MyleA.00809.a

Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase) (Rv1658 ortholog)

CENTER ID: MyleA.00809.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00809.a.B1.GE37458 full length 1 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2624
RefSeq: YP_002503635.1
UniProt: A0A0H3MZQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMPERIILAY SGGLDTSVAI NWIGKETSHE VVAVVIDLGQ GGEDMEVVRQ RALDCGAVEA IVVDARDEFA EGYCLPTVLN NALYMDRYPL VSAISRPLIV KHLVAAARAH GGSIVAHGCT GKGNDQVRFE VGFASLAPDL EILAPVRDYA WTREKAIAFA EENAIPINVT KRSPFSIDQN VWGRAVETGF LEHLWHAPTK EVYSYTDDPT INWNTPDEVI VGFEHGVPVS IDGSPVSMLG AIEALNRRAG AQGVGRLDVV EDRLVGIKSR EIYEAPGAMV LITAHAELEH VTLERELGRF KRQTDRRWAE LVYDGLWYSP LKTALESFVA ATQQHVTGEV RMVLHGGHIA VNGRRSAESL YDFNLATYDE GDTFDQSAAR GFVYVYGLPS KLAARRDLR
NT Sequence
ttgatgccgg aacgtattat cctggcgtat tcgggggggc tggatacctc ggtggcgatc aactggatcg gcaaggaaac tagccatgag gtcgtggcag tggtgattga tctcggccag ggcggcgagg acatggaggt cgtacgtcaa cgggcgttgg actgcggagc tgttgaggct atcgttgtcg atgcccgtga cgagttcgct gaaggctatt gcttgcccac cgttttgaac aacgcgctgt atatggatcg gtacccgttg gtgtccgcga tcagcaggcc gctgatcgtc aagcacctgg tcgcggccgc acgtgcgcac ggcggcagca tcgtcgcgca cggttgtacc ggcaagggta acgaccaggt ccgattcgaa gtcggattcg cttcgctggc accggatctc gaaatattgg cgccggtgcg cgactacgca tggacacgag agaaggcgat cgctttcgcc gaagagaacg ccatccccat caatgtcacc aaacgttcgc cgttctcgat tgaccagaat gtttggggcc gcgcagtgga aaccggtttc ctggaacact tgtggcacgc acctactaaa gaggtttatt cctacactga cgaccccacc atcaattgga acacgcccga tgaggtgatc gtcggttttg agcacggagt tccggtgtcg attgacggta gtccggtgtc catgctgggc gccatcgagg cactcaaccg acgcgctggc gcgcaaggcg tcgggcgtct cgacgttgtc gaagaccggc tggtaggcat caagagccgc gagatttacg aggcacccgg agcaatggtg ctcatcaccg cgcacgccga actcgagcac gtcacgctgg agcgtgagtt gggacgcttc aagcgtcaga ccgatcgacg ctgggccgaa ttggtgtatg acggattgtg gtattcgccg ctgaagaccg cactggagag ttttgtcgcc gctacgcaac aacacgttac cggtgaagtt cgaatggtgt tacatggagg ccatattgcg gtgaatgggc ggcgcagcgc cgaatcccta tacgatttca atttggccac ctacgacgaa ggggatacct tcgatcaatc cgctgctcgc ggcttcgtct acgtgtacgg gttaccgtcg aagctcgcgg cacgtaggga cttacggtga
Details for MyleA.00809.a.B1.GE37458
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHML MPERIILAYS GGLDTSVAIN WIGKETSHEV VAVVIDLGQG GEDMEVVRQR ALDCGAVEAI VVDARDEFAE GYCLPTVLNN ALYMDRYPLV SAISRPLIVK HLVAAARAHG GSIVAHGCTG KGNDQVRFEV GFASLAPDLE ILAPVRDYAW TREKAIAFAE ENAIPINVTK RSPFSIDQNV WGRAVETGFL EHLWHAPTKE VYSYTDDPTI NWNTPDEVIV GFEHGVPVSI DGSPVSMLGA IEALNRRAGA QGVXRLDVVE XRLVXIKSRE IYEAPGAMVL ITXHAELEHV TLXREXGRFK RQXDRRXXXX CXXXXXXXXX XXLXXFVXLX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nnnnnnnttt gtttnnttta agaaggagaa taccatggct caccaccacc accaccatat gttgatgccg gaacgtatta tcctggcgta ttcggggggg ctggatacct cggtggcgat caactggatc ggcaaggaaa ctagccatga ggtcgtggca gtggtgattg atctcggcca gggcggcgag gacatggagg tcgtacgtca acgggcgttg gactgcggag ctgttgaggc tatcgttgtc gatgcccgtg acgagttcgc tgaaggctat tgcttgccca ccgttttgaa caacgcgctg tatatggatc ggtacccgtt ggtgtccgcg atcagcaggc cgctgatcgt caagcacctg gtcgcggccg cacgtgcgca cggcggcagc atcgtcgcgc acggttgtac cggcaagggt aacgaccagg tccgattcga agtcggattc gcttcgctgg caccggatct cgaaatattg gcgccggtgc gcgactacgc atggacacga gagaaggcga tcgctttcgc cgaagagaac gccatcccca tcaatgtcac caaacgttcg ccgttctcga ttgaccagaa tgtttggggc cgcgcagtgg aaaccggttt cctggaacac ttgtggcacg cacctactaa agaggtttat tcctacactg acgaccccac catcaattgg aacacgcccg atgaggtgat cgtcggtttt gagcacggag ttccggtgtc gattgacggt agtccggtgt ccatgctggg cgccatcgag gcactcaacc gacgcgctgg cgcgcaaggc gtcnggcgtc tcgacgttgt cgaagancgg ctggtangca tcaagagccg cgagatttac gaggcacccg gagcaatggt gctcatcacc ncgcacgcng aactcgagca cgtcacgctg gancgtgagt ngggacgctt caagcgtcag ancgatcgac gctngnnnaa nnngtgtntg angnnnnnng nantnnnnnn nangannnca ctgganantt ttgtcncnct ncncanannc nnnnnnngnn nanntnnnnn ncntgnnnnn nnnntnnnnn nnnngnnngn nnnnnnnnnn annncnnnnn anntnnnnnn nnnnnnnnnn nnnnnnnnnn nnannnnncn nnntnnnnnn nnnnnnnnng ntnnc
Expected Protein Sequence
MAHHHHHHMM PERIILAYSG GLDTSVAINW IGKETSHEVV AVVIDLGQGG EDMEVVRQRA LDCGAVEAIV VDARDEFAEG YCLPTVLNNA LYMDRYPLVS AISRPLIVKH LVAAARAHGG SIVAHGCTGK GNDQVRFEVG FASLAPDLEI LAPVRDYAWT REKAIAFAEE NAIPINVTKR SPFSIDQNVW GRAVETGFLE HLWHAPTKEV YSYTDDPTIN WNTPDEVIVG FEHGVPVSID GSPVSMLGAI EALNRRAGAQ GVGRLDVVED RLVGIKSREI YEAPGAMVLI TAHAELEHVT LERELGRFKR QTDRRWAELV YDGLWYSPLK TALESFVAAT QQHVTGEVRM VLHGGHIAVN GRRSAESLYD FNLATYDEGD TFDQSAARGF VYVYGLPSKL AARRDLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgatgc cggaacgtat tatcctggcg tattcggggg ggctggatac ctcggtggcg atcaactgga tcggcaagga aactagccat gaggtcgtgg cagtggtgat tgatctcggc cagggcggcg aggacatgga ggtcgtacgt caacgggcgt tggactgcgg agctgttgag gctatcgttg tcgatgcccg tgacgagttc gctgaaggct attgcttgcc caccgttttg aacaacgcgc tgtatatgga tcggtacccg ttggtgtccg cgatcagcag gccgctgatc gtcaagcacc tggtcgcggc cgcacgtgcg cacggcggca gcatcgtcgc gcacggttgt accggcaagg gtaacgacca ggtccgattc gaagtcggat tcgcttcgct ggcaccggat ctcgaaatat tggcgccggt gcgcgactac gcatggacac gagagaaggc gatcgctttc gccgaagaga acgccatccc catcaatgtc accaaacgtt cgccgttctc gattgaccag aatgtttggg gccgcgcagt ggaaaccggt ttcctggaac acttgtggca cgcacctact aaagaggttt attcctacac tgacgacccc accatcaatt ggaacacgcc cgatgaggtg atcgtcggtt ttgagcacgg agttccggtg tcgattgacg gtagtccggt gtccatgctg ggcgccatcg aggcactcaa ccgacgcgct ggcgcgcaag gcgtcgggcg tctcgacgtt gtcgaagacc ggctggtagg catcaagagc cgcgagattt acgaggcacc cggagcaatg gtgctcatca ccgcgcacgc cgaactcgag cacgtcacgc tggagcgtga gttgggacgc ttcaagcgtc agaccgatcg acgctgggcc gaattggtgt atgacggatt gtggtattcg ccgctgaaga ccgcactgga gagttttgtc gccgctacgc aacaacacgt taccggtgaa gttcgaatgg tgttacatgg aggccatatt gcggtgaatg ggcggcgcag cgccgaatcc ctatacgatt tcaatttggc cacctacgac gaaggggata ccttcgatca atccgctgct cgcggcttcg tctacgtgta cgggttaccg tcgaagctcg cggcacgtag ggacttacgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat