SSGCID: Target Page

MyabA.00340.a

Probable enoyl-CoA hydratase

CENTER ID:	MyabA.00340.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00340.a.A1.GE30290	Full length( MyabA.00340.a )		1	343

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00340.a.A1.PS00893	Full length( MyabA.00340.a )		1	343

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1224
RefSeq:	YP_001701929.1
UniProt:	B1MKI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDQIETRVE KGVGLLTLNR PKAINSLTYD MIAAMNAVLA EWEADDAVRV VVLSGAGERG LCAGGDVVAV HDSAKIDGVA ARRFFRDEYV LNAQIARFPK PYVALMDGIV MGGGVGVGGH ANIRIVTDTS KVAMPEVGIG FIPDVGGTYL LSRAPGQLGL HAALTGAPFS GADAIALGFA DHYVPHDRLE KFTKAIIDSD IATALAVHGT NAPSSDLLAQ RNWIDECYGG ESVADIVAAL QARGDERARA AADQILTRSP IALSVTLAAV RRARELPSLE AVLTQEYRVS SQSVRSHDLV EGIRAQLVDK DRNPQWVPKT LAEVTKADVE EFFQPVSDDL EWP

NT Sequence

GTGACGGATC AGATCGAAAC CCGGGTCGAG AAGGGGGTCG GCCTGCTGAC GCTGAACCGT CCCAAGGCCA TCAACTCGCT CACCTACGAC ATGATCGCGG CGATGAACGC CGTCCTGGCG GAGTGGGAGG CGGACGACGC GGTACGCGTG GTCGTGCTCT CGGGCGCCGG CGAGCGTGGT CTGTGCGCCG GCGGCGACGT GGTGGCCGTG CACGACAGCG CCAAGATCGA CGGCGTGGCA GCCCGCAGGT TCTTCCGCGA CGAGTACGTG CTCAACGCAC AGATCGCGCG CTTCCCGAAA CCGTATGTGG CGCTGATGGA CGGCATCGTG ATGGGCGGCG GTGTCGGAGT CGGCGGGCAT GCCAACATCC GCATTGTCAC CGATACCTCC AAGGTGGCGA TGCCGGAGGT GGGCATCGGC TTCATCCCGG ACGTGGGCGG TACCTACCTA CTCTCGCGCG CCCCCGGCCA GCTCGGCTTG CACGCCGCAC TCACGGGTGC ACCCTTCTCC GGAGCCGACG CCATCGCGCT GGGCTTCGCC GACCACTACG TGCCGCATGA CCGGCTCGAG AAGTTCACCA AGGCCATCAT CGATAGCGAT ATCGCCACTG CGCTTGCGGT CCACGGCACC AACGCACCGT CGAGTGATCT GCTGGCTCAA CGCAATTGGA TCGACGAGTG CTACGGCGGT GAATCCGTCG CCGATATCGT CGCCGCGCTG CAGGCCCGTG GTGACGAACG GGCACGCGCG GCAGCCGATC AAATCCTGAC CCGCTCCCCC ATCGCACTGT CGGTGACGCT TGCGGCAGTG CGCAGGGCTC GCGAACTGCC GTCGCTGGAA GCGGTTCTCA CCCAGGAATA TCGAGTGTCC AGCCAATCGG TGCGCTCGCA TGATCTGGTC GAGGGCATCA GGGCTCAGCT GGTCGACAAG GATCGCAACC CGCAGTGGGT TCCGAAGACA CTCGCCGAGG TCACCAAGGC CGATGTCGAG GAGTTCTTCC AGCCAGTCAG CGACGACTTG GAGTGGCCG

Details for MyabA.00340.a.A1.GE30290

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	93

Validated AA Sequence

MVTDQIETRV EKGVXLLTLN RPKXINSLTY DMIAAMNAVL AXWEXDXXVX XXVLSGAXXR GLCAGGDVVA VXXXAKIDGV AARRFFRXEY VLNAQIARFP KPXVALMDGI VMGGGVGVGG HANIRIVTDT SKVAMPEVGI GFIPDVGGTY LLSRAPGQLG LHAALTGAPF SGADAIALGF ADHYVPHDRL EKFTKAIIDS DIATALAVHG TNAPSSDLLA QRNWIDECYG GESVADIVAA LQARGDERAR AAADQILTRS PIALSVTLAA VRRARELPSL EAVLTQEYRV SSQSVRSHDL VEGIRAQLVD KDRNPQWVPK TLAEVTKADV EEFFQPVSDD LE

Validated NT Sequence

actccaagtc gtcgctgact ggctggaaga actcctcgac atcggccttg gtgacctcgg cgagtgtctt cggaacccac tgcgggttgc gatccttgtc gaccagctga gccctgatgc cctcgaccag atcatgcgag cgcaccgatt ggctggacac tcgatattcc tgggtgagaa ccgcttccag cgacggcagt tcgcgagccc tgcgcactgc cgcaagcgtc accgacagtg cgatggggga gcgggtcagg atttgatcgg ctgccgcgcg tgcccgttcg tcaccacggg cctgcagcgc ggcgacgata tcggcgacgg attcaccgcc gtagcactcg tcgatccaat tgcgttgagc cagcagatca ctcgacggtg cgttggtgcc gtggaccgca agcgcagtgg cgatatcgct atcgatgatg gccttggtga acttctcgag ccggtcatgc ggcacgtagt ggtcggcgaa gcccagcgcg atggcgtcgg ctccggagaa gggtgcaccc gtgagtgcgg cgtgcaagcc gagctggccg ggggcgcgcg agagtaggta ggtaccgccc acgtccggga tgaagccgat gcccacctcc ggcatcgcca ccttggaggt atcggtgaca atgcggatgt tggcatgccc gccgactccg acaccgccgc ccatcacgat gccgtccatc agcgccacat nnggtttcgg gaagcgcgcg atctgtgcgt tgagcacgta ctcntcgcgg aagaacctgc gggctgccac gccgtcgatc ttggcgcngt ngngcacggc caccacgtcg ccgccggcgc acagaccacg ctngcnggcg cccgagagca cgnccnnnng tacngngtng tcnncctccc antccgccag gacggcgttc atcgccgcga tcatgtcgta ggtgagcgag ttgatgncct tgggacggtt cagngtcagc aggcngaccc ccttctcgac ccgggtttcg atctgatccg tcaccatcga accng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTDQIETR VEKGVGLLTL NRPKAINSLT YDMIAAMNAV LAEWEADDAV RVVVLSGAGE RGLCAGGDVV AVHDSAKIDG VAARRFFRDE YVLNAQIARF PKPYVALMDG IVMGGGVGVG GHANIRIVTD TSKVAMPEVG IGFIPDVGGT YLLSRAPGQL GLHAALTGAP FSGADAIALG FADHYVPHDR LEKFTKAIID SDIATALAVH GTNAPSSDLL AQRNWIDECY GGESVADIVA ALQARGDERA RAAADQILTR SPIALSVTLA AVRRARELPS LEAVLTQEYR VSSQSVRSHD LVEGIRAQLV DKDRNPQWVP KTLAEVTKAD VEEFFQPVSD DLEWP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacggat cagatcgaaa cccgggtcga gaagggggtc ggcctgctga cgctgaaccg tcccaaggcc atcaactcgc tcacctacga catgatcgcg gcgatgaacg ccgtcctggc ggagtgggag gcggacgacg cggtacgcgt ggtcgtgctc tcgggcgccg gcgagcgtgg tctgtgcgcc ggcggcgacg tggtggccgt gcacgacagc gccaagatcg acggcgtggc agcccgcagg ttcttccgcg acgagtacgt gctcaacgca cagatcgcgc gcttcccgaa accgtatgtg gcgctgatgg acggcatcgt gatgggcggc ggtgtcggag tcggcgggca tgccaacatc cgcattgtca ccgatacctc caaggtggcg atgccggagg tgggcatcgg cttcatcccg gacgtgggcg gtacctacct actctcgcgc gcccccggcc agctcggctt gcacgccgca ctcacgggtg cacccttctc cggagccgac gccatcgcgc tgggcttcgc cgaccactac gtgccgcatg accggctcga gaagttcacc aaggccatca tcgatagcga tatcgccact gcgcttgcgg tccacggcac caacgcaccg tcgagtgatc tgctggctca acgcaattgg atcgacgagt gctacggcgg tgaatccgtc gccgatatcg tcgccgcgct gcaggcccgt ggtgacgaac gggcacgcgc ggcagccgat caaatcctga cccgctcccc catcgcactg tcggtgacgc ttgcggcagt gcgcagggct cgcgaactgc cgtcgctgga agcggttctc acccaggaat atcgagtgtc cagccaatcg gtgcgctcgc atgatctggt cgagggcatc agggctcagc tggtcgacaa ggatcgcaac ccgcagtggg ttccgaagac actcgccgag gtcaccaagg ccgatgtcga ggagttcttc cagccagtca gcgacgactt ggagtggccg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for MyabA.00340.a.A1.PS00893

PURIFICATION DATe:	11/30/2010
CONCENTRATION:	37.32mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGGAT CAGATCGAAA CCCGGGTCGA GAAGGGGGTC GGCCTGCTGA CGCTGAACCG TCCCAAGGCC ATCAACTCGC TCACCTACGA CATGATCGCG GCGATGAACG CCGTCCTGGC GGAGTGGGAG GCGGACGACG CGGTACGCGT GGTCGTGCTC TCGGGCGCCG GCGAGCGTGG TCTGTGCGCC GGCGGCGACG TGGTGGCCGT GCACGACAGC GCCAAGATCG ACGGCGTGGC AGCCCGCAGG TTCTTCCGCG ACGAGTACGT GCTCAACGCA CAGATCGCGC GCTTCCCGAA ACCGTATGTG GCGCTGATGG ACGGCATCGT GATGGGCGGC GGTGTCGGAG TCGGCGGGCA TGCCAACATC CGCATTGTCA CCGATACCTC CAAGGTGGCG ATGCCGGAGG TGGGCATCGG CTTCATCCCG GACGTGGGCG GTACCTACCT ACTCTCGCGC GCCCCCGGCC AGCTCGGCTT GCACGCCGCA CTCACGGGTG CACCCTTCTC CGGAGCCGAC GCCATCGCGC TGGGCTTCGC CGACCACTAC GTGCCGCATG ACCGGCTCGA GAAGTTCACC AAGGCCATCA TCGATAGCGA TATCGCCACT GCGCTTGCGG TCCACGGCAC CAACGCACCG TCGAGTGATC TGCTGGCTCA ACGCAATTGG ATCGACGAGT GCTACGGCGG TGAATCCGTC GCCGATATCG TCGCCGCGCT GCAGGCCCGT GGTGACGAAC GGGCACGCGC GGCAGCCGAT CAAATCCTGA CCCGCTCCCC CATCGCACTG TCGGTGACGC TTGCGGCAGT GCGCAGGGCT CGCGAACTGC CGTCGCTGGA AGCGGTTCTC ACCCAGGAAT ATCGAGTGTC CAGCCAATCG GTGCGCTCGC ATGATCTGGT CGAGGGCATC AGGGCTCAGC TGGTCGACAA GGATCGCAAC CCGCAGTGGG TTCCGAAGAC ACTCGCCGAG GTCACCAAGG CCGATGTCGA GGAGTTCTTC CAGCCAGTCA GCGACGACTT GGAGTGGCCG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA