MyleA.00200.a

Putative kinase

CENTER ID: MyleA.00200.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00200.a.A1.GE29808 Full length( MyleA.00200.a ) 1 327
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_131892
PATRIC ID: fig|561304.4.peg.3436
RefSeq: YP_002503850.1
UniProt: A0A0H3MVQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGDSTVDRL VAAVRSGDRV ALPRAITLVE STRSDHREQA QQLLLALQPD AGNAHRVGIT GVPGVGKSTA IEALGMHLIE RGHRVAVLAV DPSSVRTGGS ILGDKTRMAR LAVHPDAYIR PSPTSGTLGG VTKATRETVV LLEAAGFDVI LIETVGVGQS EVAVANMVDT FVLLALARTG DQLQGIKKGV LELADIVVVN KADGEHLRDA RLAARDLSSA LRLIYTHEKI WRPKVFTISA TEGTGLTQMW EAVECHRQVL TASGEFENRR LAQQVEWAWQ MVRDAVLDRV LSNPAVREIR AELECQVRAG ELTPAMAAQQ ILQAASC
NT Sequence
ATGGCGGGGG ACAGCACCGT GGACAGACTA GTCGCTGCTG TCCGTAGTGG TGATCGCGTG GCATTGCCGC GGGCTATAAC GTTGGTGGAG TCCACTCGCT CCGACCATCG TGAGCAGGCG CAGCAGCTGC TGCTGGCATT GCAACCGGAT GCTGGAAACG CCCATCGGGT GGGCATCACC GGGGTGCCCG GGGTAGGAAA GTCCACCGCC ATTGAGGCGC TTGGCATGCA TCTGATCGAA CGGGGTCATC GAGTGGCGGT GCTGGCCGTC GACCCATCAT CGGTCCGTAC CGGTGGATCC ATCCTTGGCG ACAAGACCCG GATGGCGCGG CTAGCGGTAC ACCCGGACGC CTATATCCGG CCATCGCCGA CATCGGGCAC GTTGGGCGGG GTGACGAAGG CCACTCGGGA AACAGTTGTC CTGTTGGAGG CGGCTGGTTT TGATGTAATT CTGATCGAAA CTGTAGGCGT CGGACAGTCT GAGGTAGCGG TGGCTAATAT GGTCGACACC TTCGTTTTGT TGGCCTTGGC GCGTACCGGC GATCAGTTGC AGGGCATCAA AAAAGGCGTG CTGGAGCTGG CCGACATCGT GGTGGTGAAC AAGGCCGATG GGGAGCATCT GCGCGATGCT CGGCTAGCTG CTCGGGACCT GTCATCGGCA CTCCGATTGA TCTATACACA CGAAAAAATT TGGAGACCAA AAGTTTTCAC GATTAGTGCG ACTGAGGGGA CCGGTCTGAC CCAGATGTGG GAAGCGGTCG AATGTCATCG TCAAGTGCTT ACTGCCTCCG GCGAGTTTGA GAACCGTCGA CTGGCCCAGC AAGTCGAGTG GGCTTGGCAG ATGGTCCGTG ACGCGGTGCT AGACCGGGTG CTGTCCAACC CCGCGGTGCG TGAGATTCGT GCTGAGCTGG AGTGTCAGGT GCGAGCAGGG GAGCTGACTC CCGCGATGGC GGCCCAGCAA ATCCTGCAGG CAGCATCCTG C
Details for MyleA.00200.a.A1.GE29808
HARVESTED ON: 5/27/2010
SEQUENCED ON: 8/31/2010
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 3
PERCENT COVERAGE: 51
Validated AA Sequence
MXDTFXXXXX XRXXIXXMXX XXXXXXXXTX XXXXXPXXXX XXXXXXXXCF XXSDXXXHKX XXXXSFXDXX XLXFXXXXXX XXXXXXXXXX XXXXXXXPPX PXKXXXKXXX XXRARXXAVX PXXXXFXXXX XXXXXXGXLX XXXXPXXLXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
tggnnnnnnn nnnaccgtgg acagactagt cgctgctgtc cgtagtggtg atcgcgtggc attgccgcgg gctataacgt tggtggagtc cactcgctcc gaccatcgtg agcaggcgca gcagctgctg ctggcattgc aaccggatgc tggaaacgcc catcgggtgg gcatcaccgg ggtgcccggg gtaggaaagt ccaccgccat tgaggcgctt ggcatgcatc tgatcgaacg gggtcatcga gtggcggtgc tggccgtcga cccatcatcg gtccgtaccg gtggatccat ccttggcgac aagacccgga tggcgcggct agcgnnacac ccggacgcct atatccggcc atcgccgaca tnnnnnacgt tggnnnnngt gncnnnngnn actcnnnaaa canttgtcnt gttggaggcn gntggnttng atgnaattct gatcnnnnnt gtangcgncg gatantctan nnnnggataa natgnncgac accttcnntt ngnnggnnnn gnnncgcnnn nanatcagnn gnatgnnatn aannnnngng ngnnnnnnnt nnnnnacnnc nngnnggtna annnggccga ngnnnagnnn nngnnntntn tnncnncncn nnanctgttt tnnncnctcc gattgnnntn tacacaaaan tttnnanncn naaagttttc nngattnnnn gngactgann tttnnnnnnn ancnanannt nnnanncncn cnnngnnatc nncannnnnt tngcnnngnn ngnnnttnnn nnccnccnnn nccncanaag nnnnnnnaaa aannnnngnc cgngnnncgn gctagancng nngctgtcnn ccccnnggng gnganattcn ngntnagntg nnntgncnng ngnnnancag ggnanctgtn ccnnnntgnn ngnccnnnnn nnctgnagna gnnnnnnnnt cannaanaan ttnnnnannn annttntnnn gnncnnntng nnannnnnnc ccnaannnnn nnnnnnnnnn nnnnnnnnnc nnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGDSTVDR LVAAVRSGDR VALPRAITLV ESTRSDHREQ AQQLLLALQP DAGNAHRVGI TGVPGVGKST AIEALGMHLI ERGHRVAVLA VDPSSVRTGG SILGDKTRMA RLAVHPDAYI RPSPTSGTLG GVTKATRETV VLLEAAGFDV ILIETVGVGQ SEVAVANMVD TFVLLALART GDQLQGIKKG VLELADIVVV NKADGEHLRD ARLAARDLSS ALRLIYTHEK IWRPKVFTIS ATEGTGLTQM WEAVECHRQV LTASGEFENR RLAQQVEWAW QMVRDAVLDR VLSNPAVREI RAELECQVRA GELTPAMAAQ QILQAASC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgggggac agcaccgtgg acagactagt cgctgctgtc cgtagtggtg atcgcgtggc attgccgcgg gctataacgt tggtggagtc cactcgctcc gaccatcgtg agcaggcgca gcagctgctg ctggcattgc aaccggatgc tggaaacgcc catcgggtgg gcatcaccgg ggtgcccggg gtaggaaagt ccaccgccat tgaggcgctt ggcatgcatc tgatcgaacg gggtcatcga gtggcggtgc tggccgtcga cccatcatcg gtccgtaccg gtggatccat ccttggcgac aagacccgga tggcgcggct agcggtacac ccggacgcct atatccggcc atcgccgaca tcgggcacgt tgggcggggt gacgaaggcc actcgggaaa cagttgtcct gttggaggcg gctggttttg atgtaattct gatcgaaact gtaggcgtcg gacagtctga ggtagcggtg gctaatatgg tcgacacctt cgttttgttg gccttggcgc gtaccggcga tcagttgcag ggcatcaaaa aaggcgtgct ggagctggcc gacatcgtgg tggtgaacaa ggccgatggg gagcatctgc gcgatgctcg gctagctgct cgggacctgt catcggcact ccgattgatc tatacacacg aaaaaatttg gagaccaaaa gttttcacga ttagtgcgac tgaggggacc ggtctgaccc agatgtggga agcggtcgaa tgtcatcgtc aagtgcttac tgcctccggc gagtttgaga accgtcgact ggcccagcaa gtcgagtggg cttggcagat ggtccgtgac gcggtgctag accgggtgct gtccaacccc gcggtgcgtg agattcgtgc tgagctggag tgtcaggtgc gagcagggga gctgactccc gcgatggcgg cccagcaaat cctgcaggca gcatcctgca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga 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