MythA.01530.a
3-hydroxyisobutyryl-CoA hydrolase
| CENTER ID: | MythA.01530.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01530.a.A1.GE28788 | Full length( MythA.01530.a ) | 1 | 351 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01530.a.A1.PW28690 | Full length( MythA.01530.a ) | 1 | 351 |
Structures
4HDT
DEPOSITED: 10/2/2012
DETERMINATION: XRay
CLONE: MythA.01530.a.A1.GE28788
PROTEIN: MythA.01530.a.A1.PW28690
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.585 |
| RefSeq: | WP_003924040.1 |
| UniProt: | G7CC99 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAKNEDVLV NVEGGVGLLT LNRPKAINSL THGMVTTMAE RLAAWENDDS VRAVLLTGAG ERGLCAGGDV VAIYHSAKAD GAEARRFWFD EYRLNAHIGR YPKPYVSIMD GIVMGGGVGV GAHGNVRVVT DTTKMAMPEV GIGFIPDVGG TYLLSRAPGK LGLHAALTGA PFSGADAIVM GFADHYVPHD KIDEFTRAVI ADGVDAALAA HAQEPPASPL AEQRSWIDEC YTGDTVADII AALRAHDAPA AGEAADLIAT RSPIALSVTL ESVRRAAKLQ SLEDTLRQEY RVSCASLKSH DLVEGIRAQL VDKDRNPKWR PATLAEVTEA DVEAYFAPVD PELTFEGETQ
NT Sequence
GTGACCGCCA AAAACGAGGA TGTCCTAGTA AATGTTGAGG GCGGTGTCGG GCTGCTGACC CTCAACCGGC CGAAAGCCAT CAACTCGCTC ACCCACGGCA TGGTGACGAC GATGGCCGAG CGCCTGGCCG CGTGGGAGAA CGACGACTCG GTGCGCGCCG TGCTGCTCAC CGGGGCCGGG GAACGCGGGT TGTGCGCCGG CGGTGACGTG GTGGCGATCT ACCACAGCGC CAAGGCCGAC GGCGCCGAGG CCCGCCGGTT CTGGTTCGAC GAGTACCGGC TCAACGCGCA CATCGGCCGC TACCCGAAGC CGTACGTCTC GATCATGGAC GGCATCGTGA TGGGCGGCGG CGTCGGAGTG GGCGCGCACG GCAATGTCCG GGTCGTCACC GACACCACCA AGATGGCGAT GCCCGAGGTC GGTATCGGCT TCATTCCCGA CGTCGGCGGC ACCTACCTGC TGTCCCGGGC GCCCGGCAAG CTCGGCCTGC ACGCGGCGCT GACCGGTGCG CCGTTCTCCG GGGCGGACGC GATCGTGATG GGCTTCGCCG ACCACTATGT GCCGCACGAC AAGATCGACG AGTTCACCCG CGCGGTGATC GCCGACGGTG TGGACGCCGC GCTCGCCGCG CACGCCCAGG AGCCGCCGGC CAGCCCGCTG GCCGAGCAGC GGTCCTGGAT CGACGAGTGC TACACGGGTG ACACCGTCGC CGACATCATC GCGGCGCTGC GTGCGCACGA CGCCCCGGCG GCCGGGGAGG CGGCGGATCT GATCGCCACC CGCTCCCCGA TCGCGCTGTC GGTGACGCTG GAGTCGGTGC GACGGGCCGC GAAGCTCCAG AGCCTCGAAG ACACGTTGCG CCAGGAGTAC CGGGTCTCGT GTGCGTCGCT GAAGTCGCAC GACCTGGTCG AGGGCATCCG GGCGCAGCTC GTCGACAAGG ACCGCAACCC CAAGTGGCGG CCGGCCACCC TGGCCGAGGT CACCGAAGCC GACGTCGAGG CCTATTTCGC CCCGGTCGAC CCCGAGCTGA CTTTCGAAGG AGAAACACAA TGA
Details for MythA.01530.a.A1.GE28788
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 3/5/2010 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 67 |
Validated AA Sequence
MGTLEAQTQG PGSMVTAKNE DVLVNVEGGV GLLTLNRPKA INSLTHGMVT TMAERLAAWE NDDSVRAVLL TGAGERGLCA GGDVVAIYHS AKADGAEARR FWFDEYRLNA HIGRYPKPYV SIMDGIVMGG GVGVGAHGNV RVVTDTTKMA MPEVGIGFIP DVGGTYLLSR APGKLGLHAA LTGAPFSGAD AIVMGFADHY VPHDKIDEFT RAVIADGVDA ALAAHAQEPP AXPLAXQRSW XDXCYTGDXV A
Validated NT Sequence
catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gtgaccgcca aaaacgagga tgtcctagta aatgttgagg gcggtgtcgg gctgctgacc ctcaaccggc cgaaagccat caactcgctc acccacggca tggtgacgac gatggccgag cgcctggccg cgtgggagaa cgacgactcg gtgcgcgccg tgctgctcac cggggccggg gaacgcgggt tgtgcgccgg cggtgacgtg gtggcgatct accacagcgc caaggccgac ggcgccgagg cccgccggtt ctggttcgac gagtaccggc tcaacgcgca catcggccgc tacccgaagc cgtacgtctc gatcatggac ggcatcgtga tgggcggcgg cgtcggagtg ggcgcgcacg gcaatgtccg ggtcgtcacc gacaccacca agatggcgat gcccgaggtc ggtatcggct tcattcccga cgtcggcggc acctacctgc tgtcccgggc gcccggcaag ctcggcctgc acgcggcgct gaccggtgcg ccgttctccg gggcggacgc gatcgtgatg ggcttcgccg accactatgt gccgcacgac aagatcgacg agttcacccg cgcggtgatc gccgacggtg tggacgccgc gctcgccgcg cacgcccagg agccgccggc cagnccgctg gccgancagc ggtcctggnt cgacnantgc tacacgggtg acnccgtcgc cna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAKNEDV LVNVEGGVGL LTLNRPKAIN SLTHGMVTTM AERLAAWEND DSVRAVLLTG AGERGLCAGG DVVAIYHSAK ADGAEARRFW FDEYRLNAHI GRYPKPYVSI MDGIVMGGGV GVGAHGNVRV VTDTTKMAMP EVGIGFIPDV GGTYLLSRAP GKLGLHAALT GAPFSGADAI VMGFADHYVP HDKIDEFTRA VIADGVDAAL AAHAQEPPAS PLAEQRSWID ECYTGDTVAD IIAALRAHDA PAAGEAADLI ATRSPIALSV TLESVRRAAK LQSLEDTLRQ EYRVSCASLK SHDLVEGIRA QLVDKDRNPK WRPATLAEVT EADVEAYFAP VDPELTFEGE TQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgcc aaaaacgagg atgtcctagt aaatgttgag ggcggtgtcg ggctgctgac cctcaaccgg ccgaaagcca tcaactcgct cacccacggc atggtgacga cgatggccga gcgcctggcc gcgtgggaga acgacgactc ggtgcgcgcc gtgctgctca ccggggccgg ggaacgcggg ttgtgcgccg gcggtgacgt ggtggcgatc taccacagcg ccaaggccga cggcgccgag gcccgccggt tctggttcga cgagtaccgg ctcaacgcgc acatcggccg ctacccgaag ccgtacgtct cgatcatgga cggcatcgtg atgggcggcg gcgtcggagt gggcgcgcac ggcaatgtcc gggtcgtcac cgacaccacc aagatggcga tgcccgaggt cggtatcggc ttcattcccg acgtcggcgg cacctacctg ctgtcccggg cgcccggcaa gctcggcctg cacgcggcgc tgaccggtgc gccgttctcc ggggcggacg cgatcgtgat gggcttcgcc gaccactatg tgccgcacga caagatcgac gagttcaccc gcgcggtgat cgccgacggt gtggacgccg cgctcgccgc gcacgcccag gagccgccgg ccagcccgct ggccgagcag cggtcctgga tcgacgagtg ctacacgggt gacaccgtcg ccgacatcat cgcggcgctg cgtgcgcacg acgccccggc ggccggggag gcggcggatc tgatcgccac ccgctccccg atcgcgctgt cggtgacgct ggagtcggtg cgacgggccg cgaagctcca gagcctcgaa gacacgttgc gccaggagta ccgggtctcg tgtgcgtcgc tgaagtcgca cgacctggtc gagggcatcc gggcgcagct cgtcgacaag gaccgcaacc ccaagtggcg gccggccacc ctggccgagg tcaccgaagc cgacgtcgag gcctatttcg ccccggtcga ccccgagctg actttcgaag gagaaacaca atgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MythA.01530.a.A1.PW28690
| PURIFICATION DATe: | 6/21/2010 |
| CONCENTRATION: | 29.21mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 17 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 67 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVTAKNE DVLVNVEGGV GLLTLNRPKA INSLTHGMVT TMAERLAAWE NDDSVRAVLL TGAGERGLCA GGDVVAIYHS AKADGAEARR FWFDEYRLNA HIGRYPKPYV SIMDGIVMGG GVGVGAHGNV RVVTDTTKMA MPEVGIGFIP DVGGTYLLSR APGKLGLHAA LTGAPFSGAD AIVMGFADHY VPHDKIDEFT RAVIADGVDA ALAAHAQEPP AXPLAXQRSW XDXCYTGDXV A
Validated NT Sequence
catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gtgaccgcca aaaacgagga tgtcctagta aatgttgagg gcggtgtcgg gctgctgacc ctcaaccggc cgaaagccat caactcgctc acccacggca tggtgacgac gatggccgag cgcctggccg cgtgggagaa cgacgactcg gtgcgcgccg tgctgctcac cggggccggg gaacgcgggt tgtgcgccgg cggtgacgtg gtggcgatct accacagcgc caaggccgac ggcgccgagg cccgccggtt ctggttcgac gagtaccggc tcaacgcgca catcggccgc tacccgaagc cgtacgtctc gatcatggac ggcatcgtga tgggcggcgg cgtcggagtg ggcgcgcacg gcaatgtccg ggtcgtcacc gacaccacca agatggcgat gcccgaggtc ggtatcggct tcattcccga cgtcggcggc acctacctgc tgtcccgggc gcccggcaag ctcggcctgc acgcggcgct gaccggtgcg ccgttctccg gggcggacgc gatcgtgatg ggcttcgccg accactatgt gccgcacgac aagatcgacg agttcacccg cgcggtgatc gccgacggtg tggacgccgc gctcgccgcg cacgcccagg agccgccggc cagnccgctg gccgancagc ggtcctggnt cgacnantgc tacacgggtg acnccgtcgc cna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAKNEDV LVNVEGGVGL LTLNRPKAIN SLTHGMVTTM AERLAAWEND DSVRAVLLTG AGERGLCAGG DVVAIYHSAK ADGAEARRFW FDEYRLNAHI GRYPKPYVSI MDGIVMGGGV GVGAHGNVRV VTDTTKMAMP EVGIGFIPDV GGTYLLSRAP GKLGLHAALT GAPFSGADAI VMGFADHYVP HDKIDEFTRA VIADGVDAAL AAHAQEPPAS PLAEQRSWID ECYTGDTVAD IIAALRAHDA PAAGEAADLI ATRSPIALSV TLESVRRAAK LQSLEDTLRQ EYRVSCASLK SHDLVEGIRA QLVDKDRNPK WRPATLAEVT EADVEAYFAP VDPELTFEGE TQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGCC AAAAACGAGG ATGTCCTAGT AAATGTTGAG GGCGGTGTCG GGCTGCTGAC CCTCAACCGG CCGAAAGCCA TCAACTCGCT CACCCACGGC ATGGTGACGA CGATGGCCGA GCGCCTGGCC GCGTGGGAGA ACGACGACTC GGTGCGCGCC GTGCTGCTCA CCGGGGCCGG GGAACGCGGG TTGTGCGCCG GCGGTGACGT GGTGGCGATC TACCACAGCG CCAAGGCCGA CGGCGCCGAG GCCCGCCGGT TCTGGTTCGA CGAGTACCGG CTCAACGCGC ACATCGGCCG CTACCCGAAG CCGTACGTCT CGATCATGGA CGGCATCGTG ATGGGCGGCG GCGTCGGAGT GGGCGCGCAC GGCAATGTCC GGGTCGTCAC CGACACCACC AAGATGGCGA TGCCCGAGGT CGGTATCGGC TTCATTCCCG ACGTCGGCGG CACCTACCTG CTGTCCCGGG CGCCCGGCAA GCTCGGCCTG CACGCGGCGC TGACCGGTGC GCCGTTCTCC GGGGCGGACG CGATCGTGAT GGGCTTCGCC GACCACTATG TGCCGCACGA CAAGATCGAC GAGTTCACCC GCGCGGTGAT CGCCGACGGT GTGGACGCCG CGCTCGCCGC GCACGCCCAG GAGCCGCCGG CCAGCCCGCT GGCCGAGCAG CGGTCCTGGA TCGACGAGTG CTACACGGGT GACACCGTCG CCGACATCAT CGCGGCGCTG CGTGCGCACG ACGCCCCGGC GGCCGGGGAG GCGGCGGATC TGATCGCCAC CCGCTCCCCG ATCGCGCTGT CGGTGACGCT GGAGTCGGTG CGACGGGCCG CGAAGCTCCA GAGCCTCGAA GACACGTTGC GCCAGGAGTA CCGGGTCTCG TGTGCGTCGC TGAAGTCGCA CGACCTGGTC GAGGGCATCC GGGCGCAGCT CGTCGACAAG GACCGCAACC CCAAGTGGCG GCCGGCCACC CTGGCCGAGG TCACCGAAGC CGACGTCGAG GCCTATTTCG CCCCGGTCGA CCCCGAGCTG ACTTTCGAAG GAGAAACACA ATGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA