MymaA.00809.a

Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase) (Rv1658 ortholog)

CENTER ID: MymaA.00809.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00809.a.B1.GE37194 full length 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2629
RefSeq: YP_001850773.1
UniProt: B2HR32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSERVILAYS GGLDTSVAIS WIGKETGREV VAVAIDLGQG GEDMEVVRQR ALDCGAVEAV VVDARDEFAE GYCLPTILNN ALYMDRYPLV SAISRPLIVK HLVEAAREHG GGIVAHGCTG KGNDQVRFEV GFASLAPDLE VLAPVRDYAW TREKAIAFAE ENAIPINVTK RSPFSIDQNV WGRAVETGFL EHLWNAPTKD VYSYTEDPTV NWNTPDEVIV GFERGMPVSI DGNAVTMLQA IEELNRRAGA QGVGRLDVVE DRLVGIKSRE IYEAPGAMVL ITAHAELEHV TLERELARFK RHTDQRWAEL VYDGLWYSPL KTALESFVAK TQEHVSGEIR LVLHGGHIAV NGRRSAESLY DFNLATYDEG DTFDQSAAKG FVYVHGLSSK LSARRDLQ
NT Sequence
atgtcagaac gcgtcatcct ggcgtattcc ggcggtctgg acacctcggt ggccatcagc tggataggca aggagaccgg ccgtgaggtc gtggctgttg cgattgacct cggccagggt ggcgaggaca tggaggtcgt gcgtcagcga gcgctcgatt gcggtgccgt cgaggcggtc gtggtcgatg ctcgcgacga gttcgcggag ggctactgcc tgcccaccat cttgaacaat gccctctaca tggaccgcta tccgctggtg tcggccatca gccggccgct gatcgtcaag catctggtgg aggcggcacg cgagcacggc ggtgggatcg tcgcgcacgg gtgcacaggc aagggcaatg accaggtgcg atttgaggtc gggttcgcgt cgctggctcc cgacctagag gtgttggcac cggttcgcga ctacgcctgg acccgggaga aggcgatcgc attcgccgaa gagaacgcga tcccgatcaa cgtcaccaag cgctcgccgt tctcgatcga ccaaaacgtg tggggccgag ctgtcgaaac cggcttcctc gagcacctgt ggaacgcgcc caccaaggac gtctactcct acaccgaaga tccgaccgtc aactggaaca cccccgatga ggtgatcgtc ggcttcgaac gcggcatgcc ggtgtcgatc gacggcaacg cggtcacgat gctgcaggcc atcgaggagc tcaaccgccg ggccggggcc cagggtgtcg ggcgcctcga tgtcgtcgag gaccgcttgg tgggcatcaa gagccgggag atctacgagg cgcccggggc catggtgctc atcacggccc acgccgagct cgagcacgtc accctcgagc gtgagctggc ccgattcaag cggcacaccg atcagcgctg ggccgagctg gtgtacgacg gactgtggta ttcgccgttg aagaccgcgc tggagagctt tgtcgccaag acccaggagc acgtgtctgg tgagatccgg ctggtgctgc acggcggtca catcgccgtc aacggccggc gcagcgcgga gtcgctctac gacttcaacc tggccaccta cgacgagggc gacaccttcg accagtcggc tgccaagggg ttcgtctacg tgcacggctt gtcgtccaaa ctctccgccc gccgggatct gcagtga
Details for MymaA.00809.a.B1.GE37194
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MEVVRQRALD CGAVEAVVVD ARDEFAEGYC LPTILNNALY MDRYPLVSAI SRPLIVKHLV EAAREHGGGI VAHGCTGKGN DQVRFEVGFA SLAPDLEVLA PVRDYAWTRE KAIAFAEENA IPINVTKRSP FSIDQNVWGR AVETGFLEHL WNAPTKDVYS YTEDPTVNWN TPDEVIVGFE RGMPVSIDGN AVTMLQAIEE LNRRAGAQGV GRLDVVEDRL VGIKSREIYE APGAMVLITA HAELEHVTLE RELARFKRHT DQRWAELVYD GLWYSPLKTA LESFVAKTQE HVSGEIRLVL HGGHIAVNGR RSAESLYDFN LATYDEGDTF DQSAAKGFVY VHGLSSKLSA R
Validated NT Sequence
ccggcgggcg gagagtttgg acgacaagcc gtgcacgtag acgaacccct tggcagccga ctggtcgaag gtgtcgccct cgtcgtaggt ggccaggttg aagtcgtaga gcgactccgc gctgcgccgg ccgttgacgg cgatgtgacc gccgtgcagc accagccgga tctcaccaga cacgtgctcc tgggtcttgg cgacaaagct ctccagcgcg gtcttcaacg gcgaatacca cagtccgtcg tacaccagct cggcccagcg ctgatcggtg tgccgcttga atcgggccag ctcacgctcg agggtgacgt gctcgagctc ggcgtgggcc gtgatgagca ccatggcccc gggcgcctcg tagatctccc ggctcttgat gcccaccaag cggtcctcga cgacatcgag gcgcccgaca ccctgggccc cggcccggcg gttgagctcc tcgatggcct gcagcatcgt gaccgcgttg ccgtcgatcg acaccggcat gccgcgttcg aagccgacga tcacctcatc gggggtgttc cagttgacgg tcggatcttc ggtgtaggag tagacgtcct tggtgggcgc gttccacagg tgctcgagga agccggtttc gacagctcgg ccccacacgt tttggtcgat cgagaacggc gagcgcttgg tgacgttgat cgggatcgcg ttctcttcgg cgaatgcgat cgccttctcc cgggtccagg cgtagtcgcg aaccggtgcc aacacctcta ggtcgggagc cagcgacgcg aacccgacct caaatcgcac ctggtcattg cccttgcctg tgcacccgtg cgcgacgatc ccaccgccgt gctcgcgtgc cgcctccacc agatgcttga cgatcagcgg ccggctgatg gccgacacca gcggatagcg gtccatgtag agggcattgt tcaagatggt gggcaggcag tagccctccg cgaactcgtc gcgagcatcg accacgaccg cctcgacggc accgcaatcg agcgctcgct gacgcacgac ctccatgtcc tcgccaccct ggccgaggtc aatcgcaaca gccacgacct cacggccggt ctccttgcct atccagctga tggccaccga ggtgtccaga ccgccggaat acgccaggat gacgc
Expected Protein Sequence
MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGREVVA VAIDLGQGGE DMEVVRQRAL DCGAVEAVVV DARDEFAEGY CLPTILNNAL YMDRYPLVSA ISRPLIVKHL VEAAREHGGG IVAHGCTGKG NDQVRFEVGF ASLAPDLEVL APVRDYAWTR EKAIAFAEEN AIPINVTKRS PFSIDQNVWG RAVETGFLEH LWNAPTKDVY SYTEDPTVNW NTPDEVIVGF ERGMPVSIDG NAVTMLQAIE ELNRRAGAQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHAELEHVTL ERELARFKRH TDQRWAELVY DGLWYSPLKT ALESFVAKTQ EHVSGEIRLV LHGGHIAVNG RRSAESLYDF NLATYDEGDT FDQSAAKGFV YVHGLSSKLS ARRDLQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag aacgcgtcat cctggcgtat tccggcggtc tggacacctc ggtggccatc agctggatag gcaaggagac cggccgtgag gtcgtggctg ttgcgattga cctcggccag ggtggcgagg acatggaggt cgtgcgtcag cgagcgctcg attgcggtgc cgtcgaggcg gtcgtggtcg atgctcgcga cgagttcgcg gagggctact gcctgcccac catcttgaac aatgccctct acatggaccg ctatccgctg gtgtcggcca tcagccggcc gctgatcgtc aagcatctgg tggaggcggc acgcgagcac ggcggtggga tcgtcgcgca cgggtgcaca ggcaagggca atgaccaggt gcgatttgag gtcgggttcg cgtcgctggc tcccgaccta gaggtgttgg caccggttcg cgactacgcc tggacccggg agaaggcgat cgcattcgcc gaagagaacg cgatcccgat caacgtcacc aagcgctcgc cgttctcgat cgaccaaaac gtgtggggcc gagctgtcga aaccggcttc ctcgagcacc tgtggaacgc gcccaccaag gacgtctact cctacaccga agatccgacc gtcaactgga acacccccga tgaggtgatc gtcggcttcg aacgcggcat gccggtgtcg atcgacggca acgcggtcac gatgctgcag gccatcgagg agctcaaccg ccgggccggg gcccagggtg tcgggcgcct cgatgtcgtc gaggaccgct tggtgggcat caagagccgg gagatctacg aggcgcccgg ggccatggtg ctcatcacgg cccacgccga gctcgagcac gtcaccctcg agcgtgagct ggcccgattc aagcggcaca ccgatcagcg ctgggccgag ctggtgtacg acggactgtg gtattcgccg ttgaagaccg cgctggagag ctttgtcgcc aagacccagg agcacgtgtc tggtgagatc cggctggtgc tgcacggcgg tcacatcgcc gtcaacggcc ggcgcagcgc ggagtcgctc tacgacttca acctggccac ctacgacgag ggcgacacct tcgaccagtc ggctgccaag gggttcgtct acgtgcacgg cttgtcgtcc aaactctccg cccgccggga tctgcagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at