcccagctg ctgctggaac gccatgatgc gttgccctac gacctggccg gccgcaaggt gttggtgacc gccggtggca cccgggagcc gatcgatccg gtgcgcttcg tgggcaaccg cagctccggt aagcagggtt acgccgtcgc gcgcgtcgct gcccagcggg gcgccgaggt gacgttgatc gcggggcaca ccaccgggtt gatcgacccg gccggtgtcg aggtggtgca cgtcagcacc gcgcagcaac tcgatgacgc cgtctccaag cacgcaccca ccgcggacgt gctggtgatg gccgccgccg ttgccgactt tcggccggcg caagtttcca ccgccaagat caaaaagact gccgacagtc caccggtgat cgaactcctg cgcaacgacg atgtgttggc cggtgcggtg cgtgcgcgcg aggatgggca gctgcccaag atgcgtgcca tcgttggttt tgccgccgag accggtgacg ccaacgggga tgtgctgttt cacgcccgac agaagctgcg gcgaaaaagc tgcgacttgc tggtcgtcaa cgccgtgggc ggtgacaggg cctttgaggt ggacaacaac gacggctggc tattggccgc cgatggaacc gaggcggcgc tgcagcacgg gtcgaagacg ctgatggcaa gccgtatcgt tgacgca
Expressed Protein Sequence
MAHHHHHHMD RKRIVVGVSG GIAAYKACTV VRQLTEAGHH VRVIPTESAL RFIGAATFEA LSGQPVHTGV FDDVPAVPHV QIGQQADLVV VAPATADLLA RAVAGRGDDL LTATLLTARC PVMFAPAMHT EMWLHPATVD NVATLRRRGA VVLEPASGRL TGSDTGAGRL PEAEEITTLA QLLLERHDAL PYDLAGRKVL VTAGGTREPI DPVRFVGNRS SGKQGYAVAR VAAQRGAEVT LIAGHTTGLI DPAGVEVVHV STAQQLDDAV SKHAPTADVL VMAAAVADFR PAQVSTAKIK KTADSPPVIE LLRNDDVLAG AVRAREDGQL PKMRAIVGFA AETGDANGDV LFHARQKLRR KSCDLLVVNA VGGDRAFEVD NNDGWLLAAD GTEAALQHGS KTLMASRIVD AIVTFLDSCR G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACC GTAAGCGGAT CGTCGTCGGC GTCTCTGGGG GTATCGCCGC GTACAAGGCG TGCACGGTCG TCCGTCAACT GACTGAGGCC GGTCATCACG TCCGGGTCAT CCCCACCGAA TCTGCGCTCC GTTTTATCGG GGCGGCCACC TTCGAGGCGC TCTCCGGTCA GCCCGTGCAC ACCGGGGTGT TCGACGACGT GCCGGCGGTG CCGCATGTTC AAATCGGTCA GCAGGCTGAC CTGGTCGTGG TCGCCCCGGC GACGGCTGAT CTGCTTGCCC GGGCGGTAGC CGGGCGCGGA GACGACCTGC TGACAGCAAC TCTGCTCACG GCCCGGTGTC CGGTGATGTT CGCTCCAGCG ATGCACACCG AGATGTGGCT GCATCCGGCC ACCGTCGACA ATGTGGCCAC GCTGCGCCGT CGTGGCGCGG TGGTGCTCGA ACCCGCATCC GGGCGGCTGA CCGGCAGTGA CACCGGCGCC GGACGCTTGC CCGAGGCCGA GGAGATCACC ACCCTGGCCC AGCTGCTGCT GGAACGCCAT GATGCGTTGC CCTACGACCT GGCCGGCCGC AAGGTGTTGG TGACCGCCGG TGGCACCCGG GAGCCGATCG ATCCGGTGCG CTTCGTGGGC AACCGCAGCT CCGGTAAGCA GGGTTACGCC GTCGCGCGCG TCGCTGCCCA GCGGGGCGCC GAGGTGACGT TGATCGCGGG GCACACCACC GGGTTGATCG ACCCGGCCGG TGTCGAGGTG GTGCACGTCA GCACCGCGCA GCAACTCGAT GACGCCGTCT CCAAGCACGC ACCCACCGCG GACGTGCTGG TGATGGCCGC CGCCGTTGCC GACTTTCGGC CGGCGCAAGT TTCCACCGCC AAGATCAAAA AGACTGCCGA CAGTCCACCG GTGATCGAAC TCCTGCGCAA CGACGATGTG TTGGCCGGTG CGGTGCGTGC GCGCGAGGAT GGGCAGCTGC CCAAGATGCG TGCCATCGTT GGTTTTGCCG CCGAGACCGG TGACGCCAAC GGGGATGTGC TGTTTCACGC CCGACAGAAG CTGCGGCGAA AAAGCTGCGA CTTGCTGGTC GTCAACGCCG TGGGCGGTGA CAGGGCCTTT GAGGTGGACA ACAACGACGG CTGGCTATTG GCCGCCGATG GAACCGAGGC GGCGCTGCAG CACGGGTCGA AGACGCTGAT GGCAAGCCGT ATCGTTGACG CAATAGTTAC GTTCCTGGAT AGCTGTCGCG GGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for MymaA.01254.a.A1.PW37427
PURIFICATION DATe: 1/22/2014
CONCENTRATION: 25.9mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MDRKRIVVGV SGGIAAYKAC TVVRQLTEAG HHVRVIPTES ALRFIGAATF EALSGQPVHT GVFDDVPAVP HVQIGQQADL VVVAPATADL LARAVAGRGD DLLTATLLTA RCPVMFAPAM HTEMWLHPAT VDNVATLRRR GAVVLEPASG RLTGSDTGAG RLPEAEEITT LAQLLLERHD ALPYDLAGRK VLVTAGGTRE PIDPVRFVGN RSSGKQGYAV ARVAAQRGAE VTLIAGHTTG LIDPAGVEVV HVSTAQQLDD AVSKHAPTAD VLVMAAAVAD FRPAQVSTAK IKKTADSPPV IELLRNDDVL AGAVRAREDG QLPKMRAIVG FAAETGDANG DVLFHARQKL RRKSCDLLVV NAVGGDRAFE VDNNDGWLLA ADGTEAALQH GSKTLMASRI VDAIVTFLDS CRG
Validated NT Sequence
ttattacccg cgacagctat ccaggaacgt aactattgcg tcaacgatac ggcttgccat cagcgtcttc gacccgtgct gcagcgccgc ctcggttcca tcggcggcca atagccagcc gtcgttgttg tccacctcaa aggccctgtc accgcccacg gcgttgacga ccagcaagtc gcagcttttt cgccgcagct tctgtcgggc gtgaaacagc acatccccgt tggcgtcacc ggtctcggcg gcaaaaccaa cgatggcacg catcttgggc agctgcccat cctcgcgcgc acgcaccgca ccggccaaca catcgtcgtt gcgcaggagt tcgatcaccg gtggactgtc ggcagtcttt ttgatcttgg cggtggaaac ttgcgccggc cgaaagtcgg caacggcggc ggccatcacc agcacgtccg cggtgggtgc gtgcttggag acggcgtcat cgagttgctg cgcggtgctg acgtgcacca cctcgacacc ggccgggtcg atcaacccgg tggtgtgccc cgcgatcaac gtcacctcgg cgccccgctg ggcagcgacg cgcgcgacgg cgtaaccctg cttaccggag ctgcggttgc ccacgaagcg caccggatcg atcggctccc gggtgccacc ggcggtcacc aacaccttgc ggccggccag gtcgtagggc aacgcatcat ggcgttccag cagcagctgg gccagggtgg tgatctcctc ggcctcgggc aagcgtccgg cgccggtgtc actgccggtc agccgcccgg atgcgggttc gagcaccacc gcgccacgac ggcgcagcgt ggccacattg tcgacggtgg ccggatgcag ccacatctcg gtgtgcatcg ctggagcgaa catcaccgga caccgggccg tgagcagagt tgctgtcagc aggtcgtctc cgcgcccggc taccgcccgg gcaagcagat cagccgtcgc cggggcgacc acgaccaggt cagcctgctg accgatttga acatgcggca ccgccggcac gtcgtcgaac accccggtgt gcacgggctg accggagagc gcctcgaagg tggccgcccc gataaaacgg agcgcagatt cggtggggat gacccggacg tgatgaccgg cctcagtcag ttgacggacg accgtgcacg ccttgtacgc ggcgataccc ccagagacgc cgacgacgat ccgcttacgg tccatcgaac caggacccng ggtntgagct tccagggtac cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRKRIVVG VSGGIAAYKA CTVVRQLTEA GHHVRVIPTE SALRFIGAAT FEALSGQPVH TGVFDDVPAV PHVQIGQQAD LVVVAPATAD LLARAVAGRG DDLLTATLLT ARCPVMFAPA MHTEMWLHPA TVDNVATLRR RGAVVLEPAS GRLTGSDTGA GRLPEAEEIT TLAQLLLERH DALPYDLAGR KVLVTAGGTR EPIDPVRFVG NRSSGKQGYA VARVAAQRGA EVTLIAGHTT GLIDPAGVEV VHVSTAQQLD DAVSKHAPTA DVLVMAAAVA DFRPAQVSTA KIKKTADSPP VIELLRNDDV LAGAVRARED GQLPKMRAIV GFAAETGDAN GDVLFHARQK LRRKSCDLLV VNAVGGDRAF EVDNNDGWLL AADGTEAALQ HGSKTLMASR IVDAIVTFLD SCRG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCGTAAG CGGATCGTCG TCGGCGTCTC TGGGGGTATC GCCGCGTACA AGGCGTGCAC GGTCGTCCGT CAACTGACTG AGGCCGGTCA TCACGTCCGG GTCATCCCCA CCGAATCTGC GCTCCGTTTT ATCGGGGCGG CCACCTTCGA GGCGCTCTCC GGTCAGCCCG TGCACACCGG GGTGTTCGAC GACGTGCCGG CGGTGCCGCA TGTTCAAATC GGTCAGCAGG CTGACCTGGT CGTGGTCGCC CCGGCGACGG CTGATCTGCT TGCCCGGGCG GTAGCCGGGC GCGGAGACGA CCTGCTGACA GCAACTCTGC TCACGGCCCG GTGTCCGGTG ATGTTCGCTC CAGCGATGCA CACCGAGATG TGGCTGCATC CGGCCACCGT CGACAATGTG GCCACGCTGC GCCGTCGTGG CGCGGTGGTG CTCGAACCCG CATCCGGGCG GCTGACCGGC AGTGACACCG GCGCCGGACG CTTGCCCGAG GCCGAGGAGA TCACCACCCT GGCCCAGCTG CTGCTGGAAC GCCATGATGC GTTGCCCTAC GACCTGGCCG GCCGCAAGGT GTTGGTGACC GCCGGTGGCA CCCGGGAGCC GATCGATCCG GTGCGCTTCG TGGGCAACCG CAGCTCCGGT AAGCAGGGTT ACGCCGTCGC GCGCGTCGCT GCCCAGCGGG GCGCCGAGGT GACGTTGATC GCGGGGCACA CCACCGGGTT GATCGACCCG GCCGGTGTCG AGGTGGTGCA CGTCAGCACC GCGCAGCAAC TCGATGACGC CGTCTCCAAG CACGCACCCA CCGCGGACGT GCTGGTGATG GCCGCCGCCG TTGCCGACTT TCGGCCGGCG CAAGTTTCCA CCGCCAAGAT CAAAAAGACT GCCGACAGTC CACCGGTGAT CGAACTCCTG CGCAACGACG ATGTGTTGGC CGGTGCGGTG CGTGCGCGCG AGGATGGGCA GCTGCCCAAG ATGCGTGCCA TCGTTGGTTT TGCCGCCGAG ACCGGTGACG CCAACGGGGA TGTGCTGTTT CACGCCCGAC AGAAGCTGCG GCGAAAAAGC TGCGACTTGC TGGTCGTCAA CGCCGTGGGC GGTGACAGGG CCTTTGAGGT GGACAACAAC GACGGCTGGC TATTGGCCGC CGATGGAACC GAGGCGGCGC TGCAGCACGG GTCGAAGACG CTGATGGCAA GCCGTATCGT TGACGCAATA GTTACGTTCC TGGATAGCTG TCGCGGGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA