SSGCID: Target Page

MysmA.00340.a

Enoyl-CoA hydratase

CENTER ID:	MysmA.00340.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00340.a.A1.GE28611	Full length( MysmA.00340.a )		1	348

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00340.a.A1.PS00540	Full length( MysmA.00340.a )		1	348

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5147
RefSeq:	YP_889522.1
UniProt:	A0R2Y4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTENEDILVN VRNGVGILTL NRPKAINSLT EAMVAEIGKA LHAWENDDSV HTVLLTGAGE RGLCAGGDVV ALYHSAKAGG ADARRFWYDE YLVNAYIGNY PKPYVAVMDG IVMGGGVGVA GHGSIRVVTD TTKMAMPEVG IGFVPDVGGT YLLSRAPGAL GLHAALTGAP FSGADAIAMG FADHYVPHDK LADFIESVVA EGVEQALSAH AVEPPASALM EQRAWIDESY AGATVADILA ALQNHDAPEA RAAADLIATR SPVALAVTLE AVRRAEKLDT LEEALRQEYR ISSAAVRSHD FVEGIRAQLV DKDRNPKWSP ASIADVTQAD IDAYFVPADP ELEFEKEN

NT Sequence

GTGACAGAAA ACGAGGACAT CCTAGTAAAT GTCCGCAACG GTGTCGGCAT CCTCACGCTG AACCGCCCCA AGGCGATCAA CTCGCTGACC GAAGCCATGG TCGCGGAGAT CGGCAAGGCA CTCCACGCAT GGGAGAACGA CGACTCCGTC CACACCGTGC TGCTCACCGG TGCCGGTGAG CGGGGTCTGT GTGCCGGCGG CGACGTCGTG GCGCTCTATC ACAGCGCGAA GGCCGGCGGC GCGGACGCGC GCCGATTCTG GTACGACGAG TACCTGGTCA ACGCCTACAT CGGCAATTAC CCCAAACCGT ACGTCGCCGT GATGGACGGC ATCGTGATGG GCGGTGGCGT CGGCGTGGCC GGCCACGGCA GCATCCGGGT GGTCACCGAC ACCACCAAGA TGGCGATGCC CGAGGTCGGC ATCGGCTTCG TTCCCGACGT GGGCGGCACC TATCTGCTCT CGCGCGCCCC CGGCGCGCTC GGTCTGCACG CCGCGCTCAC GGGTGCGCCG TTCTCGGGGG CCGACGCGAT CGCCATGGGC TTCGCCGATC ACTATGTGCC GCACGACAAG CTCGCCGATT TCATCGAGTC CGTCGTTGCC GAAGGTGTCG AGCAGGCCCT GTCCGCGCAC GCCGTTGAAC CGCCGGCCAG CGCCCTGATG GAACAGCGGG CCTGGATCGA CGAGTCCTAC GCGGGCGCGA CGGTCGCCGA CATCCTCGCG GCGCTGCAGA ACCACGACGC CCCGGAGGCC AGGGCCGCCG CCGATCTGAT CGCCACGCGC TCCCCCGTCG CGCTGGCGGT GACGCTTGAG GCGGTGCGCC GGGCCGAGAA ACTGGACACC CTCGAAGAGG CGCTGCGCCA GGAGTACCGG ATCTCCAGCG CCGCGGTGAG ATCGCATGAT TTCGTCGAGG GCATCCGGGC CCAGTTGGTG GACAAGGACC GCAACCCGAA GTGGTCACCC GCCTCGATCG CCGATGTCAC CCAGGCCGAC ATCGACGCCT ATTTCGTTCC GGCGGACCCG GAACTGGAGT TCGAGAAGGA GAAC

Details for MysmA.00340.a.A1.GE28611

HARVESTED ON:	2/17/2010
SEQUENCED ON:	2/24/2010
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Validated AA Sequence

MVTENEDILV NVRNGVGILT LNRPKAINSL TEAMVAEIGK ALHAWENDDS VHTVLLTGAG ERGLCAGGDV VALYHSAKAG GADARRFWYD EYLVNAYIGN YPKPYVAVMD GIVMGGGVGV AGHGSIRVVT DTTKMAMPEV GIGFVPDVGG TYLLSRAPGA LGLHAALTGA PFSGADAIAM GFADHYVPHD KLADFIESVV AEGVEQALSA HAVEPPASAL MEQRAWIDES YAGATVADIL AALQNHDAPE ARAAADLIAT RSPVALAVTL EAVRRAEKLD TLEEALRQEY RISSAAVRSH DFVEGIRAQL VDKDRNPKWS PASIADVTQA DIDAYFVPA

Validated NT Sequence

ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tgacagaaaa cgaggacatc ctagtaaatg tccgcaacgg tgtcggcatc ctcacgctga accgccccaa ggcgatcaac tcgctgaccg aagccatggt cgcggagatc ggcaaggcac tccacgcatg ggagaacgac gactccgtcc acaccgtgct gctcaccggt gccggtgagc ggggtctgtg tgccggcggc gacgtcgtgg cgctctatca cagcgcgaag gccggcggcg cggacgcgcg ccgattctgg tacgacgagt acctggtcaa cgcctacatc ggcaattacc ccaaaccgta cgtcgccgtg atggacggca tcgtgatggg cggtggcgtc ggcgtggccg gccacggcag catccgggtg gtcaccgaca ccaccaagat ggcgatgccc gaggtcggca tcggcttcgt tcccgacgtg ggcggcacct atctgctctc gcgcgccccc ggcgcgctcg gtctgcacgc cgcgctcacg ggtgcgccgt tctcgggggc cgacgcgatc gccatgggct tcgccgatca ctatgtgccg cacgacaagc tcgccgattt catcgagtcc gtcgttgccg aaggtgtcga gcaggccctg tccgcgcacg ccgttgaacc gccggccagc gccctgatgg aacagcgggc ctggatcgac gagtcctacg cgggcgcgac ggtcgccgac atcctcgcgg cgctgcagaa ccacgacgcc ccggaggcca gggccgccgc cgatctgatc gccacgcgct cccccgtcgc gctggcggtg acgcttgagg cggtgcgccg ggccgagaaa ctggacaccc tcgaagaggc gctgcgccag gagtaccgga tctccagcgc cgcggtgaga tcgcatgatt tcgtcgaggg catccgggcc cagttggtgg acaaggaccg caacccgaag tggtcacccg cctcgatcgc cgatgtcacc caggccgaca tcgacgccta tttcgttccg gcgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTENEDIL VNVRNGVGIL TLNRPKAINS LTEAMVAEIG KALHAWENDD SVHTVLLTGA GERGLCAGGD VVALYHSAKA GGADARRFWY DEYLVNAYIG NYPKPYVAVM DGIVMGGGVG VAGHGSIRVV TDTTKMAMPE VGIGFVPDVG GTYLLSRAPG ALGLHAALTG APFSGADAIA MGFADHYVPH DKLADFIESV VAEGVEQALS AHAVEPPASA LMEQRAWIDE SYAGATVADI LAALQNHDAP EARAAADLIA TRSPVALAVT LEAVRRAEKL DTLEEALRQE YRISSAAVRS HDFVEGIRAQ LVDKDRNPKW SPASIADVTQ ADIDAYFVPA DPELEFEKEN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacagaa aacgaggaca tcctagtaaa tgtccgcaac ggtgtcggca tcctcacgct gaaccgcccc aaggcgatca actcgctgac cgaagccatg gtcgcggaga tcggcaaggc actccacgca tgggagaacg acgactccgt ccacaccgtg ctgctcaccg gtgccggtga gcggggtctg tgtgccggcg gcgacgtcgt ggcgctctat cacagcgcga aggccggcgg cgcggacgcg cgccgattct ggtacgacga gtacctggtc aacgcctaca tcggcaatta ccccaaaccg tacgtcgccg tgatggacgg catcgtgatg ggcggtggcg tcggcgtggc cggccacggc agcatccggg tggtcaccga caccaccaag atggcgatgc ccgaggtcgg catcggcttc gttcccgacg tgggcggcac ctatctgctc tcgcgcgccc ccggcgcgct cggtctgcac gccgcgctca cgggtgcgcc gttctcgggg gccgacgcga tcgccatggg cttcgccgat cactatgtgc cgcacgacaa gctcgccgat ttcatcgagt ccgtcgttgc cgaaggtgtc gagcaggccc tgtccgcgca cgccgttgaa ccgccggcca gcgccctgat ggaacagcgg gcctggatcg acgagtccta cgcgggcgcg acggtcgccg acatcctcgc ggcgctgcag aaccacgacg ccccggaggc cagggccgcc gccgatctga tcgccacgcg ctcccccgtc gcgctggcgg tgacgcttga ggcggtgcgc cgggccgaga aactggacac cctcgaagag gcgctgcgcc aggagtaccg gatctccagc gccgcggtga gatcgcatga tttcgtcgag ggcatccggg cccagttggt ggacaaggac cgcaacccga agtggtcacc cgcctcgatc gccgatgtca cccaggccga catcgacgcc tatttcgttc cggcggaccc ggaactggag ttcgagaagg agaacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MysmA.00340.a.A1.PS00540

PURIFICATION DATe:	3/31/2010
CONCENTRATION:	23.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACAGAA AACGAGGACA TCCTAGTAAA TGTCCGCAAC GGTGTCGGCA TCCTCACGCT GAACCGCCCC AAGGCGATCA ACTCGCTGAC CGAAGCCATG GTCGCGGAGA TCGGCAAGGC ACTCCACGCA TGGGAGAACG ACGACTCCGT CCACACCGTG CTGCTCACCG GTGCCGGTGA GCGGGGTCTG TGTGCCGGCG GCGACGTCGT GGCGCTCTAT CACAGCGCGA AGGCCGGCGG CGCGGACGCG CGCCGATTCT GGTACGACGA GTACCTGGTC AACGCCTACA TCGGCAATTA CCCCAAACCG TACGTCGCCG TGATGGACGG CATCGTGATG GGCGGTGGCG TCGGCGTGGC CGGCCACGGC AGCATCCGGG TGGTCACCGA CACCACCAAG ATGGCGATGC CCGAGGTCGG CATCGGCTTC GTTCCCGACG TGGGCGGCAC CTATCTGCTC TCGCGCGCCC CCGGCGCGCT CGGTCTGCAC GCCGCGCTCA CGGGTGCGCC GTTCTCGGGG GCCGACGCGA TCGCCATGGG CTTCGCCGAT CACTATGTGC CGCACGACAA GCTCGCCGAT TTCATCGAGT CCGTCGTTGC CGAAGGTGTC GAGCAGGCCC TGTCCGCGCA CGCCGTTGAA CCGCCGGCCA GCGCCCTGAT GGAACAGCGG GCCTGGATCG ACGAGTCCTA CGCGGGCGCG ACGGTCGCCG ACATCCTCGC GGCGCTGCAG AACCACGACG CCCCGGAGGC CAGGGCCGCC GCCGATCTGA TCGCCACGCG CTCCCCCGTC GCGCTGGCGG TGACGCTTGA GGCGGTGCGC CGGGCCGAGA AACTGGACAC CCTCGAAGAG GCGCTGCGCC AGGAGTACCG GATCTCCAGC GCCGCGGTGA GATCGCATGA TTTCGTCGAG GGCATCCGGG CCCAGTTGGT GGACAAGGAC CGCAACCCGA AGTGGTCACC CGCCTCGATC GCCGATGTCA CCCAGGCCGA CATCGACGCC TATTTCGTTC CGGCGGACCC GGAACTGGAG TTCGAGAAGG AGAACAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA