MymaA.01640.a

Acyl-CoA dehydrogenase FadE7

CENTER ID: MymaA.01640.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01640.a.A1.GE30229 Full length( MymaA.01640.a ) 1 395
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01640.a.A1.PS00927 Full length( MymaA.01640.a ) 1 395

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.749
RefSeq: YP_001849012.1
UniProt: B2HPY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTETPPTFD PHDPLGLDAS LSDDEIAVRD TVRKFCAEHI TAHVAEWFEN GDLPAARDLA KQLGGLGLLG MHLQGYGCGG ASAVHYGLAC LELEAADSGI RSLVSVQGSL AMFAIAHFGS EEHKQRWLPP MAAGELIGCF GLTEPDVGSD PAAMKTRARR DGSDWVLNGR KMWITNGSIA DVAIVWAATE EGIRGFVVPT ETAGFAANTI HHKLSLRASI TSELVLDDVR LPAEAMLPGA TGVRGPLTCL SEARYGIVWG AMGAARSAWQ CALDYAKERT QFGRPIAGFQ LTQAKLVDMA VELHKGQLLA LQLGRLKDSV GLRPEQVSFG KLNNTREAIK ICRTARTILG GNGISLEYPV IRHMVNLESV LTYEGTPEMH QLVLGQAFTG LAAFR
NT Sequence
ATGAGCACCG AGACACCGCC CACCTTCGAC CCGCACGACC CGCTCGGACT GGATGCTTCG CTATCCGACG ACGAGATCGC GGTCCGCGAC ACCGTAAGAA AGTTCTGCGC CGAGCACATC ACTGCCCATG TCGCCGAATG GTTCGAGAAC GGGGACTTGC CCGCCGCGCG CGACCTGGCC AAGCAGCTAG GCGGACTGGG CCTGCTCGGA ATGCATTTGC AGGGCTACGG CTGCGGCGGC GCGTCGGCCG TCCACTACGG CTTGGCGTGC CTGGAATTGG AAGCCGCCGA TTCCGGGATC CGGTCGCTGG TTTCGGTGCA GGGATCGCTG GCAATGTTCG CGATCGCACA CTTCGGCTCC GAGGAGCACA AGCAGCGGTG GTTGCCGCCC ATGGCCGCCG GTGAGCTGAT CGGCTGCTTC GGCCTGACCG AACCCGACGT CGGTTCCGAC CCGGCCGCGA TGAAAACCCG GGCGCGCCGC GATGGTTCGG ATTGGGTGCT CAACGGCCGC AAGATGTGGA TCACCAACGG CTCGATCGCC GACGTGGCGA TCGTGTGGGC CGCCACCGAG GAGGGAATCC GCGGCTTCGT CGTGCCCACC GAAACGGCCG GCTTCGCCGC CAACACCATC CACCACAAAC TGTCCCTGCG GGCATCGATC ACCAGCGAGT TGGTCCTCGA CGATGTGCGG TTGCCGGCCG AGGCGATGTT GCCCGGCGCG ACCGGCGTCA GGGGACCGTT GACCTGCTTG TCGGAGGCTC GCTACGGAAT CGTCTGGGGA GCGATGGGCG CGGCGCGTTC GGCCTGGCAA TGCGCGTTGG ACTACGCCAA AGAACGCACC CAATTCGGCC GTCCCATCGC CGGGTTTCAA TTGACCCAGG CCAAGCTGGT CGACATGGCG GTCGAACTGC ACAAGGGACA GCTCCTAGCG CTACAGCTCG GCCGCCTCAA AGACAGTGTC GGCCTGCGGC CCGAGCAGGT CAGCTTCGGC AAGCTCAACA ACACCCGGGA GGCGATCAAG ATCTGCCGGA CCGCCCGAAC CATACTGGGC GGCAACGGAA TTTCGCTCGA ATACCCGGTA ATTCGGCACA TGGTCAACCT GGAATCGGTA TTGACCTACG AGGGCACACC CGAGATGCAT CAGCTGGTGC TAGGGCAGGC TTTCACCGGT CTGGCCGCCT TCCGC
Details for MymaA.01640.a.A1.GE30229
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 77
Validated AA Sequence
MHLQGYGCGG ASAVHYGLAC LELEAADSGI RSLVSVQGSL AMFAIAHFGS EEHKQRWLPP MAAGELIGCF GLTEPDVGSD PAAMKTRARR DGSDWVLNGR KMWITNGSIA DVAIVWAATE EGIRGFVVPT ETAGFAANTI HHKLSLRASI TSELVLDDVR LPAEAMLPGA TGVRGPLTCL SEARYGIVWG AMGAARSAWQ CALDYAKERT QFGRPIAGFQ LTQAKLVDMA VELHKGQLLA LQLGRLKDSV GLRPEQVSFG KLNNTREAIK ICRTARTILG GNGISLEYPV IRHMVNLESV LTYEGTPEMH QLVLGQAFTG LAAF
Validated NT Sequence
ggaaggcggc cagaccggtg aaagcctgcc ctagcaccag ctgatgcatc tcgggtgtgc cctcgtaggt caataccgat tccaggttga ccatgtgccg aattaccggg tattcgagcg aaattccgtt gccgcccagt atggttcggg cggtccggca gatcttgatc gcctcccggg tgttgttgag cttgccgaag ctgacctgct cgggccgcag gccgacactg tctttgaggc ggccgagctg tagcgctagg agctgtccct tgtgcagttc gaccgccatg tcgaccagct tggcctgggt caattgaaac ccggcgatgg gacggccgaa ttgggtgcgt tctttggcgt agtccaacgc gcattgccag gccgaacgcg ccgcgcccat cgctccccag acgattccgt agcgagcctc cgacaagcag gtcaacggtc ccctgacgcc ggtcgcgccg ggcaacatcg cctcggccgg caaccgcaca tcgtcgagga ccaactcgct ggtgatcgat gcccgcaggg acagtttgtg gtggatggtg ttggcggcga agccggccgt ttcggtgggc acgacgaagc cgcggattcc ctcctcggtg gcggcccaca cgatcgccac gtcggcgatc gagccgttgg tgatccacat cttgcggccg ttgagcaccc aatccgaacc atcgcggcgc gcccgggttt tcatcgcggc cgggtcggaa ccgacgtcgg gttcggtcag gccgaagcag ccgatcagct caccggcggc catgggcggc aaccaccgct gcttgtgctc ctcggagccg aagtgtgcga tcgcgaacat tgccagcgat ccctgcaccg aaaccagcga ccggatcccg gaatcggcgg cttccaattc caggcacgcc aagccgtagt ggacggccga cgcgccgccg cagccgtagc cctgcaaatg cattccgagc aggcccagtc cgcctagctg cttggccagg tcgcgcgcgg cgggcaagtc cccgttctcg aaccattcgg cgacatgggc agtgatgtgc tcggcgcaga actttcttac ggtgtcgcgg accgcgatct cgtcgtcgga tagcgaagca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTETPPTF DPHDPLGLDA SLSDDEIAVR DTVRKFCAEH ITAHVAEWFE NGDLPAARDL AKQLGGLGLL GMHLQGYGCG GASAVHYGLA CLELEAADSG IRSLVSVQGS LAMFAIAHFG SEEHKQRWLP PMAAGELIGC FGLTEPDVGS DPAAMKTRAR RDGSDWVLNG RKMWITNGSI ADVAIVWAAT EEGIRGFVVP TETAGFAANT IHHKLSLRAS ITSELVLDDV RLPAEAMLPG ATGVRGPLTC LSEARYGIVW GAMGAARSAW QCALDYAKER TQFGRPIAGF QLTQAKLVDM AVELHKGQLL ALQLGRLKDS VGLRPEQVSF GKLNNTREAI KICRTARTIL GGNGISLEYP VIRHMVNLES VLTYEGTPEM HQLVLGQAFT GLAAFR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaccgag acaccgccca ccttcgaccc gcacgacccg ctcggactgg atgcttcgct atccgacgac gagatcgcgg tccgcgacac cgtaagaaag ttctgcgccg agcacatcac tgcccatgtc gccgaatggt tcgagaacgg ggacttgccc gccgcgcgcg acctggccaa gcagctaggc ggactgggcc tgctcggaat gcatttgcag ggctacggct gcggcggcgc gtcggccgtc cactacggct tggcgtgcct ggaattggaa gccgccgatt ccgggatccg gtcgctggtt tcggtgcagg gatcgctggc aatgttcgcg atcgcacact tcggctccga ggagcacaag cagcggtggt tgccgcccat ggccgccggt gagctgatcg gctgcttcgg cctgaccgaa cccgacgtcg gttccgaccc ggccgcgatg aaaacccggg cgcgccgcga tggttcggat tgggtgctca acggccgcaa gatgtggatc accaacggct cgatcgccga cgtggcgatc gtgtgggccg ccaccgagga gggaatccgc ggcttcgtcg tgcccaccga aacggccggc ttcgccgcca acaccatcca ccacaaactg tccctgcggg catcgatcac cagcgagttg gtcctcgacg atgtgcggtt gccggccgag gcgatgttgc ccggcgcgac cggcgtcagg ggaccgttga cctgcttgtc ggaggctcgc tacggaatcg tctggggagc gatgggcgcg gcgcgttcgg cctggcaatg cgcgttggac tacgccaaag aacgcaccca attcggccgt cccatcgccg ggtttcaatt gacccaggcc aagctggtcg acatggcggt cgaactgcac aagggacagc tcctagcgct acagctcggc cgcctcaaag acagtgtcgg cctgcggccc gagcaggtca gcttcggcaa gctcaacaac acccgggagg cgatcaagat ctgccggacc gcccgaacca tactgggcgg caacggaatt tcgctcgaat acccggtaat tcggcacatg gtcaacctgg aatcggtatt gacctacgag ggcacacccg agatgcatca gctggtgcta gggcaggctt tcaccggtct ggccgccttc cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MymaA.01640.a.A1.PS00927
PURIFICATION DATe: 1/3/2011
CONCENTRATION: 59.42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 77
Protocol Notes
notes unavailable
Validated AA Sequence
MHLQGYGCGG ASAVHYGLAC LELEAADSGI RSLVSVQGSL AMFAIAHFGS EEHKQRWLPP MAAGELIGCF GLTEPDVGSD PAAMKTRARR DGSDWVLNGR KMWITNGSIA DVAIVWAATE EGIRGFVVPT ETAGFAANTI HHKLSLRASI TSELVLDDVR LPAEAMLPGA TGVRGPLTCL SEARYGIVWG AMGAARSAWQ CALDYAKERT QFGRPIAGFQ LTQAKLVDMA VELHKGQLLA LQLGRLKDSV GLRPEQVSFG KLNNTREAIK ICRTARTILG GNGISLEYPV IRHMVNLESV LTYEGTPEMH QLVLGQAFTG LAAF
Validated NT Sequence
ggaaggcggc cagaccggtg aaagcctgcc ctagcaccag ctgatgcatc tcgggtgtgc cctcgtaggt caataccgat tccaggttga ccatgtgccg aattaccggg tattcgagcg aaattccgtt gccgcccagt atggttcggg cggtccggca gatcttgatc gcctcccggg tgttgttgag cttgccgaag ctgacctgct cgggccgcag gccgacactg tctttgaggc ggccgagctg tagcgctagg agctgtccct tgtgcagttc gaccgccatg tcgaccagct tggcctgggt caattgaaac ccggcgatgg gacggccgaa ttgggtgcgt tctttggcgt agtccaacgc gcattgccag gccgaacgcg ccgcgcccat cgctccccag acgattccgt agcgagcctc cgacaagcag gtcaacggtc ccctgacgcc ggtcgcgccg ggcaacatcg cctcggccgg caaccgcaca tcgtcgagga ccaactcgct ggtgatcgat gcccgcaggg acagtttgtg gtggatggtg ttggcggcga agccggccgt ttcggtgggc acgacgaagc cgcggattcc ctcctcggtg gcggcccaca cgatcgccac gtcggcgatc gagccgttgg tgatccacat cttgcggccg ttgagcaccc aatccgaacc atcgcggcgc gcccgggttt tcatcgcggc cgggtcggaa ccgacgtcgg gttcggtcag gccgaagcag ccgatcagct caccggcggc catgggcggc aaccaccgct gcttgtgctc ctcggagccg aagtgtgcga tcgcgaacat tgccagcgat ccctgcaccg aaaccagcga ccggatcccg gaatcggcgg cttccaattc caggcacgcc aagccgtagt ggacggccga cgcgccgccg cagccgtagc cctgcaaatg cattccgagc aggcccagtc cgcctagctg cttggccagg tcgcgcgcgg cgggcaagtc cccgttctcg aaccattcgg cgacatgggc agtgatgtgc tcggcgcaga actttcttac ggtgtcgcgg accgcgatct cgtcgtcgga tagcgaagca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTETPPTF DPHDPLGLDA SLSDDEIAVR DTVRKFCAEH ITAHVAEWFE NGDLPAARDL AKQLGGLGLL GMHLQGYGCG GASAVHYGLA CLELEAADSG IRSLVSVQGS LAMFAIAHFG SEEHKQRWLP PMAAGELIGC FGLTEPDVGS DPAAMKTRAR RDGSDWVLNG RKMWITNGSI ADVAIVWAAT EEGIRGFVVP TETAGFAANT IHHKLSLRAS ITSELVLDDV RLPAEAMLPG ATGVRGPLTC LSEARYGIVW GAMGAARSAW QCALDYAKER TQFGRPIAGF QLTQAKLVDM AVELHKGQLL ALQLGRLKDS VGLRPEQVSF GKLNNTREAI KICRTARTIL GGNGISLEYP VIRHMVNLES VLTYEGTPEM HQLVLGQAFT GLAAFR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCACCGAG ACACCGCCCA CCTTCGACCC GCACGACCCG CTCGGACTGG ATGCTTCGCT ATCCGACGAC GAGATCGCGG TCCGCGACAC CGTAAGAAAG TTCTGCGCCG AGCACATCAC TGCCCATGTC GCCGAATGGT TCGAGAACGG GGACTTGCCC GCCGCGCGCG ACCTGGCCAA GCAGCTAGGC GGACTGGGCC TGCTCGGAAT GCATTTGCAG GGCTACGGCT GCGGCGGCGC GTCGGCCGTC CACTACGGCT TGGCGTGCCT GGAATTGGAA GCCGCCGATT CCGGGATCCG GTCGCTGGTT TCGGTGCAGG GATCGCTGGC AATGTTCGCG ATCGCACACT TCGGCTCCGA GGAGCACAAG CAGCGGTGGT TGCCGCCCAT GGCCGCCGGT GAGCTGATCG GCTGCTTCGG CCTGACCGAA CCCGACGTCG GTTCCGACCC GGCCGCGATG AAAACCCGGG CGCGCCGCGA TGGTTCGGAT TGGGTGCTCA ACGGCCGCAA GATGTGGATC ACCAACGGCT CGATCGCCGA CGTGGCGATC GTGTGGGCCG CCACCGAGGA GGGAATCCGC GGCTTCGTCG TGCCCACCGA AACGGCCGGC TTCGCCGCCA ACACCATCCA CCACAAACTG TCCCTGCGGG CATCGATCAC CAGCGAGTTG GTCCTCGACG ATGTGCGGTT GCCGGCCGAG GCGATGTTGC CCGGCGCGAC CGGCGTCAGG GGACCGTTGA CCTGCTTGTC GGAGGCTCGC TACGGAATCG TCTGGGGAGC GATGGGCGCG GCGCGTTCGG CCTGGCAATG CGCGTTGGAC TACGCCAAAG AACGCACCCA ATTCGGCCGT CCCATCGCCG GGTTTCAATT GACCCAGGCC AAGCTGGTCG ACATGGCGGT CGAACTGCAC AAGGGACAGC TCCTAGCGCT ACAGCTCGGC CGCCTCAAAG ACAGTGTCGG CCTGCGGCCC GAGCAGGTCA GCTTCGGCAA GCTCAACAAC ACCCGGGAGG CGATCAAGAT CTGCCGGACC GCCCGAACCA TACTGGGCGG CAACGGAATT TCGCTCGAAT ACCCGGTAAT TCGGCACATG GTCAACCTGG AATCGGTATT GACCTACGAG GGCACACCCG AGATGCATCA GCTGGTGCTA GGGCAGGCTT TCACCGGTCT GGCCGCCTTC CGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA