MythA.00200.a
LAO/AO transport system ATPase (A0QX37 homolog)
| CENTER ID: | MythA.00200.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00200.a.A1.GE28715 | Full length( MythA.00200.a ) | 1 | 337 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00200.a.A1.PS00590 | Full length( MythA.00200.a ) | 1 | 337 |
Structures
3TK1
DEPOSITED: 8/25/2011
DETERMINATION: XRay
CLONE: MythA.00200.a.A1.GE28715
PROTEIN: MythA.00200.a.A1.PS00590
Publications by SSGCID
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Abendroth J, Baugh L, Baydo RO, Bullen J, Clifton MC, Edwards TE, Grundner C, Lorimer DD, Myler PJ, Phan IQ, Sankaran B, Staker BL, Thompkins K, Van Voorhis WC, Witte P
J. Struct. Funct. Genomics - 2015
volume 16, issue 2, pages 91-9
PMID: 25832174; PMCID: PMC4631608
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.55 |
| RefSeq: | WP_003923457.1 |
| UniProt: | G7CAR0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQTVKDLAA AIRSGDRAAL PRAITLVEST RADHRERAQE LLLELMPDAG RAMHVGITGV PGVGKSTSIE ALGMHLIEQG HRVAVLAVDP SSVRSGGSIL GDKTRMARLA VHPDAYVRPS PSSGTLGGVA KATRETIVLL EAAGFDVVLV ETVGVGQSEV TVADMVDTFV FLTLARTGDQ LQGIKKGVLE LADIVVVNKA DGEHAVEARK AARELAGAMR LIYPRETLWR PPVLTMSALE GTGVAELWET VLKHRQVLID AGEFEKRRRD QEVNWMWTMV RDAVIDRVMT SPGVREIRAE VERQVREGEL TAALAAQRLL DAAELR
NT Sequence
ATGACCCAGA CCGTCAAAGA TCTCGCCGCC GCGATCCGCA GCGGTGATCG CGCCGCCCTG CCCAGGGCGA TCACCCTGGT CGAATCCACC CGCGCCGATC ACCGCGAACG CGCGCAGGAA CTGCTGCTGG AGTTGATGCC GGACGCCGGC AGGGCCATGC ACGTCGGCAT CACCGGGGTG CCCGGGGTGG GTAAGTCGAC CAGCATCGAG GCGCTGGGCA TGCATCTGAT CGAACAGGGC CACAGGGTCG CGGTGCTGGC GGTGGACCCG TCGTCGGTGC GCAGCGGCGG TTCGATCCTC GGCGACAAGA CCCGGATGGC GCGGCTGGCG GTGCATCCCG ACGCCTACGT CCGTCCGTCG CCGTCGTCGG GCACCCTCGG CGGGGTGGCC AAGGCCACCC GGGAGACAAT CGTGCTGCTC GAGGCCGCCG GCTTCGATGT GGTGCTGGTC GAGACGGTCG GGGTGGGTCA ATCCGAGGTG ACCGTGGCCG ACATGGTCGA CACCTTCGTG TTCCTGACCC TGGCCCGCAC CGGGGATCAG CTGCAGGGCA TCAAGAAGGG GGTGCTCGAG CTCGCCGACA TCGTGGTGGT CAACAAGGCC GACGGCGAGC ACGCGGTGGA GGCCCGCAAG GCGGCCCGCG AGCTCGCCGG CGCGATGCGG CTGATCTATC CGCGCGAGAC GCTGTGGCGG CCGCCGGTGC TCACCATGAG CGCGCTGGAG GGCACCGGGG TGGCCGAGCT CTGGGAGACG GTGCTCAAAC ACCGGCAGGT GCTCATCGAC GCGGGGGAGT TCGAGAAGCG CCGGCGCGAC CAAGAGGTCA ACTGGATGTG GACGATGGTC CGGGACGCCG TGATCGACCG GGTGATGACC AGTCCGGGGG TCCGCGAGAT CCGGGCCGAG GTGGAGCGTC AGGTCCGGGA GGGCGAGCTG ACCGCGGCAC TTGCTGCCCA GCGTCTGCTG GACGCCGCCG AGCTGCGTTA A
Details for MythA.00200.a.A1.GE28715
| HARVESTED ON: | 3/1/2010 |
| SEQUENCED ON: | 3/5/2010 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTVKDLA AAIRSGDRAA LPRAITLVES TRADHRERAQ ELLLELMPDA GRAMHVGITG VPGVGKSTSI EALGMHLIEQ GHRVAVLAVD PSSVRSGGSI LGDKTRMARL AVHPDAYVRP SPSSGTLGGV AKATRETIVL LEAAGFDVVL VETVGVGQSE VTVADMVDTF VFLTLARTGD QLQGIKKGVL ELADIVVVNK ADGEHAVEAR KAARELAGAM RLIYPRETLW RPPVLTMSAL EGTGVAELWE TVLKHRQVLI DAGEFEKRRR DQEVNWMWTM VRDAVIDRVM TSPGVREIRA EVERQVREGE LTAALAAQRL LDAAELR
Validated NT Sequence
ttattaacgc agctcggcgg cgtccagcag acgctgggca gcaagtgccg cggtcagctc gccctcccgg acctgacgct ccacctcggc ccggatctcg cggacccccg gactggtcat cacccggtcg atcacggcgt cccggaccat cgtccacatc cagttgacct cttggtcgcg ccggcgcttc tcgaactccc ccgcgtcgat gagcacctgc cggtgtttga gcaccgtctc ccagagctcg gccaccccgg tgccctccag cgcgctcatg gtgagcaccg gcggccgcca cagcgtctcg cgcggataga tcagccgcat cgcgccggcg agctcgcggg ccgccttgcg ggcctccacc gcgtgctcgc cgtcggcctt gttgaccacc acgatgtcgg cgagctcgag cacccccttc ttgatgccct gcagctgatc cccggtgcgg gccagggtca ggaacacgaa ggtgtcgacc atgtcggcca cggtcacctc ggattgaccc accccgaccg tctcgaccag caccacatcg aagccggcgg cctcgagcag cacgattgtc tcccgggtgg ccttggccac cccgccgagg gtgcccgacg acggcgacgg acggacgtag gcgtcgggat gcaccgccag ccgcgccatc cgggtcttgt cgccgaggat cgaaccgccg ctgcgcaccg acgacgggtc caccgccagc accgcgaccc tgtggccctg ttcgatcaga tgcatgccca gcgcctcgat gctggtcgac ttacccaccc cgggcacccc ggtgatgccg acgtgcatgg ccctgccggc gtccggcatc aactccagca gcagttcctg cgcgcgttcg cggtgatcgg cgcgggtgga ttcgaccagg gtgatcgccc tgggcagggc ggcgcgatca ccgctgcgga tcgcggcggc gagatctttg acggtctggg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTVKDLA AAIRSGDRAA LPRAITLVES TRADHRERAQ ELLLELMPDA GRAMHVGITG VPGVGKSTSI EALGMHLIEQ GHRVAVLAVD PSXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXSPSSGT LGGVAKATRE TIVLLEAAGF DVVLVETVGV GQSEVTVADM VDTFVFLTLA RTGDQLQGIK KGVLELADIV VVNKADGEHA VEARKAAREL AGAMRLIYXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXL IDAGEFEKRR RDQEVNWMWT MVRDAVIDRV MTSPGVREIR AEVERQVREG ELTAALAAQR LLDAAELR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccagacc gtcaaagatc tcgccgccgc gatccgcagc ggtgatcgcg ccgccctgcc cagggcgatc accctggtcg aatccacccg cgccgatcac cgcgaacgcg cgcaggaact gctgctggag ttgatgccgg acgccggcag ggccatgcac gtcggcatca ccggggtgcc cggggtgggt aagtcgacca gcatcgaggc gctgggcatg catctgatcg aacagggcca cagggtcgcg gtgctggcgg tggacccgtc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcg ccgtcgtcgg gcaccctcgg cggggtggcc aaggccaccc gggagacaat cgtgctgctc gaggccgccg gcttcgatgt ggtgctggtc gagacggtcg gggtgggtca atccgaggtg accgtggccg acatggtcga caccttcgtg ttcctgaccc tggcccgcac cggggatcag ctgcagggca tcaagaaggg ggtgctcgag ctcgccgaca tcgtggtggt caacaaggcc gacggcgagc acgcggtgga ggcccgcaag gcggcccgcg agctcgccgg cgcgatgcgg ctgatctatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctcatcga cgcgggggag ttcgagaagc gccggcgcga ccaagaggtc aactggatgt ggacgatggt ccgggacgcc gtgatcgacc gggtgatgac cagtccgggg gtccgcgaga tccgggccga ggtggagcgt caggtccggg agggcgagct gaccgcggca cttgctgccc agcgtctgct ggacgccgcc gagctgcgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MythA.00200.a.A1.PS00590
| PURIFICATION DATe: | 4/30/2010 |
| CONCENTRATION: | 42.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTVKDLA AAIRSGDRAA LPRAITLVES TRADHRERAQ ELLLELMPDA GRAMHVGITG VPGVGKSTSI EALGMHLIEQ GHRVAVLAVD PSSVRSGGSI LGDKTRMARL AVHPDAYVRP SPSSGTLGGV AKATRETIVL LEAAGFDVVL VETVGVGQSE VTVADMVDTF VFLTLARTGD QLQGIKKGVL ELADIVVVNK ADGEHAVEAR KAARELAGAM RLIYPRETLW RPPVLTMSAL EGTGVAELWE TVLKHRQVLI DAGEFEKRRR DQEVNWMWTM VRDAVIDRVM TSPGVREIRA EVERQVREGE LTAALAAQRL LDAAELR
Validated NT Sequence
ttattaacgc agctcggcgg cgtccagcag acgctgggca gcaagtgccg cggtcagctc gccctcccgg acctgacgct ccacctcggc ccggatctcg cggacccccg gactggtcat cacccggtcg atcacggcgt cccggaccat cgtccacatc cagttgacct cttggtcgcg ccggcgcttc tcgaactccc ccgcgtcgat gagcacctgc cggtgtttga gcaccgtctc ccagagctcg gccaccccgg tgccctccag cgcgctcatg gtgagcaccg gcggccgcca cagcgtctcg cgcggataga tcagccgcat cgcgccggcg agctcgcggg ccgccttgcg ggcctccacc gcgtgctcgc cgtcggcctt gttgaccacc acgatgtcgg cgagctcgag cacccccttc ttgatgccct gcagctgatc cccggtgcgg gccagggtca ggaacacgaa ggtgtcgacc atgtcggcca cggtcacctc ggattgaccc accccgaccg tctcgaccag caccacatcg aagccggcgg cctcgagcag cacgattgtc tcccgggtgg ccttggccac cccgccgagg gtgcccgacg acggcgacgg acggacgtag gcgtcgggat gcaccgccag ccgcgccatc cgggtcttgt cgccgaggat cgaaccgccg ctgcgcaccg acgacgggtc caccgccagc accgcgaccc tgtggccctg ttcgatcaga tgcatgccca gcgcctcgat gctggtcgac ttacccaccc cgggcacccc ggtgatgccg acgtgcatgg ccctgccggc gtccggcatc aactccagca gcagttcctg cgcgcgttcg cggtgatcgg cgcgggtgga ttcgaccagg gtgatcgccc tgggcagggc ggcgcgatca ccgctgcgga tcgcggcggc gagatctttg acggtctggg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTVKDLA AAIRSGDRAA LPRAITLVES TRADHRERAQ ELLLELMPDA GRAMHVGITG VPGVGKSTSI EALGMHLIEQ GHRVAVLAVD PSXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXSPSSGT LGGVAKATRE TIVLLEAAGF DVVLVETVGV GQSEVTVADM VDTFVFLTLA RTGDQLQGIK KGVLELADIV VVNKADGEHA VEARKAAREL AGAMRLIYXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXL IDAGEFEKRR RDQEVNWMWT MVRDAVIDRV MTSPGVREIR AEVERQVREG ELTAALAAQR LLDAAELR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCAGACC GTCAAAGATC TCGCCGCCGC GATCCGCAGC GGTGATCGCG CCGCCCTGCC CAGGGCGATC ACCCTGGTCG AATCCACCCG CGCCGATCAC CGCGAACGCG CGCAGGAACT GCTGCTGGAG TTGATGCCGG ACGCCGGCAG GGCCATGCAC GTCGGCATCA CCGGGGTGCC CGGGGTGGGT AAGTCGACCA GCATCGAGGC GCTGGGCATG CATCTGATCG AACAGGGCCA CAGGGTCGCG GTGCTGGCGG TGGACCCGTC GTNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGTCG CCGTCGTCGG GCACCCTCGG CGGGGTGGCC AAGGCCACCC GGGAGACAAT CGTGCTGCTC GAGGCCGCCG GCTTCGATGT GGTGCTGGTC GAGACGGTCG GGGTGGGTCA ATCCGAGGTG ACCGTGGCCG ACATGGTCGA CACCTTCGTG TTCCTGACCC TGGCCCGCAC CGGGGATCAG CTGCAGGGCA TCAAGAAGGG GGTGCTCGAG CTCGCCGACA TCGTGGTGGT CAACAAGGCC GACGGCGAGC ACGCGGTGGA GGCCCGCAAG GCGGCCCGCG AGCTCGCCGG CGCGATGCGG CTGATCTATC CNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TGCTCATCGA CGCGGGGGAG TTCGAGAAGC GCCGGCGCGA CCAAGAGGTC AACTGGATGT GGACGATGGT CCGGGACGCC GTGATCGACC GGGTGATGAC CAGTCCGGGG GTCCGCGAGA TCCGGGCCGA GGTGGAGCGT CAGGTCCGGG AGGGCGAGCT GACCGCGGCA CTTGCTGCCC AGCGTCTGCT GGACGCCGCC GAGCTGCGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A