MysmA.01530.a

Carnitinyl-CoA dehydratase

CENTER ID: MysmA.01530.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01530.a.A1.GE28940 Full length( MysmA.01530.a ) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01530.a.A1.PS00643 Full length( MysmA.01530.a ) 1 264

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5071
RefSeq: YP_889444.1
UniProt: A0R2Q6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTETAPGALT ERRGNVLLIT INRPEARNAI NSSVSQAVGD ALAEAQNDPE VRAVVLTGAG DKSFCAGADL KAISRGENLF HPEHPEYGFA GYVSHFIDKP TIAAVNGTAL GGGTELALAS DLVVAEERAM FGLPEVKRGL IAGAGGVFRI IEQLPRKVAL ELVYTGDPIS AADALKWGLI NQVVPDGTVV DAALALAERI TCNAPLAVQA SKRVAYGAEE GTVPAEQPSW KRTNREFVTL LKTEDAMEGP LAFAQKRQPV WKAK
NT Sequence
GTGACCGAAA CCGCGCCGGG CGCTCTCACC GAGCGCAGAG GCAATGTCCT GCTCATCACC ATCAACCGGC CCGAGGCGCG CAACGCCATC AACTCGTCGG TGAGCCAGGC CGTCGGCGAC GCGCTGGCCG AAGCGCAGAA CGACCCCGAG GTTCGCGCAG TGGTCCTCAC CGGTGCGGGT GACAAGTCGT TCTGCGCCGG TGCGGACCTC AAGGCGATCT CGCGTGGGGA GAACCTCTTC CACCCCGAGC ATCCCGAGTA CGGATTCGCA GGCTACGTCA GCCATTTCAT CGACAAGCCG ACCATCGCCG CGGTCAACGG CACCGCTCTC GGTGGCGGTA CCGAACTGGC GCTGGCCAGT GATCTGGTGG TCGCCGAGGA GCGTGCGATG TTCGGTCTGC CCGAGGTCAA GCGTGGCCTG ATCGCCGGTG CCGGAGGCGT TTTCCGCATC ATCGAGCAGC TGCCGCGCAA GGTGGCGCTG GAGTTGGTGT ACACCGGTGA CCCGATCAGC GCCGCCGACG CGCTGAAGTG GGGCCTGATC AACCAGGTGG TGCCCGACGG CACCGTCGTC GACGCGGCTC TGGCTCTCGC GGAACGCATC ACGTGCAACG CACCGCTGGC CGTGCAGGCC AGCAAGCGCG TCGCCTACGG CGCCGAGGAG GGCACGGTGC CCGCCGAGCA GCCGTCGTGG AAGCGCACGA ACCGCGAGTT CGTCACGCTG CTGAAAACCG AAGATGCCAT GGAAGGTCCG CTGGCTTTCG CGCAGAAGCG CCAGCCCGTT TGGAAGGCCA AG
Details for MysmA.01530.a.A1.GE28940
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MGXLEAQTQG PGSMVTETAP GALTERRGNV LLITINRPEA RNAINSSVSQ AVGDALAEAQ NDPEVRAVVL TGAGDKSFCA GADLKAISRG ENLFHPEHPE YGFAGYVSHF IDKPTIAAVN GTALGGGTEL ALASDLVVAE ERAMFGLPEV KRGLIAGAGG VFRIIEQLPR KVALELVYTG DPISAADALK WGLINQVVPD GTVVDAALAL AERITCNAPL AVQASKRVAY GAEEGTVPAE QPSWKRTNRE FVTLLKTEDA MEGPLAFAQK RQPVWKA
Validated NT Sequence
cttggccttc caaacgggct ggcgcttctg cgcgaaagcc agcggacctt ccatggcatc ttcggttttc agcagcgtga cgaactcgcg gttcgtgcgc ttccacgacg gctgctcggc gggcaccgtg ccctcctcgg cgccgtaggc gacgcgcttg ctggcctgca cggccagcgg tgcgttgcac gtgatgcgtt ccgcgagagc cagagccgcg tcgacgacgg tgccgtcggg caccacctgg ttgatcaggc cccacttcag cgcgtcggcg gcgctgatcg ggtcaccggt gtacaccaac tccagcgcca ccttgcgcgg cagctgctcg atgatgcgga aaacgcctcc ggcaccggcg atcaggccac gcttgacctc gggcagaccg aacatcgcac gctcctcggc gaccaccaga tcactggcca gcgccagttc ggtaccgcca ccgagagcgg tgccgttgac cgcggcgatg gtcggcttgt cgatgaaatg gctgacgtag cctgcgaatc cgtactcggg atgctcgggg tggaagaggt tctccccacg cgagatcgcc ttgaggtccg caccggcgca gaacgacttg tcacccgcac cggtgaggac cactgcgcga acctcggggt cgttctgcgc ttcggccagc gcgtcgccga cggcctggct caccgacgag ttgatggcgt tgcgcgcctc gggccggttg atggtgatga gcaggacatt gcctctgcgc tcggtgagag cgcccggcgc ggtttcngtc accatcgaac caggaccctg ggtctgagct tccagggnac ccatatgatg gtgatggtga tgagccnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTETAPGA LTERRGNVLL ITINRPEARN AINSSVSQAV GDALAEAQND PEVRAVVLTG AGDKSFCAGA DLKAISRGEN LFHPEHPEYG FAGYVSHFID KPTIAAVNGT ALGGGTELAL ASDLVVAEER AMFGLPEVKR GLIAGAGGVF RIIEQLPRKV ALELVYTGDP ISAADALKWG LINQVVPDGT VVDAALALAE RITCNAPLAV QASKRVAYGA EEGTVPAEQP SWKRTNREFV TLLKTEDAME GPLAFAQKRQ PVWKAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgaa accgcgccgg gcgctctcac cgagcgcaga ggcaatgtcc tgctcatcac catcaaccgg cccgaggcgc gcaacgccat caactcgtcg gtgagccagg ccgtcggcga cgcgctggcc gaagcgcaga acgaccccga ggttcgcgca gtggtcctca ccggtgcggg tgacaagtcg ttctgcgccg gtgcggacct caaggcgatc tcgcgtgggg agaacctctt ccaccccgag catcccgagt acggattcgc aggctacgtc agccatttca tcgacaagcc gaccatcgcc gcggtcaacg gcaccgctct cggtggcggt accgaactgg cgctggccag tgatctggtg gtcgccgagg agcgtgcgat gttcggtctg cccgaggtca agcgtggcct gatcgccggt gccggaggcg ttttccgcat catcgagcag ctgccgcgca aggtggcgct ggagttggtg tacaccggtg acccgatcag cgccgccgac gcgctgaagt ggggcctgat caaccaggtg gtgcccgacg gcaccgtcgt cgacgcggct ctggctctcg cggaacgcat cacgtgcaac gcaccgctgg ccgtgcaggc cagcaagcgc gtcgcctacg gcgccgagga gggcacggtg cccgccgagc agccgtcgtg gaagcgcacg aaccgcgagt tcgtcacgct gctgaaaacc gaagatgcca tggaaggtcc gctggctttc gcgcagaagc gccagcccgt ttggaaggcc aagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.01530.a.A1.PS00643
PURIFICATION DATe: 6/23/2010
CONCENTRATION: 74.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGXLEAQTQG PGSMVTETAP GALTERRGNV LLITINRPEA RNAINSSVSQ AVGDALAEAQ NDPEVRAVVL TGAGDKSFCA GADLKAISRG ENLFHPEHPE YGFAGYVSHF IDKPTIAAVN GTALGGGTEL ALASDLVVAE ERAMFGLPEV KRGLIAGAGG VFRIIEQLPR KVALELVYTG DPISAADALK WGLINQVVPD GTVVDAALAL AERITCNAPL AVQASKRVAY GAEEGTVPAE QPSWKRTNRE FVTLLKTEDA MEGPLAFAQK RQPVWKA
Validated NT Sequence
cttggccttc caaacgggct ggcgcttctg cgcgaaagcc agcggacctt ccatggcatc ttcggttttc agcagcgtga cgaactcgcg gttcgtgcgc ttccacgacg gctgctcggc gggcaccgtg ccctcctcgg cgccgtaggc gacgcgcttg ctggcctgca cggccagcgg tgcgttgcac gtgatgcgtt ccgcgagagc cagagccgcg tcgacgacgg tgccgtcggg caccacctgg ttgatcaggc cccacttcag cgcgtcggcg gcgctgatcg ggtcaccggt gtacaccaac tccagcgcca ccttgcgcgg cagctgctcg atgatgcgga aaacgcctcc ggcaccggcg atcaggccac gcttgacctc gggcagaccg aacatcgcac gctcctcggc gaccaccaga tcactggcca gcgccagttc ggtaccgcca ccgagagcgg tgccgttgac cgcggcgatg gtcggcttgt cgatgaaatg gctgacgtag cctgcgaatc cgtactcggg atgctcgggg tggaagaggt tctccccacg cgagatcgcc ttgaggtccg caccggcgca gaacgacttg tcacccgcac cggtgaggac cactgcgcga acctcggggt cgttctgcgc ttcggccagc gcgtcgccga cggcctggct caccgacgag ttgatggcgt tgcgcgcctc gggccggttg atggtgatga gcaggacatt gcctctgcgc tcggtgagag cgcccggcgc ggtttcngtc accatcgaac caggaccctg ggtctgagct tccagggnac ccatatgatg gtgatggtga tgagccnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTETAPGA LTERRGNVLL ITINRPEARN AINSSVSQAV GDALAEAQND PEVRAVVLTG AGDKSFCAGA DLKAISRGEN LFHPEHPEYG FAGYVSHFID KPTIAAVNGT ALGGGTELAL ASDLVVAEER AMFGLPEVKR GLIAGAGGVF RIIEQLPRKV ALELVYTGDP ISAADALKWG LINQVVPDGT VVDAALALAE RITCNAPLAV QASKRVAYGA EEGTVPAEQP SWKRTNREFV TLLKTEDAME GPLAFAQKRQ PVWKAK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGAA ACCGCGCCGG GCGCTCTCAC CGAGCGCAGA GGCAATGTCC TGCTCATCAC CATCAACCGG CCCGAGGCGC GCAACGCCAT CAACTCGTCG GTGAGCCAGG CCGTCGGCGA CGCGCTGGCC GAAGCGCAGA ACGACCCCGA GGTTCGCGCA GTGGTCCTCA CCGGTGCGGG TGACAAGTCG TTCTGCGCCG GTGCGGACCT CAAGGCGATC TCGCGTGGGG AGAACCTCTT CCACCCCGAG CATCCCGAGT ACGGATTCGC AGGCTACGTC AGCCATTTCA TCGACAAGCC GACCATCGCC GCGGTCAACG GCACCGCTCT CGGTGGCGGT ACCGAACTGG CGCTGGCCAG TGATCTGGTG GTCGCCGAGG AGCGTGCGAT GTTCGGTCTG CCCGAGGTCA AGCGTGGCCT GATCGCCGGT GCCGGAGGCG TTTTCCGCAT CATCGAGCAG CTGCCGCGCA AGGTGGCGCT GGAGTTGGTG TACACCGGTG ACCCGATCAG CGCCGCCGAC GCGCTGAAGT GGGGCCTGAT CAACCAGGTG GTGCCCGACG GCACCGTCGT CGACGCGGCT CTGGCTCTCG CGGAACGCAT CACGTGCAAC GCACCGCTGG CCGTGCAGGC CAGCAAGCGC GTCGCCTACG GCGCCGAGGA GGGCACGGTG CCCGCCGAGC AGCCGTCGTG GAAGCGCACG AACCGCGAGT TCGTCACGCT GCTGAAAACC GAAGATGCCA TGGAAGGTCC GCTGGCTTTC GCGCAGAAGC GCCAGCCCGT TTGGAAGGCC AAGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA