MyulA.00200.a

Transport system kinase

CENTER ID: MyulA.00200.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00200.a.A1.GE29659 Full length( MyulA.00200.a ) 1 292
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_131892
PATRIC ID: fig|362242.7.peg.491
RefSeq: YP_904549.1
UniProt: A0PL59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIDDLIAAA RNGSQRAAGR LLSLIEGDRR DEVLAGIEDW SQRPARVIGI TGPPGAGKST TITGLVGAYR ERGTRVAVLA VDPSSPFSGG ALLGDRIRMT AHVNDSEVLI RSVASRGHLG GLAASVPAAI RLLGALRYDV VLLKTVGVGQ SEIEVVAVAD PTVVVLNPGT GDAVQAAKAG LLEVADIVAV NKADRVGAEQ TVRDLRAETN APILSLVAVK SEGIGELLAL IDAHQRGDSR DRRLARARAQ ILSLAQTRLR GRTDLDRLAE AVVDGLEDPY AAADRLLAPP PP
NT Sequence
ATGAGCATCG ATGATCTGAT TGCCGCTGCG CGCAACGGAT CTCAACGCGC CGCGGGCCGG CTCCTCAGTC TGATCGAGGG GGACCGCCGC GATGAAGTGC TGGCGGGTAT CGAGGACTGG TCGCAGCGGC CCGCGCGAGT CATCGGCATC ACCGGGCCGC CAGGGGCGGG CAAATCGACG ACGATCACCG GGCTGGTCGG CGCCTACCGG GAGCGCGGAA CCCGGGTGGC GGTGCTAGCC GTGGACCCGT CGTCCCCGTT CAGTGGTGGT GCGTTGCTCG GCGATCGCAT CCGAATGACC GCGCATGTCA ACGACTCCGA GGTGCTGATC CGCTCGGTGG CCAGTCGGGG CCATCTTGGC GGTCTGGCCG CCTCGGTGCC CGCCGCCATC CGGCTGCTGG GGGCGCTGCG TTACGACGTC GTTCTGCTGA AAACGGTCGG GGTGGGACAG TCCGAGATCG AGGTGGTGGC GGTGGCAGAC CCGACCGTCG TCGTGCTCAA TCCGGGAACC GGCGATGCGG TCCAGGCGGC AAAGGCGGGT CTGCTGGAAG TCGCCGACAT TGTGGCGGTC AACAAGGCCG ACCGCGTCGG TGCCGAACAG ACCGTGCGAG ACTTGCGGGC CGAAACCAAT GCCCCGATCC TCAGCCTGGT CGCGGTCAAG TCGGAGGGCA TTGGTGAGCT GTTGGCCCTG ATCGACGCGC ATCAGCGCGG CGATAGTCGC GATCGCCGGT TGGCCCGCGC CCGGGCACAG ATTCTGTCGC TGGCGCAGAC TCGGCTGCGC GGCCGTACCG ACCTGGACCG GCTCGCCGAA GCGGTGGTCG ACGGCCTCGA GGATCCTTAT GCTGCGGCGG ACCGGCTGTT GGCCCCGCCG CCGCCC
Details for MyulA.00200.a.A1.GE29659
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 32kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 68
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIDDLIAA ARNGSQRAAG RLLSLIEGDR RDEVLAGIED WSQRPARVIG ITGPPGAGKS TTITGLVGAY RERGTRVAVL AVDPSSPFSG GALLGDRIRM TAHVNDSEVL IRSVASRGHL GGLAASVPAA IRLLGALRYD VVLLKTVGVG QSEIEVVAVA XPTVVVLNPG TGXAVXAAKG VCWXSPXLXA XTXXXSVPNX XXXXXXXXXX PXLXXXXXXX XXXXXXXXXX XXGXXRXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
tttntttanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcat cgatgatctg attgccgctg cgcgcaacgg atctcaacgc gccgcgggcc ggctcctcag tctgatcgag ggggaccgcc gcgatgaagt gctggcgggt atcgaggact ggtcgcagcg gcccgcgcga gtcatcggca tcaccgggcc gccaggggcg ggcaaatcga cgacgatcac cgggctggtc ggcgcctacc gggagcgcgg aacccgggtg gcggtgctag ccgtggaccc gtcgtccccg ttcagtggtg gtgcgttgct cggcgatcgc atccgaatga ccgcgcatgt caacgactcc gaggtgctga tccgctcggt ggccagtcgg ggccatcttg gcggtctggc cgcctcggtg cccgccgcca tccggctgct gggggcgctg cgttacgacg tcgttctgct gaaaacggtc ggggtgggac agtccgagat cgaggtggtg gcggtggcan anccgaccgt cgtcgtgctc aatccgggaa ccggcnatgc ngtccnggcg gcaaaggggg tctgctggna gtcgccnann ttgnnngcgg ncacnnncna cnnntcggtg ccnaacnnnn cgnnnnanan ttgnnnggnn nannnnngcc cnnanctcan nnnnnnngnn annnnnnnnn nngnnnnnnt nnnnnnnnna nnnannnnnn nnnnnggnnn nntncgnnnn cnnnnnnnnn nnnnnnntnn nnannnnnnn nnnnncnncn gncnnnnnnn nngnnnnann nnnnnnntnn nnnnnnnnnn nnnnnnnnnn nnnnnannnn nnnnnnnnnn nnnaaannan a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIDDLIAA ARNGSQRAAG RLLSLIEGDR RDEVLAGIED WSQRPARVIG ITGPPGAGKS TTITGLVGAY RERGTRVAVL AVDPSSPFSG GALLGDRIRM TAHVNDSEVL IRSVASRGHL GGLAASVPAA IRLLGALRYD VVLLKTVGVG QSEIEVVAVA DPTVVVLNPG TGDAVQAAKA GLLEVADIVA VNKADRVGAE QTVRDLRAET NAPILSLVAV KSEGIGELLA LIDAHQRGDS RDRRLARARA QILSLAQTRL RGRTDLDRLA EAVVDGLEDP YAAADRLLAP PPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcatcgat gatctgattg ccgctgcgcg caacggatct caacgcgccg cgggccggct cctcagtctg atcgaggggg accgccgcga tgaagtgctg gcgggtatcg aggactggtc gcagcggccc gcgcgagtca tcggcatcac cgggccgcca ggggcgggca aatcgacgac gatcaccggg ctggtcggcg cctaccggga gcgcggaacc cgggtggcgg tgctagccgt ggacccgtcg tccccgttca gtggtggtgc gttgctcggc gatcgcatcc gaatgaccgc gcatgtcaac gactccgagg tgctgatccg ctcggtggcc agtcggggcc atcttggcgg tctggccgcc tcggtgcccg ccgccatccg gctgctgggg gcgctgcgtt acgacgtcgt tctgctgaaa acggtcgggg tgggacagtc cgagatcgag gtggtggcgg tggcagaccc gaccgtcgtc gtgctcaatc cgggaaccgg cgatgcggtc caggcggcaa aggcgggtct gctggaagtc gccgacattg tggcggtcaa caaggccgac cgcgtcggtg ccgaacagac cgtgcgagac ttgcgggccg aaaccaatgc cccgatcctc agcctggtcg cggtcaagtc ggagggcatt ggtgagctgt tggccctgat cgacgcgcat cagcgcggcg atagtcgcga tcgccggttg gcccgcgccc gggcacagat tctgtcgctg gcgcagactc ggctgcgcgg ccgtaccgac ctggaccggc tcgccgaagc ggtggtcgac ggcctcgagg atccttatgc tgcggcggac cggctgttgg ccccgccgcc gcccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa