SSGCID: Target Page

MythA.01530.c

Carnitinyl-CoA dehydratase (A0QUJ1 homolog)

CENTER ID:	MythA.01530.c
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01530.c.A2.GE29589	potential soluble domain		1	196

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.262
RefSeq:	WP_003923688.1
UniProt:	G7CBD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPEWKAHPMI VEVRRQDRIL VIAMNRPAKR NAVNKEMAEA LSAALDLMDD DDELWAGVLT GTREFFCAGT DLKDGADART ERGGEYGIIR RRRRKPLIAA VEGACFGGGM EIAFACDLVV AATTARFALP ESRRGLVPSS GALLRATRSL PIHLAKELMI TGAELSGERA HHFGLVNRIA EPGAALEAAI DLAEDVXXXX XXXXXXXXXX XXXXXXXXAR

NT Sequence

CTGCCGGAAT GGAAGGCGCA CCCGATGATC GTTGAGGTCC GGCGCCAGGA CCGGATCCTC GTCATCGCGA TGAATCGCCC GGCTAAACGC AACGCGGTGA ACAAGGAGAT GGCCGAGGCG CTCAGTGCCG CGCTGGATCT CATGGATGAC GACGACGAGC TGTGGGCGGG TGTGCTGACG GGCACCCGCG AGTTCTTCTG CGCCGGCACC GATCTGAAGG ACGGAGCGGA CGCGCGAACC GAGCGCGGCG GGGAGTACGG AATCATCCGC CGCCGTCGCC GGAAACCGTT GATCGCCGCG GTCGAGGGTG CCTGCTTCGG CGGTGGCATG GAGATCGCCT TCGCGTGCGA CCTGGTCGTC GCGGCCACGA CGGCGCGCTT TGCCCTGCCC GAGTCACGGC GTGGTCTGGT GCCGTCGTCG GGCGCCCTGC TCCGGGCGAC GCGGTCGCTG CCCATTCATC TGGCCAAAGA ATTGATGATC ACCGGTGCGG AGTTGTCCGG CGAACGGGCT CACCATTTCG GTCTGGTCAA CCGGATAGCC GAACCGGGCG CCGCTCTGGA GGCGGCGATC GACCTCGCCG AGGACGTCTG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAGCTCGA

Details for MythA.01530.c.A2.GE29589

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	23kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLPEWKAHP MIVEVRRQDR ILVIAMNRPA KRNAVNKEMA EALSAALDLM DDDDELWAGV LTGTREFFCA GTDLKDGADA RTERGGEYGI IRRRRRKPLI AAVEGACFGG GMEIAFACDL VVAATTARFA LPESRRGLVP SSGALLRATR SLPIHLAKEL MITGAELSGE RAHHFGLVNR IAEPGAALEA AIDLAEDV

Validated NT Sequence

ttttntttan ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctgc cggaatggaa ggcgcacccg atgatcgttg aggtccggcg ccaggaccgg atcctcgtca tcgcgatgaa tcgcccggct aaacgcaacg cggtgaacaa ggagatggcc gaggcgctca gtgccgcgct ggatctcatg gatgacgacg acgagctgtg ggcgggtgtg ctgacgggca cccgcgagtt cttctgcgcc ggcaccgatc tgaaggacgg agcggacgcg cgaaccgagc gcggcgggga gtacggaatc atccgccgcc gtcgccggaa accgttgatc gccgcggtcg agggtgcctg cttcggcggt ggcatggaga tcgccttcgc gtgcgacctg gtcgtcgcgg ccacgacggc gcgctttgcc ctgcccgagt cacggcgtgg tctggtgccg tcgtcgggcg ccctgctccg ggcgacgcgg tcgctgccca ttcatctggc caaagaattg atgatcaccg gtgcggagtt gtccggcgaa cgggctcacc atttcggtct ggtcaaccgg atagccgaac cgggcgccgc tctggaggcg gcgatcgacc tcgccgagga cgtctaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttgnctgc tgccaccgnn a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgccggaa tggaaggcgc acccgatgat cgttgaggtc cggcgccagg accggatcct cgtcatcgcg atgaatcgcc cggctaaacg caacgcggtg aacaaggaga tggccgaggc gctcagtgcc gcgctggatc tcatggatga cgacgacgag ctgtgggcgg gtgtgctgac gggcacccgc gagttcttct gcgccggcac cgatctgaag gacggagcgg acgcgcgaac cgagcgcggc ggggagtacg gaatcatccg ccgccgtcgc cggaaaccgt tgatcgccgc ggtcgagggt gcctgcttcg gcggtggcat ggagatcgcc ttcgcgtgcg acctggtcgt cgcggccacg acggcgcgct ttgccctgcc cgagtcacgg cgtggtctgg tgccgtcgtc gggcgccctg ctccgggcga cgcggtcgct gcccattcat ctggccaaag aattgatgat caccggtgcg gagttgtccg gcgaacgggc tcaccatttc ggtctggtca accggatagc cgaaccgggc gccgctctgg aggcggcgat cgacctcgcc gaggacgtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a