SSGCID: Target Page

MyabA.00809.a

Argininosuccinate synthase (Rv1658 ortholog)

CENTER ID:	MyabA.00809.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00809.a.B1.GE37296	full length		1	404

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00809.a.B1.PS01937	full length		1	404

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2385
RefSeq:	YP_001703077.1
UniProt:	B1MB02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVEHSDRVI LAYSGGLDTS VAISWIGKET GREVVAVAID LGQGGEDMEV VRQRALDCGA VEAVVVDARD EFAEEYCLPT IQSNALYMDR YPLVSAISRP LIVKHLVAAA REHGGGIVAH GCTGKGNDQV RFEVGFASLA PDLEVLAPVR DYAWTREKAI AFAAENEIPI NVTKKSPFSI DQNVWGRAVE TGFLEDLWNA PTKDVYDYTE DPTLNWGAPD ELIVTFDKGV PTAIDGHPVS VLEAIVELNR RAGAQGVGRL DVVEDRLVGI KSREIYEAPG AMVLITAHEE LEHVTLEREL GRYKRRTDQK WAELVYDGLW YSPLKRSLEA FVADTQQHVS GDIRLVLHGG NIAVNGRRSP ESLYDFNLAT YDEGDSFDQS SAKGFVHIHG LSSKISAQRD LSGK

NT Sequence

atgcccgtag aacactctga ccgcgtaatc ctcgcctact cgggcggtct cgatacttcc gtggcgatca gctggatcgg caaggagacc ggccgtgagg tcgtcgccgt cgccatcgat ctgggccagg gcggcgagga catggaagtg gtgcgccagc gcgccctgga ctgcggcgcg gttgaggccg ttgtggtgga cgcacgcgac gaattcgccg aggaatactg cctacccacg atccaatcca acgcgctgta catggatcgg tacccgctgg tgtccgcgat cagccgtccg ctgatcgtca agcacctggt tgccgcggca cgcgagcacg gtggcgggat cgtcgctcac ggctgcaccg gcaagggcaa cgatcaggtg cgcttcgagg ttggctttgc ctcgctagca ccagatttgg aagtactggc gccggtgcgt gactacgcgt ggacccgcga gaaggcgatc gcgttcgcgg cagagaacga gattccgatc aacgtgacga agaagtcgcc gttctcgatc gatcagaacg tatggggacg cgcggtggaa accggcttcc tggaagacct ttggaacgcg ccgaccaaag atgtgtacga ctacaccgaa gacccgaccc tgaactgggg cgcacccgac gagctgatcg tcaccttcga caagggtgta ccaactgcca tcgacggcca cccggtcagc gtgttggagg ccatcgtcga gctcaaccgc cgggcgggtg cacagggcgt gggtcgcctg gacgtggtcg aagaccggct ggtcggcatc aagagccgtg aaatctatga ggcgccgggt gcgatggttc tgatcaccgc acacgaggag ctcgaacacg tcaccctgga gcgcgaactt ggccgctaca agcgacgcac ggaccagaag tgggccgagc tggtgtacga cgggctctgg tactcgccgc tgaagcgttc actggaggcc tttgtcgccg atacccagca gcatgtgtcc ggcgacatcc ggctggtgct gcacggcggc aacatcgccg tcaatggccg ccgcagtcct gaatccctgt acgacttcaa cctggcgacc tacgacgagg gcgacagctt cgatcagtcc agcgccaagg gcttcgtgca tattcacggt ctgtcctcca agatctccgc gcagagggac ctctcgggca agtga

Details for MyabA.00809.a.B1.GE37296

HARVESTED ON:	8/28/2013
SEQUENCED ON:	4/14/2017
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP VEHSDRVILA YSGGLDTSVA ISWIGKETGR EVVAVAIDLG QGGEDMEVVR QRALDCGAVE AVVVDARDEF AEEYCLPTIQ SNALYMDRYP LVSAISRPLI VKHLVAAARE HGGGIVAHGC TGKGNDQVRF EVGFASLAPD LEVLAPVRDY AWTREKAIAF AAENEIPINV TKKSPFSIDQ NVWGRAVETG FLEDLWNAPT KDVYDYTEDP TLNWGAPDEL IVTFDKGVPT AIDGHPVSVL EAIVELNRRA GAQGVGRLDV VEDRLVGIKS REIYEAPGAM VLITAHEELE HVTLERELGR YKRRTDQKWA ELVYDGLWYS PLKRSLEAFV ADTQQHVSGD IRLVLHGGNI AVNGRRSPES LYDFNLATYD EGDSFDQSSA KGFVHIHGLS SK

Validated NT Sequence

atggctcacc accaccacca ccatatgccc gtagaacact ctgaccgcgt aatcctcgcc tactcgggcg gtctcgatac ttccgtggcg atcagctgga tcggcaagga gaccggccgt gaggtcgtcg ccgtcgccat cgatctgggc cagggcggcg aggacatgga agtggtgcgc cagcgcgccc tggactgcgg cgcggttgag gccgttgtgg tggacgcacg cgacgaattc gccgaggaat actgcctacc cacgatccaa tccaacgcgc tgtacatgga tcggtacccg ctggtgtccg cgatcagccg tccgctgatc gtcaagcacc tggttgccgc ggcacgcgag cacggtggcg ggatcgtcgc tcacggctgc accggcaagg gcaacgatca ggtgcgcttc gaggttggct ttgcctcgct agcaccagat ttggaagtac tggcgccggt gcgtgactac gcgtggaccc gcgagaaggc gatcgcgttc gcggcagaga acgagattcc gatcaacgtg acgaagaagt cgccgttctc gatcgatcag aacgtatggg gacgcgcggt ggaaaccggc ttcctggaag acctttggaa cgcgccgacc aaagatgtgt acgactacac cgaagacccg accctgaact ggggcgcacc cgacgagctg atcgtcacct tcgacaaggg tgtaccaact gccatcgacg gccacccggt cagcgtgttg gaggccatcg tcgagctcaa ccgccgggcg ggtgcacagg gcgtgggtcg cctggacgtg gtcgaagacc ggctggtcgg catcaagagc cgtgaaatct atgaggcgcc gggtgcgatg gttctgatca ccgcacacga ggagctcgaa cacgtcaccc tggagcgcga acttggccgc tacaagcgac gcacggacca gaagtgggcc gagctggtgt acgacgggct ctggtactcg ccgctgaagc gttcactgga ggcctttgtc gccgataccc agcagcatgt gtccggcgac atccggctgg tgctgcacgg cggcaacatc gccgtcaatg gccgccgcag tcctgaatcc ctgtacgact tcaacctggc gacctacgac gagggcgaca gcttcgatca gtccagcgcc aagggcttcg tgcatattca cggtctgtcc tccaagatc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg tagaacactc tgaccgcgta atcctcgcct actcgggcgg tctcgatact tccgtggcga tcagctggat cggcaaggag accggccgtg aggtcgtcgc cgtcgccatc gatctgggcc agggcggcga ggacatggaa gtggtgcgcc agcgcgccct ggactgcggc gcggttgagg ccgttgtggt ggacgcacgc gacgaattcg ccgaggaata ctgcctaccc acgatccaat ccaacgcgct gtacatggat cggtacccgc tggtgtccgc gatcagccgt ccgctgatcg tcaagcacct ggttgccgcg gcacgcgagc acggtggcgg gatcgtcgct cacggctgca ccggcaaggg caacgatcag gtgcgcttcg aggttggctt tgcctcgcta gcaccagatt tggaagtact ggcgccggtg cgtgactacg cgtggacccg cgagaaggcg atcgcgttcg cggcagagaa cgagattccg atcaacgtga cgaagaagtc gccgttctcg atcgatcaga acgtatgggg acgcgcggtg gaaaccggct tcctggaaga cctttggaac gcgccgacca aagatgtgta cgactacacc gaagacccga ccctgaactg gggcgcaccc gacgagctga tcgtcacctt cgacaagggt gtaccaactg ccatcgacgg ccacccggtc agcgtgttgg aggccatcgt cgagctcaac cgccgggcgg gtgcacaggg cgtgggtcgc ctggacgtgg tcgaagaccg gctggtcggc atcaagagcc gtgaaatcta tgaggcgccg ggtgcgatgg ttctgatcac cgcacacgag gagctcgaac acgtcaccct ggagcgcgaa cttggccgct acaagcgacg cacggaccag aagtgggccg agctggtgta cgacgggctc tggtactcgc cgctgaagcg ttcactggag gcctttgtcg ccgataccca gcagcatgtg tccggcgaca tccggctggt gctgcacggc ggcaacatcg ccgtcaatgg ccgccgcagt cctgaatccc tgtacgactt caacctggcg acctacgacg agggcgacag cttcgatcag tccagcgcca agggcttcgt gcatattcac ggtctgtcct ccaagatctc cgcgcagagg gacctctcgg gcaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyabA.00809.a.B1.PS01937

PURIFICATION DATe:	11/19/2013
CONCENTRATION:	44mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCG TAGAACACTC TGACCGCGTA ATCCTCGCCT ACTCGGGCGG TCTCGATACT TCCGTGGCGA TCAGCTGGAT CGGCAAGGAG ACCGGCCGTG AGGTCGTCGC CGTCGCCATC GATCTGGGCC AGGGCGGCGA GGACATGGAA GTGGTGCGCC AGCGCGCCCT GGACTGCGGC GCGGTTGAGG CCGTTGTGGT GGACGCACGC GACGAATTCG CCGAGGAATA CTGCCTACCC ACGATCCAAT CCAACGCGCT GTACATGGAT CGGTACCCGC TGGTGTCCGC GATCAGCCGT CCGCTGATCG TCAAGCACCT GGTTGCCGCG GCACGCGAGC ACGGTGGCGG GATCGTCGCT CACGGCTGCA CCGGCAAGGG CAACGATCAG GTGCGCTTCG AGGTTGGCTT TGCCTCGCTA GCACCAGATT TGGAAGTACT GGCGCCGGTG CGTGACTACG CGTGGACCCG CGAGAAGGCG ATCGCGTTCG CGGCAGAGAA CGAGATTCCG ATCAACGTGA CGAAGAAGTC GCCGTTCTCG ATCGATCAGA ACGTATGGGG ACGCGCGGTG GAAACCGGCT TCCTGGAAGA CCTTTGGAAC GCGCCGACCA AAGATGTGTA CGACTACACC GAAGACCCGA CCCTGAACTG GGGCGCACCC GACGAGCTGA TCGTCACCTT CGACAAGGGT GTACCAACTG CCATCGACGG CCACCCGGTC AGCGTGTTGG AGGCCATCGT CGAGCTCAAC CGCCGGGCGG GTGCACAGGG CGTGGGTCGC CTGGACGTGG TCGAAGACCG GCTGGTCGGC ATCAAGAGCC GTGAAATCTA TGAGGCGCCG GGTGCGATGG TTCTGATCAC CGCACACGAG GAGCTCGAAC ACGTCACCCT GGAGCGCGAA CTTGGCCGCT ACAAGCGACG CACGGACCAG AAGTGGGCCG AGCTGGTGTA CGACGGGCTC TGGTACTCGC CGCTGAAGCG TTCACTGGAG GCCTTTGTCG CCGATACCCA GCAGCATGTG TCCGGCGACA TCCGGCTGGT GCTGCACGGC GGCAACATCG CCGTCAATGG CCGCCGCAGT CCTGAATCCC TGTACGACTT CAACCTGGCG ACCTACGACG AGGGCGACAG CTTCGATCAG TCCAGCGCCA AGGGCTTCGT GCATATTCAC GGTCTGTCCT CCAAGATCTC CGCGCAGAGG GACCTCTCGG GCAAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT