SSGCID: Target Page

MyabA.01640.b

Putative acyl-CoA dehydrogenase FadE

CENTER ID:	MyabA.01640.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01640.b.A1.GE30538	Full length( MyabA.01640.b )		1	393

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4683
RefSeq:	YP_001705332.1
UniProt:	B1ML33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATDAAPTNP DELFGIDALL SDEERAIRDS VRALVQARVV PNVQRWYEHG DLPVRKLALE LGKIGVLGMH LHGYGCSGTS SLAYGLACME LEAGDSGIRS LVSVQGSLAM YAIYAYGSDA QKQKWLPRMA TGEELGCFGL TEPDFGSNPG GMRTHARRDG SDWVLNGTKM WITNGSIADV AVIWAQTDEG VRGFLVSTDS AGFDAQTIHS KMSLRASVTA ELNLVDVWVP EKNRLPLAVG LKAPLSCLNE ARFGIIFGSM GAARECLRTA LEYAQTREQF DRPIAGFQLT QEKLANMALE YGKGVLLALH LGRRKDADTL ATPQVSLGKL NNVREAIEIA RTARTILGAN GISGEYPVMR HANNLESVLT YEGTSEMHTL VIGQALTGIA AFR

NT Sequence

ATGGCTACCG ACGCTGCACC AACCAACCCC GACGAGTTGT TCGGTATCGA TGCGCTGCTC AGCGACGAGG AGCGCGCGAT CCGTGACAGC GTGCGCGCGC TGGTCCAGGC GCGCGTGGTA CCCAATGTTC AGCGCTGGTA CGAGCATGGT GATCTGCCGG TACGCAAGCT CGCGCTGGAG CTGGGCAAGA TCGGTGTACT CGGGATGCAT CTGCACGGAT ACGGATGTTC CGGAACGTCC TCTCTCGCAT ATGGACTGGC CTGCATGGAG CTGGAGGCCG GTGACTCGGG GATCCGCTCG TTGGTCAGTG TGCAGGGGTC GTTGGCGATG TATGCCATCT ATGCCTATGG CAGTGATGCG CAGAAGCAGA AGTGGCTGCC CAGGATGGCA ACGGGCGAAG AGCTGGGGTG TTTCGGTCTG ACCGAGCCCG ATTTCGGGTC CAACCCGGGC GGCATGCGCA CGCACGCGCG CCGGGATGGC AGCGACTGGG TACTCAATGG GACCAAGATG TGGATTACCA ACGGGTCGAT CGCCGATGTC GCGGTCATCT GGGCGCAGAC CGACGAGGGC GTCCGTGGTT TTCTCGTGTC GACCGATTCG GCGGGGTTCG ATGCCCAGAC CATCCATTCG AAGATGTCTT TGCGCGCATC CGTGACCGCC GAGCTCAACC TCGTTGATGT GTGGGTGCCG GAGAAGAACA GGCTGCCATT AGCCGTCGGG CTCAAGGCCC CGCTGTCGTG CCTCAACGAG GCGCGTTTTG GCATCATCTT CGGCTCGATG GGCGCCGCCC GTGAATGCTT GCGTACCGCA CTGGAATACG CGCAGACCAG GGAGCAGTTC GATCGCCCGA TCGCGGGCTT CCAGCTGACC CAAGAGAAAC TGGCCAACAT GGCGCTGGAA TACGGCAAGG GAGTGCTACT GGCGCTGCAT CTGGGGCGCC GCAAGGATGC CGACACTCTG GCGACCCCGC AGGTCAGCCT GGGCAAGCTC AACAATGTGC GCGAGGCCAT CGAGATAGCC CGGACCGCCC GCACAATCCT TGGTGCGAAC GGTATTTCGG GCGAGTACCC GGTGATGAGG CATGCCAACA ACCTGGAATC GGTGCTGACC TACGAGGGCA CCAGCGAGAT GCACACACTG GTTATCGGCC AGGCGCTGAC CGGGATTGCG GCGTTCCGC

Details for MyabA.01640.b.A1.GE30538

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	44kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttattagcgg aacgccgcaa tcccggtcag cgcctggccg ataaccagtg tgtgcatctc gctggtgccc tcgtaggtca gcaccgattc caggttgttg gcatgcctca tcaccgggta ctcgcccgaa ataccgttcg caccaaggat tgtgcgggcg gtccgggcta tctcgatggc ctcgcgcaca ttgttgagct tgcccaggct gacctgcggg gtcgccagag tgtcggcatc cttgcggcgc cccagatgca gcgccagtag cactcccttg ccgtattcca gcgccatgtt ggccagtttc tcttgggtca gctggaagcc cgcgatcggg cgatcgaact gctccctggt ctgcgcgtat tccagtgcgg tacgcaagca ttcacgggcg gcgcccatcg agccgaagat gatgccaaaa cgcgcctcgt tgaggcacga cagcggggcc ttgagcccga cggctaatgg cagcctgttc ttctccggca cccacacatc aacgaggttg agctcggcgg tcacggatgc gcgcaaagac atcttcgaat ggatggtctg ggcatcgaac cccgccgaat cggtcgacac gagaaaacca cggacgccct cgtcggtctg cgcccagatg accgcgacat cggcgatcga cccgttggta atccacatct tggtcccatt gagtacccag tcgctgccat cccggcgcgc gtgcgtgcgc atgccgcccg ggttggaccc gaaatcgggc tcggtcagac cgaaacaccc cagctcttcg cccgttgcca tcctgggcag ccacttctgc ttctgcgcat cactgccata ggcatagatg gcatacatcg ccaacgaccc ctgcacactg accaacgagc ggatccccga gtcaccggcc tccagctcca tgcaggccag tccatatgcg agagaggacg ttccggaaca tccgtatccg tgcagatgca tcccgagtac accgatcttg cccagctcca gcgcgagctt gcgtaccggc agatcaccat gctcgtacca gcgctgaaca ttgggtacca cgcgcgcctg gaccagcgcg cgcacgctgt cacggatcgc gcgctcctcg tcgctgagca gcgcatcgat accgaacaac tcgtcggggt tggttggtgc agcgtcggta gccatcgaac caggaccctg ggtctgagct tcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMATDAAPTN PDELFGIDAL LSDEERAIRD SVRALVQARV VPNVQRWYEH GDLPVRKLAL ELGKIGVLGM HLHGYGCSGT SSLAYGLACM ELEAGDSGIR SLVSVQGSLA MYAIYAYGSD AQKQKWLPRM ATGEELGCFG LTEPDFGSNP GGMRTHARRD GSDWVLNGTK MWITNGSIAD VAVIWAQTDE GVRGFLVSTD SAGFDAQTIH SKMSLRASVT AELNLVDVWV PEKNRLPLAV GLKAPLSCLN EARFGIIFGS MGAARECLRT ALEYAQTREQ FDRPIAGFQL TQEKLANMAL EYGKGVLLAL HLGRRKDADT LATPQVSLGK LNNVREAIEI ARTARTILGA NGISGEYPVM RHANNLESVL TYEGTSEMHT LVIGQALTGI AAFR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaccgac gctgcaccaa ccaaccccga cgagttgttc ggtatcgatg cgctgctcag cgacgaggag cgcgcgatcc gtgacagcgt gcgcgcgctg gtccaggcgc gcgtggtacc caatgttcag cgctggtacg agcatggtga tctgccggta cgcaagctcg cgctggagct gggcaagatc ggtgtactcg ggatgcatct gcacggatac ggatgttccg gaacgtcctc tctcgcatat ggactggcct gcatggagct ggaggccggt gactcgggga tccgctcgtt ggtcagtgtg caggggtcgt tggcgatgta tgccatctat gcctatggca gtgatgcgca gaagcagaag tggctgccca ggatggcaac gggcgaagag ctggggtgtt tcggtctgac cgagcccgat ttcgggtcca acccgggcgg catgcgcacg cacgcgcgcc gggatggcag cgactgggta ctcaatggga ccaagatgtg gattaccaac gggtcgatcg ccgatgtcgc ggtcatctgg gcgcagaccg acgagggcgt ccgtggtttt ctcgtgtcga ccgattcggc ggggttcgat gcccagacca tccattcgaa gatgtctttg cgcgcatccg tgaccgccga gctcaacctc gttgatgtgt gggtgccgga gaagaacagg ctgccattag ccgtcgggct caaggccccg ctgtcgtgcc tcaacgaggc gcgttttggc atcatcttcg gctcgatggg cgccgcccgt gaatgcttgc gtaccgcact ggaatacgcg cagaccaggg agcagttcga tcgcccgatc gcgggcttcc agctgaccca agagaaactg gccaacatgg cgctggaata cggcaaggga gtgctactgg cgctgcatct ggggcgccgc aaggatgccg acactctggc gaccccgcag gtcagcctgg gcaagctcaa caatgtgcgc gaggccatcg agatagcccg gaccgcccgc acaatccttg gtgcgaacgg tatttcgggc gagtacccgg tgatgaggca tgccaacaac ctggaatcgg tgctgaccta cgagggcacc agcgagatgc acacactggt tatcggccag gcgctgaccg ggattgcggc gttccgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa