MytuD.00200.a
Arginine/ornithine transport system ATPase
| CENTER ID: | MytuD.00200.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00200.a.A1.GE25813 | Full length( MytuD.00200.a ) | 1 | 334 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00200.a.A1.PW25862 | Full length( MytuD.00200.a ) | 1 | 334 |
Structures
3MD0
DEPOSITED: 3/29/2010
DETERMINATION: XRay
CLONE: MytuD.00200.a.A1.GE25813
PROTEIN: MytuD.00200.a.A1.PW25862
3P32
DEPOSITED: 10/4/2010
DETERMINATION: XRay
CLONE: MytuD.00200.a.A1.GE25813
PROTEIN: MytuD.00200.a.A1.PW25862
4GT1
DEPOSITED: 8/28/2012
DETERMINATION: XRay
CLONE: MytuD.00200.a.A1.GE25813
PROTEIN: MytuD.00200.a.A1.PW25862
Publications by SSGCID
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Abendroth J, Baugh L, Baydo RO, Bullen J, Clifton MC, Edwards TE, Grundner C, Lorimer DD, Myler PJ, Phan IQ, Sankaran B, Staker BL, Thompkins K, Van Voorhis WC, Witte P
J. Struct. Funct. Genomics - 2015
volume 16, issue 2, pages 91-9
PMID: 25832174; PMCID: PMC4631608
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131892 |
| PATRIC ID: | fig|83332.12.peg.1670 |
| RefSeq: | NP_216012.1 |
| TubercuList: | Rv1496 |
| MTB Network Portal: | Rv1496 |
| UniProt: | P9WPZ1 |
Orthologues for Rv1496
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| marinum | 85 | 98 | 0 | 0 | 0 |
| ulcerans | 84 | 98 | 1 | 0 | 0 |
| leprae | 83 | 97 | 1 | 0 | 0 |
| avium | 83 | 96 | 0 | 0 | 0 |
| paratuberculosis | 83 | 96 | 1 | 0 | 0 |
| smegmatis | 80 | 96 | 1 | 1 | 1 |
| abscessus | 78 | 96 | 0 | 0 | 0 |
| thermoresistibile | 61 | 96 | 1 | 1 | 1 |
| smegmatis | 40 | 85 | 1 | 1 | 1 |
| marinum | 40 | 76 | 1 | 0 | 0 |
| ulcerans | 39 | 76 | 1 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMAASHDDDT VDGLATAVRG GDRAALPRAI TLVESTRPDH REQAQQLLLR LLPDSGNAHR VGITGVPGVG KSTAIEALGM HLIERGHRVA VLAVDPSSTR TGGSILGDKT RMARLAVHPN AYIRPSPTSG TLGGVTRATR ETVVLLEAAG FDVILIETVG VGQSEVAVAN MVDTFVLLTL ARTGDQLQGI KKGVLELADI VVVNKADGEH HKEARLAARE LSAAIRLIYP REALWRPPVL TMSAVEGRGL AELWDTVERH RQVLTGAGEF DARRRDQQVD WTWQLVRDAV LDRVWSNPTV RKVRSELERR VRAGELTPAL AAQQILEIAN LTDR
NT Sequence
ATGATGGCCG CATCCCACGA CGACGACACC GTCGACGGGT TGGCGACGGC CGTGCGCGGC GGTGACCGTG CGGCGCTGCC ACGGGCCATC ACACTGGTCG AGTCGACCCG CCCCGACCAT CGTGAGCAGG CGCAACAGCT GCTGCTGCGA TTGCTGCCGG ACTCCGGGAA CGCCCATCGC GTCGGCATCA CCGGGGTCCC GGGGGTGGGC AAGTCGACTG CCATCGAGGC GCTGGGCATG CATCTGATCG AGCGCGGGCA TCGGGTGGCG GTGCTGGCGG TCGACCCGTC GTCGACCCGC ACGGGTGGAT CGATTCTTGG TGATAAAACC CGGATGGCGC GGCTGGCGGT GCACCCGAAC GCCTACATCC GGCCGTCCCC GACGTCGGGA ACGCTGGGTG GGGTGACGAG GGCCACCCGG GAAACGGTGG TGCTGTTGGA GGCGGCCGGT TTTGATGTGA TCCTGATCGA AACCGTCGGG GTGGGCCAGT CCGAGGTCGC GGTGGCCAAC ATGGTCGACA CGTTCGTGTT GCTGACCTTG GCCCGCACCG GTGATCAGTT GCAGGGCATC AAGAAGGGCG TGCTGGAGCT CGCCGACATC GTGGTGGTGA ACAAGGCCGA CGGGGAGCAC CACAAAGAGG CCCGGCTGGC CGCCCGGGAG CTGTCGGCGG CGATCAGATT GATCTATCCT CGCGAAGCAC TGTGGCGCCC ACCGGTGCTC ACCATGAGCG CGGTGGAGGG CAGGGGACTG GCCGAGCTGT GGGACACCGT CGAGCGTCAT CGCCAGGTGC TCACCGGGGC CGGCGAATTC GACGCCCGTC GGCGCGATCA GCAGGTCGAC TGGACCTGGC AGCTGGTTCG CGACGCCGTC CTGGATCGGG TGTGGTCCAA TCCGACGGTG CGCAAGGTCC GCTCCGAGCT CGAGCGTCGG GTCCGCGCCG GCGAACTGAC CCCGGCCCTG GCGGCTCAGC AAATACTGGA GATAGCTAAC CTAACGGATA GG
Details for MytuD.00200.a.A1.GE25813
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMMAASHD DDTVDGLATA VRGGDRAALP RAITLVESTR PDHREQAQQL LLRLLPDSGN AHRVGITGVP GVGKSTAIEA LGMHLIERGH RVAVLAVDPS STRTGGSILG DKTRMARLAV HPNAYIRPSP TSGTLGGVTR ATRETVVLLE AAGFDVILIE TVGVGQSEVA VANMVDTFVL LTLARTGDQL QGIKKGVLEL ADIVVVNKAD GEHHKEARLA ARELSAAIRL IYPREALWRP PVLTMSAVEG RGLAELWDTV ERHRQVLTGA GEFDARRRDQ QVDWTWQLVR DAVLDRVWSN PTVRKVRSEL ERRVRAGELT PALAAQQILE IANLT
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatggc cgcatcccac gacgacgaca ccgtcgacgg gttggcgacg gccgtgcgcg gcggtgaccg tgcggcgctg ccacgggcca tcacactggt cgagtcgacc cgccccgacc atcgtgagca ggcgcaacag ctgctgctgc gattgctgcc ggactccggg aacgcccatc gcgtcggcat caccggggtc ccgggggtgg gcaagtcgac tgccatcgag gcgctgggca tgcatctgat cgagcgcggg catcgggtgg cggtgctggc ggtcgacccg tcgtcgaccc gcacgggtgg atcgattctt ggtgataaaa cccggatggc gcggctggcg gtgcacccga acgcctacat ccggccgtcc ccgacgtcgg gaacgctggg tggggtgacg agggccaccc gggaaacggt ggtgctgttg gaggcggccg gttttgatgt gatcctgatc gaaaccgtcg gggtgggcca gtccgaggtc gcggtggcca acatggtcga cacgttcgtg ttgctgacct tggcccgcac cggtgatcag ttgcagggca tcaagaaggg cgtgctggag ctcgccgaca tcgtggtggt gaacaaggcc gacggggagc accacaaaga ggcccggctg gccgcccggg agctgtcggc ggcgatcaga ttgatctatc ctcgcgaagc actgtggcgc ccaccggtgc tcaccatgag cgcggtggag ggcaggggac tggccgagct gtgggacacc gtcgagcgtc atcgccaggt gctcaccggg gccggcgaat tcgacgcccg tcggcgcgat cagcaggtcg actggacctg gcagctggtt cgcgacgccg tcctggatcg ggtgtggtcc aatccgacgg tgcgcaaggt ccgctccgag ctcgagcgtc gggtccgcgc cggcgaactg accccggccc tggcggctca gcaaatactg gagatagcta acctaacgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMAASHDDD TVDGLATAVR GGDRAALPRA ITLVESTRPD HREQAQQLLL RLLPDSGNAH RVGITGVPGV GKSTAIEALG MHLIERGHRV AVLAVDPSST RTGGSILGDK TRMARLAVHP NAYIRPSPTS GTLGGVTRAT RETVVLLEAA GFDVILIETV GVGQSEVAVA NMVDTFVLLT LARTGDQLQG IKKGVLELAD IVVVNKADGE HHKEARLAAR ELSAAIRLIY PREALWRPPV LTMSAVEGRG LAELWDTVER HRQVLTGAGE FDARRRDQQV DWTWQLVRDA VLDRVWSNPT VRKVRSELER RVRAGELTPA LAAQQILEIA NLTDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatggccgca tcccacgacg acgacaccgt cgacgggttg gcgacggccg tgcgcggcgg tgaccgtgcg gcgctgccac gggccatcac actggtcgag tcgacccgcc ccgaccatcg tgagcaggcg caacagctgc tgctgcgatt gctgccggac tccgggaacg cccatcgcgt cggcatcacc ggggtcccgg gggtgggcaa gtcgactgcc atcgaggcgc tgggcatgca tctgatcgag cgcgggcatc gggtggcggt gctggcggtc gacccgtcgt cgacccgcac gggtggatcg attcttggtg ataaaacccg gatggcgcgg ctggcggtgc acccgaacgc ctacatccgg ccgtccccga cgtcgggaac gctgggtggg gtgacgaggg ccacccggga aacggtggtg ctgttggagg cggccggttt tgatgtgatc ctgatcgaaa ccgtcggggt gggccagtcc gaggtcgcgg tggccaacat ggtcgacacg ttcgtgttgc tgaccttggc ccgcaccggt gatcagttgc agggcatcaa gaagggcgtg ctggagctcg ccgacatcgt ggtggtgaac aaggccgacg gggagcacca caaagaggcc cggctggccg cccgggagct gtcggcggcg atcagattga tctatcctcg cgaagcactg tggcgcccac cggtgctcac catgagcgcg gtggagggca ggggactggc cgagctgtgg gacaccgtcg agcgtcatcg ccaggtgctc accggggccg gcgaattcga cgcccgtcgg cgcgatcagc aggtcgactg gacctggcag ctggttcgcg acgccgtcct ggatcgggtg tggtccaatc cgacggtgcg caaggtccgc tccgagctcg agcgtcgggt ccgcgccggc gaactgaccc cggccctggc ggctcagcaa atactggaga tagctaacct aacggatagg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MytuD.00200.a.A1.PW25862
| PURIFICATION DATe: | 1/20/2009 |
| CONCENTRATION: | 22.01mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMMAASHD DDTVDGLATA VRGGDRAALP RAITLVESTR PDHREQAQQL LLRLLPDSGN AHRVGITGVP GVGKSTAIEA LGMHLIERGH RVAVLAVDPS STRTGGSILG DKTRMARLAV HPNAYIRPSP TSGTLGGVTR ATRETVVLLE AAGFDVILIE TVGVGQSEVA VANMVDTFVL LTLARTGDQL QGIKKGVLEL ADIVVVNKAD GEHHKEARLA ARELSAAIRL IYPREALWRP PVLTMSAVEG RGLAELWDTV ERHRQVLTGA GEFDARRRDQ QVDWTWQLVR DAVLDRVWSN PTVRKVRSEL ERRVRAGELT PALAAQQILE IANLT
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatggc cgcatcccac gacgacgaca ccgtcgacgg gttggcgacg gccgtgcgcg gcggtgaccg tgcggcgctg ccacgggcca tcacactggt cgagtcgacc cgccccgacc atcgtgagca ggcgcaacag ctgctgctgc gattgctgcc ggactccggg aacgcccatc gcgtcggcat caccggggtc ccgggggtgg gcaagtcgac tgccatcgag gcgctgggca tgcatctgat cgagcgcggg catcgggtgg cggtgctggc ggtcgacccg tcgtcgaccc gcacgggtgg atcgattctt ggtgataaaa cccggatggc gcggctggcg gtgcacccga acgcctacat ccggccgtcc ccgacgtcgg gaacgctggg tggggtgacg agggccaccc gggaaacggt ggtgctgttg gaggcggccg gttttgatgt gatcctgatc gaaaccgtcg gggtgggcca gtccgaggtc gcggtggcca acatggtcga cacgttcgtg ttgctgacct tggcccgcac cggtgatcag ttgcagggca tcaagaaggg cgtgctggag ctcgccgaca tcgtggtggt gaacaaggcc gacggggagc accacaaaga ggcccggctg gccgcccggg agctgtcggc ggcgatcaga ttgatctatc ctcgcgaagc actgtggcgc ccaccggtgc tcaccatgag cgcggtggag ggcaggggac tggccgagct gtgggacacc gtcgagcgtc atcgccaggt gctcaccggg gccggcgaat tcgacgcccg tcggcgcgat cagcaggtcg actggacctg gcagctggtt cgcgacgccg tcctggatcg ggtgtggtcc aatccgacgg tgcgcaaggt ccgctccgag ctcgagcgtc gggtccgcgc cggcgaactg accccggccc tggcggctca gcaaatactg gagatagcta acctaacgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMAASHDDD TVDGLATAVR GGDRAALPRA ITLVESTRPD HREQAQQLLL RLLPDSGNAH RVGITGVPGV GKSTAIEALG MHLIERGHRV AVLAVDPSST RTGGSILGDK TRMARLAVHP NAYIRPSPTS GTLGGVTRAT RETVVLLEAA GFDVILIETV GVGQSEVAVA NMVDTFVLLT LARTGDQLQG IKKGVLELAD IVVVNKADGE HHKEARLAAR ELSAAIRLIY PREALWRPPV LTMSAVEGRG LAELWDTVER HRQVLTGAGE FDARRRDQQV DWTWQLVRDA VLDRVWSNPT VRKVRSELER RVRAGELTPA LAAQQILEIA NLTDR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGGCCGCA TCCCACGACG ACGACACCGT CGACGGGTTG GCGACGGCCG TGCGCGGCGG TGACCGTGCG GCGCTGCCAC GGGCCATCAC ACTGGTCGAG TCGACCCGCC CCGACCATCG TGAGCAGGCG CAACAGCTGC TGCTGCGATT GCTGCCGGAC TCCGGGAACG CCCATCGCGT CGGCATCACC GGGGTCCCGG GGGTGGGCAA GTCGACTGCC ATCGAGGCGC TGGGCATGCA TCTGATCGAG CGCGGGCATC GGGTGGCGGT GCTGGCGGTC GACCCGTCGT CGACCCGCAC GGGTGGATCG ATTCTTGGTG ATAAAACCCG GATGGCGCGG CTGGCGGTGC ACCCGAACGC CTACATCCGG CCGTCCCCGA CGTCGGGAAC GCTGGGTGGG GTGACGAGGG CCACCCGGGA AACGGTGGTG CTGTTGGAGG CGGCCGGTTT TGATGTGATC CTGATCGAAA CCGTCGGGGT GGGCCAGTCC GAGGTCGCGG TGGCCAACAT GGTCGACACG TTCGTGTTGC TGACCTTGGC CCGCACCGGT GATCAGTTGC AGGGCATCAA GAAGGGCGTG CTGGAGCTCG CCGACATCGT GGTGGTGAAC AAGGCCGACG GGGAGCACCA CAAAGAGGCC CGGCTGGCCG CCCGGGAGCT GTCGGCGGCG ATCAGATTGA TCTATCCTCG CGAAGCACTG TGGCGCCCAC CGGTGCTCAC CATGAGCGCG GTGGAGGGCA GGGGACTGGC CGAGCTGTGG GACACCGTCG AGCGTCATCG CCAGGTGCTC ACCGGGGCCG GCGAATTCGA CGCCCGTCGG CGCGATCAGC AGGTCGACTG GACCTGGCAG CTGGTTCGCG ACGCCGTCCT GGATCGGGTG TGGTCCAATC CGACGGTGCG CAAGGTCCGC TCCGAGCTCG AGCGTCGGGT CCGCGCCGGC GAACTGACCC CGGCCCTGGC GGCTCAGCAA ATACTGGAGA TAGCTAACCT AACGGATAGG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA