MysmA.01640.b

Glutaryl-CoA dehydrogenase

CENTER ID: MysmA.01640.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.01640.b.A1.PW28880 Full length( MysmA.01640.b ) 1 397

Structures

3SWO
DEPOSITED: 7/14/2011
DETERMINATION: XRay
CLONE: MysmA.01640.b.A1.GE28975
PROTEIN: MysmA.01640.b.A1.PW28880

Publications by SSGCID

Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_129299
PATRIC ID: fig|246196.19.peg.6509
RefSeq: YP_890894.1
UniProt: A0R6V6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLTAPSKKS TYAPLELFDT DRLLDQDERD IAATVRQFVD TRLKPNVEGW FESATLPSEL AKEFGNLGVL GMHLQGYGCA GTNAVSYGLA CMELEAGDSG FRSFVSVQGS LSMFSIYRYG SEEQKNEWLP RLAAGDAIGC FGLTEPDFGS NPAGMRTRAR RDGSDWILNG TKMWITNGNL ADVATVWAQT DDGIRGFLVP TDTPGFTANE IHRKLSLRAS VTSELVLDNV RLPASAQLPL AEGLSAPLSC LNEARFGIVF GALGAARDSL ETTIAYTQSR EVFDKPLSNY QLTQEKLANM TVELGKGMLL AIHLGRIKDA EGVRPEQISL GKLNNVREAI AIARECRTLL GGSGITLEYS PLRHANNLES VLTYEGTSEM HLLSIGKALT GKAAFRS
NT Sequence
ATGACCCTCA CCGCACCCTC GAAGAAGTCC ACCTACGCCC CGCTCGAGCT GTTCGACACC GACCGCCTGC TCGACCAGGA CGAGCGCGAC ATCGCCGCAA CCGTGCGGCA GTTCGTCGAC ACCCGGCTGA AGCCCAATGT CGAGGGCTGG TTCGAATCGG CCACTCTCCC AAGCGAACTC GCCAAGGAGT TCGGCAATCT CGGTGTGCTG GGCATGCACC TGCAGGGTTA CGGCTGCGCG GGCACCAACG CCGTGAGCTA CGGCCTGGCC TGCATGGAAC TCGAGGCAGG CGACAGCGGC TTCCGCAGCT TCGTGTCGGT GCAGGGCTCG CTGTCGATGT TCTCGATCTA CCGCTACGGC TCGGAAGAGC AGAAGAACGA GTGGCTGCCC CGCCTGGCCG CGGGTGACGC CATCGGCTGT TTCGGTCTGA CCGAACCCGA CTTCGGCTCC AACCCCGCCG GTATGCGCAC CCGCGCGCGC CGCGACGGCA GCGACTGGAT CCTCAACGGC ACCAAGATGT GGATCACCAA CGGCAACCTG GCCGACGTCG CCACGGTGTG GGCACAGACC GACGACGGCA TCCGCGGTTT CCTGGTCCCC ACCGACACAC CGGGATTCAC CGCCAACGAG ATCCACCGCA AGCTCTCGCT GCGCGCGTCG GTGACCTCCG AACTGGTGCT CGACAACGTG CGCCTGCCCG CGTCGGCCCA GCTGCCGCTG GCCGAGGGCC TCTCGGCTCC GCTGTCGTGC CTCAACGAGG CACGGTTCGG CATCGTGTTC GGCGCGCTGG GCGCCGCACG CGACAGCCTG GAGACCACGA TCGCCTACAC GCAGTCGCGC GAGGTGTTCG ACAAGCCGTT GTCGAATTAC CAGCTGACGC AGGAGAAGCT GGCCAACATG ACCGTCGAGC TGGGCAAGGG CATGTTGCTG GCCATCCACC TGGGCCGCAT CAAAGACGCC GAGGGTGTGC GCCCCGAGCA GATCAGCCTG GGCAAGCTCA ACAACGTCCG CGAGGCCATC GCGATCGCCC GCGAATGCCG GACCCTGTTG GGCGGCAGCG GGATCACGCT CGAGTACTCG CCGCTGCGTC ACGCCAACAA CCTGGAGTCG GTGCTCACCT ACGAGGGCAC CTCCGAGATG CATCTGCTGT CCATCGGCAA GGCGCTCACC GGAAAGGCGG CGTTCCGTTC A
Details for MysmA.01640.b.A1.PW28880
PURIFICATION DATe: 3/25/2011
CONCENTRATION: 30.64mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 19
PERCENT COVERAGE: 83
Protocol Notes
notes unavailable
Validated AA Sequence
MHLGGALVGE HRLQVVGVTQ RRVLERDPAA AQQGPAFAGD RDGLADVVEL AQADLLGAHT LGVFDAAQVD GQQHALAQLD GHVGQLLLRQ LVIRQRLVEH LARLRVGDRG LQAVACGAQR AEHDAEPCLV EARQRSREAL GQRQLGRRGQ AHVVEHQFGG HRRAQRELAV DLVGGESRCV GGDQETADAV VGLCPHRGDV GQVAVGDPHL GAVEDPVAAV AARAGAHTGG VGAEVGFGQT ETADGVTRGQ AGQPLVLLLF RAVAVDREHR QRALHRXEXA XAAVAXLXLC XPXVXXGVXP AXRXPXHXXT XDXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
cgcggatcct cnagaagctt ggctgcagaa cttgttcgtg ctgtttatta tgaacggaac gccgcctttc cggtgagcgc cttgccgatg gacagcagat gcatctcgga ggtgccctcg taggtgagca ccgactccag gttgttggcg tgacgcagcg gcgagtactc gagcgtgatc ccgctgccgc ccaacagggt ccggcattcg cgggcgatcg cgatggcctc gcggacgttg ttgagcttgc ccaggctgat ctgctcgggg cgcacaccct cggcgtcttt gatgcggccc aggtggatgg ccagcaacat gcccttgccc agctcgacgg tcatgttggc cagcttctcc tgcgtcagct ggtaattcga caacggcttg tcgaacacct cgcgcgactg cgtgtaggcg atcgtggtct ccaggctgtc gcgtgcggcg cccagcgcgc cgaacacgat gccgaaccgt gcctcgttga ggcacgacag cggagccgag aggccctcgg ccagcggcag ctgggccgac gcgggcaggc gcacgttgtc gagcaccagt tcggaggtca ccgacgcgcg cagcgagagc ttgcggtgga tctcgttggc ggtgaatccc ggtgtgtcgg tggggaccag gaaaccgcgg atgccgtcgt cggtctgtgc ccacaccgtg gcgacgtcgg ccaggttgcc gttggtgatc cacatcttgg tgccgttgag gatccagtcg ctgccgtcgc ggcgcgcgcg ggtgcgcata ccggcggggt tggagccgaa gtcgggttcg gtcagaccga aacagccgat ggcgtcaccc gcggccaggc ggggcagcca ctcgttcttc tgctcttccg agccgtagcg gtagatcgag aacatcgaca gcgagccctg caccgacncg aanctgcnnn agccgctgtc gcctgnctcg nnttatgcng gccnnnngta nctnanggcg tnnngccngc gcancgtanc ccnnngcatg ncngnacnnn agatnnnant ncnnnnnnnn nnntggnnnn natnnannnn nnnnnacntn gnnnnnnnnn nnnnnncnan nnncnnnnnn nnnnnannnn nnnnnnnnnn nnnnannnnn nnnnnnnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLTAPSKK STYAPLELFD TDRLLDQDER DIAATVRQFV DTRLKPNVEG WFESATLPSE LAKEFGNLGV LGMHLQGYGC AGTNAVSYGL ACMELEAGDS GFRSFVSVQG SLSMFSIYRY GSEEQKNEWL PRLAAGDAIG CFGLTEPDFG SNPAGMRTRA RRDGSDWILN GTKMWITNGN LADVATVWAQ TDDGIRGFLV PTDTPGFTAN EIHRKLSLRA SVTSELVLDN VRLPASAQLP LAEGLSAPLS CLNEARFGIV FGALGAARDS LETTIAYTQS REVFDKPLSN YQLTQEKLAN MTVELGKGML LAIHLGRIKD AEGVRPEQIS LGKLNNVREA IAIARECRTL LGGSGITLEY SPLRHANNLE SVLTYEGTSE MHLLSIGKAL TGKAAFRS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCTCACC GCACCCTCGA AGAAGTCCAC CTACGCCCCG CTCGAGCTGT TCGACACCGA CCGCCTGCTC GACCAGGACG AGCGCGACAT CGCCGCAACC GTGCGGCAGT TCGTCGACAC CCGGCTGAAG CCCAATGTCG AGGGCTGGTT CGAATCGGCC ACTCTCCCAA GCGAACTCGC CAAGGAGTTC GGCAATCTCG GTGTGCTGGG CATGCACCTG CAGGGTTACG GCTGCGCGGG CACCAACGCC GTGAGCTACG GCCTGGCCTG CATGGAACTC GAGGCAGGCG ACAGCGGCTT CCGCAGCTTC GTGTCGGTGC AGGGCTCGCT GTCGATGTTC TCGATCTACC GCTACGGCTC GGAAGAGCAG AAGAACGAGT GGCTGCCCCG CCTGGCCGCG GGTGACGCCA TCGGCTGTTT CGGTCTGACC GAACCCGACT TCGGCTCCAA CCCCGCCGGT ATGCGCACCC GCGCGCGCCG CGACGGCAGC GACTGGATCC TCAACGGCAC CAAGATGTGG ATCACCAACG GCAACCTGGC CGACGTCGCC ACGGTGTGGG CACAGACCGA CGACGGCATC CGCGGTTTCC TGGTCCCCAC CGACACACCG GGATTCACCG CCAACGAGAT CCACCGCAAG CTCTCGCTGC GCGCGTCGGT GACCTCCGAA CTGGTGCTCG ACAACGTGCG CCTGCCCGCG TCGGCCCAGC TGCCGCTGGC CGAGGGCCTC TCGGCTCCGC TGTCGTGCCT CAACGAGGCA CGGTTCGGCA TCGTGTTCGG CGCGCTGGGC GCCGCACGCG ACAGCCTGGA GACCACGATC GCCTACACGC AGTCGCGCGA GGTGTTCGAC AAGCCGTTGT CGAATTACCA GCTGACGCAG GAGAAGCTGG CCAACATGAC CGTCGAGCTG GGCAAGGGCA TGTTGCTGGC CATCCACCTG GGCCGCATCA AAGACGCCGA GGGTGTGCGC CCCGAGCAGA TCAGCCTGGG CAAGCTCAAC AACGTCCGCG AGGCCATCGC GATCGCCCGC GAATGCCGGA CCCTGTTGGG CGGCAGCGGG ATCACGCTCG AGTACTCGCC GCTGCGTCAC GCCAACAACC TGGAGTCGGT GCTCACCTAC GAGGGCACCT CCGAGATGCA TCTGCTGTCC ATCGGCAAGG CGCTCACCGG AAAGGCGGCG TTCCGTTCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A