MysmA.01640.c

Glutaryl-CoA dehydrogenase

CENTER ID: MysmA.01640.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.01640.c.A1.PS01045 Full length( MysmA.01640.c ) 1 399

Structures

3SF6
DEPOSITED: 6/12/2011
DETERMINATION: XRay
CLONE: MysmA.01640.c.A1.GE28976
PROTEIN: MysmA.01640.c.A1.PS01045

Publications by SSGCID

Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_129299
PATRIC ID: fig|246196.19.peg.2431
RefSeq: YP_886806.1
UniProt: A0QV68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNSAVAQPR SQRRGADDLI GINAVLSAEE REIRDTVRSV VQRRIKPHIA SWYEDGELPA RELAVELGEL GLLGMHLKGY GCAGMSAVAY GLACLELEAG DSGIRSLVSV QGSLAMYAIH AFGSDEQKDQ WLPDMASGHR IGCFGLTEPD HGSDPAGMRT RATRSGDDWI LTGTKMWITN GSVADVAVVW ARTDEGIRGF VVPTDTPGFT ANTIKSKMSL RASVTSELVL DGVRLPDSAR LPGATSLGAP LRCLNEARFG IVFGALGAAR DCLETALAYA CSREQFDRPI GGFQLTQQKL ADMTLEYGKG FLLALHLGRQ KDAGELAPEQ VSLGKLNNVR EAIEIARTAR TVLGASGITG EYPVMRHANN LESVLTYEGT SEMHTLIIGQ ALTGVGAFR
NT Sequence
ATGACCAACA GCGCTGTCGC ACAACCTCGT TCGCAGCGCC GTGGCGCCGA CGACCTGATC GGGATCAACG CCGTGCTCAG CGCCGAGGAG CGCGAGATCC GCGACACCGT GCGCAGCGTC GTCCAGCGCA GGATCAAGCC GCACATCGCG TCCTGGTACG AGGACGGTGA GCTGCCCGCG CGCGAACTGG CCGTCGAACT GGGCGAACTC GGCCTCTTGG GCATGCACCT GAAGGGCTAC GGGTGCGCCG GGATGTCCGC GGTCGCATAC GGATTGGCGT GCCTTGAGCT CGAAGCGGGC GACTCGGGGA TCCGTTCGCT GGTGAGCGTC CAGGGGTCGT TGGCGATGTA CGCGATCCAC GCCTTCGGCA GCGACGAGCA GAAGGACCAG TGGTTGCCCG ACATGGCGAG CGGTCACCGC ATCGGGTGCT TCGGCCTCAC CGAACCCGAC CACGGTTCCG ACCCTGCGGG TATGCGCACC AGGGCCACCC GCTCAGGTGA CGACTGGATC CTCACCGGGA CCAAGATGTG GATCACCAAC GGGTCGGTGG CCGACGTCGC GGTCGTCTGG GCGCGTACCG ACGAGGGGAT CCGCGGATTC GTCGTCCCCA CCGACACCCC GGGATTCACC GCGAACACCA TCAAATCCAA GATGTCGCTG CGCGCCTCGG TGACCAGCGA GCTCGTTCTC GACGGGGTGC GGCTCCCCGA CAGCGCCAGG TTGCCCGGGG CGACGAGCCT GGGTGCGCCG CTGCGGTGTC TCAACGAGGC GCGGTTCGGG ATCGTCTTCG GTGCGCTCGG CGCGGCACGC GACTGCCTGG AGACGGCGCT GGCCTACGCC TGCTCACGCG AACAGTTCGA CCGCCCCATC GGCGGGTTCC AGCTCACTCA GCAGAAGCTC GCCGACATGA CGCTGGAGTA CGGCAAGGGA TTCCTGCTGG CGCTGCACCT CGGCCGGCAG AAAGACGCCG GTGAGCTGGC CCCCGAGCAG GTCAGCCTCG GCAAGCTCAA CAACGTGCGC GAAGCCATCG AGATCGCCCG CACCGCCCGC ACGGTGCTCG GCGCCAGCGG CATCACCGGG GAGTATCCCG TGATGCGGCA CGCCAACAAC CTCGAATCGG TGTTGACCTA CGAAGGCACC AGCGAGATGC ATACGCTGAT CATCGGGCAG GCACTCACCG GCGTCGGCGC CTTCCGC
Details for MysmA.01640.c.A1.PS01045
PURIFICATION DATe: 3/31/2011
CONCENTRATION: 45mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 67
Protocol Notes
notes unavailable
Validated AA Sequence
MYAIHAFGSD EQKDQWLPDM ASGHRIGCFG LTEPDHGSDP AGMRTRATRS GDDWILTGTK MWITNGSVAD VAVVWARTDE GIRGFVVPTD TPGFTANTIK SKMSLRASVT SELVLDGVRL PDSARLPGAT SLGAPLRCLN EARFGIVFGA LGAARDCLET ALAYACSREQ FDRPIGGFQL TQQKLADMTL EYGKGFLLAL HLGRQKDAGE LAPEQVSLGK LNNVREAIEI ARTARTVLGA SGITGEYPVM RHANNLESVL TYEGTSEMHT LIIGQALTGV GAFR
Validated NT Sequence
cnnnnnnnnn nnnngnnagc agcncggatc ctcgagaagc ttggctgcag aacttgttcg tgctgtttat tagcggaagg cgccgacgcc ggtgagtgcc tgcccgatga tcagcgtatg catctcgctg gtgccttcgt aggtcaacac cgattcgagg ttgttggcgt gccgcatcac gggatactcc ccggtgatgc cgctggcgcc gagcaccgtg cgggcggtgc gggcgatctc gatggcttcg cgcacgttgt tgagcttgcc gaggctgacc tgctcggggg ccagctcacc ggcgtctttc tgccggccga ggtgcagcgc cagcaggaat cccttgccgt actccagcgt catgtcggcg agcttctgct gagtgagctg gaacccgccg atggggcggt cgaactgttc gcgtgagcag gcgtaggcca gcgccgtctc caggcagtcg cgtgccgcgc cgagcgcacc gaagacgatc ccgaaccgcg cctcgttgag acaccgcagc ggcgcaccca ggctcgtcgc cccgggcaac ctggcgctgt cggggagccg caccccgtcg agaacgagct cgctggtcac cgaggcgcgc agcgacatct tggatttgat ggtgttcgcg gtgaatcccg gggtgtcggt ggggacgacg aatccgcgga tcccctcgtc ggtacgcgcc cagacgaccg cgacgtcngc caccgacccg ttggtgatcc acatcttggt cccggtgagg atccagtcgt cacctgagcg ggtggccctg gtgcgcatac ccgcagggtc ggaaccgtgg tcgggttcgg tgaggccgaa gcacccgatg cggtgaccgc tcgccatgtc gggcaaccac tggtccttct gctcgtcgct gccgaaggcg tggatcgcgt acatcgccna acgacccctg gnacgctcac cagcgaacgn atccccgant cgccngcttc gagctcnnna cgncnntccg tatgcnancg cnnacntncc ngngcnncnn natncnnngc atgnccnnnn nnnnnngncc nnnngannnn nnnnnnnnnn ggnnnntcan cnncnnnnnc nnnnnnnnnn nnnnngnnan nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nnnnnnnnnn anntnnnnnc ncnnnnnnnn g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSAVAQP RSQRRGADDL IGINAVLSAE EREIRDTVRS VVQRRIKPHI ASWYEDGELP ARELAVELGE LGLLGMHLKG YGCAGMSAVA YGLACLELEA GDSGIRSLVS VQGSLAMYAI HAFGSDEQKD QWLPDMASGH RIGCFGLTEP DHGSDPAGMR TRATRSGDDW ILTGTKMWIT NGSVADVAVV WARTDEGIRG FVVPTDTPGF TANTIKSKMS LRASVTSELV LDGVRLPDSA RLPGATSLGA PLRCLNEARF GIVFGALGAA RDCLETALAY ACSREQFDRP IGGFQLTQQK LADMTLEYGK GFLLALHLGR QKDAGELAPE QVSLGKLNNV REAIEIARTA RTVLGASGIT GEYPVMRHAN NLESVLTYEG TSEMHTLIIG QALTGVGAFR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAACAGC GCTGTCGCAC AACCTCGTTC GCAGCGCCGT GGCGCCGACG ACCTGATCGG GATCAACGCC GTGCTCAGCG CCGAGGAGCG CGAGATCCGC GACACCGTGC GCAGCGTCGT CCAGCGCAGG ATCAAGCCGC ACATCGCGTC CTGGTACGAG GACGGTGAGC TGCCCGCGCG CGAACTGGCC GTCGAACTGG GCGAACTCGG CCTCTTGGGC ATGCACCTGA AGGGCTACGG GTGCGCCGGG ATGTCCGCGG TCGCATACGG ATTGGCGTGC CTTGAGCTCG AAGCGGGCGA CTCGGGGATC CGTTCGCTGG TGAGCGTCCA GGGGTCGTTG GCGATGTACG CGATCCACGC CTTCGGCAGC GACGAGCAGA AGGACCAGTG GTTGCCCGAC ATGGCGAGCG GTCACCGCAT CGGGTGCTTC GGCCTCACCG AACCCGACCA CGGTTCCGAC CCTGCGGGTA TGCGCACCAG GGCCACCCGC TCAGGTGACG ACTGGATCCT CACCGGGACC AAGATGTGGA TCACCAACGG GTCGGTGGCC GACGTCGCGG TCGTCTGGGC GCGTACCGAC GAGGGGATCC GCGGATTCGT CGTCCCCACC GACACCCCGG GATTCACCGC GAACACCATC AAATCCAAGA TGTCGCTGCG CGCCTCGGTG ACCAGCGAGC TCGTTCTCGA CGGGGTGCGG CTCCCCGACA GCGCCAGGTT GCCCGGGGCG ACGAGCCTGG GTGCGCCGCT GCGGTGTCTC AACGAGGCGC GGTTCGGGAT CGTCTTCGGT GCGCTCGGCG CGGCACGCGA CTGCCTGGAG ACGGCGCTGG CCTACGCCTG CTCACGCGAA CAGTTCGACC GCCCCATCGG CGGGTTCCAG CTCACTCAGC AGAAGCTCGC CGACATGACG CTGGAGTACG GCAAGGGATT CCTGCTGGCG CTGCACCTCG GCCGGCAGAA AGACGCCGGT GAGCTGGCCC CCGAGCAGGT CAGCCTCGGC AAGCTCAACA ACGTGCGCGA AGCCATCGAG ATCGCCCGCA CCGCCCGCAC GGTGCTCGGC GCCAGCGGCA TCACCGGGGA GTATCCCGTG ATGCGGCACG CCAACAACCT CGAATCGGTG TTGACCTACG AAGGCACCAG CGAGATGCAT ACGCTGATCA TCGGGCAGGC ACTCACCGGC GTCGGCGCCT TCCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA