MythA.00809.a
Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase) (Rv1658 ortholog)
| CENTER ID: | MythA.00809.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00809.a.B1.GE37642 | full length | 1 | 400 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4U7J
DEPOSITED: 7/30/2014
DETERMINATION: XRay
CLONE: MythA.00809.a.B1.GE37642
PROTEIN: MythA.00809.a.B1.PW37508
4XFJ
DEPOSITED: 12/27/2014
DETERMINATION: XRay
CLONE: MythA.00809.a.B1.GE37642
PROTEIN: MythA.00809.a.B1.PW37508
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.372 |
| RefSeq: | WP_003923805.1 |
| UniProt: | G7CBN9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSERVILAYS GGLDTSVAIS WIGKETGREV VAVAIDLGQG GEDMEVVRQR ALDCGAVESI VIDARDEFAN DYCVPAIQSN ALYMDRYPLV SALSRPLIVK HLVKAAREHG GTIVAHGCTG KGNDQVRFEV GFASLAPDLE VLAPVRDYAW TREKAIAFAE ENNIPINVTK RSPFSIDQNV WGRAVETGFL EHLWNAPTKD VYSYTEDPTV NWSTPDEVIV GFEQGVPVSI DGRSVTPLQA IEELNRRGGE QGVGRLDVVE DRLVGIKSRE IYEAPGAMVL ITAHTELEHV TLERELGRFK RITDQKWGEL VYDGLWFSPL KTALESFVAK TQEHVTGEIR MVLHGGHIAV NGRRSPKSLY DFNLATYDEG DTFDQSAAKG FVQIHGLSSS ISARRDLQGQ
NT Sequence
atgtccgaac gcgtcatctt ggcgtactcc ggcggtctcg acacctcggt ggcgatcagc tggatcggca aggagacggg tcgcgaggtg gtggcggtgg ccatcgacct ggggcagggt ggtgaggaca tggaggtcgt ccgccagcgc gccctggact gcggcgcggt ggagtcgatc gtcatcgacg cccgcgacga gttcgccaac gactactgcg tccccgcgat ccagtccaat gccctctaca tggaccgcta cccgctggtg tcggcgctga gccgaccgtt gatcgtcaag cacctggtca aggcggcccg agagcacggc ggcaccatcg tcgcgcacgg ctgcaccggc aagggcaacg accaggtccg cttcgaggtc gggttcgcct cactggcgcc ggatctcgag gtgctggcgc cggtgcggga ttacgcctgg acccgggaga aggcgatcgc gttcgccgag gagaacaaca tccccatcaa cgtcaccaag cggtcgccgt tctccatcga ccagaacgtc tggggccgcg cggtggagac cggcttcctg gagcatctgt ggaacgcccc gaccaaggac gtctacagct acaccgagga cccgaccgtc aactggagca cccccgacga ggtgatcgtc ggtttcgagc agggtgtgcc ggtgtccatc gacggccggt cggtgacgcc gctgcaggcg atcgaggagc tgaaccggcg cggcggggaa cagggtgtcg gccgcctcga cgtggtcgag gaccggctgg tgggcatcaa gagccgggag atctacgagg cgcccggcgc gatggtgctc atcaccgcgc acaccgagct ggaacacgtc acgctggaac gtgagctcgg ccggttcaag cgcatcaccg accagaagtg gggcgagctg gtctacgacg gtctgtggtt ctcaccgttg aagaccgcgc tggagtcgtt cgtcgccaag acccaggagc acgtcaccgg tgagatccgg atggtgttgc acggcggcca catcgcggtc aacgggcgcc gcagcccgaa gtcgctgtac gacttcaacc tggcgaccta cgacgagggc gacaccttcg accagtcggc ggccaagggc ttcgtgcaga tccacggcct gtcgtcgagc atctcggccc gccgggacct gcagggccag tga
Details for MythA.00809.a.B1.GE37642
| HARVESTED ON: | 1/13/2014 |
| SEQUENCED ON: | 4/14/2017 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVVRQRAL DCGAVESIVI DARDEFANDY CVPAIQSNAL YMDRYPLVSA LSRPLIVKHL VKAAREHGGT IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN NIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY SYTEDPTVNW STPDEVIVGF EQGVPVSIDG RSVTPLQAIE ELNRRGGEQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRFKRI TDQKWGELVY DGLWFSPLKT ALESFVAKTQ EHVTGEIRMV LHGGHIAVNG RRSPKSLYDF NLATYDEGDT FDQSAAKGFV QIHGLSS
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc gaacgcgtca tcttggcgta ctccggcggt ctcgacacct cggtggcgat cagctggatc ggcaaggaga cgggtcgcga ggtggtggcg gtggccatcg acctggggca gggtggtgag gacatggagg tcgtccgcca gcgcgccctg gactgcggcg cggtggagtc gatcgtcatc gacgcccgcg acgagttcgc caacgactac tgcgtccccg cgatccagtc caatgccctc tacatggacc gctacccgct ggtgtcggcg ctgagccgac cgttgatcgt caagcacctg gtcaaggcgg cccgagagca cggcggcacc atcgtcgcgc acggctgcac cggcaagggc aacgaccagg tccgcttcga ggtcgggttc gcctcactgg cgccggatct cgaggtgctg gcgccggtgc gggattacgc ctggacccgg gagaaggcga tcgcgttcgc cgaggagaac aacatcccca tcaacgtcac caagcggtcg ccgttctcca tcgaccagaa cgtctggggc cgcgcggtgg agaccggctt cctggagcat ctgtggaacg ccccgaccaa ggacgtctac agctacaccg aggacccgac cgtcaactgg agcacccccg acgaggtgat cgtcggtttc gagcagggtg tgccggtgtc catcgacggc cggtcggtga cgccgctgca ggcgatcgag gagctgaacc ggcgcggcgg ggaacagggt gtcggccgcc tcgacgtggt cgaggaccgg ctggtgggca tcaagagccg ggagatctac gaggcgcccg gcgcgatggt gctcatcacc gcgcacaccg agctggaaca cgtcacgctg gaacgtgagc tcggccggtt caagcgcatc accgaccaga agtggggcga gctggtctac gacggtctgt ggttctcacc gttgaagacc gcgctggagt cgttcgtcgc caagacccag gagcacgtca ccggtgagat ccggatggtg ttgcacggcg gccacatcgc ggtcaacggg cgccgcagcc cgaagtcgct gtacgacttc aacctggcga cctacgacga gggcgacacc ttcgaccagt cggcggccaa gggcttcgtg cagatccacg gcctgtcgtc gagc
Expected Protein Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVVRQRAL DCGAVESIVI DARDEFANDY CVPAIQSNAL YMDRYPLVSA LSRPLIVKHL VKAAREHGGT IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN NIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY SYTEDPTVNW STPDEVIVGF EQGVPVSIDG RSVTPLQAIE ELNRRGGEQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRFKRI TDQKWGELVY DGLWFSPLKT ALESFVAKTQ EHVTGEIRMV LHGGHIAVNG RRSPKSLYDF NLATYDEGDT FDQSAAKGFV QIHGLSSSIS ARRDLQGQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aacgcgtcat cttggcgtac tccggcggtc tcgacacctc ggtggcgatc agctggatcg gcaaggagac gggtcgcgag gtggtggcgg tggccatcga cctggggcag ggtggtgagg acatggaggt cgtccgccag cgcgccctgg actgcggcgc ggtggagtcg atcgtcatcg acgcccgcga cgagttcgcc aacgactact gcgtccccgc gatccagtcc aatgccctct acatggaccg ctacccgctg gtgtcggcgc tgagccgacc gttgatcgtc aagcacctgg tcaaggcggc ccgagagcac ggcggcacca tcgtcgcgca cggctgcacc ggcaagggca acgaccaggt ccgcttcgag gtcgggttcg cctcactggc gccggatctc gaggtgctgg cgccggtgcg ggattacgcc tggacccggg agaaggcgat cgcgttcgcc gaggagaaca acatccccat caacgtcacc aagcggtcgc cgttctccat cgaccagaac gtctggggcc gcgcggtgga gaccggcttc ctggagcatc tgtggaacgc cccgaccaag gacgtctaca gctacaccga ggacccgacc gtcaactgga gcacccccga cgaggtgatc gtcggtttcg agcagggtgt gccggtgtcc atcgacggcc ggtcggtgac gccgctgcag gcgatcgagg agctgaaccg gcgcggcggg gaacagggtg tcggccgcct cgacgtggtc gaggaccggc tggtgggcat caagagccgg gagatctacg aggcgcccgg cgcgatggtg ctcatcaccg cgcacaccga gctggaacac gtcacgctgg aacgtgagct cggccggttc aagcgcatca ccgaccagaa gtggggcgag ctggtctacg acggtctgtg gttctcaccg ttgaagaccg cgctggagtc gttcgtcgcc aagacccagg agcacgtcac cggtgagatc cggatggtgt tgcacggcgg ccacatcgcg gtcaacgggc gccgcagccc gaagtcgctg tacgacttca acctggcgac ctacgacgag ggcgacacct tcgaccagtc ggcggccaag ggcttcgtgc agatccacgg cctgtcgtcg agcatctcgg cccgccggga cctgcagggc cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat