MyavA.01530.b
Carnitinyl-CoA dehydratase
| CENTER ID: | MyavA.01530.b |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01530.b.A1.GE29126 | Full length( MyavA.01530.b ) | 1 | 263 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party
can then use to order the clone from a third-party clone manufacturer.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01530.b.A1.PS00726 | Full length( MyavA.01530.b ) | 1 | 263 |
Structures
3R9S
DEPOSITED: 3/25/2011
DETERMINATION: XRay
CLONE: MyavA.01530.b.A1.GE29126
PROTEIN: MyavA.01530.b.A1.PS00726
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3MOY,
3MYB,
3LLS,
3GVG,
3MPZ,
3NF4,
3ICO,
3H81,
3HWK,
3NDN,
3KHP,
3GVC,
3HWI,
3H7F,
3HE2,
3GWC,
3HZG,
3NG3,
3OI9,
3OC7,
3NDO,
3OME,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OL3,
3OL4,
3OEC,
3QXZ,
3QUV,
3PPI,
3P4I,
3Q1T,
3PXX,
3QHA,
3P5M,
3R0O,
3P85,
3QLJ,
3QH4,
3QRE,
3QMJ,
3QKA,
3QDF,
3QK8,
3R1I,
3QBP,
3QXI,
3QIV,
3P0T,
3PZY,
3PGX,
3QYR,
3P4T,
3QUA,
3P2Y,
3Q8N,
3OKS,
3PK0,
3PE8,
3TDE,
3R7K,
3SLL,
3R9Q,
3RR6,
3R6O,
3R8C,
4DIE,
4DXL,
4ED4,
4EMD,
3TCR,
3R9R,
4EYE,
3TX2,
3S55,
3RIH,
3RRP,
3SWX,
3RSI,
3TRR,
3TAV,
3RD7,
3SVK,
3R1J,
3UVE,
3T7C,
3R9S,
3S82,
3R2N,
4DI1,
3U0A,
3TZQ,
4F3W,
3SBX,
4DQ8,
3RFQ,
3R6H,
3R4T,
3TZU,
3RR2,
3SWT,
4F47,
3RV2,
3R9P,
3RRV,
3TJR,
3RD5,
3SX2,
3TSC,
3R9T,
3TLF,
3U0B,
3R20,
3UCX,
4EGF,
3RD8,
3SWO,
3SF6,
3SVT,
3TL3,
4EGE,
3T3W,
4EO9,
4HR3,
4FFC,
4GK6,
4IZ9,
4LGV,
5EJ2,
4KAM,
4O2D,
4IJN,
4J5I,
4IV6,
4I1Y,
4HDT
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|243243.7.peg.1250 |
| RefSeq: | YP_880522.1 |
| UniProt: | A0A0H2ZTH2 |
Orthologues for Rv0222
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| marinum | 92 | 97 | 1 | 1 | 1 |
| ulcerans | 91 | 97 | 0 | 0 | 0 |
| avium | 85 | 96 | 1 | 1 | 0 |
| paratuberculosis | 85 | 96 | 1 | 1 | 0 |
| abscessus | 69 | 95 | 1 | 1 | 1 |
| smegmatis | 66 | 97 | 0 | 0 | 0 |
| thermoresistibile | 54 | 57 | 0 | 0 | 0 |
| thermoresistibile | 49 | 72 | 1 | 0 | 0 |
| avium | 48 | 95 | 1 | 1 | 1 |
| paratuberculosis | 47 | 95 | 1 | 1 | 1 |
| smegmatis | 47 | 95 | 1 | 1 | 0 |
| marinum | 45 | 97 | 0 | 0 | 0 |
| avium | 45 | 95 | 1 | 1 | 1 |
| ulcerans | 44 | 97 | 0 | 0 | 0 |
| smegmatis | 43 | 95 | 0 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDAPGALAE RRGNVMVITI NRPEARNAVN AAVSIGVGDA LEEAQHDPEV RAVVLTGAGD KSFCAGADLK AIARRENLYH PDHPEWGFAG YVRHFIDKPT IAAVNGTALG GGTELALASD LVVADERAQF GLPEVKRGLI AAAGGVFRIA EQLPRKVAMR LLLTGEPLSA AAARDWGLIN EVVEAGSVLD AALALASAIT VNAPLSVQAS KRIAYGVDDG VVVGDEPGWD RTMREMRALL KSEDAKEGPR AFAEKREPVW QAR
NT Sequence
GTGACCGACG CGCCGGGGGC CCTGGCCGAA CGCCGGGGCA ACGTGATGGT GATAACCATC AACCGGCCGG AAGCCCGCAA CGCGGTCAAC GCCGCGGTCA GCATCGGCGT CGGGGATGCG CTCGAAGAGG CGCAACACGA CCCCGAGGTG CGGGCGGTGG TGCTCACCGG CGCGGGCGAC AAGTCGTTCT GCGCGGGGGC CGACCTCAAG GCCATCGCGC GGCGGGAGAA CCTGTATCAC CCCGACCATC CCGAATGGGG CTTCGCCGGC TACGTGCGTC ACTTCATCGA CAAGCCCACC ATCGCGGCGG TCAACGGCAC CGCCCTGGGC GGGGGCACCG AGCTGGCGCT GGCCAGCGAC CTGGTGGTGG CCGACGAGCG GGCCCAGTTC GGGCTGCCGG AGGTCAAGCG CGGCCTGATC GCCGCCGCCG GTGGGGTGTT CCGGATCGCC GAGCAGCTAC CCCGCAAGGT GGCGATGCGG CTGCTGCTGA CCGGCGAGCC GCTGTCCGCG GCCGCCGCCC GCGACTGGGG CCTGATCAAC GAGGTGGTCG AGGCCGGCTC GGTGCTGGAC GCCGCGCTGG CGCTGGCCTC GGCGATCACC GTGAACGCCC CGCTGTCGGT GCAGGCCAGC AAGCGGATCG CCTACGGCGT CGACGACGGC GTGGTGGTCG GCGACGAACC CGGCTGGGAC CGCACCATGC GCGAGATGCG GGCCCTGCTC AAATCGGAGG ACGCCAAGGA GGGCCCGCGG GCGTTCGCCG AGAAGCGGGA GCCGGTCTGG CAGGCGCGC
Details for MyavA.01530.b.A1.GE29126
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDAPGAL AERRGNVMVI TINRPEARNA VNAAVSIGVG DALEEAQHDP EVRAVVLTGA GDKSFCAGAD LKAIARRENL YHPDHPEWGF AGYVRHFIDK PTIAAVNGTA LGGGTELALA SDLVVADERA QFGLPEVKRG LIAAAGGVFR IAEQLPRKVA MRLLLTGEPL SAAAARDWGL INEVVEAGSV LDAALALASA ITVNAPLSVQ ASKRIAYGVD DGVVVGDEPG WDRTMREMRA LLKSEDAKEG PRAFAEKREP VWQAR
Validated NT Sequence
ttattagcgc gcctgccaga ccggctcccg cttctcggcg aacgcccgcg ggccctcctt ggcgtcctcc gatttgagca gggcccgcat ctcgcgcatg gtgcggtccc agccgggttc gtcgccgacc accacgccgt cgtcgacgcc gtaggcgatc cgcttgctgg cctgcaccga cagcggggcg ttcacggtga tcgccgaggc cagcgccagc gcggcgtcca gcaccgagcc ggcctcgacc acctcgttga tcaggcccca gtcgcgggcg gcggccgcgg acagcggctc gccggtcagc agcagccgca tcgccacctt gcggggtagc tgctcggcga tccggaacac cccaccggcg gcggcgatca ggccgcgctt gacctccggc agcccgaact gggcccgctc gtcggccacc accaggtcgc tggccagcgc cagctcggtg cccccgccca gggcggtgcc gttgaccgcc gcgatggtgg gcttgtcgat gaagtgacgc acgtagccgg cgaagcccca ttcgggatgg tcggggtgat acaggttctc ccgccgcgcg atggccttga ggtcggcccc cgcgcagaac gacttgtcgc ccgcgccggt gagcaccacc gcccgcacct cggggtcgtg ttgcgcctct tcgagcgcat ccccgacgcc gatgctgacc gcggcgttga ccgcgttgcg ggcttccggc cggttgatgg ttatcaccat cacgttgccc cggcgttcgg ccagggcccc cggcgcgtcg gtcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDAPGAL AERRGNVMVI TINRPEARNA VNAAVSIGVG DALEEAQHDP EVRAVVLTGA GDKSFCAGAD LKAIARRENL YHPDHPEWGF AGYVRHFIDK PTIAAVNGTA LGGGTELALA SDLVVADERA QFGLPEVKRG LIAAAGGVFR IAEQLPRKVA MRLLLTGEPL SAAAARDWGL INEVVEAGSV LDAALALASA ITVNAPLSVQ ASKRIAYGVD DGVVVGDEPG WDRTMREMRA LLKSEDAKEG PRAFAEKREP VWQAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgac gcgccggggg ccctggccga acgccggggc aacgtgatgg tgataaccat caaccggccg gaagcccgca acgcggtcaa cgccgcggtc agcatcggcg tcggggatgc gctcgaagag gcgcaacacg accccgaggt gcgggcggtg gtgctcaccg gcgcgggcga caagtcgttc tgcgcggggg ccgacctcaa ggccatcgcg cggcgggaga acctgtatca ccccgaccat cccgaatggg gcttcgccgg ctacgtgcgt cacttcatcg acaagcccac catcgcggcg gtcaacggca ccgccctggg cgggggcacc gagctggcgc tggccagcga cctggtggtg gccgacgagc gggcccagtt cgggctgccg gaggtcaagc gcggcctgat cgccgccgcc ggtggggtgt tccggatcgc cgagcagcta ccccgcaagg tggcgatgcg gctgctgctg accggcgagc cgctgtccgc ggccgccgcc cgcgactggg gcctgatcaa cgaggtggtc gaggccggct cggtgctgga cgccgcgctg gcgctggcct cggcgatcac cgtgaacgcc ccgctgtcgg tgcaggccag caagcggatc gcctacggcg tcgacgacgg cgtggtggtc ggcgacgaac ccggctggga ccgcaccatg cgcgagatgc gggccctgct caaatcggag gacgccaagg agggcccgcg ggcgttcgcc gagaagcggg agccggtctg gcaggcgcgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyavA.01530.b.A1.PS00726
| PURIFICATION DATe: | 8/18/2010 |
| CONCENTRATION: | 14.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDAPGAL AERRGNVMVI TINRPEARNA VNAAVSIGVG DALEEAQHDP EVRAVVLTGA GDKSFCAGAD LKAIARRENL YHPDHPEWGF AGYVRHFIDK PTIAAVNGTA LGGGTELALA SDLVVADERA QFGLPEVKRG LIAAAGGVFR IAEQLPRKVA MRLLLTGEPL SAAAARDWGL INEVVEAGSV LDAALALASA ITVNAPLSVQ ASKRIAYGVD DGVVVGDEPG WDRTMREMRA LLKSEDAKEG PRAFAEKREP VWQAR
Validated NT Sequence
ttattagcgc gcctgccaga ccggctcccg cttctcggcg aacgcccgcg ggccctcctt ggcgtcctcc gatttgagca gggcccgcat ctcgcgcatg gtgcggtccc agccgggttc gtcgccgacc accacgccgt cgtcgacgcc gtaggcgatc cgcttgctgg cctgcaccga cagcggggcg ttcacggtga tcgccgaggc cagcgccagc gcggcgtcca gcaccgagcc ggcctcgacc acctcgttga tcaggcccca gtcgcgggcg gcggccgcgg acagcggctc gccggtcagc agcagccgca tcgccacctt gcggggtagc tgctcggcga tccggaacac cccaccggcg gcggcgatca ggccgcgctt gacctccggc agcccgaact gggcccgctc gtcggccacc accaggtcgc tggccagcgc cagctcggtg cccccgccca gggcggtgcc gttgaccgcc gcgatggtgg gcttgtcgat gaagtgacgc acgtagccgg cgaagcccca ttcgggatgg tcggggtgat acaggttctc ccgccgcgcg atggccttga ggtcggcccc cgcgcagaac gacttgtcgc ccgcgccggt gagcaccacc gcccgcacct cggggtcgtg ttgcgcctct tcgagcgcat ccccgacgcc gatgctgacc gcggcgttga ccgcgttgcg ggcttccggc cggttgatgg ttatcaccat cacgttgccc cggcgttcgg ccagggcccc cggcgcgtcg gtcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDAPGAL AERRGNVMVI TINRPEARNA VNAAVSIGVG DALEEAQHDP EVRAVVLTGA GDKSFCAGAD LKAIARRENL YHPDHPEWGF AGYVRHFIDK PTIAAVNGTA LGGGTELALA SDLVVADERA QFGLPEVKRG LIAAAGGVFR IAEQLPRKVA MRLLLTGEPL SAAAARDWGL INEVVEAGSV LDAALALASA ITVNAPLSVQ ASKRIAYGVD DGVVVGDEPG WDRTMREMRA LLKSEDAKEG PRAFAEKREP VWQAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGAC GCGCCGGGGG CCCTGGCCGA ACGCCGGGGC AACGTGATGG TGATAACCAT CAACCGGCCG GAAGCCCGCA ACGCGGTCAA CGCCGCGGTC AGCATCGGCG TCGGGGATGC GCTCGAAGAG GCGCAACACG ACCCCGAGGT GCGGGCGGTG GTGCTCACCG GCGCGGGCGA CAAGTCGTTC TGCGCGGGGG CCGACCTCAA GGCCATCGCG CGGCGGGAGA ACCTGTATCA CCCCGACCAT CCCGAATGGG GCTTCGCCGG CTACGTGCGT CACTTCATCG ACAAGCCCAC CATCGCGGCG GTCAACGGCA CCGCCCTGGG CGGGGGCACC GAGCTGGCGC TGGCCAGCGA CCTGGTGGTG GCCGACGAGC GGGCCCAGTT CGGGCTGCCG GAGGTCAAGC GCGGCCTGAT CGCCGCCGCC GGTGGGGTGT TCCGGATCGC CGAGCAGCTA CCCCGCAAGG TGGCGATGCG GCTGCTGCTG ACCGGCGAGC CGCTGTCCGC GGCCGCCGCC CGCGACTGGG GCCTGATCAA CGAGGTGGTC GAGGCCGGCT CGGTGCTGGA CGCCGCGCTG GCGCTGGCCT CGGCGATCAC CGTGAACGCC CCGCTGTCGG TGCAGGCCAG CAAGCGGATC GCCTACGGCG TCGACGACGG CGTGGTGGTC GGCGACGAAC CCGGCTGGGA CCGCACCATG CGCGAGATGC GGGCCCTGCT CAAATCGGAG GACGCCAAGG AGGGCCCGCG GGCGTTCGCC GAGAAGCGGG AGCCGGTCTG GCAGGCGCGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA