SSGCID: Target Page

MyfoA.00809.a

Argininosuccinate synthase (EC 6.3.4.5) (Rv1658 ortholog)

CENTER ID:	MyfoA.00809.a
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.00809.a.B1.GE37903	full length		1	399

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.00809.a.B1.PW37496	full length		1	399

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.3763
RefSeq:	WP_003885157.1
UniProt:	K0V223

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSERVILAYS GGLDTSVAIS WIGKETGKEV VAVAIDLGQG GEDMDVVRQR ALDCGAVEAV VVDARDEFAD EYCLPTIKAN ALYMDRYPLV SAISRPLIVK HLVDAARSHG GTVVAHGCTG KGNDQVRFEV GFASLAPELD VIAPVRDYAW TREKAIAFAE ENAIPINVTK RSPFSIDQNV WGRAVETGFL EDLWNAPTKD VYDYTQDPTV NFNAPDELII SFDKGRPIAI DGRPLSVLEI IQELNTRAGA QGVGRLDVVE DRLVGIKSRE IYEAPGAMVL ITAHTELEHV TLERELGRYK RGVDQKWGEL TYDGLWFSPL KRSLEAFVEH TQEHVSGDIR LVLHAGAIIV NGRRSGESLY DFNLATYDEG DSFDQSAAKG FVQLHGLSSK ISAKRDLGL

NT Sequence

atgtccgaac gcgtcattct ggcgtactcc ggaggtctgg acacctcggt cgcgatcagc tggatcggca aggagaccgg caaagaggtc gtcgccgtcg ccatcgacct cggccagggc ggcgaggaca tggatgtcgt acgccagcgt gccctcgact gcggtgcggt cgaggcggtc gtggtcgacg cccgtgacga attcgccgac gaatactgcc tgccgaccat caaggccaac gcgctctaca tggaccgcta tccgctggtg tcggccatca gccgcccgct gatcgtcaag catctggtgg atgccgcccg cagccacggc ggcaccgtcg tcgcccacgg ctgcaccggc aagggcaacg accaggtccg cttcgaggtc ggattcgcct ccctggcacc cgaactggac gtcatcgcgc ccgtccgcga ctacgcctgg acccgcgaga aggccatcgc gttcgccgag gagaacgcga tcccgatcaa cgtcaccaag cgctcaccgt tctccatcga ccagaacgtg tggggccgcg cggtggagac cgggttcctc gaggacctgt ggaacgcccc gaccaaggac gtctacgact acacccagga cccgacggtc aacttcaacg cgcccgacga gctgatcatc agcttcgaca agggccgccc gatcgcgatc gacggtcgcc cgctcagcgt gctggagatc atccaggagc tcaacacccg ggccggtgcc cagggcgtgg gccgcctcga tgtggtggaa gaccggctgg tcggcatcaa gagccgcgag atctatgagg ccccgggcgc gatggtgctg atcaccgcgc acaccgaact cgagcacgtg accttggagc gcgagctcgg ccggtacaag cgcggcgtgg accagaagtg gggcgagctc acctacgacg gcctctggtt cagcccgctg aagcgttcgc tggaggcgtt cgtcgagcac acgcaggagc acgtctcggg cgacatccgg ctggtgctgc acgccggagc catcatcgtc aacggccggc gctcgggcga gtcgctgtac gacttcaacc ttgccaccta cgacgagggc gacagcttcg accagagcgc ggccaagggc ttcgtgcagc tgcacggcct gagctccaag atctcggcca agcgcgacct gggtctgtaa

Details for MyfoA.00809.a.B1.GE37903

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	84

Validated AA Sequence

MDVVRQRALD CGAVEAVVVD ARDEFADEYC LPTIKANALY MDRYPLVSAI SRPLIVKHLV DAARSHGGTV VAHGCTGKGN DQVRFEVGFA SLAPELDVIA PVRDYAWTRE KAIAFAEENA IPINVTKRSP FSIDQNVWGR AVETGFLEDL WNAPTKDVYD YTQDPTVNFN APDELIISFD KGRPIAIDGR PLSVLEIIQE LNTRAGAQGV GRLDVVEDRL VGIKSREIYE APGAMVLITA HTELEHVTLE RELGRYKRGV DQKWGELTYD GLWFSPLKRS LEAFVEHTQE HVSGDIRLVX HAGAIIVNGR RSGESLYDFN LATYDEGDSF DQSAAKGFVQ LHG

Validated NT Sequence

aggccgtgca gctgcacgaa gcccttggcc gcgctctggt cgaagctgtc gccctcgtcg tangtggcaa ggttgaagtc gtacagcgac tcgcccgagc gccggccgtt gacgatgatg gctccggcgt gcancaccag ccggatgtcg cccgagacgt gctcctgcgt gtgctcgacg aacgcctcca gcgaacgctt cagcgggctg aaccagaggc cgtcgtaggt gagctcgccc cacttctggt ccacgccgcg cttgtaccgg ccgagctcgc gctccaaggt cacgtgctcg agttcggtgt gcgcggtgat cagcaccatc gcgcccgggg cctcatagat ctcgcggctc ttgatgccga ccagccggtc ttccaccaca tcgaggcggc ccacgccctg ggcaccggcc cgggtgttga gctcctggat gatctccagc acgctgagcg ggcgaccgtc gatcgcgatc gggcggccct tgtcgaagct gatgatcagc tcgtcgggcg cgttgaagtt gaccgtcggg tcctgggtgt agtcgtagac gtccttggtc ggggcgttcc acaggtcctc gaggaacccg gtctccaccg cgcggcccca cacgttctgg tcgatggaga acggtgagcg cttggtgacg ttgatcggga tcgcgttctc ctcggcgaac gcgatggcct tctcgcgggt ccaggcgtag tcgcggacgg gcgcgatgac gtccagttcg ggtgccaggg aggcgaatcc gacctcgaag cggacctggt cgttgccctt gccggtgcag ccgtgggcga cgacggtgcc gccgtggctg cgggcggcat ccaccagatg cttgacgatc agcgggcggc tgatggccga caccagcgga tagcggtcca tgtagagcgc gttggccttg atggtcggca ggcagtattc gtcggcgaat tcgtcacggg cgtcgaccac gaccgcctcg accgcaccgc agtcgagggc acgctggcgt acgacatcca tgtcctcgcc gccctggccg aggtcgatgg cgacggcgac gacctctttg ccggtctcct tgccgatcca gctgatcgcg accgaggtgt ccagacctcc ggagtacgcc agaatgacgc gttcg

Expected Protein Sequence

MAHHHHHHMS ERVILAYSGG LDTSVAISWI GKETGKEVVA VAIDLGQGGE DMDVVRQRAL DCGAVEAVVV DARDEFADEY CLPTIKANAL YMDRYPLVSA ISRPLIVKHL VDAARSHGGT VVAHGCTGKG NDQVRFEVGF ASLAPELDVI APVRDYAWTR EKAIAFAEEN AIPINVTKRS PFSIDQNVWG RAVETGFLED LWNAPTKDVY DYTQDPTVNF NAPDELIISF DKGRPIAIDG RPLSVLEIIQ ELNTRAGAQG VGRLDVVEDR LVGIKSREIY EAPGAMVLIT AHTELEHVTL ERELGRYKRG VDQKWGELTY DGLWFSPLKR SLEAFVEHTQ EHVSGDIRLV LHAGAIIVNG RRSGESLYDF NLATYDEGDS FDQSAAKGFV QLHGLSSKIS AKRDLGL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aacgcgtcat tctggcgtac tccggaggtc tggacacctc ggtcgcgatc agctggatcg gcaaggagac cggcaaagag gtcgtcgccg tcgccatcga cctcggccag ggcggcgagg acatggatgt cgtacgccag cgtgccctcg actgcggtgc ggtcgaggcg gtcgtggtcg acgcccgtga cgaattcgcc gacgaatact gcctgccgac catcaaggcc aacgcgctct acatggaccg ctatccgctg gtgtcggcca tcagccgccc gctgatcgtc aagcatctgg tggatgccgc ccgcagccac ggcggcaccg tcgtcgccca cggctgcacc ggcaagggca acgaccaggt ccgcttcgag gtcggattcg cctccctggc acccgaactg gacgtcatcg cgcccgtccg cgactacgcc tggacccgcg agaaggccat cgcgttcgcc gaggagaacg cgatcccgat caacgtcacc aagcgctcac cgttctccat cgaccagaac gtgtggggcc gcgcggtgga gaccgggttc ctcgaggacc tgtggaacgc cccgaccaag gacgtctacg actacaccca ggacccgacg gtcaacttca acgcgcccga cgagctgatc atcagcttcg acaagggccg cccgatcgcg atcgacggtc gcccgctcag cgtgctggag atcatccagg agctcaacac ccgggccggt gcccagggcg tgggccgcct cgatgtggtg gaagaccggc tggtcggcat caagagccgc gagatctatg aggccccggg cgcgatggtg ctgatcaccg cgcacaccga actcgagcac gtgaccttgg agcgcgagct cggccggtac aagcgcggcg tggaccagaa gtggggcgag ctcacctacg acggcctctg gttcagcccg ctgaagcgtt cgctggaggc gttcgtcgag cacacgcagg agcacgtctc gggcgacatc cggctggtgc tgcacgccgg agccatcatc gtcaacggcc ggcgctcggg cgagtcgctg tacgacttca accttgccac ctacgacgag ggcgacagct tcgaccagag cgcggccaag ggcttcgtgc agctgcacgg cctgagctcc aagatctcgg ccaagcgcga cctgggtctg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for MyfoA.00809.a.B1.PW37496

PURIFICATION DATe:	5/19/2014
CONCENTRATION:	28.2mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	84

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG AACGCGTCAT TCTGGCGTAC TCCGGAGGTC TGGACACCTC GGTCGCGATC AGCTGGATCG GCAAGGAGAC CGGCAAAGAG GTCGTCGCCG TCGCCATCGA CCTCGGCCAG GGCGGCGAGG ACATGGATGT CGTACGCCAG CGTGCCCTCG ACTGCGGTGC GGTCGAGGCG GTCGTGGTCG ACGCCCGTGA CGAATTCGCC GACGAATACT GCCTGCCGAC CATCAAGGCC AACGCGCTCT ACATGGACCG CTATCCGCTG GTGTCGGCCA TCAGCCGCCC GCTGATCGTC AAGCATCTGG TGGATGCCGC CCGCAGCCAC GGCGGCACCG TCGTCGCCCA CGGCTGCACC GGCAAGGGCA ACGACCAGGT CCGCTTCGAG GTCGGATTCG CCTCCCTGGC ACCCGAACTG GACGTCATCG CGCCCGTCCG CGACTACGCC TGGACCCGCG AGAAGGCCAT CGCGTTCGCC GAGGAGAACG CGATCCCGAT CAACGTCACC AAGCGCTCAC CGTTCTCCAT CGACCAGAAC GTGTGGGGCC GCGCGGTGGA GACCGGGTTC CTCGAGGACC TGTGGAACGC CCCGACCAAG GACGTCTACG ACTACACCCA GGACCCGACG GTCAACTTCA ACGCGCCCGA CGAGCTGATC ATCAGCTTCG ACAAGGGCCG CCCGATCGCG ATCGACGGTC GCCCGCTCAG CGTGCTGGAG ATCATCCAGG AGCTCAACAC CCGGGCCGGT GCCCAGGGCG TGGGCCGCCT CGATGTGGTG GAAGACCGGC TGGTCGGCAT CAAGAGCCGC GAGATCTATG AGGCCCCGGG CGCGATGGTG CTGATCACCG CGCACACCGA ACTCGAGCAC GTGACCTTGG AGCGCGAGCT CGGCCGGTAC AAGCGCGGCG TGGACCAGAA GTGGGGCGAG CTCACCTACG ACGGCCTCTG GTTCAGCCCG CTGAAGCGTT CGCTGGAGGC GTTCGTCGAG CACACGCAGG AGCACGTCTC GGGCGACATC CGGCTGGTGC TGCACGCCGG AGCCATCATC GTCAACGGCC GGCGCTCGGG CGAGTCGCTG TACGACTTCA ACCTTGCCAC CTACGACGAG GGCGACAGCT TCGACCAGAG CGCGGCCAAG GGCTTCGTGC AGCTGCACGG CCTGAGCTCC AAGATCTCGG CCAAGCGCGA CCTGGGTCTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT