MyleA.00340.a

Putative enoyl-CoA hydratase/isomerase

CENTER ID: MyleA.00340.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00340.a.A1.GE29207 Full length( MyleA.00340.a ) 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00340.a.A1.PS00709 Full length( MyleA.00340.a ) 1 345

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4641
RefSeq: YP_002504184.1
UniProt: A0A0H3MRZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGESDEVLA RVEAGIGLIT LNRPKAINSL NQQMVDALTA ILTSWADDDA VRAVVLSGAG ERGLCAGGDV VAIYHSALKD GVEARRFWRS EYLLNSQIAR FPKPYVSLMD GIVMGGGVGI GAHANTRVVT DTSQVAMPEV GIGFIPDVGG TYLLSRAPGA LGLHVALTGA PFSGADAIEL GFADHYVPHS DLNAFTRAVV TDGVNSALAG YAIEPPPSYL AAQRDWIDEC YVGETVKDII TALRGHDAEP AEGAFNLIVS RSPVALSVTL EAMRRAAKLE TLTDVLTQDY RVSSASLHSH DLVEGIRAQL VHKDRNPQWA PTTLAEVTAA DICGYFEPVD DDLSF
NT Sequence
GTGGCTGGCG AATCAGATGA GGTCTTGGCG CGCGTTGAGG CCGGTATCGG CCTTATCACA CTTAACCGCC CTAAGGCGAT CAACTCGCTG AACCAGCAGA TGGTGGACGC GCTGACCGCC ATACTCACCA GCTGGGCAGA CGACGACGCG GTACGTGCAG TGGTGCTATC TGGAGCCGGG GAACGCGGAC TATGCGCCGG TGGCGATGTG GTGGCGATCT ATCACAGTGC TCTCAAGGAT GGGGTTGAGG CACGGCGGTT TTGGCGCAGC GAATATCTGC TCAACAGCCA AATCGCCCGG TTCCCCAAGC CGTACGTGTC GTTGATGGAC GGCATCGTGA TGGGCGGTGG CGTCGGCATC GGTGCACACG CAAACACTCG AGTGGTAACC GATACCTCGC AAGTGGCAAT GCCCGAGGTC GGCATCGGGT TTATCCCAGA CGTCGGCGGG ACATATCTGT TGTCTCGGGC GCCCGGTGCG CTGGGTCTGC ATGTTGCGCT AACCGGAGCA CCGTTTTCCG GTGCCGACGC TATCGAGCTG GGCTTCGCTG ACCACTATGT GCCGCACAGC GACCTTAACG CGTTCACCCG AGCGGTCGTT ACCGACGGCG TTAACAGTGC GCTAGCCGGC TACGCTATCG AACCTCCACC AAGCTACCTT GCCGCACAAC GTGATTGGAT CGACGAATGC TACGTTGGCG AAACCGTCAA AGACATCATC ACCGCTTTGC GTGGGCATGA TGCGGAACCT GCTGAAGGCG CCTTCAACCT GATCGTTAGC CGCTCCCCCG TCGCATTGTC AGTCACACTG GAGGCGATGC GTCGAGCAGC CAAACTGGAA ACACTGACAG ATGTTCTGAC TCAAGACTAT CGGGTGTCGT CGGCTTCACT GCACTCACAC GACCTGGTGG AAGGCATCCG CGCGCAGCTG GTCCACAAGG ATCGCAATCC ACAATGGGCG CCGACGACAC TGGCCGAAGT CACTGCAGCC GACATCTGTG GATATTTCGA GCCGGTTGAC GATGACTTGA GTTTC
Details for MyleA.00340.a.A1.GE29207
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVAGESX EVLARVEAGI GLITLNRPKA INSLNQQMVD ALTAILTSWA DDDAVRAVVL SGAGERGLCA GGDVVAIYHS ALKDGVEARR FWRSEYLLNS QIARFPKPYV SLMDGIVMGG GVGIGAHANT RVVTDTSQVA MPEVGIGFIP DVGGTYLLSR APGALGLHVA LTGAPFSGAD AIELGFADHY VPHSDLNAFT RAVVTDGVNS ALAGYAIEPP PSYLAAQRDW IDECYVGETV KDIITALRGH DAEPAEGAFN LIVSRSPVAL SVTLEAMRRA AKLETLTDVL TQDYRVSSAS LHSHDLVEGI RAQLVHKDRN PQWAPTTLAE VTAADICGYF EPVDDDLSF
Validated NT Sequence
ttagaaactc aagtcatcgt caaccggctc gaaatatcca cagatgtcgg ctgcagtgac ttcggccagt gtcgtcggcg cccattgtgg attgcgatcc ttgtggacca gctgcgcgcg gatgccttcc accaggtcgt gtgagtgcag tgaagccgac gacacccgat agtcttgagt cagaacatct gtcagtgttt ccagtttggc tgctcgacgc atcgcctcca gtgtgactga caatgcgacg ggggagcggc taacgatcag gttgaaggcg ccttcagcag gttccgcatc atgcccacgc aaagcggtga tgatgtcttt gacggtttcg ccaacgtagc attcgtcgat ccaatcacgt tgtgcggcaa ggtagcttgg tggaggttcg atagcgtagc cggctagcgc actgttaacg ccgtcggtaa cgaccgctcg ggtgaacgcg ttaaggtcgc tgtgcggcac atagtggtca gcgaagccca gctcgatagc gtcggcaccg gaaaacggtg ctccggttag cgcaacatgc agacccagcg caccgggcgc ccgagacaac agatatgtcc cgccgacgtc tgggataaac ccgatgccga cctcgggcat tgccacttgc gaggtatcgg ttaccactcg agtgtttgcg tgtgcaccga tgccgacgcc accgcccatc acgatgccgt ccatcaacga cacgtacggc ttggggaacc gggcgatttg gctgttgagc agatattcgc tgcgccaaaa ccgccgtgcc tcaaccccat ccttgagagc actgtgatag atcgccacca catcgccacc ggcgcatagt ccgcgttccc cggctccaga tagcaccact gcacgtaccg cgtcgtcgtc tgcccagctg gtgagtatgg cggtcagcgc gtccaccatc tgctggttca gcgagttgat cgccttaggg cggttaagtg tgataaggcc gataccggcc tcaacgcgcg ccaagacctc atntgattcg ccagccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGESDEV LARVEAGIGL ITLNRPKAIN SLNQQMVDAL TAILTSWADD DAVRAVVLSG AGERGLCAGG DVVAIYHSAL KDGVEARRFW RSEYLLNSQI ARFPKPYVSL MDGIVMGGGV GIGAHANTRV VTDTSQVAMP EVGIGFIPDV GGTYLLSRAP GALGLHVALT GAPFSGADAI ELGFADHYVP HSDLNAFTRA VVTDGVNSAL AGYAIEPPPS YLAAQRDWID ECYVGETVKD IITALRGHDA EPAEGAFNLI VSRSPVALSV TLEAMRRAAK LETLTDVLTQ DYRVSSASLH SHDLVEGIRA QLVHKDRNPQ WAPTTLAEVT AADICGYFEP VDDDLSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctggc gaatcagatg aggtcttggc gcgcgttgag gccggtatcg gccttatcac acttaaccgc cctaaggcga tcaactcgct gaaccagcag atggtggacg cgctgaccgc catactcacc agctgggcag acgacgacgc ggtacgtgca gtggtgctat ctggagccgg ggaacgcgga ctatgcgccg gtggcgatgt ggtggcgatc tatcacagtg ctctcaagga tggggttgag gcacggcggt tttggcgcag cgaatatctg ctcaacagcc aaatcgcccg gttccccaag ccgtacgtgt cgttgatgga cggcatcgtg atgggcggtg gcgtcggcat cggtgcacac gcaaacactc gagtggtaac cgatacctcg caagtggcaa tgcccgaggt cggcatcggg tttatcccag acgtcggcgg gacatatctg ttgtctcggg cgcccggtgc gctgggtctg catgttgcgc taaccggagc accgttttcc ggtgccgacg ctatcgagct gggcttcgct gaccactatg tgccgcacag cgaccttaac gcgttcaccc gagcggtcgt taccgacggc gttaacagtg cgctagccgg ctacgctatc gaacctccac caagctacct tgccgcacaa cgtgattgga tcgacgaatg ctacgttggc gaaaccgtca aagacatcat caccgctttg cgtgggcatg atgcggaacc tgctgaaggc gccttcaacc tgatcgttag ccgctccccc gtcgcattgt cagtcacact ggaggcgatg cgtcgagcag ccaaactgga aacactgaca gatgttctga ctcaagacta tcgggtgtcg tcggcttcac tgcactcaca cgacctggtg gaaggcatcc gcgcgcagct ggtccacaag gatcgcaatc cacaatgggc gccgacgaca ctggccgaag tcactgcagc cgacatctgt ggatatttcg agccggttga cgatgacttg agtttcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyleA.00340.a.A1.PS00709
PURIFICATION DATe: 8/9/2010
CONCENTRATION: 45.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVAGESX EVLARVEAGI GLITLNRPKA INSLNQQMVD ALTAILTSWA DDDAVRAVVL SGAGERGLCA GGDVVAIYHS ALKDGVEARR FWRSEYLLNS QIARFPKPYV SLMDGIVMGG GVGIGAHANT RVVTDTSQVA MPEVGIGFIP DVGGTYLLSR APGALGLHVA LTGAPFSGAD AIELGFADHY VPHSDLNAFT RAVVTDGVNS ALAGYAIEPP PSYLAAQRDW IDECYVGETV KDIITALRGH DAEPAEGAFN LIVSRSPVAL SVTLEAMRRA AKLETLTDVL TQDYRVSSAS LHSHDLVEGI RAQLVHKDRN PQWAPTTLAE VTAADICGYF EPVDDDLSF
Validated NT Sequence
ttagaaactc aagtcatcgt caaccggctc gaaatatcca cagatgtcgg ctgcagtgac ttcggccagt gtcgtcggcg cccattgtgg attgcgatcc ttgtggacca gctgcgcgcg gatgccttcc accaggtcgt gtgagtgcag tgaagccgac gacacccgat agtcttgagt cagaacatct gtcagtgttt ccagtttggc tgctcgacgc atcgcctcca gtgtgactga caatgcgacg ggggagcggc taacgatcag gttgaaggcg ccttcagcag gttccgcatc atgcccacgc aaagcggtga tgatgtcttt gacggtttcg ccaacgtagc attcgtcgat ccaatcacgt tgtgcggcaa ggtagcttgg tggaggttcg atagcgtagc cggctagcgc actgttaacg ccgtcggtaa cgaccgctcg ggtgaacgcg ttaaggtcgc tgtgcggcac atagtggtca gcgaagccca gctcgatagc gtcggcaccg gaaaacggtg ctccggttag cgcaacatgc agacccagcg caccgggcgc ccgagacaac agatatgtcc cgccgacgtc tgggataaac ccgatgccga cctcgggcat tgccacttgc gaggtatcgg ttaccactcg agtgtttgcg tgtgcaccga tgccgacgcc accgcccatc acgatgccgt ccatcaacga cacgtacggc ttggggaacc gggcgatttg gctgttgagc agatattcgc tgcgccaaaa ccgccgtgcc tcaaccccat ccttgagagc actgtgatag atcgccacca catcgccacc ggcgcatagt ccgcgttccc cggctccaga tagcaccact gcacgtaccg cgtcgtcgtc tgcccagctg gtgagtatgg cggtcagcgc gtccaccatc tgctggttca gcgagttgat cgccttaggg cggttaagtg tgataaggcc gataccggcc tcaacgcgcg ccaagacctc atntgattcg ccagccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGESDEV LARVEAGIGL ITLNRPKAIN SLNQQMVDAL TAILTSWADD DAVRAVVLSG AGERGLCAGG DVVAIYHSAL KDGVEARRFW RSEYLLNSQI ARFPKPYVSL MDGIVMGGGV GIGAHANTRV VTDTSQVAMP EVGIGFIPDV GGTYLLSRAP GALGLHVALT GAPFSGADAI ELGFADHYVP HSDLNAFTRA VVTDGVNSAL AGYAIEPPPS YLAAQRDWID ECYVGETVKD IITALRGHDA EPAEGAFNLI VSRSPVALSV TLEAMRRAAK LETLTDVLTQ DYRVSSASLH SHDLVEGIRA QLVHKDRNPQ WAPTTLAEVT AADICGYFEP VDDDLSF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCTGGC GAATCAGATG AGGTCTTGGC GCGCGTTGAG GCCGGTATCG GCCTTATCAC ACTTAACCGC CCTAAGGCGA TCAACTCGCT GAACCAGCAG ATGGTGGACG CGCTGACCGC CATACTCACC AGCTGGGCAG ACGACGACGC GGTACGTGCA GTGGTGCTAT CTGGAGCCGG GGAACGCGGA CTATGCGCCG GTGGCGATGT GGTGGCGATC TATCACAGTG CTCTCAAGGA TGGGGTTGAG GCACGGCGGT TTTGGCGCAG CGAATATCTG CTCAACAGCC AAATCGCCCG GTTCCCCAAG CCGTACGTGT CGTTGATGGA CGGCATCGTG ATGGGCGGTG GCGTCGGCAT CGGTGCACAC GCAAACACTC GAGTGGTAAC CGATACCTCG CAAGTGGCAA TGCCCGAGGT CGGCATCGGG TTTATCCCAG ACGTCGGCGG GACATATCTG TTGTCTCGGG CGCCCGGTGC GCTGGGTCTG CATGTTGCGC TAACCGGAGC ACCGTTTTCC GGTGCCGACG CTATCGAGCT GGGCTTCGCT GACCACTATG TGCCGCACAG CGACCTTAAC GCGTTCACCC GAGCGGTCGT TACCGACGGC GTTAACAGTG CGCTAGCCGG CTACGCTATC GAACCTCCAC CAAGCTACCT TGCCGCACAA CGTGATTGGA TCGACGAATG CTACGTTGGC GAAACCGTCA AAGACATCAT CACCGCTTTG CGTGGGCATG ATGCGGAACC TGCTGAAGGC GCCTTCAACC TGATCGTTAG CCGCTCCCCC GTCGCATTGT CAGTCACACT GGAGGCGATG CGTCGAGCAG CCAAACTGGA AACACTGACA GATGTTCTGA CTCAAGACTA TCGGGTGTCG TCGGCTTCAC TGCACTCACA CGACCTGGTG GAAGGCATCC GCGCGCAGCT GGTCCACAAG GATCGCAATC CACAATGGGC GCCGACGACA CTGGCCGAAG TCACTGCAGC CGACATCTGT GGATATTTCG AGCCGGTTGA CGATGACTTG AGTTTCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA