MyulA.00340.a

Enoyl-CoA hydratase EchA9

CENTER ID: MyulA.00340.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00340.a.A1.GE29680 Full length( MyulA.00340.a ) 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00340.a.A1.PW29580 Full length( MyulA.00340.a ) 1 345

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.324
RefSeq: YP_904430.1
UniProt: A0PKU0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGESDEVLT RVDGGVGFIT LNRPKAINSL NQTMVDLLST VFTRWERDGE VRAVVVSGAG ERGLCAGGDV VAVYHSARKD GAEARRFWRD EYLMNGQIGR FSKPYVALMD GIVMGGGVGV SAHGDIRVVT DTSKVAMPEV GIGFIPDVGG AYLLSRAPGA LGLHTALTGA PFSGADAIAL GFADHFVPHA ELGAFSAAIA SDGIESALAH HAVEPPPSGL AAQRGWIDEC YAGDTVADII AALRGHDAGP ANDAADVIAS RSPIALSVTL EAVRRAAKLE TLEDVLVQDY RVSSASLRSH DLVEGIRAQL IDKDRNPKWS PATFAEITES DVAAYFAPVD DDLSF
NT Sequence
GTGACCGGCG AGTCCGACGA AGTTCTGACC CGCGTCGACG GCGGTGTCGG CTTTATTACC CTCAACCGCC CCAAAGCGAT CAACTCGCTG AACCAGACAA TGGTGGACCT GCTGAGCACG GTGTTCACCC GCTGGGAGCG CGACGGCGAG GTACGCGCGG TGGTGGTCTC CGGAGCCGGC GAACGCGGAT TGTGTGCCGG CGGTGATGTG GTGGCCGTCT ATCACAGTGC CCGCAAGGAC GGGGCCGAAG CGCGGCGGTT CTGGCGCGAC GAGTATCTGA TGAACGGCCA GATCGGCCGG TTCAGCAAGC CGTATGTGGC CTTGATGGAC GGCATCGTGA TGGGCGGCGG CGTGGGCGTG AGCGCCCACG GCGACATCCG GGTGGTGACC GATACCTCCA AGGTCGCGAT GCCCGAGGTC GGTATCGGGT TCATTCCCGA CGTCGGCGGG GCGTACCTGC TGTCGCGGGC CCCCGGCGCC CTGGGATTGC ACACGGCACT CACCGGAGCA CCCTTCTCCG GTGCCGACGC CATCGCACTG GGGTTCGCCG ACCACTTCGT GCCGCACGCC GAACTCGGCG CCTTCAGCGC GGCCATCGCC TCCGACGGAA TCGAGAGCGC GCTGGCCCAC CACGCCGTCG AACCGCCACC CAGCGGTCTC GCCGCCCAAC GCGGCTGGAT CGACGAGTGC TATGCGGGTG ATACGGTCGC CGACATCATC GCCGCACTGC GCGGCCACGA TGCCGGCCCG GCCAACGACG CCGCCGATGT GATTGCCAGC CGCTCCCCCA TCGCACTGTC GGTGACGTTG GAGGCGGTGC GTCGTGCAGC CAAGCTGGAA ACGCTCGAAG ACGTCTTGGT CCAGGACTAT CGGGTGTCCT CGGCGTCGTT GCGCTCGCAC GACCTGGTGG AGGGCATCCG CGCACAGTTG ATCGATAAGG ACCGCAATCC GAAATGGTCG CCGGCGACGT TCGCCGAGAT CACCGAGTCC GACGTCGCAG CGTATTTCGC GCCGGTCGAC GACGACTTGA GTTTC
Details for MyulA.00340.a.A1.GE29680
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 93
PERCENT COVERAGE: 94
Validated AA Sequence
MVTGESDEVL TRXXXGXXFI TLNRPKAINS LNQTMVDXXX XXXXXWXXXX XVRAXVXXGA GERGLCXGGD VVAVYHSARK DGAEARRFWR DEYLMNXQIG RFSKPYVALM DGIVMGGGVG VSAHGDIRVV TDTSKVAMPE VGIGFIPDVG GAYLLSRAPG ALGLHTALTG APFSGADAIA LGFADHFVPH AELGAFSAAI ASDGIESALA HHAVEPPPSG LAAQRGWIDE CYAGDTVADI IAALRGHDAG PANDAADVIA SRSPIALSVT LEAVRRAAKL ETLEDVLVQD YRVSSASLRS HDLVEGIRAQ LIDKDRNPKW SPATFAEITE SDVAAYFAPV DDDLSF
Validated NT Sequence
tagaaactca agtcgtcgtc gaccggcgcg aaatacgctg cgacgtcgga ctcggtgatc tcggcgaacg tcgccggcga ccatttcgga ttgcggtcct tatcgatcaa ctgtgcgcgg atgccctcca ccaggtcgtg cgagcgcaac gacgccgagg acacccgata gtcctggacc aagacgtctt cgagcgtttc cagcttggct gcacgacgca ccgcctccaa cgtcaccgac agtgcgatgg gggagcggct ggcaatcaca tcggcggcgt cgttggccgg gccggcatcg tggccgcgca gtgcggcgat gatgtcggcg accgtatcac ccgcatagca ctcgtcgatc cagccgcgtt gggcggcgag accgctgggt ggcggttcga cggcgtggtg ggccagcgcg ctctcgattc cgtcggaggc gatggccgcg ctgaaggcgc cgagttcggc gtgcggcacg aagtggtcgg cgaaccccag tgcgatggcg tcggcaccgg agaagggtgc tccggtgagt gccgtgtgca atcccagggc gccgggggcc cgcgacagca ggtacgcccc gccgacgtcg ggaatgaacc cgataccgac ctcgggcatc gcgaccttgg aggtatcggt caccacccgg atgtcgccgt gggcgctcac gcccacgccg ccgcccatca cgatgccgtc catcaaggcc acatacggct tgctgaaccg gccgatctgg ncgttcatca gatactcgtc gcgccagaac cgccgcgctt cngccccgtc cttgcgggca ctgtgataga cggccaccac atcaccgccg ncacacaatc cgcgttcgcc ggctccnnng nccaccnccg cgcgtaccnt gcngnnnngc ncccagnnnn nnnnnnnnnn nnnnnnnnng tccaccattg tctggttcag cgagttgatc gctttggggc ggttgagggt aataaannnn nnaccncnnt ngnngcgggt cagaacttcg tcggactcgc cggtcaccat cgaaccagga ccctgggtct gagcttccag ggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTGESDEV LTRVDGGVGF ITLNRPKAIN SLNQTMVDLL STVFTRWERD GEVRAVVVSG AGERGLCAGG DVVAVYHSAR KDGAEARRFW RDEYLMNGQI GRFSKPYVAL MDGIVMGGGV GVSAHGDIRV VTDTSKVAMP EVGIGFIPDV GGAYLLSRAP GALGLHTALT GAPFSGADAI ALGFADHFVP HAELGAFSAA IASDGIESAL AHHAVEPPPS GLAAQRGWID ECYAGDTVAD IIAALRGHDA GPANDAADVI ASRSPIALSV TLEAVRRAAK LETLEDVLVQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPK WSPATFAEIT ESDVAAYFAP VDDDLSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccggc gagtccgacg aagttctgac ccgcgtcgac ggcggtgtcg gctttattac cctcaaccgc cccaaagcga tcaactcgct gaaccagaca atggtggacc tgctgagcac ggtgttcacc cgctgggagc gcgacggcga ggtacgcgcg gtggtggtct ccggagccgg cgaacgcgga ttgtgtgccg gcggtgatgt ggtggccgtc tatcacagtg cccgcaagga cggggccgaa gcgcggcggt tctggcgcga cgagtatctg atgaacggcc agatcggccg gttcagcaag ccgtatgtgg ccttgatgga cggcatcgtg atgggcggcg gcgtgggcgt gagcgcccac ggcgacatcc gggtggtgac cgatacctcc aaggtcgcga tgcccgaggt cggtatcggg ttcattcccg acgtcggcgg ggcgtacctg ctgtcgcggg cccccggcgc cctgggattg cacacggcac tcaccggagc acccttctcc ggtgccgacg ccatcgcact ggggttcgcc gaccacttcg tgccgcacgc cgaactcggc gccttcagcg cggccatcgc ctccgacgga atcgagagcg cgctggccca ccacgccgtc gaaccgccac ccagcggtct cgccgcccaa cgcggctgga tcgacgagtg ctatgcgggt gatacggtcg ccgacatcat cgccgcactg cgcggccacg atgccggccc ggccaacgac gccgccgatg tgattgccag ccgctccccc atcgcactgt cggtgacgtt ggaggcggtg cgtcgtgcag ccaagctgga aacgctcgaa gacgtcttgg tccaggacta tcgggtgtcc tcggcgtcgt tgcgctcgca cgacctggtg gagggcatcc gcgcacagtt gatcgataag gaccgcaatc cgaaatggtc gccggcgacg ttcgccgaga tcaccgagtc cgacgtcgca gcgtatttcg cgccggtcga cgacgacttg agtttcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyulA.00340.a.A1.PW29580
PURIFICATION DATe: 1/10/2011
CONCENTRATION: 24.63mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 93
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MVTGESDEVL TRXXXGXXFI TLNRPKAINS LNQTMVDXXX XXXXXWXXXX XVRAXVXXGA GERGLCXGGD VVAVYHSARK DGAEARRFWR DEYLMNXQIG RFSKPYVALM DGIVMGGGVG VSAHGDIRVV TDTSKVAMPE VGIGFIPDVG GAYLLSRAPG ALGLHTALTG APFSGADAIA LGFADHFVPH AELGAFSAAI ASDGIESALA HHAVEPPPSG LAAQRGWIDE CYAGDTVADI IAALRGHDAG PANDAADVIA SRSPIALSVT LEAVRRAAKL ETLEDVLVQD YRVSSASLRS HDLVEGIRAQ LIDKDRNPKW SPATFAEITE SDVAAYFAPV DDDLSF
Validated NT Sequence
tagaaactca agtcgtcgtc gaccggcgcg aaatacgctg cgacgtcgga ctcggtgatc tcggcgaacg tcgccggcga ccatttcgga ttgcggtcct tatcgatcaa ctgtgcgcgg atgccctcca ccaggtcgtg cgagcgcaac gacgccgagg acacccgata gtcctggacc aagacgtctt cgagcgtttc cagcttggct gcacgacgca ccgcctccaa cgtcaccgac agtgcgatgg gggagcggct ggcaatcaca tcggcggcgt cgttggccgg gccggcatcg tggccgcgca gtgcggcgat gatgtcggcg accgtatcac ccgcatagca ctcgtcgatc cagccgcgtt gggcggcgag accgctgggt ggcggttcga cggcgtggtg ggccagcgcg ctctcgattc cgtcggaggc gatggccgcg ctgaaggcgc cgagttcggc gtgcggcacg aagtggtcgg cgaaccccag tgcgatggcg tcggcaccgg agaagggtgc tccggtgagt gccgtgtgca atcccagggc gccgggggcc cgcgacagca ggtacgcccc gccgacgtcg ggaatgaacc cgataccgac ctcgggcatc gcgaccttgg aggtatcggt caccacccgg atgtcgccgt gggcgctcac gcccacgccg ccgcccatca cgatgccgtc catcaaggcc acatacggct tgctgaaccg gccgatctgg ncgttcatca gatactcgtc gcgccagaac cgccgcgctt cngccccgtc cttgcgggca ctgtgataga cggccaccac atcaccgccg ncacacaatc cgcgttcgcc ggctccnnng nccaccnccg cgcgtaccnt gcngnnnngc ncccagnnnn nnnnnnnnnn nnnnnnnnng tccaccattg tctggttcag cgagttgatc gctttggggc ggttgagggt aataaannnn nnaccncnnt ngnngcgggt cagaacttcg tcggactcgc cggtcaccat cgaaccagga ccctgggtct gagcttccag ggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTGESDEV LTRVDGGVGF ITLNRPKAIN SLNQTMVDLL STVFTRWERD GEVRAVVVSG AGERGLCAGG DVVAVYHSAR KDGAEARRFW RDEYLMNGQI GRFSKPYVAL MDGIVMGGGV GVSAHGDIRV VTDTSKVAMP EVGIGFIPDV GGAYLLSRAP GALGLHTALT GAPFSGADAI ALGFADHFVP HAELGAFSAA IASDGIESAL AHHAVEPPPS GLAAQRGWID ECYAGDTVAD IIAALRGHDA GPANDAADVI ASRSPIALSV TLEAVRRAAK LETLEDVLVQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPK WSPATFAEIT ESDVAAYFAP VDDDLSF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGGC GAGTCCGACG AAGTTCTGAC CCGCGTCGAC GGCGGTGTCG GCTTTATTAC CCTCAACCGC CCCAAAGCGA TCAACTCGCT GAACCAGACA ATGGTGGACC TGCTGAGCAC GGTGTTCACC CGCTGGGAGC GCGACGGCGA GGTACGCGCG GTGGTGGTCT CCGGAGCCGG CGAACGCGGA TTGTGTGCCG GCGGTGATGT GGTGGCCGTC TATCACAGTG CCCGCAAGGA CGGGGCCGAA GCGCGGCGGT TCTGGCGCGA CGAGTATCTG ATGAACGGCC AGATCGGCCG GTTCAGCAAG CCGTATGTGG CCTTGATGGA CGGCATCGTG ATGGGCGGCG GCGTGGGCGT GAGCGCCCAC GGCGACATCC GGGTGGTGAC CGATACCTCC AAGGTCGCGA TGCCCGAGGT CGGTATCGGG TTCATTCCCG ACGTCGGCGG GGCGTACCTG CTGTCGCGGG CCCCCGGCGC CCTGGGATTG CACACGGCAC TCACCGGAGC ACCCTTCTCC GGTGCCGACG CCATCGCACT GGGGTTCGCC GACCACTTCG TGCCGCACGC CGAACTCGGC GCCTTCAGCG CGGCCATCGC CTCCGACGGA ATCGAGAGCG CGCTGGCCCA CCACGCCGTC GAACCGCCAC CCAGCGGTCT CGCCGCCCAA CGCGGCTGGA TCGACGAGTG CTATGCGGGT GATACGGTCG CCGACATCAT CGCCGCACTG CGCGGCCACG ATGCCGGCCC GGCCAACGAC GCCGCCGATG TGATTGCCAG CCGCTCCCCC ATCGCACTGT CGGTGACGTT GGAGGCGGTG CGTCGTGCAG CCAAGCTGGA AACGCTCGAA GACGTCTTGG TCCAGGACTA TCGGGTGTCC TCGGCGTCGT TGCGCTCGCA CGACCTGGTG GAGGGCATCC GCGCACAGTT GATCGATAAG GACCGCAATC CGAAATGGTC GCCGGCGACG TTCGCCGAGA TCACCGAGTC CGACGTCGCA GCGTATTTCG CGCCGGTCGA CGACGACTTG AGTTTCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA