MyavA.00809.a

Argininosuccinate synthase (Rv1658 ortholog)

CENTER ID: MyavA.00809.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00809.a.B1.GE37405 full length 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00809.a.B1.PW37485 full length 1 398

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3043
RefSeq: YP_882296.1
UniProt: A0QHA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSERVILAYS GGLDTSVAIS WIGKETGREV VAVAIDLGQG GEDMEVIRQR ALDCGAVEAV VVDARDEFAE GYCLPTIRNN ALYMDRYPLV SAISRPLIVK HLVAAAREHG GSIVAHGCTG KGNDQVRFEV GFASLAPDLE VLAPVRDYAW TREKAIAFAE ENAIPINVTK RSPFSIDQNV WGRAVETGFL EHLWNAPTKD VYAYTEDPTL NWSTPDEVIV GFERGVPVSI DGKPVSVLGA IEELNARAGA QGVGRLDVVE DRLVGIKSRE IYEAPGAMVL ITAHTELEHV TLERELGRFK RHTDQRWAEL VYDGLWYSPL KAALESFVDK TQEHVTGEIR MVLHGGHIAV NGRRSAESLY DFNLATYDEG DSFDQSAAKG FVYVHGLSSK IASRRDQR
NT Sequence
atgtcagaac gcgtcatcct ggcgtattcc ggcggtctgg acacctcggt ggccatcagc tggatcggca aggagaccgg ccgtgaggtc gtagcggtgg cgatcgacct cggccagggc ggcgaagaca tggaggtcat ccgccagcgc gccctggact gcggcgcggt ggaggccgtc gtcgtggacg cccgcgacga attcgccgag ggctattgcc tgcccaccat ccgcaacaac gccctctaca tggaccgcta cccgctggtg tcggcgatca gccggccgct gatcgtcaag cacctggtgg ccgcggcccg cgagcacggc ggcagcatcg tcgcgcacgg ctgcaccggc aagggcaacg accaggtccg cttcgaggtc ggattcgctt cgctggcacc ggatctcgag gtgctggccc cggtgcgcga ttacgcgtgg acgcgggaga aggcgatcgc gttcgccgag gagaacgcca tcccgatcaa cgtgaccaaa cgctcgccat tctccatcga ccagaacgtg tggggtcgcg cggtggaaac cggtttcctg gaacaccttt ggaacgcgcc caccaaggac gtctacgcct acaccgagga ccccacgctg aactggagca cccccgacga ggtgatcgtc gggttcgagc gcggggttcc ggtgtccatc gacggcaagc cggtgtcggt gctgggcgcc atcgaggaac tcaacgcccg cgccggcgcc cagggggtgg ggcgcctgga cgtcgtggag gaccggctgg tgggcatcaa gagccgcgag atctacgagg cgcccggcgc catggtgctc atcaccgcgc acaccgagct cgagcacgtc acgctggagc gggagctggg ccggttcaag cggcacaccg atcagcgctg ggccgagctg gtgtacgacg ggttgtggta ttcgccgctg aaggctgcgc tggaaagctt cgtcgacaag acccaggagc acgtcaccgg cgagatccga atggtgttgc acggcggcca cattgccgtc aacggccggc gcagcgccga atcgctctac gacttcaacc tggccaccta cgacgagggc gacagcttcg accagtcggc ggcgaagggt ttcgtctacg tgcacgggct gtcgtcgaag atcgcctccc gccgggacca gcggtga
Details for MyavA.00809.a.B1.GE37405
HARVESTED ON: 9/27/2013
SEQUENCED ON: 4/14/2017
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVIRQRAL DCGAVEAVVV DARDEFAEGY CLPTIRNNAL YMDRYPLVSA ISRPLIVKHL VAAAREHGGS IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN AIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY AYTEDPTLNW STPDEVIVGF ERGVPVSIDG KPVSVLGAIE ELNARAGAQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRFKRH TDQRWAELVY DGLWYSPLKA ALESFVDKTQ EHVTGEIRMV LHGGHIAVNG RRSAESLYDF NLATYDEGDS FDQSAAKGFV YVHGLSS
Validated NT Sequence
atggctcacc accaccacca ccatatgtca gaacgcgtca tcctggcgta ttccggcggt ctggacacct cggtggccat cagctggatc ggcaaggaga ccggccgtga ggtcgtagcg gtggcgatcg acctcggcca gggcggcgaa gacatggagg tcatccgcca gcgcgccctg gactgcggcg cggtggaggc cgtcgtcgtg gacgcccgcg acgaattcgc cgagggctat tgcctgccca ccatccgcaa caacgccctc tacatggacc gctacccgct ggtgtcggcg atcagccggc cgctgatcgt caagcacctg gtggccgcgg cccgcgagca cggcggcagc atcgtcgcgc acggctgcac cggcaagggc aacgaccagg tccgcttcga ggtcggattc gcttcgctgg caccggatct cgaggtgctg gccccggtgc gcgattacgc gtggacgcgg gagaaggcga tcgcgttcgc cgaggagaac gccatcccga tcaacgtgac caaacgctcg ccattctcca tcgaccagaa cgtgtggggt cgcgcggtgg aaaccggttt cctggaacac ctttggaacg cgcccaccaa ggacgtctac gcctacaccg aggaccccac gctgaactgg agcacccccg acgaggtgat cgtcgggttc gagcgcgggg ttccggtgtc catcgacggc aagccggtgt cggtgctggg cgccatcgag gaactcaacg cccgcgccgg cgcccagggg gtggggcgcc tggacgtcgt ggaggaccgg ctggtgggca tcaagagccg cgagatctac gaggcgcccg gcgccatggt gctcatcacc gcgcacaccg agctcgagca cgtcacgctg gagcgggagc tgggccggtt caagcggcac accgatcagc gctgggccga gctggtgtac gacgggttgt ggtattcgcc gctgaaggct gcgctggaaa gcttcgtcga caagacccag gagcacgtca ccggcgagat ccgaatggtg ttgcacggcg gccacattgc cgtcaacggc cggcgcagcg ccgaatcgct ctacgacttc aacctggcca cctacgacga gggcgacagc ttcgaccagt cggcggcgaa gggtttcgtc tacgtgcacg ggctgtcgtc gaa
Expected Protein Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVIRQRAL DCGAVEAVVV DARDEFAEGY CLPTIRNNAL YMDRYPLVSA ISRPLIVKHL VAAAREHGGS IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN AIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY AYTEDPTLNW STPDEVIVGF ERGVPVSIDG KPVSVLGAIE ELNARAGAQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRFKRH TDQRWAELVY DGLWYSPLKA ALESFVDKTQ EHVTGEIRMV LHGGHIAVNG RRSAESLYDF NLATYDEGDS FDQSAAKGFV YVHGLSSKIA SRRDQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag aacgcgtcat cctggcgtat tccggcggtc tggacacctc ggtggccatc agctggatcg gcaaggagac cggccgtgag gtcgtagcgg tggcgatcga cctcggccag ggcggcgaag acatggaggt catccgccag cgcgccctgg actgcggcgc ggtggaggcc gtcgtcgtgg acgcccgcga cgaattcgcc gagggctatt gcctgcccac catccgcaac aacgccctct acatggaccg ctacccgctg gtgtcggcga tcagccggcc gctgatcgtc aagcacctgg tggccgcggc ccgcgagcac ggcggcagca tcgtcgcgca cggctgcacc ggcaagggca acgaccaggt ccgcttcgag gtcggattcg cttcgctggc accggatctc gaggtgctgg ccccggtgcg cgattacgcg tggacgcggg agaaggcgat cgcgttcgcc gaggagaacg ccatcccgat caacgtgacc aaacgctcgc cattctccat cgaccagaac gtgtggggtc gcgcggtgga aaccggtttc ctggaacacc tttggaacgc gcccaccaag gacgtctacg cctacaccga ggaccccacg ctgaactgga gcacccccga cgaggtgatc gtcgggttcg agcgcggggt tccggtgtcc atcgacggca agccggtgtc ggtgctgggc gccatcgagg aactcaacgc ccgcgccggc gcccaggggg tggggcgcct ggacgtcgtg gaggaccggc tggtgggcat caagagccgc gagatctacg aggcgcccgg cgccatggtg ctcatcaccg cgcacaccga gctcgagcac gtcacgctgg agcgggagct gggccggttc aagcggcaca ccgatcagcg ctgggccgag ctggtgtacg acgggttgtg gtattcgccg ctgaaggctg cgctggaaag cttcgtcgac aagacccagg agcacgtcac cggcgagatc cgaatggtgt tgcacggcgg ccacattgcc gtcaacggcc ggcgcagcgc cgaatcgctc tacgacttca acctggccac ctacgacgag ggcgacagct tcgaccagtc ggcggcgaag ggtttcgtct acgtgcacgg gctgtcgtcg aagatcgcct cccgccggga ccagcggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MyavA.00809.a.B1.PW37485
PURIFICATION DATe: 4/28/2014
CONCENTRATION: 22.2mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVIRQRAL DCGAVEAVVV DARDEFAEGY CLPTIRNNAL YMDRYPLVSA ISRPLIVKHL VAAAREHGGS IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN AIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY AYTEDPTLNW STPDEVIVGF ERGVPVSIDG KPVSVLGAIE ELNARAGAQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRFKRH TDQRWAELVY DGLWYSPLKA ALESFVDKTQ EHVTGEIRMV LHGGHIAVNG RRSAESLYDF NLATYDEGDS FDQSAAKGFV YVHGLSS
Validated NT Sequence
atggctcacc accaccacca ccatatgtca gaacgcgtca tcctggcgta ttccggcggt ctggacacct cggtggccat cagctggatc ggcaaggaga ccggccgtga ggtcgtagcg gtggcgatcg acctcggcca gggcggcgaa gacatggagg tcatccgcca gcgcgccctg gactgcggcg cggtggaggc cgtcgtcgtg gacgcccgcg acgaattcgc cgagggctat tgcctgccca ccatccgcaa caacgccctc tacatggacc gctacccgct ggtgtcggcg atcagccggc cgctgatcgt caagcacctg gtggccgcgg cccgcgagca cggcggcagc atcgtcgcgc acggctgcac cggcaagggc aacgaccagg tccgcttcga ggtcggattc gcttcgctgg caccggatct cgaggtgctg gccccggtgc gcgattacgc gtggacgcgg gagaaggcga tcgcgttcgc cgaggagaac gccatcccga tcaacgtgac caaacgctcg ccattctcca tcgaccagaa cgtgtggggt cgcgcggtgg aaaccggttt cctggaacac ctttggaacg cgcccaccaa ggacgtctac gcctacaccg aggaccccac gctgaactgg agcacccccg acgaggtgat cgtcgggttc gagcgcgggg ttccggtgtc catcgacggc aagccggtgt cggtgctggg cgccatcgag gaactcaacg cccgcgccgg cgcccagggg gtggggcgcc tggacgtcgt ggaggaccgg ctggtgggca tcaagagccg cgagatctac gaggcgcccg gcgccatggt gctcatcacc gcgcacaccg agctcgagca cgtcacgctg gagcgggagc tgggccggtt caagcggcac accgatcagc gctgggccga gctggtgtac gacgggttgt ggtattcgcc gctgaaggct gcgctggaaa gcttcgtcga caagacccag gagcacgtca ccggcgagat ccgaatggtg ttgcacggcg gccacattgc cgtcaacggc cggcgcagcg ccgaatcgct ctacgacttc aacctggcca cctacgacga gggcgacagc ttcgaccagt cggcggcgaa gggtttcgtc tacgtgcacg ggctgtcgtc gaa
Expressed Protein Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVIRQRAL DCGAVEAVVV DARDEFAEGY CLPTIRNNAL YMDRYPLVSA ISRPLIVKHL VAAAREHGGS IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN AIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY AYTEDPTLNW STPDEVIVGF ERGVPVSIDG KPVSVLGAIE ELNARAGAQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRFKRH TDQRWAELVY DGLWYSPLKA ALESFVDKTQ EHVTGEIRMV LHGGHIAVNG RRSAESLYDF NLATYDEGDS FDQSAAKGFV YVHGLSSKIA SRRDQR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAG AACGCGTCAT CCTGGCGTAT TCCGGCGGTC TGGACACCTC GGTGGCCATC AGCTGGATCG GCAAGGAGAC CGGCCGTGAG GTCGTAGCGG TGGCGATCGA CCTCGGCCAG GGCGGCGAAG ACATGGAGGT CATCCGCCAG CGCGCCCTGG ACTGCGGCGC GGTGGAGGCC GTCGTCGTGG ACGCCCGCGA CGAATTCGCC GAGGGCTATT GCCTGCCCAC CATCCGCAAC AACGCCCTCT ACATGGACCG CTACCCGCTG GTGTCGGCGA TCAGCCGGCC GCTGATCGTC AAGCACCTGG TGGCCGCGGC CCGCGAGCAC GGCGGCAGCA TCGTCGCGCA CGGCTGCACC GGCAAGGGCA ACGACCAGGT CCGCTTCGAG GTCGGATTCG CTTCGCTGGC ACCGGATCTC GAGGTGCTGG CCCCGGTGCG CGATTACGCG TGGACGCGGG AGAAGGCGAT CGCGTTCGCC GAGGAGAACG CCATCCCGAT CAACGTGACC AAACGCTCGC CATTCTCCAT CGACCAGAAC GTGTGGGGTC GCGCGGTGGA AACCGGTTTC CTGGAACACC TTTGGAACGC GCCCACCAAG GACGTCTACG CCTACACCGA GGACCCCACG CTGAACTGGA GCACCCCCGA CGAGGTGATC GTCGGGTTCG AGCGCGGGGT TCCGGTGTCC ATCGACGGCA AGCCGGTGTC GGTGCTGGGC GCCATCGAGG AACTCAACGC CCGCGCCGGC GCCCAGGGGG TGGGGCGCCT GGACGTCGTG GAGGACCGGC TGGTGGGCAT CAAGAGCCGC GAGATCTACG AGGCGCCCGG CGCCATGGTG CTCATCACCG CGCACACCGA GCTCGAGCAC GTCACGCTGG AGCGGGAGCT GGGCCGGTTC AAGCGGCACA CCGATCAGCG CTGGGCCGAG CTGGTGTACG ACGGGTTGTG GTATTCGCCG CTGAAGGCTG CGCTGGAAAG CTTCGTCGAC AAGACCCAGG AGCACGTCAC CGGCGAGATC CGAATGGTGT TGCACGGCGG CCACATTGCC GTCAACGGCC GGCGCAGCGC CGAATCGCTC TACGACTTCA ACCTGGCCAC CTACGACGAG GGCGACAGCT TCGACCAGTC GGCGGCGAAG GGTTTCGTCT ACGTGCACGG GCTGTCGTCG AAGATCGCCT CCCGCCGGGA CCAGCGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT