MysmA.00200.a
LAO/AO transport system ATPase
| CENTER ID: | MysmA.00200.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00200.a.A1.GE28581 | Full length( MysmA.00200.a ) | 1 | 294 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00200.a.A1.PS00535 | Full length( MysmA.00200.a ) | 1 | 294 |
Structures
6CUM
DEPOSITED: 3/26/2018
DETERMINATION: XRay
CLONE: MysmA.00200.a.A1.GE28581
PROTEIN: MysmA.00200.a.A1.PS00535
Publications by SSGCID
Ab initio structure solution of a proteolytic fragment using ARCIMBOLDO
Abendroth J, Edwards TE, Lorimer DD, Myler PJ, Sankaran B
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2018
volume F74, pages 530-535
PMID: 30198884; PMCID: PMC6130419
PDB:
6CUM
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131892 |
| PATRIC ID: | fig|246196.19.peg.4752 |
| RefSeq: | YP_889126.1 |
| UniProt: | A0R1T8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPHGVVDVPE LITVARGGSM RAVGRLLTLV ESDRRGEVLA ALGPATPRVI GVTGPPGAGK STTVGAMVGA YRERGLRVAV LAVDPSSPYS GGALLGDRIR MAAHINDPDV LIRSMAARGH LGGLAAAVPA AIRLLAALSY DLIVLETVGV GQSEIEIAAI ADPTVVILNP GAGDAVQAAK AGVLEVADLV VVNKADRDGA DQTVRDLRAE TDVPVLKLVA AQGDGLHELI EAIEAHQRAD TPERRRARAR SQILSLAQTL LRNHADLDRL SAAVADGSSD AYTAAERLFA GSVD
NT Sequence
ATGCCCCACG GCGTCGTCGA TGTACCCGAG CTCATCACCG TGGCCAGGGG CGGATCGATG CGCGCGGTGG GTCGGCTGCT CACGCTGGTC GAAAGCGACC GTCGCGGTGA GGTGCTCGCG GCGCTCGGGC CCGCGACGCC GCGCGTCATC GGTGTGACCG GACCGCCGGG AGCGGGCAAG TCGACCACGG TGGGCGCCAT GGTGGGGGCC TATCGCGAAC GCGGGCTGCG GGTCGCGGTC CTCGCGGTCG ACCCCTCGTC GCCCTACAGC GGCGGGGCAC TGCTCGGTGA CCGCATCCGC ATGGCCGCCC ACATCAACGA CCCCGACGTG CTGATCCGGT CGATGGCCGC ACGCGGGCAC CTCGGCGGGC TGGCCGCGGC CGTCCCGGCG GCGATCCGCC TGCTGGCCGC GCTGTCGTAC GACCTGATCG TGCTGGAGAC CGTGGGGGTG GGACAGTCCG AGATCGAGAT CGCCGCGATC GCCGACCCCA CGGTGGTGAT CCTCAACCCC GGCGCGGGTG ATGCCGTGCA GGCCGCCAAG GCCGGTGTGC TCGAGGTGGC CGATCTGGTG GTGGTCAACA AGGCCGATCG TGACGGCGCC GACCAGACGG TCCGCGATCT GCGCGCCGAA ACCGACGTCC CAGTGCTCAA ACTCGTCGCC GCGCAGGGGG ACGGCCTGCA CGAACTCATC GAGGCCATCG AGGCGCACCA GCGCGCCGAC ACCCCCGAAC GGCGCCGCGC GCGGGCGCGC AGCCAGATCC TGTCGCTCGC GCAGACCCTG CTGCGCAACC ACGCCGACCT CGACCGGTTG TCGGCGGCCG TGGCCGACGG CAGCAGCGAC GCCTACACCG CGGCCGAGCG GCTGTTCGCC GGATCAGTCG AC
Details for MysmA.00200.a.A1.GE28581
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPHGVVDVP ELITVARGGS MRAVGRLLTL VESDRRGEVL AALGPATPRV IGVTGPPGAG KSTTVGAMVG AYRERGLRVA VLAVDPSSPY SGGALLGDRI RMAAHINDPD VLIRSMAARG HLGGLAAAVP AAIRLLAALS YDLIVLETVG VGQSEIEIAA IADPTVVILN PGAGDAVQAA KAGVLEVADL VVVNKADRDG ADQTVRDLRA ETDVPVLKLV AAQGDGLHEL IEAIEAHQRA DTPERRRARA RSQILSLAQT LLRNHADLDR LSAAVADGSS DAYTAAERLF AGSVD
Validated NT Sequence
gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg ccccacggcg tcgtcgatgt acccgagctc atcaccgtgg ccaggggcgg atcgatgcgc gcggtgggtc ggctgctcac gctggtcgaa agcgaccgtc gcggtgaggt gctcgcggcg ctcgggcccg cgacgccgcg cgtcatcggt gtgaccggac cgccgggagc gggcaagtcg accacggtgg gcgccatggt gggggcctat cgcgaacgcg ggctgcgggt cgcggtcctc gcggtcgacc cctcgtcgcc ctacagcggc ggggcactgc tcggtgaccg catccgcatg gccgcccaca tcaacgaccc cgacgtgctg atccggtcga tggccgcacg cgggcacctc ggcgggctgg ccgcggccgt cccggcggcg atccgcctgc tggccgcgct gtcgtacgac ctgatcgtgc tggagaccgt gggggtggga cagtccgaga tcgagatcgc cgcgatcgcc gaccccacgg tggtgatcct caaccccggc gcgggtgatg ccgtgcaggc cgccaaggcc ggtgtgctcg aggtggccga tctggtggtg gtcaacaagg ccgatcgtga cggcgccgac cagacggtcc gcgatctgcg cgccgaaacc gacgtcccag tgctcaaact cgtcgccgcg cagggggacg gcctgcacga actcatcgag gccatcgagg cgcaccagcg cgccgacacc cccgaacggc gccgcgcgcg ggcgcgcagc cagatcctgt cgctcgcgca gaccctgctg cgcaaccacg ccgacctcga ccggttgtcg gcggccgtgg ccgacggcag cagcgacgcc tacaccgcgg ccgagcggct gttcgccgga tcagtcgact aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPHGVVDVP ELITVARGGS MRAVGRLLTL VESDRRGEVL AALGPATPRV IGVTGPPGAG KSTTVGAMVG AYRERGLRVA VLAVDPSSPY SGGALLGDRI RMAAHINDPD VLIRSMAARG HLGGLAAAVP AAIRLLAALS YDLIVLETVG VGQSEIEIAA IADPTVVILN PGAGDAVQAA KAGVLEVADL VVVNKADRDG ADQTVRDLRA ETDVPVLKLV AAQGDGLHEL IEAIEAHQRA DTPERRRARA RSQILSLAQT LLRNHADLDR LSAAVADGSS DAYTAAERLF AGSVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccccacggc gtcgtcgatg tacccgagct catcaccgtg gccaggggcg gatcgatgcg cgcggtgggt cggctgctca cgctggtcga aagcgaccgt cgcggtgagg tgctcgcggc gctcgggccc gcgacgccgc gcgtcatcgg tgtgaccgga ccgccgggag cgggcaagtc gaccacggtg ggcgccatgg tgggggccta tcgcgaacgc gggctgcggg tcgcggtcct cgcggtcgac ccctcgtcgc cctacagcgg cggggcactg ctcggtgacc gcatccgcat ggccgcccac atcaacgacc ccgacgtgct gatccggtcg atggccgcac gcgggcacct cggcgggctg gccgcggccg tcccggcggc gatccgcctg ctggccgcgc tgtcgtacga cctgatcgtg ctggagaccg tgggggtggg acagtccgag atcgagatcg ccgcgatcgc cgaccccacg gtggtgatcc tcaaccccgg cgcgggtgat gccgtgcagg ccgccaaggc cggtgtgctc gaggtggccg atctggtggt ggtcaacaag gccgatcgtg acggcgccga ccagacggtc cgcgatctgc gcgccgaaac cgacgtccca gtgctcaaac tcgtcgccgc gcagggggac ggcctgcacg aactcatcga ggccatcgag gcgcaccagc gcgccgacac ccccgaacgg cgccgcgcgc gggcgcgcag ccagatcctg tcgctcgcgc agaccctgct gcgcaaccac gccgacctcg accggttgtc ggcggccgtg gccgacggca gcagcgacgc ctacaccgcg gccgagcggc tgttcgccgg atcagtcgac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.00200.a.A1.PS00535
| PURIFICATION DATe: | 3/24/2010 |
| CONCENTRATION: | 60.56mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPHGVVDVP ELITVARGGS MRAVGRLLTL VESDRRGEVL AALGPATPRV IGVTGPPGAG KSTTVGAMVG AYRERGLRVA VLAVDPSSPY SGGALLGDRI RMAAHINDPD VLIRSMAARG HLGGLAAAVP AAIRLLAALS YDLIVLETVG VGQSEIEIAA IADPTVVILN PGAGDAVQAA KAGVLEVADL VVVNKADRDG ADQTVRDLRA ETDVPVLKLV AAQGDGLHEL IEAIEAHQRA DTPERRRARA RSQILSLAQT LLRNHADLDR LSAAVADGSS DAYTAAERLF AGSVD
Validated NT Sequence
gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg ccccacggcg tcgtcgatgt acccgagctc atcaccgtgg ccaggggcgg atcgatgcgc gcggtgggtc ggctgctcac gctggtcgaa agcgaccgtc gcggtgaggt gctcgcggcg ctcgggcccg cgacgccgcg cgtcatcggt gtgaccggac cgccgggagc gggcaagtcg accacggtgg gcgccatggt gggggcctat cgcgaacgcg ggctgcgggt cgcggtcctc gcggtcgacc cctcgtcgcc ctacagcggc ggggcactgc tcggtgaccg catccgcatg gccgcccaca tcaacgaccc cgacgtgctg atccggtcga tggccgcacg cgggcacctc ggcgggctgg ccgcggccgt cccggcggcg atccgcctgc tggccgcgct gtcgtacgac ctgatcgtgc tggagaccgt gggggtggga cagtccgaga tcgagatcgc cgcgatcgcc gaccccacgg tggtgatcct caaccccggc gcgggtgatg ccgtgcaggc cgccaaggcc ggtgtgctcg aggtggccga tctggtggtg gtcaacaagg ccgatcgtga cggcgccgac cagacggtcc gcgatctgcg cgccgaaacc gacgtcccag tgctcaaact cgtcgccgcg cagggggacg gcctgcacga actcatcgag gccatcgagg cgcaccagcg cgccgacacc cccgaacggc gccgcgcgcg ggcgcgcagc cagatcctgt cgctcgcgca gaccctgctg cgcaaccacg ccgacctcga ccggttgtcg gcggccgtgg ccgacggcag cagcgacgcc tacaccgcgg ccgagcggct gttcgccgga tcagtcgact aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPHGVVDVP ELITVARGGS MRAVGRLLTL VESDRRGEVL AALGPATPRV IGVTGPPGAG KSTTVGAMVG AYRERGLRVA VLAVDPSSPY SGGALLGDRI RMAAHINDPD VLIRSMAARG HLGGLAAAVP AAIRLLAALS YDLIVLETVG VGQSEIEIAA IADPTVVILN PGAGDAVQAA KAGVLEVADL VVVNKADRDG ADQTVRDLRA ETDVPVLKLV AAQGDGLHEL IEAIEAHQRA DTPERRRARA RSQILSLAQT LLRNHADLDR LSAAVADGSS DAYTAAERLF AGSVD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCCACGGC GTCGTCGATG TACCCGAGCT CATCACCGTG GCCAGGGGCG GATCGATGCG CGCGGTGGGT CGGCTGCTCA CGCTGGTCGA AAGCGACCGT CGCGGTGAGG TGCTCGCGGC GCTCGGGCCC GCGACGCCGC GCGTCATCGG TGTGACCGGA CCGCCGGGAG CGGGCAAGTC GACCACGGTG GGCGCCATGG TGGGGGCCTA TCGCGAACGC GGGCTGCGGG TCGCGGTCCT CGCGGTCGAC CCCTCGTCGC CCTACAGCGG CGGGGCACTG CTCGGTGACC GCATCCGCAT GGCCGCCCAC ATCAACGACC CCGACGTGCT GATCCGGTCG ATGGCCGCAC GCGGGCACCT CGGCGGGCTG GCCGCGGCCG TCCCGGCGGC GATCCGCCTG CTGGCCGCGC TGTCGTACGA CCTGATCGTG CTGGAGACCG TGGGGGTGGG ACAGTCCGAG ATCGAGATCG CCGCGATCGC CGACCCCACG GTGGTGATCC TCAACCCCGG CGCGGGTGAT GCCGTGCAGG CCGCCAAGGC CGGTGTGCTC GAGGTGGCCG ATCTGGTGGT GGTCAACAAG GCCGATCGTG ACGGCGCCGA CCAGACGGTC CGCGATCTGC GCGCCGAAAC CGACGTCCCA GTGCTCAAAC TCGTCGCCGC GCAGGGGGAC GGCCTGCACG AACTCATCGA GGCCATCGAG GCGCACCAGC GCGCCGACAC CCCCGAACGG CGCCGCGCGC GGGCGCGCAG CCAGATCCTG TCGCTCGCGC AGACCCTGCT GCGCAACCAC GCCGACCTCG ACCGGTTGTC GGCGGCCGTG GCCGACGGCA GCAGCGACGC CTACACCGCG GCCGAGCGGC TGTTCGCCGG ATCAGTCGAC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA