SSGCID: Target Page

MypaA.00200.a

Putative uncharacterized protein

CENTER ID:	MypaA.00200.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00200.a.A1.GE29187	Full length( MypaA.00200.a )		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131892
PATRIC ID:	fig\|262316.17.peg.1292
RefSeq:	NP_960161.1
UniProt:	Q740W5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGRTVTELA AAIRSGDRAA LPRAITLLES TRADHQEQAQ QLLLELTPDA GDAHRVGITG IPGVGKSTSI EALGMHLIGL GHKVAVLAVD PSSTRTGGSI LGDKTRMARL ANHPDAYIRP SPTSGTLGGV AKATRETVVL LEATGFDVIL IETVGVGQSE VTVANMVDTF VLLTLARGGD QLQGIKKGVL ELADIVVVNK ADGEHLAEAR SAARELSGAI RLIHPRETLW RPPVLTMSAA QGTGLPELWE TIERHRQVLS KAGEFEARRR SQLVDWTWQM VRDTVLDRLL SNPAVRTMRA DVERRVKAGE LTPALAAQEI LAAASR

NT Sequence

ATGAGCGGGC GGACGGTGAC CGAGCTCGCC GCGGCGATCC GCAGCGGTGA CCGCGCGGCG CTGCCGCGCG CCATCACGCT GTTGGAGTCC ACCCGCGCCG ACCATCAGGA GCAGGCCCAG CAGCTGTTGT TGGAGCTGAC GCCGGATGCC GGCGACGCGC ATCGAGTCGG CATCACCGGC ATCCCCGGGG TGGGCAAGTC CACCAGCATC GAGGCGCTCG GCATGCACCT GATCGGGCTC GGCCACAAGG TGGCGGTGCT GGCCGTCGAC CCGTCGTCGA CCCGCACCGG TGGCTCCATC CTGGGCGACA AGACCCGGAT GGCCCGGCTG GCCAATCATC CGGACGCCTA CATCCGGCCA TCGCCGACGT CGGGCACGCT GGGCGGCGTG GCCAAGGCCA CCCGCGAAAC GGTGGTCCTG CTCGAGGCCA CGGGTTTCGA CGTCATCCTG ATCGAGACCG TCGGCGTCGG CCAGTCCGAG GTGACGGTGG CCAACATGGT CGACACGTTC GTGCTGCTGA CCCTGGCCCG CGGCGGCGAT CAGCTGCAGG GCATCAAGAA GGGCGTGCTG GAGCTGGCCG ACATCGTCGT GGTGAACAAG GCCGACGGCG AGCACCTGGC CGAGGCGCGG TCCGCCGCGC GTGAACTGTC CGGCGCGATC CGGCTGATCC ACCCTCGCGA AACCCTTTGG CGCCCACCGG TTCTCACGAT GAGCGCGGCG CAGGGCACCG GTCTGCCGGA ACTGTGGGAG ACCATCGAAC GGCACCGTCA GGTGCTCAGC AAGGCCGGCG AGTTCGAGGC GCGGCGCCGG TCGCAATTGG TGGACTGGAC CTGGCAGATG GTGCGCGACA CGGTGCTGGA CCGGCTGCTG TCCAACCCGG CGGTCCGCAC GATGCGCGCC GACGTCGAGC GACGCGTCAA GGCGGGGGAG CTCACCCCGG CGCTGGCTGC CCAGGAAATC CTGGCGGCCG CGTCGCGC

Details for MypaA.00200.a.A1.GE29187

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

tagcgcgacg cggccgccag gatttcctgg gcagccagcg ccggggtgag ctcccccgcc ttgacgcgtc gctcgacgtc ggcgcgcatc gtgcggaccg ccgggttgga cagcagccgg tccagcaccg tgtcgcgcac catctgccag gtccagtcca ccaattgcga ccggcgccgc gcctcgaact cgccggcctt gctgagcacc tgacggtgcc gttcgatggt ctcccacagt tccggcagac cggtgccctg cgccgcgctc atcgtgagaa ccggtgggcg ccaaagggtt tcgcgagggt ggatcagccg gatcgcgccg gacagttcac gcgcggcgga ccgcgcctcg gccaggtgct cgccgtcggc cttgttcacc acgacgatgt cggccagctc cagcacgccc ttcttgatgc cctgcagctg atcgccgccg cgggccaggg tcagcagcac gaacgtgtcg accatgttgg ccaccgtcac ctcggactgg ccgacgccga cggtctcgat caggatgacg tcgaaacccg tggcctcgag caggaccacc gtttcgcggg tggccttggc cacgccgccc agcgtgcccg acgtcggcga tggccggatg taggcgtccg gatgattggc cagccgggcc atccgggtct tgtcgcccag gatggagcca ccggtgcggg tcgacgacgg gtcgacggcc agcaccgcca ccttgtggcc gagcccgatc aggtgcatgc cgagcgcctc gatgctggtg gacttgccca ccccggggat gccggtgatg ccgactcgat gcgcgtcgcc ggcatccggc gtcagctcca acaacagctg ctgggcctgc tcctgatggt cggcgcgggt ggactccaac agcgtgatgg cgcgcggcag cgccgcgcgg tcaccgctgc ggatcgccgc ggcgagctcg gtcaccgtcc gcccgctcat cgaaccagga ccctgggtct gagcttcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSGRTVTEL AAAIRSGDRA ALPRAITLLE STRADHQEQA QQLLLELTPD AGDAHRVGIT GIPGVGKSTS IEALGMHLIG LGHKVAVLAV DPSSTRTGGS ILGDKTRMAR LANHPDAYIR PSPTSGTLGG VAKATRETVV LLEATGFDVI LIETVGVGQS EVTVANMVDT FVLLTLARGG DQLQGIKKGV LELADIVVVN KADGEHLAEA RSAARELSGA IRLIHPRETL WRPPVLTMSA AQGTGLPELW ETIERHRQVL SKAGEFEARR RSQLVDWTWQ MVRDTVLDRL LSNPAVRTMR ADVERRVKAG ELTPALAAQE ILAAASR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgggcgg acggtgaccg agctcgccgc ggcgatccgc agcggtgacc gcgcggcgct gccgcgcgcc atcacgctgt tggagtccac ccgcgccgac catcaggagc aggcccagca gctgttgttg gagctgacgc cggatgccgg cgacgcgcat cgagtcggca tcaccggcat ccccggggtg ggcaagtcca ccagcatcga ggcgctcggc atgcacctga tcgggctcgg ccacaaggtg gcggtgctgg ccgtcgaccc gtcgtcgacc cgcaccggtg gctccatcct gggcgacaag acccggatgg cccggctggc caatcatccg gacgcctaca tccggccatc gccgacgtcg ggcacgctgg gcggcgtggc caaggccacc cgcgaaacgg tggtcctgct cgaggccacg ggtttcgacg tcatcctgat cgagaccgtc ggcgtcggcc agtccgaggt gacggtggcc aacatggtcg acacgttcgt gctgctgacc ctggcccgcg gcggcgatca gctgcagggc atcaagaagg gcgtgctgga gctggccgac atcgtcgtgg tgaacaaggc cgacggcgag cacctggccg aggcgcggtc cgccgcgcgt gaactgtccg gcgcgatccg gctgatccac cctcgcgaaa ccctttggcg cccaccggtt ctcacgatga gcgcggcgca gggcaccggt ctgccggaac tgtgggagac catcgaacgg caccgtcagg tgctcagcaa ggccggcgag ttcgaggcgc ggcgccggtc gcaattggtg gactggacct ggcagatggt gcgcgacacg gtgctggacc ggctgctgtc caacccggcg gtccgcacga tgcgcgccga cgtcgagcga cgcgtcaagg cgggggagct caccccggcg ctggctgccc aggaaatcct ggcggccgcg tcgcgcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa