SSGCID: Target Page

MyabA.01640.a

Probable acyl-CoA dehydrogenase

CENTER ID:	MyabA.01640.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01640.a.A1.GE30537	Full length( MyabA.01640.a )		1	397

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01640.a.A1.PS00906	Full length( MyabA.01640.a )		1	397

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.860
RefSeq:	YP_001701570.1
UniProt:	B1MI94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTAVTPSPE MCSTPLELFD TDRRLDQDER DIAATVRKFV DTKLRPNIAE WFESATLPRD LARQFGELGV LGMHLDGYGC AGTNAVSYGL ACLELEAGDS GLRSFVSVQG SLSMYSIHRY GSEEQKQEWL PQLASGHAIG CFGLTEPDFG SNPGGMRTRA RRDGKDWILD GTKMWITNGN LADVATVWAQ TDDGVRGFVV PTATKGFAAN VIHKKLSLRA SVTSELVLDG VRLPDSAELP GARGLSAPLS CLNEARFGIV FGALGAARDS LQAALDYTRT REVFGRPLSS YQLSQEKLAN MTVQLGMGML LALHLGRIKD SVGLRPEQVS LGKLNNVREA LNIARECRTL LGASGITLEY SPLRHANNLE SVLTYEGTSE MHLLSIGRAL TGQAAFR

NT Sequence

ATGTCCACTG CAGTAACCCC GTCGCCGGAG ATGTGCTCGA CCCCGCTGGA GCTGTTCGAC ACAGATCGCC GGCTGGACCA GGACGAGCGC GACATCGCGG CCACGGTGCG TAAGTTCGTC GATACCAAGC TGCGTCCGAA CATCGCGGAA TGGTTTGAGT CCGCGACACT TCCCAGAGAT CTGGCACGCC AGTTCGGTGA ACTGGGTGTG CTCGGTATGC ACCTCGACGG CTACGGGTGT GCGGGCACCA ACGCCGTGAG CTACGGGTTG GCTTGCCTTG AATTGGAGGC AGGCGATAGC GGTCTGCGCA GCTTCGTGTC GGTGCAGGGT TCTCTGTCCA TGTATTCGAT CCACCGGTAC GGCTCGGAAG AGCAGAAGCA GGAATGGTTG CCGCAGTTGG CATCTGGTCA TGCCATTGGC TGCTTCGGTC TTACCGAGCC GGACTTCGGA TCCAACCCCG GGGGGATGCG AACCCGCGCA CGCCGCGACG GCAAGGACTG GATACTCGAC GGCACGAAGA TGTGGATCAC CAACGGCAAT CTTGCCGATG TCGCCACAGT CTGGGCGCAG ACCGACGACG GTGTGCGTGG TTTCGTCGTG CCGACGGCCA CCAAGGGATT CGCCGCCAAT GTGATTCACA AGAAGCTATC CCTGCGGGCA TCGGTGACCT CGGAGCTTGT GCTCGACGGT GTGCGGCTGC CGGATTCGGC GGAACTGCCC GGCGCCCGGG GGCTTTCGGC TCCGCTGTCC TGTCTCAACG AAGCACGTTT CGGCATCGTC TTCGGCGCGC TGGGCGCCGC GCGCGACAGC CTGCAAGCGG CGCTTGACTA TACGCGTACT CGCGAGGTGT TCGGGCGTCC GTTGTCGAGC TATCAGCTCA GCCAGGAGAA GCTCGCCAAC ATGACGGTTC AGCTGGGCAT GGGCATGCTG TTGGCGCTGC ATCTGGGCCG CATCAAGGAC TCTGTCGGGT TACGGCCGGA GCAGGTCAGC CTGGGCAAGC TGAACAACGT GCGTGAGGCA CTCAACATCG CCCGTGAATG CCGAACCCTA TTAGGTGCAA GCGGTATCAC CCTTGAATAC TCGCCCCTGC GCCATGCCAA CAATCTTGAA TCGGTGCTCA CCTATGAGGG CACGTCCGAG ATGCATCTGC TTTCGATCGG GCGAGCGCTG ACCGGCCAGG CAGCCTTCCG C

Details for MyabA.01640.a.A1.GE30537

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	77

Validated AA Sequence

MHLDGYGCAG TNAVSYGLAC LELEAGDSGL RSFVSVQGSL SMYSIHRYGS EEQKQEWLPQ LASGHAIGCF GLTEPDFGSN PGGMRTRARR DGKDWILDGT KMWITNGNLA DVATVWAQTD DGVRGFVVPT ATKGFAANVI HKKLSLRASV TSELVLDGVR LPDSAELPGA RGLSAPLSCL NEARFGIVFG ALGAARDSLQ AALDYTRTRE VFGRPLSSYQ LSQEKLANMT VQLGMGMLLA LHLGRIKDSV GLRPEQVSLG KLNNVREALN IARECRTLLG ASGITLEYSP LRHANNLESV LTYEGTSEMH LLSIGRALTG QAAFR

Validated NT Sequence

ttattagcgg aaggctgcct ggccggtcag cgctcgcccg atcgaaagca gatgcatctc ggacgtgccc tcataggtga gcaccgattc aagattgttg gcatggcgca ggggcgagta ttcaagggtg ataccgcttg cacctaatag ggttcggcat tcacgggcga tgttgagtgc ctcacgcacg ttgttcagct tgcccaggct gacctgctcc ggccgtaacc cgacagagtc cttgatgcgg cccagatgca gcgccaacag catgcccatg cccagctgaa ccgtcatgtt ggcgagcttc tcctggctga gctgatagct cgacaacgga cgcccgaaca cctcgcgagt acgcgtatag tcaagcgccg cttgcaggct gtcgcgcgcg gcgcccagcg cgccgaagac gatgccgaaa cgtgcttcgt tgagacagga cagcggagcc gaaagccccc gggcgccggg cagttccgcc gaatccggca gccgcacacc gtcgagcaca agctccgagg tcaccgatgc ccgcagggat agcttcttgt gaatcacatt ggcggcgaat cccttggtgg ccgtcggcac gacgaaacca cgcacaccgt cgtcggtctg cgcccagact gtggcgacat cggcaagatt gccgttggtg atccacatct tcgtgccgtc gagtatccag tccttgccgt cgcggcgtgc gcgggttcgc atccccccgg ggttggatcc gaagtccggc tcggtaagac cgaagcagcc aatggcatga ccagatgcca actgcggcaa ccattcctgc ttctgctctt ccgagccgta ccggtggatc gaatacatgg acagagaacc ctgcaccgac acgaagctgc gcagaccgct atcgcctgcc tccaattcaa ggcaagccaa cccgtagctc acggcgttgg tgcccgcaca cccgtagccg tcgaggtgca taccgagcac acccagttca ccgaactggc gtgccagatc tctgggaagt gtcgcggact caaaccattc cgcgatgttc ggacgcagct tggtatcgac gaacttacgc accgtggccg cgatgtcgcg ctcgtcctgg tccagccggc gat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTAVTPSP EMCSTPLELF DTDRRLDQDE RDIAATVRKF VDTKLRPNIA EWFESATLPR DLARQFGELG VLGMHLDGYG CAGTNAVSYG LACLELEAGD SGLRSFVSVQ GSLSMYSIHR YGSEEQKQEW LPQLASGHAI GCFGLTEPDF GSNPGGMRTR ARRDGKDWIL DGTKMWITNG NLADVATVWA QTDDGVRGFV VPTATKGFAA NVIHKKLSLR ASVTSELVLD GVRLPDSAEL PGARGLSAPL SCLNEARFGI VFGALGAARD SLQAALDYTR TREVFGRPLS SYQLSQEKLA NMTVQLGMGM LLALHLGRIK DSVGLRPEQV SLGKLNNVRE ALNIARECRT LLGASGITLE YSPLRHANNL ESVLTYEGTS EMHLLSIGRA LTGQAAFR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccactgca gtaaccccgt cgccggagat gtgctcgacc ccgctggagc tgttcgacac agatcgccgg ctggaccagg acgagcgcga catcgcggcc acggtgcgta agttcgtcga taccaagctg cgtccgaaca tcgcggaatg gtttgagtcc gcgacacttc ccagagatct ggcacgccag ttcggtgaac tgggtgtgct cggtatgcac ctcgacggct acgggtgtgc gggcaccaac gccgtgagct acgggttggc ttgccttgaa ttggaggcag gcgatagcgg tctgcgcagc ttcgtgtcgg tgcagggttc tctgtccatg tattcgatcc accggtacgg ctcggaagag cagaagcagg aatggttgcc gcagttggca tctggtcatg ccattggctg cttcggtctt accgagccgg acttcggatc caaccccggg gggatgcgaa cccgcgcacg ccgcgacggc aaggactgga tactcgacgg cacgaagatg tggatcacca acggcaatct tgccgatgtc gccacagtct gggcgcagac cgacgacggt gtgcgtggtt tcgtcgtgcc gacggccacc aagggattcg ccgccaatgt gattcacaag aagctatccc tgcgggcatc ggtgacctcg gagcttgtgc tcgacggtgt gcggctgccg gattcggcgg aactgcccgg cgcccggggg ctttcggctc cgctgtcctg tctcaacgaa gcacgtttcg gcatcgtctt cggcgcgctg ggcgccgcgc gcgacagcct gcaagcggcg cttgactata cgcgtactcg cgaggtgttc gggcgtccgt tgtcgagcta tcagctcagc caggagaagc tcgccaacat gacggttcag ctgggcatgg gcatgctgtt ggcgctgcat ctgggccgca tcaaggactc tgtcgggtta cggccggagc aggtcagcct gggcaagctg aacaacgtgc gtgaggcact caacatcgcc cgtgaatgcc gaaccctatt aggtgcaagc ggtatcaccc ttgaatactc gcccctgcgc catgccaaca atcttgaatc ggtgctcacc tatgagggca cgtccgagat gcatctgctt tcgatcgggc gagcgctgac cggccaggca gccttccgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for MyabA.01640.a.A1.PS00906

PURIFICATION DATe:	12/14/2010
CONCENTRATION:	27.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	77

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCACTGCA GTAACCCCGT CGCCGGAGAT GTGCTCGACC CCGCTGGAGC TGTTCGACAC AGATCGCCGG CTGGACCAGG ACGAGCGCGA CATCGCGGCC ACGGTGCGTA AGTTCGTCGA TACCAAGCTG CGTCCGAACA TCGCGGAATG GTTTGAGTCC GCGACACTTC CCAGAGATCT GGCACGCCAG TTCGGTGAAC TGGGTGTGCT CGGTATGCAC CTCGACGGCT ACGGGTGTGC GGGCACCAAC GCCGTGAGCT ACGGGTTGGC TTGCCTTGAA TTGGAGGCAG GCGATAGCGG TCTGCGCAGC TTCGTGTCGG TGCAGGGTTC TCTGTCCATG TATTCGATCC ACCGGTACGG CTCGGAAGAG CAGAAGCAGG AATGGTTGCC GCAGTTGGCA TCTGGTCATG CCATTGGCTG CTTCGGTCTT ACCGAGCCGG ACTTCGGATC CAACCCCGGG GGGATGCGAA CCCGCGCACG CCGCGACGGC AAGGACTGGA TACTCGACGG CACGAAGATG TGGATCACCA ACGGCAATCT TGCCGATGTC GCCACAGTCT GGGCGCAGAC CGACGACGGT GTGCGTGGTT TCGTCGTGCC GACGGCCACC AAGGGATTCG CCGCCAATGT GATTCACAAG AAGCTATCCC TGCGGGCATC GGTGACCTCG GAGCTTGTGC TCGACGGTGT GCGGCTGCCG GATTCGGCGG AACTGCCCGG CGCCCGGGGG CTTTCGGCTC CGCTGTCCTG TCTCAACGAA GCACGTTTCG GCATCGTCTT CGGCGCGCTG GGCGCCGCGC GCGACAGCCT GCAAGCGGCG CTTGACTATA CGCGTACTCG CGAGGTGTTC GGGCGTCCGT TGTCGAGCTA TCAGCTCAGC CAGGAGAAGC TCGCCAACAT GACGGTTCAG CTGGGCATGG GCATGCTGTT GGCGCTGCAT CTGGGCCGCA TCAAGGACTC TGTCGGGTTA CGGCCGGAGC AGGTCAGCCT GGGCAAGCTG AACAACGTGC GTGAGGCACT CAACATCGCC CGTGAATGCC GAACCCTATT AGGTGCAAGC GGTATCACCC TTGAATACTC GCCCCTGCGC CATGCCAACA ATCTTGAATC GGTGCTCACC TATGAGGGCA CGTCCGAGAT GCATCTGCTT TCGATCGGGC GAGCGCTGAC CGGCCAGGCA GCCTTCCGCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA