MyulA.00200.b

LAO/AO transport system kinase

CENTER ID: MyulA.00200.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00200.b.A1.GE29660 Full length( MyulA.00200.b ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_131892
PATRIC ID: fig|362242.7.peg.1812
RefSeq: YP_905499.1
UniProt: A0PNV9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSPDDAKVE QLAAAVRNGD RAALPRAITL LESTRADDRR QVQELLLALL PDSGKAHRVG ITGVPGVGKS TTIEALGMYL IEQGHKVAVL AVDPSSTRTG GSILGDKTRM ARLAMHPDAY VRPSPTSGTL GGVAKATRES VVLLEAAGFD VVLVETVGVG QSEVAVANMV DTFVLLTLAR TGDQLQGIKK GVLELADIVV VNKADGEHLR DARLAARELS GAIRLIYPRE TIWRPPVLTM SAVEGTGLTQ LGEAVERHRQ VLTEAGEFEA RRREQQVDWT WQMVRDAVLD RVLSNPDVRK IRADVERRVR AGELTPALAA QQILDTAQS
NT Sequence
GTGACCTCCC CGGACGACGC CAAGGTCGAG CAACTGGCCG CCGCCGTGCG CAATGGCGAC CGTGCCGCGC TGCCACGGGC GATCACGCTG CTGGAGTCCA CCCGCGCCGA CGATCGGCGG CAGGTCCAAG AGCTGTTGCT GGCACTGCTC CCCGACTCCG GGAAGGCCCA TCGGGTGGGT ATTACCGGCG TCCCGGGAGT GGGGAAGTCC ACCACGATCG AGGCCCTGGG CATGTACCTC ATCGAGCAGG GCCACAAGGT GGCCGTGCTG GCGGTGGACC CCTCGTCGAC GCGTACCGGC GGATCGATTC TGGGCGACAA GACGCGGATG GCGCGGTTGG CAATGCACCC GGATGCCTAC GTCCGGCCCT CTCCGACGTC GGGAACTCTG GGCGGCGTAG CAAAGGCAAC GCGGGAATCG GTAGTGCTCT TGGAAGCCGC CGGCTTCGAC GTGGTTCTGG TCGAAACGGT CGGCGTCGGC CAGTCCGAAG TGGCCGTCGC AAACATGGTC GACACCTTCG TCTTGCTGAC TTTGGCGCGC ACCGGCGACC AACTACAGGG CATCAAGAAA GGTGTGCTGG AGCTGGCCGA CATCGTGGTC GTGAACAAGG CCGATGGGGA ACATCTGCGC GATGCGCGTT TGGCCGCCAG GGAGCTGTCC GGCGCTATCA GGCTGATCTA CCCACGCGAA ACAATCTGGC GGCCACCGGT TCTCACGATG AGCGCGGTGG AAGGAACCGG TCTGACACAG CTGGGGGAAG CTGTCGAGCG CCATCGCCAA GTGCTCACCG AGGCCGGTGA GTTCGAAGCG CGCCGACGCG AACAGCAAGT GGACTGGACA TGGCAGATGG TGCGAGATGC GGTGCTGGAC CGAGTGCTGT CCAACCCGGA CGTGCGCAAG ATCCGAGCCG ACGTCGAGCG CCGGGTTCGG GCGGGGGAGC TGACTCCCGC TCTGGCCGCC CAGCAAATTT TGGATACCGC GCAGTCC
Details for MyulA.00200.b.A1.GE29660
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSPDDAK VEQLAAAVRN GDRAALPRAI TLLESTRADD RRQVQELLLA LLPDSGKAHR VGITGVPGVG KSTTIEALGM YLIEQGHKVA VLAVDPSSTR TGGSILGDKT RMARLAMHPD AYVRPSPTSG TLGGVAKATR ESVVLLEAAG FDVVLVETVG VGQSEVAVAN MVDTFVLLTL ARTGDQLQGI KKGVLELADI VVVNKADGEH LRDARLAARE LSGAIRLIYP RETIWRPPVL TMSAVEGTGL TQLGEAVERH RQVXTEAGEF EARRXEQQVX WXWXMXRDXX XTECCPXXXV RKIRAXVXRX XRXGXXXXXL AXXXXXXXXX XXXXXXAXXX XXXXXXTXXX XX
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggtg acctccccgg acgacgccaa ggtcgagcaa ctggccgccg ccgtgcgcaa tggcgaccgt gccgcgctgc cacgggcgat cacgctgctg gagtccaccc gcgccgacga tcggcggcag gtccaagagc tgttgctggc actgctcccc gactccggga aggcccatcg ggtgggtatt accggcgtcc cgggagtggg gaagtccacc acgatcgagg ccctgggcat gtacctcatc gagcagggcc acaaggtggc cgtgctggcg gtggacccct cgtcgacgcg taccggcgga tcgattctgg gcgacaagac gcggatggcg cggttggcaa tgcacccgga tgcctacgtc cggccctctc cgacgtcggg aactctgggc ggcgtagcaa aggcaacgcg ggaatcggta gtgctcttgg aagccgccgg cttcgacgtg gttctggtcg aaacggtcgg cgtcggccag tccgaagtgg ccgtcgcaaa catggtcgac accttcgtct tgctgacttt ggcgcgcacc ggcgaccaac tacagggcat caagaaaggt gtgctggagc tggccgacat cgtggtcgtg aacaaggccg atggggaaca tctgcgcgat gcgcgtttgg ccgccaggga gctgtccggc gctatcaggc tgatctaccc acgcgaaaca atctggcggc caccggttct cacgatgagc gcggtggaag gaaccggtct gacacagctg ggggaagctg tcgagcgcca tcgccaagtg ntcaccgagg ccggtgagtt cgaagcgcgc cgacncgaac agcaagtggn ctggncatgg nagatggngc gagatgnnng ctgnaccgag tgctgtccnn cccngnacgt gcgcaagatc cgagccgang tcnancgnnn ggnncggnng gggnnctgnn nccnnnnnct ngcnncccng cnantttnnn nncnnnnann cnnaanannn ngannnnntg cannnnntnn nnnnnnnnnn ngntancnaa ccnnnaannn ntnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSPDDAK VEQLAAAVRN GDRAALPRAI TLLESTRADD RRQVQELLLA LLPDSGKAHR VGITGVPGVG KSTTIEALGM YLIEQGHKVA VLAVDPSSTR TGGSILGDKT RMARLAMHPD AYVRPSPTSG TLGGVAKATR ESVVLLEAAG FDVVLVETVG VGQSEVAVAN MVDTFVLLTL ARTGDQLQGI KKGVLELADI VVVNKADGEH LRDARLAARE LSGAIRLIYP RETIWRPPVL TMSAVEGTGL TQLGEAVERH RQVLTEAGEF EARRREQQVD WTWQMVRDAV LDRVLSNPDV RKIRADVERR VRAGELTPAL AAQQILDTAQ S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacctcc ccggacgacg ccaaggtcga gcaactggcc gccgccgtgc gcaatggcga ccgtgccgcg ctgccacggg cgatcacgct gctggagtcc acccgcgccg acgatcggcg gcaggtccaa gagctgttgc tggcactgct ccccgactcc gggaaggccc atcgggtggg tattaccggc gtcccgggag tggggaagtc caccacgatc gaggccctgg gcatgtacct catcgagcag ggccacaagg tggccgtgct ggcggtggac ccctcgtcga cgcgtaccgg cggatcgatt ctgggcgaca agacgcggat ggcgcggttg gcaatgcacc cggatgccta cgtccggccc tctccgacgt cgggaactct gggcggcgta gcaaaggcaa cgcgggaatc ggtagtgctc ttggaagccg ccggcttcga cgtggttctg gtcgaaacgg tcggcgtcgg ccagtccgaa gtggccgtcg caaacatggt cgacaccttc gtcttgctga ctttggcgcg caccggcgac caactacagg gcatcaagaa aggtgtgctg gagctggccg acatcgtggt cgtgaacaag gccgatgggg aacatctgcg cgatgcgcgt ttggccgcca gggagctgtc cggcgctatc aggctgatct acccacgcga aacaatctgg cggccaccgg ttctcacgat gagcgcggtg gaaggaaccg gtctgacaca gctgggggaa gctgtcgagc gccatcgcca agtgctcacc gaggccggtg agttcgaagc gcgccgacgc gaacagcaag tggactggac atggcagatg gtgcgagatg cggtgctgga ccgagtgctg tccaacccgg acgtgcgcaa gatccgagcc gacgtcgagc gccgggttcg ggcgggggag ctgactcccg ctctggccgc ccagcaaatt ttggataccg cgcagtccaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa