MytuD.00340.a

Enoyl-CoA hydratase

CENTER ID: MytuD.00340.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00340.a.A1.GE25829 Full length( MytuD.00340.a ) 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00340.a.A1.PW25878 Full length( MytuD.00340.a ) 1 345

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1197
RefSeq: NP_215587.1
TubercuList: Rv1071c
MTB Network Portal: Rv1071c
UniProt: O53419

Orthologues for Rv1071c

Species % Identity % Coverage Clones Proteins Structures
marinum 82 100 0 0 0
ulcerans 82 100 1 1 0
avium 80 100 1 1 0
paratuberculosis 80 100 0 0 0
leprae 78 100 1 2 0
thermoresistibile 72 100 1 1 1
abscessus 70 99 1 1 0
smegmatis 68 99 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGESHEVLT NVEGGVGFVT LNRPKAINSL NQTMVDLLAT VLMSWEHEDA VHAVVLSGAG ERGLCAGGDV VAVYHSARKD GVEARRFWRH EYLLNALIGR FAKPYVALMD GIVMGGGVGV SAHANTRVVT DTSKVAMPEV GIGFIPDVGG VYLLSRAPGA LGLHAALTGA PFSGADAIAL GFADHFVPHG DLDAFTQKIV TGGVESALAA HAVEPPPSTL AAQRDWIDEC YAGDSVADIV AALRKQGGEP AVNASDLIAS RSPIALSVTL QAVRRAAKLD TLEDVLIQDY RVSSASLRSH DLVEGIRAQL IDKDRNPNWS PATLDAITAA DIEAYFEPVD DDLSF
NT Sequence
GTGACCGGCG AATCGCATGA GGTCCTGACA AACGTCGAGG GCGGTGTCGG ATTCGTGACG CTCAACCGCC CCAAGGCGAT CAACTCACTG AACCAAACCA TGGTCGACCT GCTGGCCACA GTGCTCATGA GCTGGGAGCA TGAGGACGCG GTGCACGCGG TGGTGCTCTC CGGAGCCGGC GAACGCGGAC TTTGCGCCGG CGGGGACGTG GTGGCCGTCT ACCACAGTGC CCGCAAGGAC GGGGTCGAGG CGCGGCGGTT CTGGCGCCAC GAGTATCTGC TCAACGCCCT GATCGGCCGG TTCGCCAAGC CCTACGTGGC GTTGATGGAC GGCATCGTAA TGGGCGGCGG CGTCGGCGTC AGCGCACACG CGAACACCCG GGTGGTTACC GATACCTCCA AGGTCGCGAT GCCCGAAGTG GGCATCGGGT TCATCCCCGA CGTCGGCGGG GTGTATTTGC TGTCGCGTGC ACCCGGCGCG CTGGGTCTGC ACGCCGCCCT GACTGGAGCG CCGTTTTCCG GCGCCGACGC CATCGCGCTG GGATTCGCCG ACCACTTCGT GCCACACGGC GACCTCGATG CGTTCACGCA GAAGATCGTC ACCGGCGGCG TGGAGAGCGC ATTGGCCGCC CACGCCGTCG AACCTCCACC GAGCACGCTT GCCGCCCAAC GTGATTGGAT CGACGAATGC TATGCCGGCG ACAGCGTCGC CGACATCGTT GCGGCGCTGC GGAAACAGGG CGGCGAACCA GCCGTAAATG CTTCCGACCT GATTGCCAGC CGCTCCCCCA TCGCGCTGTC GGTGACGTTG CAGGCAGTGC GTCGCGCCGC CAAACTCGAC ACGCTAGAAG ACGTGTTGAT CCAGGACTAT CGGGTGTCAT CGGCGTCGCT GCGCTCGCAT GACCTGGTGG AGGGCATCCG CGCGCAGTTG ATCGACAAGG ATCGCAACCC GAACTGGTCG CCGGCAACCC TGGACGCGAT CACGGCGGCC GACATCGAAG CCTATTTCGA ACCGGTCGAC GATGACTTGA GTTTC
Details for MytuD.00340.a.A1.GE25829
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 38kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTGESHEV LTNVEGGVGF VTLNRPKAIN SLNQTMVDLL ATVLMSWEHE DAVHAVVLSG AGERGLCAGG DVVAVYHSAR KDGVEARRFW RHEYLLNALI GRFAKPYVAL MDGIVMGGGV GVSAHANTRV VTDTSKVAMP EVGIGFIPDV GGVYLLSRAP GALGLHAALT GAPFSGADAI ALGFADHFVP HGDLDAFTQK IVTGGVESAL AAHAVEPPPS TLAAQRDWID ECYAGDSVAD IVAALRKQGG EPAVNASDLI ASRSPIALSV TLQAVRRAAK LDTLEDVLIQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPN WSPATLDAIT AADIEAYFEP VDDDLSF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ccggcgaatc gcatgaggtc ctgacaaacg tcgagggcgg tgtcggattc gtgacgctca accgccccaa ggcgatcaac tcactgaacc aaaccatggt cgacctgctg gccacagtgc tcatgagctg ggagcatgag gacgcggtgc acgcggtggt gctctccgga gccggcgaac gcggactttg cgccggcggg gacgtggtgg ccgtctacca cagtgcccgc aaggacgggg tcgaggcgcg gcggttctgg cgccacgagt atctgctcaa cgccctgatc ggccggttcg ccaagcccta cgtggcgttg atggacggca tcgtaatggg cggcggcgtc ggcgtcagcg cacacgcgaa cacccgggtg gttaccgata cctccaaggt cgcgatgccc gaagtgggca tcgggttcat ccccgacgtc ggcggggtgt atttgctgtc gcgtgcaccc ggcgcgctgg gtctgcacgc cgccctgact ggagcgccgt tttccggcgc cgacgccatc gcgctgggat tcgccgacca cttcgtgcca cacggcgacc tcgatgcgtt cacgcagaag atcgtcaccg gcggcgtgga gagcgcattg gccgcccacg ccgtcgaacc tccaccgagc acgcttgccg cccaacgtga ttggatcgac gaatgctatg ccggcgacag cgtcgccgac atcgttgcgg cgctgcggaa acagggcggc gaaccagccg taaatgcttc cgacctgatt gccagccgct cccccatcgc gctgtcggtg acgttgcagg cagtgcgtcg cgccgccaaa ctcgacacgc tagaagacgt gttgatccag gactatcggg tgtcatcggc gtcgctgcgc tcgcatgacc tggtggaggg catccgcgcg cagttgatcg acaaggatcg caacccgaac tggtcgccgg caaccctgga cgcgatcacg gcggccgaca tcgaagccta tttcgaaccg gtcgacgatg acttgagttt ctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTGESHEV LTNVEGGVGF VTLNRPKAIN SLNQTMVDLL ATVLMSWEHE DAVHAVVLSG AGERGLCAGG DVVAVYHSAR KDGVEARRFW RHEYLLNALI GRFAKPYVAL MDGIVMGGGV GVSAHANTRV VTDTSKVAMP EVGIGFIPDV GGVYLLSRAP GALGLHAALT GAPFSGADAI ALGFADHFVP HGDLDAFTQK IVTGGVESAL AAHAVEPPPS TLAAQRDWID ECYAGDSVAD IVAALRKQGG EPAVNASDLI ASRSPIALSV TLQAVRRAAK LDTLEDVLIQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPN WSPATLDAIT AADIEAYFEP VDDDLSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccggc gaatcgcatg aggtcctgac aaacgtcgag ggcggtgtcg gattcgtgac gctcaaccgc cccaaggcga tcaactcact gaaccaaacc atggtcgacc tgctggccac agtgctcatg agctgggagc atgaggacgc ggtgcacgcg gtggtgctct ccggagccgg cgaacgcgga ctttgcgccg gcggggacgt ggtggccgtc taccacagtg cccgcaagga cggggtcgag gcgcggcggt tctggcgcca cgagtatctg ctcaacgccc tgatcggccg gttcgccaag ccctacgtgg cgttgatgga cggcatcgta atgggcggcg gcgtcggcgt cagcgcacac gcgaacaccc gggtggttac cgatacctcc aaggtcgcga tgcccgaagt gggcatcggg ttcatccccg acgtcggcgg ggtgtatttg ctgtcgcgtg cacccggcgc gctgggtctg cacgccgccc tgactggagc gccgttttcc ggcgccgacg ccatcgcgct gggattcgcc gaccacttcg tgccacacgg cgacctcgat gcgttcacgc agaagatcgt caccggcggc gtggagagcg cattggccgc ccacgccgtc gaacctccac cgagcacgct tgccgcccaa cgtgattgga tcgacgaatg ctatgccggc gacagcgtcg ccgacatcgt tgcggcgctg cggaaacagg gcggcgaacc agccgtaaat gcttccgacc tgattgccag ccgctccccc atcgcgctgt cggtgacgtt gcaggcagtg cgtcgcgccg ccaaactcga cacgctagaa gacgtgttga tccaggacta tcgggtgtca tcggcgtcgc tgcgctcgca tgacctggtg gagggcatcc gcgcgcagtt gatcgacaag gatcgcaacc cgaactggtc gccggcaacc ctggacgcga tcacggcggc cgacatcgaa gcctatttcg aaccggtcga cgatgacttg agtttcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.00340.a.A1.PW25878
PURIFICATION DATe: 3/31/2009
CONCENTRATION: 29.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTGESHEV LTNVEGGVGF VTLNRPKAIN SLNQTMVDLL ATVLMSWEHE DAVHAVVLSG AGERGLCAGG DVVAVYHSAR KDGVEARRFW RHEYLLNALI GRFAKPYVAL MDGIVMGGGV GVSAHANTRV VTDTSKVAMP EVGIGFIPDV GGVYLLSRAP GALGLHAALT GAPFSGADAI ALGFADHFVP HGDLDAFTQK IVTGGVESAL AAHAVEPPPS TLAAQRDWID ECYAGDSVAD IVAALRKQGG EPAVNASDLI ASRSPIALSV TLQAVRRAAK LDTLEDVLIQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPN WSPATLDAIT AADIEAYFEP VDDDLSF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ccggcgaatc gcatgaggtc ctgacaaacg tcgagggcgg tgtcggattc gtgacgctca accgccccaa ggcgatcaac tcactgaacc aaaccatggt cgacctgctg gccacagtgc tcatgagctg ggagcatgag gacgcggtgc acgcggtggt gctctccgga gccggcgaac gcggactttg cgccggcggg gacgtggtgg ccgtctacca cagtgcccgc aaggacgggg tcgaggcgcg gcggttctgg cgccacgagt atctgctcaa cgccctgatc ggccggttcg ccaagcccta cgtggcgttg atggacggca tcgtaatggg cggcggcgtc ggcgtcagcg cacacgcgaa cacccgggtg gttaccgata cctccaaggt cgcgatgccc gaagtgggca tcgggttcat ccccgacgtc ggcggggtgt atttgctgtc gcgtgcaccc ggcgcgctgg gtctgcacgc cgccctgact ggagcgccgt tttccggcgc cgacgccatc gcgctgggat tcgccgacca cttcgtgcca cacggcgacc tcgatgcgtt cacgcagaag atcgtcaccg gcggcgtgga gagcgcattg gccgcccacg ccgtcgaacc tccaccgagc acgcttgccg cccaacgtga ttggatcgac gaatgctatg ccggcgacag cgtcgccgac atcgttgcgg cgctgcggaa acagggcggc gaaccagccg taaatgcttc cgacctgatt gccagccgct cccccatcgc gctgtcggtg acgttgcagg cagtgcgtcg cgccgccaaa ctcgacacgc tagaagacgt gttgatccag gactatcggg tgtcatcggc gtcgctgcgc tcgcatgacc tggtggaggg catccgcgcg cagttgatcg acaaggatcg caacccgaac tggtcgccgg caaccctgga cgcgatcacg gcggccgaca tcgaagccta tttcgaaccg gtcgacgatg acttgagttt ctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTGESHEV LTNVEGGVGF VTLNRPKAIN SLNQTMVDLL ATVLMSWEHE DAVHAVVLSG AGERGLCAGG DVVAVYHSAR KDGVEARRFW RHEYLLNALI GRFAKPYVAL MDGIVMGGGV GVSAHANTRV VTDTSKVAMP EVGIGFIPDV GGVYLLSRAP GALGLHAALT GAPFSGADAI ALGFADHFVP HGDLDAFTQK IVTGGVESAL AAHAVEPPPS TLAAQRDWID ECYAGDSVAD IVAALRKQGG EPAVNASDLI ASRSPIALSV TLQAVRRAAK LDTLEDVLIQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPN WSPATLDAIT AADIEAYFEP VDDDLSF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGGC GAATCGCATG AGGTCCTGAC AAACGTCGAG GGCGGTGTCG GATTCGTGAC GCTCAACCGC CCCAAGGCGA TCAACTCACT GAACCAAACC ATGGTCGACC TGCTGGCCAC AGTGCTCATG AGCTGGGAGC ATGAGGACGC GGTGCACGCG GTGGTGCTCT CCGGAGCCGG CGAACGCGGA CTTTGCGCCG GCGGGGACGT GGTGGCCGTC TACCACAGTG CCCGCAAGGA CGGGGTCGAG GCGCGGCGGT TCTGGCGCCA CGAGTATCTG CTCAACGCCC TGATCGGCCG GTTCGCCAAG CCCTACGTGG CGTTGATGGA CGGCATCGTA ATGGGCGGCG GCGTCGGCGT CAGCGCACAC GCGAACACCC GGGTGGTTAC CGATACCTCC AAGGTCGCGA TGCCCGAAGT GGGCATCGGG TTCATCCCCG ACGTCGGCGG GGTGTATTTG CTGTCGCGTG CACCCGGCGC GCTGGGTCTG CACGCCGCCC TGACTGGAGC GCCGTTTTCC GGCGCCGACG CCATCGCGCT GGGATTCGCC GACCACTTCG TGCCACACGG CGACCTCGAT GCGTTCACGC AGAAGATCGT CACCGGCGGC GTGGAGAGCG CATTGGCCGC CCACGCCGTC GAACCTCCAC CGAGCACGCT TGCCGCCCAA CGTGATTGGA TCGACGAATG CTATGCCGGC GACAGCGTCG CCGACATCGT TGCGGCGCTG CGGAAACAGG GCGGCGAACC AGCCGTAAAT GCTTCCGACC TGATTGCCAG CCGCTCCCCC ATCGCGCTGT CGGTGACGTT GCAGGCAGTG CGTCGCGCCG CCAAACTCGA CACGCTAGAA GACGTGTTGA TCCAGGACTA TCGGGTGTCA TCGGCGTCGC TGCGCTCGCA TGACCTGGTG GAGGGCATCC GCGCGCAGTT GATCGACAAG GATCGCAACC CGAACTGGTC GCCGGCAACC CTGGACGCGA TCACGGCGGC CGACATCGAA GCCTATTTCG AACCGGTCGA CGATGACTTG AGTTTCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA