SSGCID: Target Page

MyavA.00340.a

Enoyl-CoA hydratase

CENTER ID:	MyavA.00340.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00340.a.A1.GE28517	Full length( MyavA.00340.a )		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00340.a.A1.PS00588	Full length( MyavA.00340.a )		1	345

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1169
RefSeq:	YP_880442.1
UniProt:	A0A0H3A4G3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDGSDEVLT QVDGNVGLIT LNRPKAINSL NQPMIDALSA VLTDWARDDK VRAVLLSGAG ERGLCAGGDV VSIYHSARKD GVEARRFWRD EYQLNAQISE FAKPYVAVMD GIVMGGGVGV SAHANTRVVT DTSKVAMPEV GIGFIPDVGG VYLLSRAPGG LGLHAALTGA PFSGADAIAM GFADHYVPHA DVEAFRRAVV ADGVESALAK YAVEPPPGEL AAQRDWIDEC YAGQTVEDIV AALRAHGAGP AHDAADLIAT RSPIALSVTL AAVRRAADLP TLKDVLVQDY RVSSASLRSH DLVEGIRAQL IDKDRNPQWS PARLADVTAA DVEAYFAPVD DDLSF

NT Sequence

GTGACCGACG GATCCGACGA GGTTCTCACC CAAGTCGACG GAAACGTCGG CCTGATCACG CTCAACCGCC CCAAGGCGAT CAACTCGCTG AATCAGCCGA TGATCGACGC GCTGAGCGCC GTCCTCACCG ACTGGGCGCG CGACGACAAG GTGCGCGCGG TGCTGCTGTC CGGCGCCGGC GAGCGCGGGC TGTGCGCCGG CGGCGACGTG GTGTCCATCT ATCACAGCGC CCGCAAGGAC GGCGTCGAGG CGCGACGCTT CTGGCGCGAC GAATATCAGC TCAACGCCCA GATCTCGGAG TTCGCCAAGC CCTACGTCGC GGTGATGGAC GGCATCGTGA TGGGCGGCGG CGTCGGCGTC AGCGCGCACG CCAACACCCG GGTGGTCACC GACACCTCCA AGGTCGCCAT GCCCGAGGTC GGCATCGGGT TCATCCCCGA CGTCGGCGGC GTTTACCTGC TGTCCCGCGC GCCCGGCGGG CTCGGCCTGC ACGCCGCGCT GACCGGGGCG CCGTTCTCCG GCGCCGACGC CATCGCCATG GGATTCGCCG ACCACTACGT GCCGCACGCC GACGTCGAGG CGTTCCGCCG CGCGGTGGTC GCCGACGGCG TCGAGAGCGC GCTGGCCAAA TACGCCGTCG AGCCGCCACC CGGTGAGCTT GCGGCCCAAC GTGATTGGAT CGACGAGTGC TATGCGGGGC AGACCGTCGA GGACATCGTC GCCGCGCTGC GCGCGCATGG CGCGGGCCCG GCGCACGACG CCGCGGACCT GATCGCCACC CGCTCACCGA TCGCGCTGTC GGTGACCCTG GCGGCAGTGC GCCGAGCGGC CGACCTGCCG ACACTGAAAG ACGTTCTGGT GCAGGACTAT CGGGTGTCGT CGGCGTCGCT GCGCTCACAC GATCTGGTGG AGGGCATCCG GGCGCAGCTG ATCGACAAGG ACCGCAATCC GCAGTGGTCG CCCGCCCGGC TGGCCGACGT CACCGCGGCC GACGTCGAGG CGTATTTCGC CCCGGTGGAC GACGACTTGA GTTTC

Details for MyavA.00340.a.A1.GE28517

HARVESTED ON:	2/9/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	38kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTDGSDEV LTQVDGNVGL ITLNRPKAIN SLNQPMIDAL SAVLTDWARD DKVRAVLLSG AGERGLCAGG DVVSIYHSAR KDGVEARRFW RDEYQLNAQI SEFAKPYVAV MDGIVMGGGV GVSAHANTRV VTDTSKVAMP EVGIGFIPDV GGVYLLSRAP GGLGLHAALT GAPFSGADAI AMGFADHYVP HADVEAFRRA VVADGVESAL AKYAVEPPPG ELAAQRDWID ECYAGQTVED IVAALRAHGA GPAHDAADLI ATRSPIALSV TLAAVRRAAD LPTLKDVLVQ DYRVSSASLR SHDLVEGIRA QLIDKDRNPQ WSPARLADVT AADVEAYFAP VDDDLSF

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ccgacggatc cgacgaggtt ctcacccaag tcgacggaaa cgtcggcctg atcacgctca accgccccaa ggcgatcaac tcgctgaatc agccgatgat cgacgcgctg agcgccgtcc tcaccgactg ggcgcgcgac gacaaggtgc gcgcggtgct gctgtccggc gccggcgagc gcgggctgtg cgccggcggc gacgtggtgt ccatctatca cagcgcccgc aaggacggcg tcgaggcgcg acgcttctgg cgcgacgaat atcagctcaa cgcccagatc tcggagttcg ccaagcccta cgtcgcggtg atggacggca tcgtgatggg cggcggcgtc ggcgtcagcg cgcacgccaa cacccgggtg gtcaccgaca cctccaaggt cgccatgccc gaggtcggca tcgggttcat ccccgacgtc ggcggcgttt acctgctgtc ccgcgcgccc ggcgggctcg gcctgcacgc cgcgctgacc ggggcgccgt tctccggcgc cgacgccatc gccatgggat tcgccgacca ctacgtgccg cacgccgacg tcgaggcgtt ccgccgcgcg gtggtcgccg acggcgtcga gagcgcgctg gccaaatacg ccgtcgagcc gccacccggt gagcttgcgg cccaacgtga ttggatcgac gagtgctatg cggggcagac cgtcgaggac atcgtcgccg cgctgcgcgc gcatggcgcg ggcccggcgc acgacgccgc ggacctgatc gccacccgct caccgatcgc gctgtcggtg accctggcgg cagtgcgccg agcggccgac ctgccgacac tgaaagacgt tctggtgcag gactatcggg tgtcgtcggc gtcgctgcgc tcacacgatc tggtggaggg catccgggcg cagctgatcg acaaggaccg caatccgcag tggtcgcccg cccggctggc cgacgtcacc gcggccgacg tcgaggcgta tttcgccccg gtggacgacg acttgagttt ctaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgac ggatccgacg aggttctcac ccaagtcgac ggaaacgtcg gcctgatcac gctcaaccgc cccaaggcga tcaactcgct gaatcagccg atgatcgacg cgctgagcgc cgtcctcacc gactgggcgc gcgacgacaa ggtgcgcgcg gtgctgctgt ccggcgccgg cgagcgcggg ctgtgcgccg gcggcgacgt ggtgtccatc tatcacagcg cccgcaagga cggcgtcgag gcgcgacgct tctggcgcga cgaatatcag ctcaacgccc agatctcgga gttcgccaag ccctacgtcg cggtgatgga cggcatcgtg atgggcggcg gcgtcggcgt cagcgcgcac gccaacaccc gggtggtcac cgacacctcc aaggtcgcca tgcccgaggt cggcatcggg ttcatccccg acgtcggcgg cgtttacctg ctgtcccgcg cgcccggcgg gctcggcctg cacgccgcgc tgaccggggc gccgttctcc ggcgccgacg ccatcgccat gggattcgcc gaccactacg tgccgcacgc cgacgtcgag gcgttccgcc gcgcggtggt cgccgacggc gtcgagagcg cgctggccaa atacgccgtc gagccgccac ccggtgagct tgcggcccaa cgtgattgga tcgacgagtg ctatgcgggg cagaccgtcg aggacatcgt cgccgcgctg cgcgcgcatg gcgcgggccc ggcgcacgac gccgcggacc tgatcgccac ccgctcaccg atcgcgctgt cggtgaccct ggcggcagtg cgccgagcgg ccgacctgcc gacactgaaa gacgttctgg tgcaggacta tcgggtgtcg tcggcgtcgc tgcgctcaca cgatctggtg gagggcatcc gggcgcagct gatcgacaag gaccgcaatc cgcagtggtc gcccgcccgg ctggccgacg tcaccgcggc cgacgtcgag gcgtatttcg ccccggtgga cgacgacttg agtttcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for MyavA.00340.a.A1.PS00588

PURIFICATION DATe:	4/27/2010
CONCENTRATION:	51.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGAC GGATCCGACG AGGTTCTCAC CCAAGTCGAC GGAAACGTCG GCCTGATCAC GCTCAACCGC CCCAAGGCGA TCAACTCGCT GAATCAGCCG ATGATCGACG CGCTGAGCGC CGTCCTCACC GACTGGGCGC GCGACGACAA GGTGCGCGCG GTGCTGCTGT CCGGCGCCGG CGAGCGCGGG CTGTGCGCCG GCGGCGACGT GGTGTCCATC TATCACAGCG CCCGCAAGGA CGGCGTCGAG GCGCGACGCT TCTGGCGCGA CGAATATCAG CTCAACGCCC AGATCTCGGA GTTCGCCAAG CCCTACGTCG CGGTGATGGA CGGCATCGTG ATGGGCGGCG GCGTCGGCGT CAGCGCGCAC GCCAACACCC GGGTGGTCAC CGACACCTCC AAGGTCGCCA TGCCCGAGGT CGGCATCGGG TTCATCCCCG ACGTCGGCGG CGTTTACCTG CTGTCCCGCG CGCCCGGCGG GCTCGGCCTG CACGCCGCGC TGACCGGGGC GCCGTTCTCC GGCGCCGACG CCATCGCCAT GGGATTCGCC GACCACTACG TGCCGCACGC CGACGTCGAG GCGTTCCGCC GCGCGGTGGT CGCCGACGGC GTCGAGAGCG CGCTGGCCAA ATACGCCGTC GAGCCGCCAC CCGGTGAGCT TGCGGCCCAA CGTGATTGGA TCGACGAGTG CTATGCGGGG CAGACCGTCG AGGACATCGT CGCCGCGCTG CGCGCGCATG GCGCGGGCCC GGCGCACGAC GCCGCGGACC TGATCGCCAC CCGCTCACCG ATCGCGCTGT CGGTGACCCT GGCGGCAGTG CGCCGAGCGG CCGACCTGCC GACACTGAAA GACGTTCTGG TGCAGGACTA TCGGGTGTCG TCGGCGTCGC TGCGCTCACA CGATCTGGTG GAGGGCATCC GGGCGCAGCT GATCGACAAG GACCGCAATC CGCAGTGGTC GCCCGCCCGG CTGGCCGACG TCACCGCGGC CGACGTCGAG GCGTATTTCG CCCCGGTGGA CGACGACTTG AGTTTCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA