SSGCID: Target Page

MysmA.01640.a

Glutaryl-CoA dehydrogenase

CENTER ID:	MysmA.01640.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01640.a.A1.PS00679	Full length( MysmA.01640.a )		1	396

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5137
RefSeq:	YP_889512.1
UniProt:	A0R2X4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAPSNAAAGT TAVADLLDTD SMLNPEELEL RSTVRRFGEQ RLRPFVAEWF ESGSVPVREL ATEFGKLGLL GMHLEGYGCG GSSATAYGVA CQELEAVDSG LRSMVSVQGS LAMFAIHAHG SEEQREQWLP RMATGEVIGC FGLTEPDFGS NPSGMRTTAR RDGSDWILNG SKMWITNGSV ADVAIVWARS EDGILGFVVP TDTPGFVGRE MKHKLSLRAS NTSELHLDDV RLPAEAQLPN ARGLSGPLAC LSEARFGIVF GSVGAARDCL EATLDYVGTR EVFDKTLASY QITQTKIADM AVELSKAQLL ALHLGRLKDA GRISPEQVSV GKLNNVREAL EIARQCRTLL GANGITLEYP VLRHANNLES VLTYEGTSEV HQMVIAQALT GVSAFR

NT Sequence

ATGGCTCCAT CCAACGCGGC GGCCGGGACG ACGGCGGTCG CCGACCTGCT CGACACCGAT TCGATGCTCA ACCCCGAGGA ACTGGAGCTG CGCAGCACCG TACGCCGGTT CGGCGAGCAA CGCCTGCGGC CATTTGTCGC AGAGTGGTTC GAATCCGGTT CGGTGCCGGT GCGCGAACTG GCCACCGAGT TCGGCAAGCT GGGCCTGCTC GGCATGCACC TGGAGGGTTA CGGCTGCGGC GGCTCGTCGG CCACGGCGTA CGGCGTCGCC TGCCAGGAAC TCGAGGCCGT GGACAGCGGC CTGCGCAGCA TGGTGTCGGT GCAGGGATCG CTGGCCATGT TCGCCATCCA CGCGCACGGC AGCGAGGAGC AGCGCGAGCA GTGGCTGCCG CGAATGGCCA CGGGCGAGGT GATCGGCTGC TTCGGGCTCA CCGAACCGGA CTTCGGCTCC AACCCGTCGG GTATGCGCAC GACCGCGCGG CGCGACGGCT CCGACTGGAT CCTCAACGGT TCGAAGATGT GGATCACCAA CGGCTCGGTG GCCGACGTCG CGATCGTGTG GGCGCGGTCC GAGGACGGCA TCCTGGGGTT CGTGGTGCCG ACGGACACCC CGGGCTTCGT CGGCCGCGAG ATGAAGCACA AGCTGTCGCT GCGGGCGTCG AACACCTCCG AGTTGCATCT CGACGATGTG CGTCTGCCCG CCGAGGCACA GTTGCCGAAC GCCAGAGGGC TGTCGGGCCC GTTGGCGTGC CTCTCCGAGG CGCGTTTCGG CATCGTGTTC GGTTCGGTCG GCGCGGCGCG CGACTGCCTG GAGGCAACGC TGGACTACGT CGGCACCCGC GAGGTGTTCG ACAAGACACT CGCGTCCTAC CAGATCACGC AGACCAAGAT CGCCGACATG GCAGTCGAAC TCAGCAAGGC GCAGCTACTG GCGCTGCATC TGGGCCGGCT CAAGGACGCG GGCAGGATCT CCCCCGAGCA GGTCAGCGTC GGCAAGCTCA ACAATGTCCG CGAGGCTCTC GAGATCGCCA GGCAGTGCCG AACCCTGTTG GGCGCCAACG GGATCACGCT GGAGTACCCG GTGCTGCGGC ACGCCAACAA CCTGGAGTCG GTGCTGACCT ACGAGGGCAC CTCCGAGGTG CACCAGATGG TCATCGCCCA GGCGCTGACC GGTGTCAGCG CATTTCGT

Details for MysmA.01640.a.A1.PS00679

PURIFICATION DATe:	7/15/2010
CONCENTRATION:	59.41mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	22
PERCENT COVERAGE:	74

Protocol Notes

notes unavailable

Validated AA Sequence

MVSVQGSLXX VRHPRARQRG AARAVAAANG HGRGDRLLRA HRTGLRLQPV GYAHDRAARR LRLDPQRFED VDHQRLGGRR RDRVGAVRGR HPGVRGADGH PGLRRPRDEA QAVAAGVEHL RVASRRCASA RRGTVAERQR AVGPVGVPLR GAFRHRVRFG RRGARLPGGN AGLRRHPRGV RQDTRVLPDH ADQDRRHGSR TQQGAATGAA SGPAQGRGQD LPRAGQRRQA QQCPRGSRDR QAVPNPVGRQ RDHAGVPGAA ARQQPGVGAD LRGHLRGAPD GHRPGADRCQ RISLINSTNK FCSQASRGSG C

Validated NT Sequence

cnttcgngct ttgttagcag ccggatcctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattaa cgaaatgcgc tgacaccggt cagcgcctgg gcgatgacca tctggtgcac ctcggaggtg ccctcgtagg tcagcaccga ctccaggttg ttggcgtgcc gcagcaccgg gtactccagc gtgatcccgt tggcgcccaa cagggttcgg cactgcctgg cgatctcgag agcctcgcgg acattgttga gcttgccgac gctgacctgc tcgggggaga tcctgcccgc gtccttgagc cggcccagat gcagcgccag tagctgcgcc ttgctgagtt cgactgccat gtcggcgatc ttggtctgcg tgatctggta ggacgcgagt gtcttgtcga acacctcgcg ggtgccgacg tagtccagcg ttgcctccag gcagtcgcgc gccgcgccga ccgaaccgaa cacgatgccg aaacgcgcct cggagaggca cgccaacggg cccgacagcc ctctggcgtt cggcaactgt gcctcggcgg gcagacgcac atcgtcgaga tgcaactcgg aggtgttcga cgcccgcagc gacagcttgt gcttcatctc gcggccgacg aagcccgggg tgtccgtcgg caccacgaac cccaggatgc cgtcctcgga ccgcgcccac acgatcgcga cgtcggccac cgagccgttg gtgatccaca tcttcgaacc gttgaggatc cagtcggagc cgtcgcgccg cgcggtcgtg cgcatacccg acgggttgga gccgaagtcc ggttcggtga gcccgaagca gccgatcacc tcgcccgtgg ccattcgcgg cagccactgc tcgcgctgct cctcgctgcc gtgcgcgtgg atggcgaaca tngncnagcg atccctgcac cgacaccatg ctgcgnaggc cgctgtccac nggcctcgag ttcctggagg cgacgccgta cgccgtnnnn acgagccncn ncanccgtan ccntncgnng catgncgagc angnccagct tgncnannng gnnnnnnnnn ngcancngna ccnannngnn tnnnnnnnnn gnnnnnaang nnnnnnngnn nnannnnnnn ncggnnnnnn nnnannnnnn nnnnnnnngn nnnnnnnnnn nnnnnnnngn nnnnnnnnnn nnccnnnnna

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAPSNAAAG TTAVADLLDT DSMLNPEELE LRSTVRRFGE QRLRPFVAEW FESGSVPVRE LATEFGKLGL LGMHLEGYGC GGSSATAYGV ACQELEAVDS GLRSMVSVQG SLAMFAIHAH GSEEQREQWL PRMATGEVIG CFGLTEPDFG SNPSGMRTTA RRDGSDWILN GSKMWITNGS VADVAIVWAR SEDGILGFVV PTDTPGFVGR EMKHKLSLRA SNTSELHLDD VRLPAEAQLP NARGLSGPLA CLSEARFGIV FGSVGAARDC LEATLDYVGT REVFDKTLAS YQITQTKIAD MAVELSKAQL LALHLGRLKD AGRISPEQVS VGKLNNVREA LEIARQCRTL LGANGITLEY PVLRHANNLE SVLTYEGTSE VHQMVIAQAL TGVSAFR

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCCATCC AACGCGGCGG CCGGGACGAC GGCGGTCGCC GACCTGCTCG ACACCGATTC GATGCTCAAC CCCGAGGAAC TGGAGCTGCG CAGCACCGTA CGCCGGTTCG GCGAGCAACG CCTGCGGCCA TTTGTCGCAG AGTGGTTCGA ATCCGGTTCG GTGCCGGTGC GCGAACTGGC CACCGAGTTC GGCAAGCTGG GCCTGCTCGG CATGCACCTG GAGGGTTACG GCTGCGGCGG CTCGTCGGCC ACGGCGTACG GCGTCGCCTG CCAGGAACTC GAGGCCGTGG ACAGCGGCCT GCGCAGCATG GTGTCGGTGC AGGGATCGCT GGCCATGTTC GCCATCCACG CGCACGGCAG CGAGGAGCAG CGCGAGCAGT GGCTGCCGCG AATGGCCACG GGCGAGGTGA TCGGCTGCTT CGGGCTCACC GAACCGGACT TCGGCTCCAA CCCGTCGGGT ATGCGCACGA CCGCGCGGCG CGACGGCTCC GACTGGATCC TCAACGGTTC GAAGATGTGG ATCACCAACG GCTCGGTGGC CGACGTCGCG ATCGTGTGGG CGCGGTCCGA GGACGGCATC CTGGGGTTCG TGGTGCCGAC GGACACCCCG GGCTTCGTCG GCCGCGAGAT GAAGCACAAG CTGTCGCTGC GGGCGTCGAA CACCTCCGAG TTGCATCTCG ACGATGTGCG TCTGCCCGCC GAGGCACAGT TGCCGAACGC CAGAGGGCTG TCGGGCCCGT TGGCGTGCCT CTCCGAGGCG CGTTTCGGCA TCGTGTTCGG TTCGGTCGGC GCGGCGCGCG ACTGCCTGGA GGCAACGCTG GACTACGTCG GCACCCGCGA GGTGTTCGAC AAGACACTCG CGTCCTACCA GATCACGCAG ACCAAGATCG CCGACATGGC AGTCGAACTC AGCAAGGCGC AGCTACTGGC GCTGCATCTG GGCCGGCTCA AGGACGCGGG CAGGATCTCC CCCGAGCAGG TCAGCGTCGG CAAGCTCAAC AATGTCCGCG AGGCTCTCGA GATCGCCAGG CAGTGCCGAA CCCTGTTGGG CGCCAACGGG ATCACGCTGG AGTACCCGGT GCTGCGGCAC GCCAACAACC TGGAGTCGGT GCTGACCTAC GAGGGCACCT CCGAGGTGCA CCAGATGGTC ATCGCCCAGG CGCTGACCGG TGTCAGCGCA TTTCGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA